SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene16344

Length: 1743 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene16344 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	NADPH2:quinone reductase [Arabidopsis thaliana] gb\|ABM06041.1\| At5g61510 [Arabidopsis thaliana] gb\|AED97477.1\| NADPH2:quinone reductase [Arabidopsis thaliana]	-	-	77%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	90%
Blast2go	quinone oxidoreductase 1	-	-	86%

Source	GOs	Term	Type	e value	Identity
Blast2go	response to high light intensity	GO:0009644	Biological Process	0.0	86%
Blast2go	cell differentiation	GO:0030154	Biological Process	0.0	86%

Source	InterPros	Term	Type	Identity
Sma3	Alcohol dehydrogenase superfamily, zinc-type	IPR002085	-	-
Sma3	Quinone oxidoreductase/zeta-crystallin, conserved site	IPR002364	-	-
Sma3	Alcohol dehydrogenase, C-terminal	IPR013149	-	-
Sma3	Alcohol dehydrogenase GroES-like	IPR013154	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: C0PSW7

Fln msg: Separated hits, possible frame ERROR between 1004 and 1079,

Fln protein: MLLKPRFTTPCAWRESDQLTVLSVSSRLL*TNFHPQSKPIRSFKAFASMVKAIRVHELGGAEVLKWEDVEVPEPGAGEILVKHKAIGVNFIDIYYRKGVYKTEKLPFVPGMEAVGVVTAVGPGLTGKNVGDLVAYAGNPMGSYAEEQILPANQVVPFPSTIDPIVGAAVMLKGMTVQYLLRSCFKVEPGHTILVHAAAGGVGSLLCQWGKALGATVIGTVSNEEKAAQAAEDGCHHPIIYTKENFVERVKEITKGQGVHVVYDSVGKDTFxxxxxxxxxxxxxxxxxxxxxxxxxxTFQGSLECLAVRGYMVSFGQSSGMVDPFPMSVLASKSLFLTRPSMMHYIATRDELLRCAGEVFSAVANGILKVRVTKTYPLSEAAKAHADLEARKTTGSIVLIPDNS*

Protein Length: 405

Fln nts: ATGGGGGTTGGGTGTTTTCCTAACGTTCATTATACGTGAGATTTCGAGAGTTGATCATCGAATTCACCCCGAGGGAACCGCTTGCTTATGAAATGCCGAGCTCGGTGCTTGAATTTTGATCAAGCGCGCATGTCTCCAGTGAAAGCAACCCATGAGCGTCCAGTGAATTCCACAATGGTTGATCTCAAATA_-_ATGCTGTTGAAGCCTCGTTTCACCACACCGTGCGCTTGGAGAGAATCAGATCAACTGACTGTTCTTTCAGTTTCTTCGAGATTACTTTGAACGAATTTTCATCCCCAGTCGAAACCTATTAGATCTTTTAAAGCATTCGCAAGCATGGTGAAGGCTATCAGAGTACACGAGCTCGGAGGTGCAGAGGTATTAAAGTGGGAGGATGTGGAGGTACCAGAGCCTGGAGCGGGGGAAATACTTGTTAAACATAAAGCTATTGGCGTAAATTTTATTGATATATATTATCGCAAGGGGGTTTACAAAACTGAAAAGCTACCTTTCGTTCCTGGAATGGAAGCAGTGGGAGTGGTAACTGCAGTAGGACCAGGATTAACAGGAAAAAATGTGGGGGATTTAGTTGCCTATGCGGGGAATCCTATGGGTTCATATGCTGAAGAGCAAATTCTTCCTGCAAATCAGGTTGTGCCATTTCCATCTACAATCGATCCTATTGTGGGTGCTGCAGTGATGTTGAAGGGTATGACTGTCCAGTATCTTCTTCGGAGTTGCTTCAAGGTAGAGCCTGGACATACAATTCTTGTGCATGCAGCAGCTGGGGGAGTGGGATCTTTGTTATGCCAATGGGGCAAAGCCCTTGGTGCAACTGTCATTGGCACCGTATCTAATGAGGAGAAGGCAGCACAAGCAGCTGAAGATGGATGCCATCATCCGATTATCTATACCAAGGAAAACTTTGTAGAACGTGTCAAAGAAATTACAAAGGGACAAGGAGTGCATGTTGTCTATGACTCTGTGGGCAAGGACACGTTCCAxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxCACATTCCAGGGGTCCTTGGAGTGCTTGGCAGTCAGAGGTTATATGGTGAGTTTTGGCCAATCATCAGGCATGGTAGATCCTTTTCCTATGTCAGTCCTTGCATCAAAGTCGTTATTTTTAACCCGACCTTCCATGATGCATTACATTGCAACTCGAGATGAGCTATTGAGATGTGCAGGGGAAGTATTTTCTGCTGTTGCAAATGGTATCCTAAAAGTTCGTGTTACCAAGACATACCCTTTGTCAGAAGCTGCAAAGGCTCATGCTGATCTGGAAGCTAGGAAGACAACAGGTTCAATTGTTTTAATCCCGGACAATAGTTGA___TGCATGAAAATGAGGGACGACTGCCGATCGGTCTATGAATGAATGTAGTTGCTTGCATGACATGTTGAGGCACCATACTATTTCTAGTTACTTTAAATTGCTTGAACATCAGTTTAATACTATGTATATAGTAAATACACAGGTAAATGGTTCCATATCTTCTCATATTGTTAATGATATCTGTAATACTTACTAATTATTGAGTCATTTTCATGTTGACAATTTGGAATAAAATCCTAAAACTAATTGCCTATGAGGATTTTATTAGTTATAGGCAAGCGATGAAATTATTTATGAAGAGATTTCGAGGTAACAAGCCTTATTTACCATATTGCAATTTG

Fln Alignment:

Contig16344___MLLKPRFTTPCAWRESDQXXXXXXXXXXXXTNFHPQSKPIRSFKAFASMVKAIRVHELGGAEVLKWEDVEVPEPGAGEILVKHKAIGVNFIDIYYRKGVYKTEKLPFVPGMEAVGVVTAVGPGLTGKNVGDLVAYAGNPMGSYAEEQILPANQVVPFPSTIDPIVGAAVMLKGMTVQYLLRSCFKVEPGHTILVHAAAGGVGSLLCQWGKALGATVIGTVSNEEKAAQAAEDGCHHPIIYTKENFVERVKEITKGQGVHVVYDSVGKDTFQxxxxxxxxxxxxxxxxxxxxxxxxxTFQGSLECLAVRGYMVSFGQSSGMVDPFPMSVLASKSLFLTRPSMMHYIATRDELLRCAGEVFSAVANGILKVRVTKTYPLSEAAKAHADLEARKTTGSIVLIPDNS
C0PSW7_______________MLLNPRFNKLCTWRESDQLSLLSVSLRLVSTNFHPQSKPIRFFKAFATMVKAITVHELGGPEVLKWEDVEVGEPGEGEILVKHKAIGVNFIDVYYRKGVYKAEKLPFIPGREAVGVVTAVGQGLTGRKVGDLVAYAGNPMGSYAEEQILPANQVVPFPSTLDPILGAAVMLKGMTVQYLLRSCFKVEPGHTILVHAAAGGVGSLLCQWGEALGATVIGTVSNEEKAAQAAEDGCHHPIIYTQENFVERVKEITKGQGVHVVYDSVGKDTFQxxxxxxxxxxxxxxxxxxxxxxxxxTFQGSLECLASRGYMVSFGQSSGMVDPVPMSVLAPKSLFLTRPSLMHYTTTRDELLRCAGEVFTAVANGVLKVRVTKTYPLSEAAKAHADLEARKTTGSIVLIPDNS

SNPs (tot: 17)

Position	A %	C %	G %	T %	Probability
199	12	87			0.992554
567	87		12		0.992554
570			75	25	1.0
597			85	14	0.993533
606		14		85	0.993533
607	14			85	0.993533
612	28	71			1.0
615	85		14		0.993533
616		83	16		0.994512
621		83	16		0.994512
623		16		83	0.994512
624	83		16		0.994512
628		80		20	0.995493
629		80	20		0.995493
630	20			80	0.995493
631		80	20		0.995493
636	33			66	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain