SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene16881

Length: 2184 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene16881
GTCTGACTGAAAACCAACGGAACTAGGACAGACATCGACGAATTTGAGGGTTGCCGATCAATTTGGTGGGAATCGACAAATATATATATATACGTCGTGACCGTCGTGACCTTGGCATGTCAAGAACAAGCTTGAGAGGAAGAATTAGGAACGATGGCGAAGTATAGTCCAGAGAATGGTTTGCGTGCAGGTTACTTTGTGGACGATGGGATGGAGCAAATGAAGCTCGATCCGAGGTTGCCTCTGTTGCAGGAGGCCGATCTCAAAGACAAAGTCGTTCTTCTCAGAGTCGATCACAATGTCGTCAAGAAAGGGAAAGTACTAGATCAGTACAGAATCGATGTGACTCTGCCAACTATGTACAACATAGTGGAGCGAGGTGGAAGGCTCATAATCATGAGTCACATTGGACGCCCGTACGATAAGAAGACAAAAACTATCTCTATACGTGAGGATGATTCTGTGGATGCTGTTGTTGCATATTTGCAACGCAAACTCCGCATCAAATTTGCAATTCCCCGTTTAAGTACTACTGAAAAAAATGGAATTCTGGATATTGATACCTCCATCAACTGGTTGATCCATGACCTTAAGATTCGGAAAATTGGTGGAATTTACCTACCCAATAGCCGATGGTTTGCGGGGGAGGAAGACAAAGGACAAAAGCTTGAAAGATTTGTCATTCAACTTTCGGCTCTAGCTGATGTATATGTCAATGATGCATTTGGCTCGTGGCAGCCTCATGGTTCCACGTACCATATTGCGAAATGCTTGCCCTCATATGCAGGATTGTGCATGCAGGATGAACTTTATCATGTGCAAGCAGTCTTGAATCCCACGAGGCCGTTTGTTGCAATTGTTGCTGGTGCAAAGTATGATACAAAGATTGGTCCATTAAATGAGATCTACAAGAAAGTTGATAAATTGGTTCTGGGTGGTCTCATATACAATACTTATCTATGTGCTAAATATCGAGTTAAAATTGCTGGCGTTGAGGATTCAGATATTGAACTTGCAAAAGAGCTGGTAGAACAGGATAAGACGCACAAAAAAATTGTAGAGATGCAGGTGCTTGTTGAATCTGATACTCTGGATGGTAGATTTGAAGGGCATTTTAGAACAATCAATATCAAGGACTTTGCTCCTGGCCAGACGTACAAGTATGTTGTTGATGTTGATCCCACCTCATTTGAGAAAGATGAGGTTGAACAAGCAATGAACTCAGCCAAGACAATATTTGTTAATGCTGTGATGGGACTCATGCCGCATTTCTGGCATGGCACAAGCAAAATGGATGAGGCGATTGATAAAAATCGCGCAGCTCAAAAATTCTTTGGAGGTGGTGATACCCTTCAGACTTTTAAAAGCCTTCATCCTCTGCTGTATGAGGCCGCATTGGACAATATCAAATACTATTTATTTACGGGAGGTGGTACTGTTTTGAAGGTTATTGAAGAGGGAGATCCAATGGGATTGGAGACTGTAAAGGCTCTTATTGACAACAAGGCAAACTATTTTAAGAGCATTCAAATGAATTGAAGCTAGCTTATGACACATGCTTTAGCGTGCAGAGTAAGGGGTTGATATCTCTCATAGGAATTGGTTAAAGACATATGCTGTTTGGTATAATACAGCCATCGTGCAGAGATAGCTGATTTAAGGCTTATATGGTAGATTTTTTGATAATTTAGTACTTTTATATTTGTAGAGGATTCCAAAGAATATAGCAGGAGAGTGTTATGTAATGTAGCTATAATGACCACAAGGGCGGCTGTGTGAGTCAGTATAAAACGTTAAAGCGTTCCTTCGCCAGTTCTTATCGCTAGTGTTTTGGGGACGTGTCAATAAAAATGTAAAACTGGTGTCATGCTACAAGACACCCCTTTCCTAGTTCTTGTTGCCTGACACATTGATGTACATAGATCCATCTTAATCCGAGTAAAACTTCTCACTGCTTAAATTTGGTCTCTCTGTGCAAAAATATGTAGTTGCATTAGTTACTACATTTGTGTTCCCAAGATATTTGGATGGCAGTCTATAAATTGTCTGCAAACTCTATGGAGACTATGTAGGGAATACAACAATTATTTGGAGTGACTATATATCTATGCTACCGTTCCACCTATGTGGCCAGAAAACAGAGATGTCACTTTCGGTTTTAAAAAATGTTTGTTGCAAGTATAC

Ace file of the UniGene sp_v2.0_unigene16881 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Phosphoglycerate kinase n=4 Tax=Picea sitchensis RepID=A9NW50_PICSI	-	-	79%
FL-Next	sp=Phosphoglycerate kinase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	79%
Blast2go	phosphoglycerate kinase	-	-	75%

Source	InterPros	Term	Type	Identity
Sma3	Phosphoglycerate kinase	IPR001576	-	-
Sma3	Phosphoglycerate kinase, C-terminal	IPR015901	-	-
Sma3	Phosphoglycerate kinase, N-terminal	IPR015824	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LLT2

Fln msg:

Fln protein: MAKYSPENGLRAGYFVDDGMEQMKLDPRLPLLQEADLKDKVVLLRVDHNVVKKGKVLDQYRIDVTLPTMYNIVERGGRLIIMSHIGRPYDKKTKTISIREDDSVDAVVAYLQRKLRIKFAIPRLSTTEKNGILDIDTSINWLIHDLKIRKIGGIYLPNSRWFAGEEDKGQKLERFVIQLSALADVYVNDAFGSWQPHGSTYHIAKCLPSYAGLCMQDELYHVQAVLNPTRPFVAIVAGAKYDTKIGPLNEIYKKVDKLVLGGLIYNTYLCAKYRVKIAGVEDSDIELAKELVEQDKTHKKIVEMQVLVESDTLDGRFEGHFRTINIKDFAPGQTYKYVVDVDPTSFEKDEVEQAMNSAKTIFVNAVMGLMPHFWHGTSKMDEAIDKNRAAQKFFGGGDTLQTFKSLHPLLYEAALDNIKYYLFTGGGTVLKVIEEGDPMGLETVKALIDNKANYFKSIQMN*

Protein Length: 463

Fln nts: GTCTGACTGAAAACCAACGGAACTAGGACAGACATCGACGAATTTGAGGGTTGCCGATCAATTTGGTGGGAATCGACAAATATATATATATACGTCGTGACCGTCGTGACCTTGGCATGTCAAGAACAAGCTTGAGAGGAAGAATTAGGAACG_-_ATGGCGAAGTATAGTCCAGAGAATGGTTTGCGTGCAGGTTACTTTGTGGACGATGGGATGGAGCAAATGAAGCTCGATCCGAGGTTGCCTCTGTTGCAGGAGGCCGATCTCAAAGACAAAGTCGTTCTTCTCAGAGTCGATCACAATGTCGTCAAGAAAGGGAAAGTACTAGATCAGTACAGAATCGATGTGACTCTGCCAACTATGTACAACATAGTGGAGCGAGGTGGAAGGCTCATAATCATGAGTCACATTGGACGCCCGTACGATAAGAAGACAAAAACTATCTCTATACGTGAGGATGATTCTGTGGATGCTGTTGTTGCATATTTGCAACGCAAACTCCGCATCAAATTTGCAATTCCCCGTTTAAGTACTACTGAAAAAAATGGAATTCTGGATATTGATACCTCCATCAACTGGTTGATCCATGACCTTAAGATTCGGAAAATTGGTGGAATTTACCTACCCAATAGCCGATGGTTTGCGGGGGAGGAAGACAAAGGACAAAAGCTTGAAAGATTTGTCATTCAACTTTCGGCTCTAGCTGATGTATATGTCAATGATGCATTTGGCTCGTGGCAGCCTCATGGTTCCACGTACCATATTGCGAAATGCTTGCCCTCATATGCAGGATTGTGCATGCAGGATGAACTTTATCATGTGCAAGCAGTCTTGAATCCCACGAGGCCGTTTGTTGCAATTGTTGCTGGTGCAAAGTATGATACAAAGATTGGTCCATTAAATGAGATCTACAAGAAAGTTGATAAATTGGTTCTGGGTGGTCTCATATACAATACTTATCTATGTGCTAAATATCGAGTTAAAATTGCTGGCGTTGAGGATTCAGATATTGAACTTGCAAAAGAGCTGGTAGAACAGGATAAGACGCACAAAAAAATTGTAGAGATGCAGGTGCTTGTTGAATCTGATACTCTGGATGGTAGATTTGAAGGGCATTTTAGAACAATCAATATCAAGGACTTTGCTCCTGGCCAGACGTACAAGTATGTTGTTGATGTTGATCCCACCTCATTTGAGAAAGATGAGGTTGAACAAGCAATGAACTCAGCCAAGACAATATTTGTTAATGCTGTGATGGGACTCATGCCGCATTTCTGGCATGGCACAAGCAAAATGGATGAGGCGATTGATAAAAATCGCGCAGCTCAAAAATTCTTTGGAGGTGGTGATACCCTTCAGACTTTTAAAAGCCTTCATCCTCTGCTGTATGAGGCCGCATTGGACAATATCAAATACTATTTATTTACGGGAGGTGGTACTGTTTTGAAGGTTATTGAAGAGGGAGATCCAATGGGATTGGAGACTGTAAAGGCTCTTATTGACAACAAGGCAAACTATTTTAAGAGCATTCAAATGAATTGA___AGCTAGCTTATGACACATGCTTTAGCGTGCAGAGTAAGGGGTTGATATCTCTCATAGGAATTGGTTAAAGACATATGCTGTTTGGTATAATACAGCCATCGTGCAGAGATAGCTGATTTAAGGCTTATATGGTAGATTTTTTGATAATTTAGTACTTTTATATTTGTAGAGGATTCCAAAGAATATAGCAGGAGAGTGTTATGTAATGTAGCTATAATGACCACAAGGGCGGCTGTGTGAGTCAGTATAAAACGTTAAAGCGTTCCTTCGCCAGTTCTTATCGCTAGTGTTTTGGGGACGTGTCAATAAAAATGTAAAACTGGTGTCATGCTACAAGACACCCCTTTCCTAGTTCTTGTTGCCTGACACATTGATGTACATAGATCCATCTTAATCCGAGTAAAACTTCTCACTGCTTAAATTTGGTCTCTCTGTGCAAAAATATGTAGTTGCATTAGTTACTACATTTGTGTTCCCAAGATATTTGGATGGCAGTCTATAAATTGTCTGCAAACTCTATGGAGACTATGTAGGGAATACAACAATTATTTGGAGTGACTATATATCTATGCTACCGTTCCACCTATGTGGCCAGAAAACAGAGATGTCACTTTCGGTTTTAAAAAATGTTTGTTGCAAGTATAC

Fln Alignment:

Contig16881___MAKYSPENGL-RAGYFVDDGMEQMKL-DPRLPLLQEADLKDKVVLLRVDHNVVKKGKVLDQYRIDVTLPTMYNIVERGGRLIIMSHIGRPYDKKTKTISIREDDSVDAVVAYLQRKLRIKFAIPRLSTTEKNGILDIDTSINWLIHDLKIRKIGGIYLPNSRWFAGEEDKGQKLERFVIQLSALADVYVNDAFGSWQPHGSTYHIAKCLPSYAGLCMQDELYHVQAVLNPTRPFVAIVAGAKYDTKIGPLNEIYKKVDKLVLGGLIYNTYLCAKYRVKIAGVEDSDIELAKELVEQDKTHKKIVEMQVLVESDTLDGRFEGHFRTINIKDFAPGQTYKYVVDVDPTSFEKDEVEQAMNSAKTIFVNAVMGLMPHFWHGTSKMDEAIDKNRAAQKFFGGGDTLQTFKSLHPLLYEAALDNIKYYLFTGGGTVLKVIEEGDPMGLETVKALIDNK
B8LLT2_______________MAPYGQENGLTRIGYSVDDGLENDKIADPRLPLLPDADLKDKIVLLRVDHNVVKKGEILDQYRIDVTLPTLYNITERGGRPILMTHVGRPYDKKNKTVTIRKDESVDAIVAYLQRKLRIKFVVPKFNYDEKDGILDIDTSINWLITDLRKRKIGGIYLPNTRWFAGEEDKGEKLERFAIQLAGLADVFVNDAFGSWQPHGSTYHITKYLPSYAGLCMQDELYHVEAVLNPTRPFIAIVAGSKYDTKIGPLNQIYKKVDKLVLGGVIYNTYLCAKYGVKIAGVQDSDIELAKELVEQDKTQGKIVEMDILVETDTLSGRFEGKYRAVNVKDFAPGKTYNYIVDIDPLSLESEDVLQALNSAKTIFVNAVMGFMPNFWHGTSKLDEVIDNNQSAKKYFGGGDTLQAFKSLHPNLYQAALDNTNYYLFTGGGTVLKVIEEGDSMGLDTVRALIENK

SNPs (tot: 43)

Position	A %	C %	G %	T %	Probability
1687		5	94		0.998744
1893			8	91	1.0
1894		91		8	1.0
1898			8	91	1.0
1900		8		91	1.0
1901	8		91		1.0
1905	91		8		1.0
1906			91	8	1.0
1907	91	8			1.0
1912		58		41	1.0
1913			8	91	1.0
1930	4	95			0.996252
1932			4	95	0.996252
2033	5	94			0.998397
2036			5	94	0.998744
2042			5	94	0.998894
2046	5			94	0.998894
2048		94	5		0.998894
2049	2	5		91	0.995912
2059			60	3	1.0
2061	7		39	53	1.0
2062	39	53	7		1.0
2063			39	60	1.0
2066		19	80		1.0
2067	68	20	12		1.0
2068	12	72		4	1.0
2070	4		4	44	1.0
2071	5	15	60		1.0
2072	5	85		10	0.999793
2073	5	75	10	5	0.999571
2074	80	15	5		1.0
2075	80	5	10	5	0.99967
2076		10	70	20	1.0
2077		11	88		0.999937
2078	16	5		77	1.0
2081			16	11	1.0
2083		17	82		1.0
2085	17	82			1.0
2087			75	25	0.996473
2089		75		25	0.996473
2092			75	25	0.996473
2094		75		25	0.996473
2095	25		75		0.996473

SSRs (tot: 1)

Start position	End position	Sequence	Length
2093	2105	TA TA TA TA TA TA T	13

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain