SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene18409

Length: 2426 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene18409
ATCGACACCTCCGCTGCTCGGAGAGCTCCAAATCGACATAAAAATCTCTACATTGGCCTCCAAATATCCTAAATGCAGAGGGTTTTATCACCATTATACGTGTTACCTTCGTTGACATTCGCTCTTCCAGGCGTTTCTGAGCTTAATTTTTAAGCATATAGGGCATTAACAGACCTCGAATTGATACCTTCAAGCTAATGGCGCAGCTGATGCAAAATCCACTTTCGATTCACAAATCCCCGCTCCAGGTTCGAATCCCGGCGCTTTCCTCTGTTAAAAGCCTTCAATGGCGGCCACCAAAGCCCAGGCAAAAGCTACGGATTAATAAGATCTCCGCAGTACTGGAAACCGGTAAATCTCTAGTGATTTCAGAGCCAGAGATCGCAAATAATAATAACGGTTCTTCTAATGGCGTTATGGTCAAGAAAAGTGCCGCAATGGCGCCATCGGTTATAGAGGTGGACAGCGTAACGGAGGCTGACTTGAAGGAAAACGGGTTTCGAAGTACGAAAAGGACGAAGCTGGTGTGCACGATTGGGCCCGCTTCCTGTAGTTTCGAACAGCTTGAAGCTCTGGCCATGGAAGGGATGAATGTAGCGAGGATTAATATGTGCCACGGTACCAGGGATTGGCATCACAATGTTATCAGGAATGTGAGGAAATTGAACGAAGAGAAGGGTTATGCAGTGGCTGTTATGATGGATACAGAGGGGGCTGAGATTCATATGGGGGATCTTGGAGGCGCTGCATCAGTTAAAGCCGAGGACGGTGAAGTTTGGACTTTCAGTGTTCGTGCTTTCAATGGGTCTCTTCCACCTCACACAATCCAAGTCAATTACGATGGCTTTGCAGAAGATGTCAAAGTTGGCGATGAACTAGTTGTTGATGGTGGTATGGTAAGGTTTGAGGTGATTGAGAAACAAGGTCCAGATGTGAAGTGCCAATGTACAGATCCTGGTTTGCTATTACCACGTGCTAACCTTACATTTTGGAGGCAGGGCAGATTGGTGCGTGAGAGGAATGCTATGCTTCCCACAATTTCTTCCAAGGATTGGCTTGATATAGATTTTGGAATCGCAGAGGGTGTTGATTTCATTGCAATATCTTTTGTGAAATCAGCAGAAGTAATCAAACATTTGAAAAGCTATATTGCTGCTCGATCTCGTGATAGTGATATTGGTGTCATTGCTAAAATTGAAAGCATTGATTCTCTCAAGAATCTAGAAGATATCATTAAATCATCTGATGGTGCCATGGTGGCAAGAGGGGATCTTGGTGCACAAATTCCACTTGAGCAAGTTCCAGCTGTCCAGCAGCATGTAGTCCAGCTATGCAGGCAACTCAACAAGCCAGTTATTGTTGCCTCTCAGCTACTTGAGTCAATGATAGAGTATCCTATACCAACAAGAGCTGAGGTTGCTGATGTGACAGAAGCAGTAAGGCAGCGTGCTGATGCTTTGATGCTCTCTGGTGAGTCAGCCATGGGTCAATTTCCAGAAAAAGCTTTAGCTGTTCTCAGAAGTGTAAGCCTGCGCATTGAATATTGGTGTCGGGAAGAAAAGAAGCACGAGTCTATGTACCTTCCAGACATTGCTACATCTCTTTCGGATAGTGTTTCTGAAGCAATTTGTAATTCTGCTGCTCATATGGCCAACAACTTGGAAGTGGATGCAATCTTTGTGTACACAAGACAAGGTTACATGGGTTCTCTTTTGTCACGATGTCGACCAGATTGCCCAATTTTTGCCTTCACCAGCTCAACAAGTGTGCGACGACGCATGAATCTTCAATGGGGCCTCATTCCATTTAGATTGGACTTATCAGATGATATGGAGAGCAATATCAATCGAACTTTCGCTTTGCTAAAAGCTAGAGGCATGATCAAATCAGGTGACTTGGTGATTGCGGTATCAGACATGTTGCAGTCTATTCAGGTCATGAAAGTGCCATGAGGTGCTTGTCACCAGTATGGTCTTAGCCTTGGGCAATCCTACAATTGTTCAGATCTCAATCTTGTTATGGCCTTTTTTATTTCATTGCCATTAGGTATATCTTCTTCTCATAGTTGACTAGTTCATTAGTGTTGTCCATTTCTCAAGTGGGTAGGTGGTAAATTAACTAAACGAGATTTCTAATTCAATGTTTGACATATATGATTTTATATGTAGCCGTTGAGCTGCCAGCTCTTTAATTTGTCATTTGTCGTCCCTCAATTTAGAAAACTAGAGCTCTAGCGTTCTTTTGTAGGACACTCATTTTTGGAGAGTTGTCTTGGTTTAATCTTCTTTGTTGCACCCATTTTAGTAAAAGTTTAAAGCATCAAAGGGGACTGTATTATTATTTTTCATTCTCCAAAATCGTTTATTCATTATTTAAAGCTGGGACCGAAGAGCTGAGCATCTTATACCAAGAAACTTTACCGACCT

Ace file of the UniGene sp_v2.0_unigene18409 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Pyruvate kinase isozyme A, chloroplastic n=3 Tax=Ricinus communis RepID=KPYA_RICCO	-	-	84%
FL-Next	sp=Pyruvate kinase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	43%
Blast2go	pyruvate kinase	-	-	90%

Source	ECs	Term	Type	e value	Identity
Blast2go		EC:2.7.1.4-	-	0.0	90%

Source	GOs	Term	Type	Identity
Blast2go	lipid metabolic process	GO:0006629	Biological Process	90%
Blast2go	response to cadmium ion	GO:0046686	Biological Process	90%
Blast2go	pyruvate kinase activity	GO:0004743	Molecular Function	90%
Blast2go	chloroplast stroma	GO:0009570	Cellular Component	90%

Source	InterPros	Term	Type	Identity
Sma3	Pyruvate kinase	IPR001697	-	-
Sma3	Pyruvate/Phosphoenolpyruvate kinase	IPR015813	-	-
Sma3	Pyruvate kinase, alpha/beta	IPR015794	-	-
Sma3	Pyruvate kinase, active site	IPR018209	-	-
Sma3	Pyruvate kinase, barrel	IPR015793	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LM06

Fln msg:

Fln protein: MAQLMQNPLSIHKSPLQVRIPALSSVKSLQWRPPKPRQKLRINKISAVLETGKSLVISEPEIANNNNGSSNGVMVKKSAAMAPSVIEVDSVTEADLKENGFRSTKRTKLVCTIGPASCSFEQLEALAMEGMNVARINMCHGTRDWHHNVIRNVRKLNEEKGYAVAVMMDTEGAEIHMGDLGGAASVKAEDGEVWTFSVRAFNGSLPPHTIQVNYDGFAEDVKVGDELVVDGGMVRFEVIEKQGPDVKCQCTDPGLLLPRANLTFWRQGRLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAISFVKSAEVIKHLKSYIAARSRDSDIGVIAKIESIDSLKNLEDIIKSSDGAMVARGDLGAQIPLEQVPAVQQHVVQLCRQLNKPVIVASQLLESMIEYPIPTRAEVADVTEAVRQRADALMLSGESAMGQFPEKALAVLRSVSLRIEYWCREEKKHESMYLPDIATSLSDSVSEAICNSAAHMANNLEVDAIFVYTRQGYMGSLLSRCRPDCPIFAFTSSTSVRRRMNLQWGLIPFRLDLSDDMESNINRTFALLKARGMIKSGDLVIAVSDMLQSIQVMKVP*

Protein Length: 586

Fln nts: ATCGACACCTCCGCTGCTCGGAGAGCTCCAAATCGACATAAAAATCTCTACATTGGCCTCCAAATATCCTAAATGCAGAGGGTTTTATCACCATTATACGTGTTACCTTCGTTGACATTCGCTCTTCCAGGCGTTTCTGAGCTTAATTTTTAAGCATATAGGGCATTAACAGACCTCGAATTGATACCTTCAAGCTA_-_ATGGCGCAGCTGATGCAAAATCCACTTTCGATTCACAAATCCCCGCTCCAGGTTCGAATCCCGGCGCTTTCCTCTGTTAAAAGCCTTCAATGGCGGCCACCAAAGCCCAGGCAAAAGCTACGGATTAATAAGATCTCCGCAGTACTGGAAACCGGTAAATCTCTAGTGATTTCAGAGCCAGAGATCGCAAATAATAATAACGGTTCTTCTAATGGCGTTATGGTCAAGAAAAGTGCCGCAATGGCGCCATCGGTTATAGAGGTGGACAGCGTAACGGAGGCTGACTTGAAGGAAAACGGGTTTCGAAGTACGAAAAGGACGAAGCTGGTGTGCACGATTGGGCCCGCTTCCTGTAGTTTCGAACAGCTTGAAGCTCTGGCCATGGAAGGGATGAATGTAGCGAGGATTAATATGTGCCACGGTACCAGGGATTGGCATCACAATGTTATCAGGAATGTGAGGAAATTGAACGAAGAGAAGGGTTATGCAGTGGCTGTTATGATGGATACAGAGGGGGCTGAGATTCATATGGGGGATCTTGGAGGCGCTGCATCAGTTAAAGCCGAGGACGGTGAAGTTTGGACTTTCAGTGTTCGTGCTTTCAATGGGTCTCTTCCACCTCACACAATCCAAGTCAATTACGATGGCTTTGCAGAAGATGTCAAAGTTGGCGATGAACTAGTTGTTGATGGTGGTATGGTAAGGTTTGAGGTGATTGAGAAACAAGGTCCAGATGTGAAGTGCCAATGTACAGATCCTGGTTTGCTATTACCACGTGCTAACCTTACATTTTGGAGGCAGGGCAGATTGGTGCGTGAGAGGAATGCTATGCTTCCCACAATTTCTTCCAAGGATTGGCTTGATATAGATTTTGGAATCGCAGAGGGTGTTGATTTCATTGCAATATCTTTTGTGAAATCAGCAGAAGTAATCAAACATTTGAAAAGCTATATTGCTGCTCGATCTCGTGATAGTGATATTGGTGTCATTGCTAAAATTGAAAGCATTGATTCTCTCAAGAATCTAGAAGATATCATTAAATCATCTGATGGTGCCATGGTGGCAAGAGGGGATCTTGGTGCACAAATTCCACTTGAGCAAGTTCCAGCTGTCCAGCAGCATGTAGTCCAGCTATGCAGGCAACTCAACAAGCCAGTTATTGTTGCCTCTCAGCTACTTGAGTCAATGATAGAGTATCCTATACCAACAAGAGCTGAGGTTGCTGATGTGACAGAAGCAGTAAGGCAGCGTGCTGATGCTTTGATGCTCTCTGGTGAGTCAGCCATGGGTCAATTTCCAGAAAAAGCTTTAGCTGTTCTCAGAAGTGTAAGCCTGCGCATTGAATATTGGTGTCGGGAAGAAAAGAAGCACGAGTCTATGTACCTTCCAGACATTGCTACATCTCTTTCGGATAGTGTTTCTGAAGCAATTTGTAATTCTGCTGCTCATATGGCCAACAACTTGGAAGTGGATGCAATCTTTGTGTACACAAGACAAGGTTACATGGGTTCTCTTTTGTCACGATGTCGACCAGATTGCCCAATTTTTGCCTTCACCAGCTCAACAAGTGTGCGACGACGCATGAATCTTCAATGGGGCCTCATTCCATTTAGATTGGACTTATCAGATGATATGGAGAGCAATATCAATCGAACTTTCGCTTTGCTAAAAGCTAGAGGCATGATCAAATCAGGTGACTTGGTGATTGCGGTATCAGACATGTTGCAGTCTATTCAGGTCATGAAAGTGCCATGA___GGTGCTTGTCACCAGTATGGTCTTAGCCTTGGGCAATCCTACAATTGTTCAGATCTCAATCTTGTTATGGCCTTTTTTATTTCATTGCCATTAGGTATATCTTCTTCTCATAGTTGACTAGTTCATTAGTGTTGTCCATTTCTCAAGTGGGTAGGTGGTAAATTAACTAAACGAGATTTCTAATTCAATGTTTGACATATATGATTTTATATGTAGCCGTTGAGCTGCCAGCTCTTTAATTTGTCATTTGTCGTCCCTCAATTTAGAAAACTAGAGCTCTAGCGTTCTTTTGTAGGACACTCATTTTTGGAGAGTTGTCTTGGTTTAATCTTCTTTGTTGCACCCATTTTAGTAAAAGTTTAAAGCATCAAAGGGGACTGTATTATTATTTTTCATTCTCCAAAATCGTTTATTCATTATTTAAAGCTGGGACCGAAGAGCTGAGCATCTTATACCAAGAAACTTTACCGACCT

Fln Alignment:

Contig18409___KRTKLVCTIGPASCSFEQLEALAMEGMNVARINMCHGTRDWHHNVIRNVRKLNEEKG-YAVAVMMDTEGAEIHMGDLGGAASVKAEDGEVWTFSVRAFNGSLPPHTIQVNYDGFAEDVKVGDELVVDGGMVRFEVIEKQGPDVKCQCTDPGLLLPRANLTFWRQGRLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAISFVKSAEVIKHLKSYIAARSRDSDIGVIAKIESIDSLKNLEDIIKSSDGAMVARGDLGAQIPLEQVPAVQQHVVQLCRQLNKPVIVASQLLESMIEYPIPTRAEVADVTEAVRQRADALMLSGESAMGQFPEKALAVLRSVSLRIEYWCREEKKHESMYLPDIATSLSDSVSEAICNSAAHMANNLEVDAIFVYTRQGYMGSLLSRCRPDCPIFAFTSSTSVRRRMNLQWGLIPFRLDLSDDMESNINRTFALLKARGMIKSGDLVIAVSDMLQSI
B8LM06_______________RKTKIVCTIGPSTSTPEMIHKLAEEGMNVARLNMSHGDHASHKKVIDLVKEYNAQSADNVIAIMLDTKGPEVRSGDL--PQPIMLGKGQEFIFTIKRGVGS--EKCVSVNYDGFVNDVEKGDTLLVDGGMMSLTVKSKTSDSVICEVIDGGELKSRRHLN-------VRGKSATLPSITDKDWEDIKFGVENEVDFYAVSFVKDAKVVHELKDYL--KSCNSDIQVTVKIESADSIPNLHSIISASDGAMVARGDLGAELPIEEVPLLQEDIIRVCCSMGKPVIVATNMLESMINHPTPTRAEVSDIAIAVREGADAVMLSGETAHGKYPLKAVKVMHTVALRTEATMLDEEPPAS-----FGRTSKEHMSEMFAFHATMMSNTLGT-SIVVFTRTGFMATLLSHYRPFGTIFAFTNEERVKQRLALYRGIRPIYMPFSNDAEETFSKALSLLQKLGMLKEGEQVALVQSGRQPI

SNPs (tot: 47)

Position	A %	C %	G %	T %	Probability
229		14		85	0.993533
353	87		12		0.992554
473		90	10		0.90298
474		90	10		0.90298
478		10		90	0.90298
480	10		90		0.90298
482	90		10		0.90298
497	88		11		0.923564
590	20	80			0.995493
591			20	80	0.995493
592		80		20	0.995493
593			20	80	0.995493
594	20	80			0.995493
597			20	80	0.995493
598	80	20			0.995493
599		20		80	0.995493
600	20	80			0.995493
602			20	80	0.995493
603		80	20		0.995493
605		20	80		0.995493
608			25	75	0.996473
610	75	25			0.996473
1699	14	85			0.993533
1772		11		88	0.923564
2196		22	77		0.999999
2203		22	77		0.999999
2204	87		12		0.992554
2205	87			12	0.992554
2281	85	14			0.993533
2296		83		16	0.994512
2298	16	83			0.994512
2299		83	16		0.994512
2305		16	83		0.994512
2309		16		33	1.0
2310	16		83		0.994512
2312	50		50		1.0
2314			50	50	1.0
2315	33			66	1.0
2316		50	50		1.0
2318	50		33		1.0
2320		33	66		1.0
2322	50	16		33	1.0
2323			50	50	1.0
2324			40	20	1.0
2326	60	40			1.0
2327	40		60		1.0
2332			40	60	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain