SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene19640

Length: 1074 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene19640

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	elongation factor EF-2 [Arabidopsis thaliana] gb\|AAK32918.1\|AF367331_1 At1g56070/T6H22_13 [Arabidopsis thaliana] gb\|AAK96653.1\| elongation factor EF-2 [Arabidopsis thaliana] gb\|AAN31808.1\| putative elongation factor [Arabidopsis thaliana] gb\|AAN31864.1\| p	-	-	92%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	34%
Blast2go	elongation factor ef-2	-	-	95%

Source	GOs	Term	Type	Identity
Blast2go	response to cold	GO:0009409	Biological Process	95%
Blast2go	copper ion binding	GO:0005507	Molecular Function	95%
Blast2go	cytosol	GO:0005829	Cellular Component	95%
Blast2go	nucleolus	GO:0005730	Cellular Component	95%
Blast2go	vacuolar membrane	GO:0005774	Cellular Component	95%
Blast2go	chloroplast	GO:0009507	Cellular Component	95%
Blast2go	plasma membrane	GO:0005886	Cellular Component	95%

Source	InterPros	Term	Type	Identity
Sma3	GPCR, rhodopsin-like, 7TM	IPR000276	-	-
Sma3	Translation elongation factor EFG/EF2, C-terminal	IPR000640	-	-
Sma3	Protein synthesis factor, GTP-binding	IPR000795	-	-
Sma3	Translation elongation factor EFTu/EF1A, domain 2	IPR004161	-	-
Sma3	Small GTP-binding protein domain	IPR005225	-	-
Sma3	Translation elongation factor EFG/EF2, domain IV	IPR005517	-	-
Sma3	RNA polymerase I specific transcription initiation factor RRN3	IPR007991	-	-
Sma3	TonB box, conserved site	IPR010916	-	-
Sma3	Ribosomal protein S5 domain 2-type fold, subgroup	IPR014721	-	-

Full-Lengther Next Prediction

Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: B8LKZ4

Fln msg: Unexpected stop codon in the beginning of your sequence, Unexpected STOP codon at 3' end. Distance to subject end: 414 aas, your sequence is shorter than subject: 282 - 785

Fln protein: RRDLGCHSS*PWLSLIIVTKSNQLQRVQTNCS*TQVLIGIQE*GKMVKFTAEGLRTIMDRKHNIRNMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTRADEAERGITIKSTGISLYYEMEDAAVKNFKGDREGHEYLINLIDSPGHVDFSSEVTAALRITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFSRVIENANVIMATYEDPLLGDCQVYPRKRHSCILSRSAWVGIYPHKFCKNVCFKVWSG*

Protein Length: 283

Fln nts: CGTCGTGACCTTGGCTGTCACTCGTCGTGACCTTGGCTGTCACTCATTATCGTTACGAAGAGCAACCAGCTGCAAAGAGTACAAACGAATTGCAGCTAGACACAGGTGCTCATTGGTATTCAAGAGTAAGGCAAAATGGTGAAGTTCACAGCTGAAGGGCTTCGTACGATCATGGACAGAAAGCATAACATACGAAACATGTCTGTTATTGCCCATGTTGACCACGGAAAATCCACTCTCACTGATTCTTTGGTAGCAGCAGCTGGTATCATTGCGCAAGAAGTTGCCGGAGATGTCAGAATGACTGATACTCGGGCAGATGAAGCTGAACGTGGCATCACCATTAAGTCAACTGGAATTTCATTGTACTATGAGATGGAGGATGCAGCTGTGAAAAATTTCAAGGGGGATAGAGAAGGACATGAATATTTGATAAATCTGATTGATTCTCCAGGGCACGTTGACTTTTCTTCAGAAGTTACAGCAGCCCTGCGAATCACAGATGGTGCACTGGTTGTGGTAGATTGTATTGAAGGGGTTTGTGTCCAGACTGAGACTGTTCTTCGTCAAGCTCTTGGAGAAAGGATTAGGCCTGTTCTCACCGTGAACAAGATGGACAGGTGCTTTCTTGAGTTGCAGGTTGATGGAGAGGAAGCTTACCAAACCTTCTCACGTGTCATTGAAAATGCAAATGTGATTATGGCTACTTATGAAGATCCTCTTCTTGGTGACTGCCAAGTTTATCCTAGAAAAAGGCACAGTTGCATTCTCAGCAGGTCTGCATGGGTGGGCATTTACCCTCACAAATTTTGCAAAAATGTATGCTTCAAAGTTTGGAGTGGATGAAGCAAAGATGATGGAGAGGCTCTGGGGTGAAAACTTTTTTGATCCAGCTACCAAGAAATGGACTACCAAGAATACAGGTTCTGCAACTTGCAAAAGAGGATTTGTTCAATTCATATATGAACCTATTAAGCAAATTATCAATATTTGTATGAATGATCAAAAGGATAAGTTGTGGCCCATGTTGCAGAAATTGGGAGTTGTAATGATGATTTTCCCAACTGCAATAAG

Fln Alignment:

Contig19640___RNMSVIAHVDHGKSTLTDSLVAAAGIIAQ--EVAGDVRMTDTRADEAERGITIKSTGISLYYEMEDAAVKNFKGDREGHEYLINLIDSPGHVDFSSEVTAALRITDGALVVVDCIEGVCVQTETVLRQALGERIRPVLTVNKMDRCFLELQVDGEEAYQTFSRVIEN
B8LKZ4_______________RNIGIMAHIDAGKTTTTERILFYTGKNYKIGEVHDGTATMDWMEQEQERGITITSAATTCAW----------------NNHRINIIDTPGHVDFTLEVERALRVLDGSICLFDSVAGVEPQSETVWRQADRYGVPRICFVNKMDRL-------GANFFRTRDMIITN

SNPs (tot: 15)

Position	A %	C %	G %	T %	Probability
25	12			87	0.992554
26	12	87			0.992554
27			75	12	0.975346
28	12			87	0.992554
29	25		75		1.0
31		75	25		1.0
35			75	25	1.0
36			75	25	1.0
39	37		62		1.0
41		75		25	1.0
42	75			25	1.0
89	13	86			1.0
547		80		20	0.995493
658		10		90	0.90298
1005	80	20			0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain