SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene19102

Length: 1199 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene19102 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Unassigned protein	-	-	-
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	65%
Blast2go	conserved peptide upstream open reading frame 47	-	-	62%

Source	InterPros	Term	Type	e value	Identity
Sma3	Methyltransferase type 11	IPR013216	-	0.0	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LQR4

Fln msg: your sequence is shorter than subject: 329 - 528

Fln protein: PEHGKALQPYFFLSPVVALFKHSDVIHVNTSRSPNSQKTTVIALRKRPSKPLGLIKDYPRSKENISLTKLANQDHPPTKQNRSLTKLAEHGHPRSKENRSLAKLAEQDHPRSKQNRSLTKLAEQEHPRSKENISLTKLAEQDHPRSKQNRSLTKLAESLTLNHSNAVFLPTVKDISDRKGHIYVETGVNSNKTVNADAGWFLASYPKQNQTFDTYALNMSGFKPLPRRSLNSIKQVVRDTDSSSGSRERFDIAGWLSEMSVSDGDFVVVKMGFEHVTRDMTNMRGTGFMRLIDELFLQCSSELSEEVNGECLQFLQTLRDHDVFVHRS*

Protein Length: 330

Fln nts: GCCCGAACATGGGAAAGCCCTCCAACCTTATTTCTTCTTGAGCCCTGTGGTGGCCCTGTTCAAACACTCTGATGTCATTCATGTGAACACATCCAGGTCGCCTAATTCCCAGAAAACCACAGTGATTGCACTCCGTAAGAGGCCTTCTAAGCCGCTGGGTTTGATAAAGGACTATCCGCGCTCTAAGGAGAACATAAGCTTAACTAAACTGGCGAACCAGGACCATCCGCCCACTAAGCAGAACAGAAGCTTAACTAAACTGGCGGAGCATGGCCATCCGCGCTCTAAGGAGAACAGAAGCTTAGCTAAACTGGCGGAGCAGGACCATCCGCGCTCTAAGCAGAACAGAAGCTTAACTAAACTGGCGGAGCAGGAGCATCCGCGCTCTAAGGAGAACATAAGCTTAACTAAACTGGCAGAGCAGGACCATCCGCGCTCTAAGCAGAACAGAAGCTTAACTAAACTGGCGGAGTCCCTGACATTGAATCACAGCAATGCGGTTTTCTTGCCCACTGTAAAGGACATCAGCGACCGCAAAGGCCATATTTATGTGGAAACTGGGGTTAACAGTAACAAGACTGTCAATGCAGATGCAGGGTGGTTCTTGGCTTCTTATCCAAAGCAGAACCAGACATTTGATACTTATGCCCTCAATATGAGTGGATTCAAGCCTCTGCCACGTAGGTCTCTGAACTCAATAAAGCAGGTAGTTCGAGATACTGACAGCAGCAGCGGAAGCAGAGAGCGTTTCGACATTGCAGGATGGCTGAGTGAAATGAGTGTTAGCGATGGGGATTTTGTGGTTGTGAAAATGGGATTCGAACATGTGACCAGAGATATGACGAATATGAGAGGAACAGGATTCATGCGTCTCATTGACGAGCTCTTCCTGCAGTGTTCTTCGGAATTATCTGAAGAAGTTAATGGGGAATGCCTGCAATTCCTTCAGACTCTCAGAGATCATGATGTCTTCGTCCATCGATCGTAA___GGCGTCTCAGGTAATCGCTATTTCTATGGGCAGAATATTATCATACCGTAATAGAATAAGTAAACTGGTTTTCTGGATTTGGCGGTCGCTGATCAATCATTGGCGAAAAAATCCGGTCAAAGCAATAATCACAAGCATATGACTATTTGGTGTTAAACGTTATCTATTTTGGGAAACAATAAGCATCTTGTTACATATTAAGTTTTCTGTA

Fln Alignment:

Contig19102___PEHGKALQPYFFLSPVVALFKHSDVIHVNTSRSPNSQKTTVIALRKRPSKPLGLIKDYPRSKENISLTKLANQDHPPTKQNRSLTKLAEHGHPRSKENRSLAKLAE-QDHPRSKQNRSLTKLAEQEHPRSKENISLTKLAEQDHPRSKQNRSLTKLAESLTLNHSNAVFLPTVKDISDRKGHIYVETGVNS----NKTVNADAGWFLASYPKQNQTFDTYALN----MSGFKPLPRRSLNSIKQVVRDTDSSSGSRERFDIAGWLSEMSVSDGDFVVVKMGFEHVTRDMTNMRGTGFMRLIDELFLQCSSELSEEVNGECLQFLQTLRDHDVFVHR
B8LQR4_______________PNMGKPSNLMHSLSPVVALFKCSDVVHVTTSGLPNSQETTVIALRKRPSGV--------------------------SKQNRSLTKRPE---PKSMNHSNAIHLPKVKDYPRSKQNGSLTKLAEPRTMNRSNAIHLPKV--KDNPRSKQNRSLTKLAEPLTMNHSNAIYLPIVKDISDHKRHIYVEIGGNSQSKVNNTVNADAGWFLASYPKQNQTFDTYALNSHANMSGSESLPRRSLNSIKQVVGD-NGSSRSRERFDIGGWLSEMSVSDEDFVVVKMGFEHVTREMINKRGTGFMRLIDELFLQCSSELSDKVHMECLQFLQTLRDNGVFVHR

SNPs (tot: 34)

Position	A %	C %	G %	T %	Probability
318		37		62	1.0
333		87		12	0.992554
342		12		87	0.992554
343	12	12	75		0.975346
344	12			87	0.992554
363	71		28		1.0
523			75	25	0.996473
528	75			25	0.996473
530	25		75		0.996473
532			25	75	0.996473
533		25		75	0.996473
534		25	75		0.996473
537			25	75	0.996473
538		75		25	0.996473
542	25			75	0.996473
544	75		25		0.996473
602	83	16			0.994512
605	16	83			0.994512
662		60	40		1.0
682		20		80	0.995493
683		20		80	0.995493
684		20		80	0.995493
686		80		20	0.995493
701		25		75	0.996473
702		50	50		1.0
835		83	16		0.994512
836			83	16	0.994512
869	16	83			0.994512
880		50	50		1.0
883		50		50	1.0
897		50		50	1.0
912		50	50		1.0
1025		25	75		0.996473
1026		25	75		0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain