SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene15847

Length: 2456 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene15847
AATGTACTCAGTTACCCACGCGTTTCCTTGCTTCGGAGAGGCCTCGATATCGAGCCCCGCAGCAGCAGTAGAAGGGTTTACAAAAAAAGATGGAGACGATGGAGATGCCAGTGCCTGCACATCGCCTGGGCACCGATACCCAGCAGCTCAACATCGATTTTTCCAATGTTGACTTGGATTCAATACAGCTTCCTCCCGGCGAAGATTTTGGCATCAAAAGTGAGGATGAAGAGGAAGAAGAAGATTCACTAGAGTTCGAGACTGGGTTCGGCAACATTGTAGTTGTAGACAATCTCCCGGTTGTCCCTCCAGAGAAGTTTGAGAAACTCGAAGGTGTTGTTCGCAAGATCTTTGGGCAGATTGGTACAATCAGGGAGAGAGGGCTTTGGATGCCGATTCATCCCGAAACCCAGAAGACTCAAGGATATTGCTTTATAGAGTACAATACCTCTCAGGAAGCACAAACAGCAAAAGATCAAACAAATGGATATAAGCTAGACAAATCCCACGTATTTGCTGTGAGCATGTTTGATGATTTTGATAGATACATGAAAGTATCAAACGAATGGATTTCTCCAGAAACGAAGGCCTATACACCTGGGGAAAATTTACAACAGTGGCTAACAGATGAGAAAGGACGTGATCAATTTGTTATCCGTGCGAACACTGACACAGAAGTTTATTGGAATGATCCTAGACATCTCAAACCAGATCTGGTCTACCGCCGTGGATACTGGACTGAAAGCTTTGTCCAGTGGTCATCTCTTGGAACATACCTGGCAACAGTGCACAGGCAGGGAGCAGCTATTTGGGGAGGTGCTACAACCTTTAACCGTCTGATGCGCTTTGCACATCCACAGGTCAAGTTGATAGATTTTTCTCCTGGTGAAAAATTTTTGGTTACTTACAGCAGTCACGAGCCAAGTAATCCTCGAGATACACAGAGGGTTACCCTTAACATTTTTGACGTGAGGACTGGTAAAGTATTGAGAGAGTTTAAAGGAAGTGCTGATGATTTTGCAACTGGCGGAACTGGTGGTGTTGCTGGTGTGTCTTGGCCTGTATTCAGATGGGTTGGTGGCAGAGATGACAAATACTTTGCCCGCATTGGGAAAAATGCCATCAGTGTTTATGAAACAGATACCATGAGTCTCCTAGATAAGAAATCCCTCAAAGTTGACAATGTAATGGATTTCTGTTGGTCACCAACAGATCCAATACTAGCCTTGTTTGTTCCAGAAGGAGGAGGTGGTAACCAACCTGCAAAAGTGAGTCTTGTACAAATCCCTGGAAGGGAAGAACTGCGGCAGAAAAATCTGTTCAGTGTAAGTGATTGCAAAATGTATTGGCAAAGCAATGGGGACTACTTAGCAGTCAAAGTTGACCGCTATACAAAGACGAAGAAAAGTACATACACAGGTTTTGAACTTTTCCGAATAAAGGAAAGAGATATCCCCATAGAGGTTCTGGAATTAGAGAACAAGAATGACAAGATCATTGCATTCGCTTGGGAGCCCAAAGGCCACAGGTTTGCTATTATCCACGGGGATGGACCCAGGCCAGATATTAGTTTTTATTCAATGAGGAGCCCTAATAACCTGGGCCGGGTTTCCAAGCTGGCTACCATTAAAGGAAAACAAGCAAATTCTCTTTACTTGTTGCCCTTTGCTGTGACTGTTTATTATTCTTGCAGGGCTGAAAACCTTAAATGGTCTCCTTGAATTCTACAATGTCGATGAACTTGAGACAATGGCAACTGGTGAACATTTTATGGCAACTGATATTGAGTGGGATCCTACTGGAAGGTATGCTGCAACAGCAGTCACAACTGTTCATGAAATGGAAAATGGTTTTAACATATGGTCTTTCAATGGAAAGCTGTTGTACCGCATTCCCAAAGATCATTTCTATCAGCTATTATGGCGTCCAAGGCCAGCCTCTCTTTTAAGCCCAGAGAAAGAGGAGGAGATTTCTAAGAATTTGAGAAAGTATAGCAAGAAGTATGAGGCTGAAGACCAGGATGTTTCATTGTTGTTGAGTGAGCAAGACAGGGAGAAACGGAAGAGATTGCAAGAGGAGTGGCAGGGCTGGGTCAATGAATGGAAACGTTTTCACGAGGAAGAGAAATTATATAGGCAGGAACTACGTGATGGAGAGCCTAGTGATGAAGAAGAGGAATATGAAGCAGAGGAAGTTGAAGTTGAAGAGTTAGTAGATGTTACTGAAGAGATTGCTTCATTTGGTTTTGAGCAGATGGACTGAGTGATTGTATAGCATGAATCTATTTGTGCCTTCATGCAGGGAGATAGCTTGGGCAGAAAATGCAGTGGTTTTCAACTGTTTTCATGTCACACTCACAGTAGCATAGATACTTGTTTGTTGAAAGTTCTGTAGTATGGGCTTTGAAATGCCCAATTTCTCAAAAGAAAATTGCAGTTCGACAGTTTGATCTGTTA

Ace file of the UniGene sp_v2.0_unigene15847 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	translation initiation factor 3 subunit B [Arabidopsis thaliana] sp\|Q9C5Z1.1\|EIF3B_ARATH RecName: Full=Eukaryotic translation initiation factor 3 subunit B; Short=eIF3b; AltName: Full=eIF-3-eta; AltName: Full=eIF3 p110; AltName: Full=p82 gb\|AAG53615.1\|AF2	-	-	68%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	95%
Blast2go	eukaryotic translation initiation factor 3 subunit b-like	-	-	87%

Source	GOs	Term	Type	Identity
Blast2go	nucleic acid binding	GO:0003676	Molecular Function	87%
Blast2go	protein binding	GO:0005515	Molecular Function	87%
Blast2go	nucleus	GO:0005634	Cellular Component	87%
Blast2go	cytosol	GO:0005829	Cellular Component	87%

Source	InterPros	Term	Type	Identity
Sma3	RNA recognition motif domain	IPR000504	-	-
Sma3	Six-bladed beta-propeller, TolB-like	IPR011042	-	-
Sma3	Translation initiation factor eIF-3b	IPR011400	-	-
Sma3	Nucleotide-binding, alpha-beta plait	IPR012677	-	-
Sma3	Translation initiation factor 2A, beta propellor-like domain	IPR013979	-	-
Sma3	WD40/YVTN repeat-like-containing domain	IPR015943	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NWV8

Fln msg: Separated hits, possible frame ERROR between 1664 and 1678,

Fln protein: METMEMPVPAHRLGTDTQQLNIDFSNVDLDSIQLPPGEDFGIKSEDEEEEEDSLEFETGFGNIVVVDNLPVVPPEKFEKLEGVVRKIFGQIGTIRERGLWMPIHPETQKTQGYCFIEYNTSQEAQTAKDQTNGYKLDKSHVFAVSMFDDFDRYMKVSNEWISPETKAYTPGENLQQWLTDEKGRDQFVIRANTDTEVYWNDPRHLKPDLVYRRGYWTESFVQWSSLGTYLATVHRQGAAIWGGATTFNRLMRFAHPQVKLIDFSPGEKFLVTYSSHEPSNPRDTQRVTLNIFDVRTGKVLREFKGSADDFATGGTGGVAGVSWPVFRWVGGRDDKYFARIGKNAISVYETDTMSLLDKKSLKVDNVMDFCWSPTDPILALFVPEGGGGNQPAKVSLVQIPGREELRQKNLFSVSDCKMYWQSNGDYLAVKVDRYTKTKKSTYTGFELFRIKERDIPIEVLELENKNDKIIAFAWEPKGHRFAIIHGDGPRPDISFYSMRSPNNLGRVSKLATIKGKQANSLYLLxxxxxxFIILAGLKTLNGLLEFYNVDELETMATGEHFMATDIEWDPTGRYAATAVTTVHEMENGFNIWSFNGKLLYRIPKDHFYQLLWRPRPASLLSPEKEEEISKNLRKYSKKYEAEDQDVSLLLSEQDREKRKRLQEEWQGWVNEWKRFHEEEKLYRQELRDGEPSDEEEEYEAEEVEVEELVDVTEEIASFGFEQMD*

Protein Length: 726

Fln nts: AATGTACTCAGTTACCCACGCGTTTCCTTGCTTCGGAGAGGCCTCGATATCGAGCCCCGCAGCAGCAGTAGAAGGGTTTACAAAAAAAG_-_ATGGAGACGATGGAGATGCCAGTGCCTGCACATCGCCTGGGCACCGATACCCAGCAGCTCAACATCGATTTTTCCAATGTTGACTTGGATTCAATACAGCTTCCTCCCGGCGAAGATTTTGGCATCAAAAGTGAGGATGAAGAGGAAGAAGAAGATTCACTAGAGTTCGAGACTGGGTTCGGCAACATTGTAGTTGTAGACAATCTCCCGGTTGTCCCTCCAGAGAAGTTTGAGAAACTCGAAGGTGTTGTTCGCAAGATCTTTGGGCAGATTGGTACAATCAGGGAGAGAGGGCTTTGGATGCCGATTCATCCCGAAACCCAGAAGACTCAAGGATATTGCTTTATAGAGTACAATACCTCTCAGGAAGCACAAACAGCAAAAGATCAAACAAATGGATATAAGCTAGACAAATCCCACGTATTTGCTGTGAGCATGTTTGATGATTTTGATAGATACATGAAAGTATCAAACGAATGGATTTCTCCAGAAACGAAGGCCTATACACCTGGGGAAAATTTACAACAGTGGCTAACAGATGAGAAAGGACGTGATCAATTTGTTATCCGTGCGAACACTGACACAGAAGTTTATTGGAATGATCCTAGACATCTCAAACCAGATCTGGTCTACCGCCGTGGATACTGGACTGAAAGCTTTGTCCAGTGGTCATCTCTTGGAACATACCTGGCAACAGTGCACAGGCAGGGAGCAGCTATTTGGGGAGGTGCTACAACCTTTAACCGTCTGATGCGCTTTGCACATCCACAGGTCAAGTTGATAGATTTTTCTCCTGGTGAAAAATTTTTGGTTACTTACAGCAGTCACGAGCCAAGTAATCCTCGAGATACACAGAGGGTTACCCTTAACATTTTTGACGTGAGGACTGGTAAAGTATTGAGAGAGTTTAAAGGAAGTGCTGATGATTTTGCAACTGGCGGAACTGGTGGTGTTGCTGGTGTGTCTTGGCCTGTATTCAGATGGGTTGGTGGCAGAGATGACAAATACTTTGCCCGCATTGGGAAAAATGCCATCAGTGTTTATGAAACAGATACCATGAGTCTCCTAGATAAGAAATCCCTCAAAGTTGACAATGTAATGGATTTCTGTTGGTCACCAACAGATCCAATACTAGCCTTGTTTGTTCCAGAAGGAGGAGGTGGTAACCAACCTGCAAAAGTGAGTCTTGTACAAATCCCTGGAAGGGAAGAACTGCGGCAGAAAAATCTGTTCAGTGTAAGTGATTGCAAAATGTATTGGCAAAGCAATGGGGACTACTTAGCAGTCAAAGTTGACCGCTATACAAAGACGAAGAAAAGTACATACACAGGTTTTGAACTTTTCCGAATAAAGGAAAGAGATATCCCCATAGAGGTTCTGGAATTAGAGAACAAGAATGACAAGATCATTGCATTCGCTTGGGAGCCCAAAGGCCACAGGTTTGCTATTATCCACGGGGATGGACCCAGGCCAGATATTAGTTTTTATTCAATGAGGAGCCCTAATAACCTGGGCCGGGTTTCCAAGCTGGCTACCATTAAAGGAAAACAAGCAAATTCTCTTTACTTGTTGCCxxxxxxxxxxxxxxxGTTTATTATTCTTGCAGGGCTGAAAACCTTAAATGGTCTCCTTGAATTCTACAATGTCGATGAACTTGAGACAATGGCAACTGGTGAACATTTTATGGCAACTGATATTGAGTGGGATCCTACTGGAAGGTATGCTGCAACAGCAGTCACAACTGTTCATGAAATGGAAAATGGTTTTAACATATGGTCTTTCAATGGAAAGCTGTTGTACCGCATTCCCAAAGATCATTTCTATCAGCTATTATGGCGTCCAAGGCCAGCCTCTCTTTTAAGCCCAGAGAAAGAGGAGGAGATTTCTAAGAATTTGAGAAAGTATAGCAAGAAGTATGAGGCTGAAGACCAGGATGTTTCATTGTTGTTGAGTGAGCAAGACAGGGAGAAACGGAAGAGATTGCAAGAGGAGTGGCAGGGCTGGGTCAATGAATGGAAACGTTTTCACGAGGAAGAGAAATTATATAGGCAGGAACTACGTGATGGAGAGCCTAGTGATGAAGAAGAGGAATATGAAGCAGAGGAAGTTGAAGTTGAAGAGTTAGTAGATGTTACTGAAGAGATTGCTTCATTTGGTTTTGAGCAGATGGACTGA___GTGATTGTATAGCATGAATCTATTTGTGCCTTCATGCAGGGAGATAGCTTGGGCAGAAAATGCAGTGGTTTTCAACTGTTTTCATGTCACACTCACAGTAGCATAGATACTTGTTTGTTGAAAGTTCTGTAGTATGGGCTTTGAAATGCCCAATTTCTCAAAAGAAAATTGCAGTTCGACAGTTTGATCTGTTA

Fln Alignment:

Contig15847___METMEMP--VPAHRLGTDTQQLNIDFSNVDLDSIQLPPGEDFGIKXXXXXXXXXXXXXETGFGNIVVVDNLPVVPPEKFEKLEGVVRKIFGQIGTIRERGLWMPIHPETQKTQGYCFIEYNTSQEAQTAKDQTNGYKLDKSHVFAVSMFDDFDRYMKVSNEWISPETKAYTPGENLQQWLTDEKGRDQFVIRANTDTEVYWNDPRHLKPDLVYRRGYWTESFVQWSSLGTYLATVHRQGAAIWGGATTFNRLMRFAHPQVKLIDFSPGEKFLVTYSSHEPSNPRDTQRVTLNIFDVRTGKVLREFKGSADDFXXXXXXXXXXVSWPVFRWVGGRDDKYFARIGKNAISVYETDTMSLLDKKSLKVDNVMDFCWSPTDPILALFVPEGGGGNQPAKVSLVQIPGREELRQKNLFSVSDCKMYWQSNGDYLAVKVDRYTKTKKSTYTGFELFRIKERDIPIEVLELENKNDKIIAFAWEPKGHRFAIIHGDGPRPDISFYSMRSPNNLGRVSKLATIKGKQANSLYLLPxxxxxFIILAGLKTLNGLLEFYNVDELETMATGEHFMATDIEWDPTGRYAATAVTTVHEMENGFNIWSFNGKLLYRIPKDHFYQLLWRPRPASLLSPEKEEEISKNLRKYSKKYEAEDQDVSLLLSEQDREKRKRLQEEWQGWVNEWKRFHEEEKLYRQELRDGEPSDXXXXXXXXXXXXXXXXXXXXEIASFGFEQMD
A9NWV8_______________METIEMPLPVPVHRLGNDIQQLNIDFSNVDLDSIQLPPGEDFGIKSEDEEEEEDSLEFETGFGNIVVVDNLPVVPPEKFEKLEGVVRKIFGQIGTIRERGLWMPIHPETQKTQGYCFIEYNTPQEAQTAKDQTNGYKLDKSHVFAVNMFDDFDKYMKVPNEWIPPETKAYTPGENLQQWLTDEKGRDQYVIRSNTDTEVYWNDPRQLKPELVYRRGYWTESFVQWSSLGTYLATVHRQGAAIWGGGTTFNRLMRFAHPQVKLIDFSPGENFLVTYSSHEPSNPRDTQRVTLNIFEVRTGKVLREFKGSADDFATGGTGGVAGVSWPVFRWAGGRDDKYFARIGKNAISVYETDTMTLLDKKSLKVDNVMDFSWSPTDPILALFVPEGGGGNQPAKVSLVQIPGREELRQKNLFSVSDCKMYWQSNGDYLAVKVDRYTKTKKSTYTGFELFRIKERDIPIEVLELENKNDKIIAFAWEPKGHRFAIIHGDGPRPDVSFYSMRSPNNVGRVSKLATIKGKQANSLYWSPxxxxxFIILAGLKALNGQLEFFNVDELETMATGEHFMATDIEWDPTGRYAATSVTVVHEMENGFNIWSFNGKLLYRIPRDHFYQLLWRPRPPSLLSPEKEEEISKNLRKYSKKYEAEDQDVSLLLSEQDREKRKRLQEDWQGWVNEWKRLHEEEKLFRQDLRDGEPSDEEEEYEAEEVEVEELIDVTEEIASFGFEQLD

SNPs (tot: 65)

Position	A %	C %	G %	T %	Probability
332	12			87	0.999964
365		78		14	0.999974
366		78	14		0.999974
372	21	78			1.0
373	21			78	1.0
375		78		21	1.0
379			21	78	1.0
380	10	82	6		1.0
381		93	6		0.999453
382			10	89	1.0
384	7			92	0.999664
385		7		92	0.999664
388	88		7		0.999079
427	91			8	0.999807
428	91		8		0.999807
429	8			91	0.999807
431	91			8	0.999807
432	91		8		0.999807
436			8	91	0.999807
439	8			91	0.999807
444	8			91	0.999807
445		8		91	0.999807
456		89	10		0.999919
458		5	11	83	0.999887
461	11			88	0.999937
464		11		88	0.999952
469			12	87	0.999964
470			12	87	0.999964
473	13			86	0.999973
475	86		13		0.999973
477	86	13			0.999973
478			13	86	0.999973
504	10			90	0.90298
505	12		87		0.992554
509		14		85	0.993533
510		14		85	0.993533
512	85		14		0.993533
514	85	14			0.993533
521		83	16		0.994512
571		14		85	0.993533
572	85	14			0.993533
575	85			14	0.993533
606	16		83		0.994512
636		16		83	0.994512
650	85		14		0.993533
708		20		80	0.995493
709		20	80		0.995493
710	20			80	0.995493
711	20	80			0.995493
713	20	80			0.995493
792	33			66	0.997452
795			66	33	0.997452
796		33	66		0.997452
798	66	33			0.997452
799		66	33		0.997452
800	33	66			0.997452
1113	66		33		0.997452
1193	14		85		0.993533
1220	12		87		0.992554
1221		12	87		0.992554
1223	87			12	0.992554
1224		87		12	0.992554
1459	33		66		0.997452
1480	33		66		0.997452
1489			33	66	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain