SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene18265

Length: 1468 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene18265 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	RecName: Full=Alpha-galactosidase; AltName: Full=Alpha-D-galactoside galactohydrolase; AltName: Full=Melibiase; Flags: Precursor emb\|CAA32772.1\| alpha-galactosidase preproprotein [Cyamopsis tetragonoloba]	-	-	60%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	82%
Blast2go	alpha-galactosidase alpha-n-	-	-	76%

Source	InterPros	Term	Type	Identity
Sma3	Glycoside hydrolase, clan GH-D	IPR000111	-	-
Sma3	WW/Rsp5/WWP	IPR001202	-	-
Sma3	Glycoside hydrolase, family 27	IPR002241	-	-
Sma3	Glycoside hydrolase, family 2, N-terminal	IPR006104	-	-
Sma3	Putative Ig	IPR008009	-	-
Sma3	Glycosyl hydrolase, family 13, all-beta	IPR013780	-	-
Sma3	Aldolase-type TIM barrel	IPR013785	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NWJ8

Fln msg: Separated hits, possible frame ERROR between 833 and 836,

Fln protein: MEFRYGCFICAVLLALICSAVTCGAENRTLGRRLLQNALAQTPPMGWNSWNHYGCNINENIIRETADALVSNGLSKLGYIYVNIDDCWAEPNRDQNGRLVASASKFPSGIKALADYVHSKGLKLGIYSDAGYYTCQKQPGSLGHEEVDANTFAFWGVDFLKYDNCYNDGSKPESRYSKMHDALLRTQWPIFYSVCEWGLDVPATWARQIGSSWRTTQDIEDKWESIVSHADQNNEYASFAGPGGWNxxDPDMLEVGNGKMTTEEYASHFSIWALMKSPLLIGCDVTSMDKKTYGILSNSEVIAVNQDPLGVQGTKVSKQVDLEVWAGPLSNNSVVVVLWNKSNSRATITAKWEDIGLNRSALFSVRNLWTHRELEENYQGSLTASVKPHGCMMYVLTSVSLQ*

Protein Length: 404

Fln nts: GCAGCACGCATCCTGTGCTATAATCTCTGTTCGTCGTGACCTTGGCTGTCACCATTGTCATTATTCATCACTACCTTCCTCGCTAGGAAACC_-_ATGGAATTTCGATATGGCTGTTTCATATGTGCCGTGCTTTTGGCCTTGATATGTTCTGCGGTGACCTGTGGTGCTGAGAATAGAACATTAGGACGGCGTTTGCTTCAGAATGCCCTTGCGCAGACCCCTCCTATGGGGTGGAATAGCTGGAATCACTACGGCTGCAACATCAATGAGAATATTATACGAGAAACAGCTGATGCGTTGGTATCAAATGGTCTCTCTAAGTTGGGGTATATCTACGTAAATATAGATGATTGTTGGGCAGAGCCTAATCGTGATCAAAATGGGAGATTGGTTGCAAGTGCTTCAAAGTTTCCCTCTGGTATCAAGGCTCTTGCTGATTATGTGCACAGCAAGGGTCTCAAACTGGGAATATATTCAGATGCTGGGTACTATACCTGTCAAAAGCAGCCAGGTTCCTTGGGACATGAGGAAGTGGATGCCAATACCTTCGCTTTCTGGGGAGTGGATTTTCTCAAATATGACAACTGTTACAATGATGGCTCTAAGCCTGAAAGCCGGTACTCAAAGATGCATGATGCACTTTTGAGAACTCAGTGGCCAATATTCTATTCGGTGTGTGAATGGGGGTTGGATGTTCCGGCCACCTGGGCTCGGCAAATTGGAAGCAGTTGGAGGACAACACAAGACATTGAGGACAAGTGGGAAAGCATTGTATCCCATGCAGATCAAAACAATGAATATGCAAGCTTTGCTGGTCCAGGTGGATGGAATGAxxxAGATCCAGATATGTTGGAAGTCGGAAATGGCAAAATGACTACCGAGGAATATGCTTCACATTTCAGCATCTGGGCTCTGATGAAGTCACCACTATTGATTGGATGTGATGTAACATCTATGGACAAGAAGACTTACGGAATCCTGAGTAACTCTGAGGTTATTGCAGTCAATCAAGATCCTTTGGGAGTACAGGGGACAAAAGTGAGCAAGCAAGTGGACCTGGAGGTATGGGCAGGTCCTCTATCTAACAACAGTGTAGTTGTGGTTCTATGGAATAAAAGCAACTCAAGAGCTACAATCACTGCCAAATGGGAAGATATTGGATTGAATCGCTCAGCTCTTTTCAGCGTTAGAAACTTATGGACGCACAGAGAATTGGAAGAAAATTACCAAGGTAGTCTCACAGCATCAGTGAAGCCTCATGGATGTATGATGTATGTTTTAACCAGCGTTTCTCTCCAGTGA___CCATCAAACTAATGATTGGAATTGCGAAAGTTCCAGATTATTTTTTCATATATGTTAAGATTTGGATAGTTTATGTTCTGATTATTGGTGGGTGATGCCTACTCAAAACATCACTTTCCATCCATTTTCTTAATTAATAAAATAGAGAAGAAAGTGAAAATGCAAAGG

Fln Alignment:

Contig18265___MEFRYGCFICAVLLALICSAVTCGAENRTLGRRLLQNALAQTPPMGWNSWNHYGCNINENIIRETADALVSNGLSKLGYIYVNIDDCWAEPNRDQNGRLVASASKFPSGIKALADYVHSKGLKLGIYSDAGYYTCQKQPGSLGHEEVDANTFAFWGVDFLKYDNCYNDGSKPESRYSKMHDALLRTQWPIFYSVCEWGLDVPATWARQIGSSWRTTQDIEDKWESIVSHADQNNEYASFAGPGGWNExDPDMLEVGNGKMTTEEYASHFSIWALMKSPLLIGCDVTSMDKKTYGILSNSEVIAVNQDPLGVQGTKVSKQVDLEVWAGPLXXXXXXXXXXXXXXSRATITAKWEDIGLNRSALFSVRNLWTHRELEENYQGSLTASVKPHGCMMYVLTSVS
A9NWJ8_______________MDFQYGCFVHAVLLAFACCAVTCGAQNTTSGRSLLQSGLAQTPPLGWNSWNHFGCHINEDVIRETADALVSTGLSKLGYQYVNIDDCWAELNRDSNGRLVASASRFPSGIKALADYVHSKGLKLGIYSDAGYYTCQKQPGSLGHEQVDADTFAYWGVDYLKYDNCYNDGSKPENRYPKMRDALLSTRRPIFYSLCEWGLDVPATWARQIGSSWRTTDDIEDKWESMISRADQNNEFAQYAGPGGWNDxDPDMLEVGNGNMTPEEYGSHFSIWALMKAPLLIGCDVTSMDKKTYGILSNSEVIAVNQDPLGVQGKKVNKLGDLEVWAGPLSNNRVAVVLLNRSNSRTIITAKWEDIGLNPSAVVRVRNLWRHRELEANHQGSLTASVKPHGCKMYVLTSVS

SNPs (tot: 22)

Position	A %	C %	G %	T %	Probability
327			23	76	1.0
398	15	84			0.999986
489	14			85	0.993533
537	14			85	0.993533
538		85		14	0.993533
541		14		85	0.993533
625	75	25			0.996473
636		66		33	0.997452
1059	75		25		0.996473
1108	85		14		0.993533
1389	66	33			1.0
1402		16	83		0.994512
1403		16		83	0.994512
1404		16	83		0.994512
1426		25	50		0.995533
1427	75	25			0.996473
1430			50	25	0.995533
1432		66		33	0.997452
1434	66			33	0.997452
1435	33		66		0.997452
1438	33	66			0.997452
1440		66	33		0.997452

Back

Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain