SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene17079

Length: 1510 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene17079 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	RecName: Full=Ribonucleoside-diphosphate reductase small chain; AltName: Full=Ribonucleoside-diphosphate reductase R2 subunit; AltName: Full=Ribonucleotide reductase small subunit emb\|CAA63194.1\| ribonucleotide reductase R2 [Nicotiana tabacum]	-	-	82%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	78%
Blast2go	protein	-	-	89%

Source	ECs	Term	Type	e value	Identity
Blast2go	Ribonucleoside-diphosphate reductase.	EC:1.17.4.1	-	0.0	89%

Source	KEGGs	Term	Identity
Blast2go	Purine metabolism	00230	89%
Blast2go	Pyrimidine metabolism	00240	89%
Blast2go	Glutathione metabolism	00480	89%
Blast2go	Metabolic pathways	01100	89%

Source	GOs	Term	Type	Identity
Blast2go	DNA replication	GO:0006260	Biological Process	89%
Blast2go	multicellular organismal development	GO:0007275	Biological Process	89%
Blast2go	regulation of cell cycle	GO:0051726	Biological Process	89%
Blast2go	programmed cell death	GO:0012501	Biological Process	89%
Blast2go	ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor	GO:0004748	Molecular Function	89%

Source	InterPros	Term	Type	e value	Identity
Sma3	Ribonucleotide reductase-related	IPR012348	-	0.0	-
Sma3	Ribonucleotide reductase	IPR000358	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5ACY5

Fln msg: your sequence is longer than subject: 329 - 289

Fln protein: MGVMAEEPLLVENPQRFCMFPIKYPEIWEMYKKAEASFWTAEEVDLSEDTQHWEALTGDEKHFITHVLAFFAASDGIVLENLAGRFMKEVQVPEARAFYGFQIAIENIHSEMYSLLLETYVKDPKEKNHLFNAIETVPCVAKKADWALNWIDSAESFAERIIAFACVEGIFFSGSFCAIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLNNKLSEDKVRKIVCDAVEIEREFVCDALPCDLVGMNSKLMSQYIDFVADRLLVALGCAKVYNAQNPFDWMELISLQGKTNFFEKRVGEYQKASVMSNLNGNQTHVFSLDEDF*

Protein Length: 330

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATATATTCCTCACATATGAACCAAAGCAATGGTGTGGAGTAGCCGGAAGAGCTAACTAGTTCAGTCCATCTGGATTGTGCCCTCTTCAACTCGCCTTATTGGAGGCTTCTTGTCCTATTTGGCGGATTTTCTATTTCGATCAAACAGAGACAGATTCATCAATCAGTAGGTCAATC_-_ATGGGTGTGATGGCAGAGGAGCCCTTGTTGGTAGAGAATCCCCAGAGGTTTTGTATGTTTCCCATTAAGTACCCTGAAATCTGGGAAATGTATAAGAAGGCAGAAGCTAGCTTCTGGACTGCGGAGGAAGTTGATCTCTCTGAGGATACCCAGCATTGGGAGGCATTGACTGGTGATGAGAAGCACTTCATCACTCACGTCCTTGCATTCTTTGCTGCCTCTGACGGCATTGTTCTCGAAAACCTGGCTGGGCGTTTCATGAAAGAAGTTCAAGTTCCAGAGGCTCGTGCATTTTATGGGTTCCAGATTGCCATTGAGAATATTCACTCAGAGATGTACAGTTTACTGTTGGAGACATATGTCAAAGATCCCAAAGAAAAGAATCACCTGTTCAATGCAATTGAGACAGTTCCTTGTGTGGCAAAGAAAGCCGATTGGGCTCTCAACTGGATAGACAGTGCTGAAAGCTTTGCAGAAAGGATTATTGCATTTGCTTGTGTTGAGGGTATTTTTTTCTCTGGCAGTTTTTGTGCAATCTTTTGGCTGAAGAAAAGGGGGCTGATGCCCGGTCTCACTTTTTCCAATGAACTCATTTCAAGGGATGAAGGCCTCCATTGTGATTTTGCATGCTTGCTCTACAGCTTACTGAACAACAAACTGAGTGAGGACAAAGTAAGAAAGATAGTTTGTGATGCTGTTGAGATTGAAAGAGAATTTGTATGTGATGCCCTTCCATGTGATCTTGTCGGAATGAATTCCAAGCTTATGAGTCAATATATTGACTTTGTTGCAGATAGATTGCTGGTTGCTTTGGGATGTGCAAAGGTTTATAATGCACAAAATCCTTTTGATTGGATGGAATTAATCTCTCTTCAGGGAAAGACAAATTTCTTTGAGAAAAGAGTAGGAGAATATCAGAAGGCTTCCGTTATGTCCAATCTAAATGGCAACCAGACTCACGTGTTTAGTTTAGATGAAGATTTTTGA___AAGCAGGTATCAAGTTGAAGTGGATATTCATCCAATCTCATTTAAGTGCTTGGAATTTTCCTTGATGTACTAAATGGGTTCTGACTTATCTATGTTCCAAGTCAGCATCATTGTATTTTCATCGATGGCTTGGAAATGCAACTTGTATTTGTATTCTAAATTGGCTGTCTTTTGGGTGGGCAGCTTTTCTAGCTCAATCTCAATCCAAATCTCCTTAATTTCATTTGTTACTTTTAATGGCAAAGTTGTATTGCAAAGGTCTGTTTATACTCTTTTCTTGAGCATTCAATAAAGAAGTGATAGTATTTATCACAACTTGTCCC

Fln Alignment:

Contig17079___EEVDLSEDTQHWEALTGDEKHFITHVLAFFAASDGIVLENLAGRFMKEVQVPEARAFYGFQIAIENIHSEMYSLLLETYVKDPKEKNHLFNAIETVPCVAKKADWALNWIDSAESFAERIIAFACVEGIFFSGSFCAIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLNNKLSEDKVRKIVCDAVEIEREFVCDALPCDLVGMNSKLMSQYIDFVADRLLVALGCAKVYNAQNPFDWMELISLQGKTNFFEKRVGEYQKASVMSNL-NGNQTHVFSLDEDF
D5ACY5_______________EEVDLSQDLYQWVNLTKNEKHFISHVLAFFAASDGIVLENLAGRFLKDVQIPEARAFYGFQIAIENIHSEMYSLLLETYIRDSKEKARLFNAINTIPCVARKAEWAMRWIQSSGSFAERIVSFACVEGIFFSGSFCAIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLLYSLLKSKLSEERVKAIVRDAVDIEREFVCEALSCDLVGMNATLMSQYIKFIADRLLVALGHGKMYHVENPFDWMDLISLQGKTNFFERRVGDYQKASIMSSISNAEGNHVFKMDEDF

SNPs (tot: 36)

Position	A %	C %	G %	T %	Probability
1057	88		11		0.923564
1145	85	14			0.993533
1201			83	16	0.994512
1202		83	16		0.994512
1206		83		16	0.994512
1207			16	83	0.994512
1208	16			83	0.994512
1210	16			83	0.994512
1214	20			80	0.995493
1215	20			80	0.995493
1216		80	20		0.995493
1217			20	80	0.995493
1220		20		80	0.995493
1223	80		20		0.995493
1264		75		25	0.996473
1291		62		37	1.0
1319		75		25	1.0
1379			75	25	1.0
1407			75	25	1.0
1420			26	73	1.0
1445			78	21	1.0
1449		75		25	1.0
1455	81	18			0.999994
1457		22		11	0.999999
1458	33	55	11		1.0
1460			12	87	0.992554
1464	16			83	0.994512
1465		16		83	0.994512
1466	16			83	0.994512
1470		16		83	0.994512
1475			16	83	0.994512
1477		33		66	1.0
1478		16	83		0.994512
1480		16	83		0.994512
1483	83	16			0.994512
1484			16	83	0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain