SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene15344

Length: 2473 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene15344
CGTCGTGACCTTGGCTGTCACTCAGAGTCGATGTTATCCAGGACGGCAGTCTAGATGCGTTGGGAAGAGGGAACATTTGGACGTTAGTGCCCTCAAAATGCGATATTTTGCGGAATCAATTCTCACTTTGTTTCGACCAATGTGACCACGCCATTCCTGAGGTCTGTGTCCTTTGCTGCCACTCTAGCTTTTATTGGATTTGGCTTTAGCTCCCACGATATTCACAGTGTCGACGATGATGGATAGGCCGGCCATGGCTCTGTGTGTGCTTTCAATTCTCATATCATGCAGCTTTGTATTGGTAAGAAGCGCATCGGATCACAGTTATAAAGTGGGAGACGAGGTGCCCCTGTATGTCAACAAGGTCGGCCCCTTTCAGAACCCCAGTGAGACATATCGTTATTTTGATTTGCCATTCTGTCCTCCAGATCACGTGACTGAAAAGAAGGAGGCTCTTGGAGAAGTCTTAAATGGTGATCGTATGGTTGATGCTAGGTATCGGTTAAAATTCCGGGTGGACAAAGAGACCAACTCCCTTTGCAAGAAGAAGCTTTCAAAAGAGGATCTAGCAAAGTTCAGGGATGCTGTTAAGAATGATTACTACTTTCAGATGTACTATGATGATCTGCCTATATGGGGCTTTGTTGGAAAGGTTGAAAATGAAAATAAAGGCGATGAGAAGAAGCCTAGGCATTTGCTTTACAGACATGTGCACTTTGATGTCCTATATAACGATGACCGAGTCATAGAAATCAGTGTGCATACAGATCCTCAGTACGCTGTGGATATTACCGAGGACAAAGAGATTGAGGTTGAGTTCATGTACTCTGTTAAGTGGAAAAAAACTGACATCCTCTTTGAACGCCGAATGGATAAATATGCGAAATCTTCATTTTTGCCACAGCACTTGGAAATTCATTGGTTCTCCATTATCAATTCGTGTGTAACTGTTCTTCTCCTGACGGGATTCCTGGCCACAATTCTCATGCGCATTCTGAAGAATGATTTTATCAAATACTCTCGTGATGAGGAGTCAATGGATGATCAAGAGGAGACAGGATGGAAGTACATTCATGGAGATGTTTTTCGATATCCTTCACATAAGTCTCTATTTTCTGCTGTTCTTGGCTCTGGAACTCAGCTGTTAGTACTTACTATCTTTATCTTTGTGCTTGCATTGGTGGGTGTTTTCTATCCATACAACAGGGGAGCCCTATATACTGCTCTTGTTGTTATATATGCTCTTACATCTGGCATAGCAGGCTACGTTGCAGCTTCCTTTTATGGTCAACTTGAAGGACGAAATTGGGTAAGGAACCTTTTGCTTACAGGTTGCCTCTTTTGTGGGCCGCTATTCTTAACCTTTTCCTTCCTGAACACAGTTGCAATAGCATACAATGCCACAGCAGCCTTGCCATTTGGTACTATTGTTGTGATTGTTCTCATCTGGACACTAATCACCTTTCCTTTGCTCGTGTTGGGCGGCATAGCTGGTAAGAACAGCAAGGCAGAGTTTCAAGCTCCATGCCGTACCACAAAGTATCCCCGGGAGATTCCACCATTACCATGGTACAGGAGGACCGTTCCTCAAATGGCAATGGCAGGCTTTTTGCCATTTAGTGCCATTTATATTGAGCTATATTACATTTTTGCAAGTATCTGGGGTCACAAGATTTATACAATATATAGCATTCTCTTCATTGTCTTCATCATCCTCATCATTGTTACCTCGTTTATTACAATTGCATTAACATACTTTCAGCTTGCAGCAGAGGATCATGAATGGTGGTGGAGGTCTGTTTTATGTGGAGGATCAACTGGTTTGTTCGTGTTTGGATACTGTTTCTATTATTACTTTGCACGATCTGATATGACTGGCTTCATGCAAGTGTCATTCTTCTTTGGATACATGGCTTGCATATGTTATGGCTTCTTCCTAATGCTGGGAACAGTTGGATTCCAGTCTTCTTTACTGTTTGTTCGCCATATATACCGTTCCATTAAGTGTGAGTAAAACTCTTCCAGTGCAGAGCACGCAGATTTTGGTCTATCTGTCCATCCTGTAGATTAGTTGCAGATTATAATGGTCCTGGCTAGCATACTCCTTTGGACATGCTTGTCCAAAATGGAGTTGGGCGGTAAGCTCTTACCGTTAGGTGAATGCTTACATGTGGTAAGTTTCTAGGAAATAAGGTGAATAATGACACTGGCAGATTAGGCTGAGGTGAACAATTGAAGAACTAGTATATAGTTATGTTGATGAATAGTTACGGAGGCATGTTTTATCTCGAACAGCTTTGTTTGGTGATACCTATTGTCAGGCATAAGCCAATTTTTTTAGGTCTCAAATCTATGGAGTAAGATGAATAAGCATCGAGCAAGCTTCAGGGATCTTCAAATGTTACCTTTCTGTTGGCCTGTATTTTATATTCCTGGTCTTTTCTTTGGTAAGAATAAATGTTTGTGTTTCAAT

Ace file of the UniGene sp_v2.0_unigene15344 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	putative endomembrane protein 70 [Arabidopsis thaliana] gb\|AAK96649.1\| T5E21.14/T5E21.14 [Arabidopsis thaliana] gb\|AAM16222.1\| At1g14670/T5E21.14 [Arabidopsis thaliana] gb\|AEE29200.1\| putative endomembrane protein 70 [Arabidopsis thaliana]	-	-	73%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	84%
Blast2go	transmembrane 9 superfamily protein member 1 precursor	-	-	87%

Source	GOs	Term	Type	e value	Identity
Blast2go	membrane	GO:0016020	Cellular Component	0.0	87%
Blast2go	Golgi apparatus	GO:0005794	Cellular Component	0.0	87%

Source	InterPros	Term	Type	Identity
Sma3	Aldo/keto reductase	IPR001395	-	-
Sma3	Tetratricopeptide TPR-1	IPR001440	-	-
Sma3	Ribosomal protein L1	IPR002143	-	-
Sma3	Nonaspanin (TM9SF)	IPR004240	-	-
Sma3	Carbamoyl-phosphate synthetase, large subunit, ATP-binding	IPR005479	-	-
Sma3	Ribosomal protein L13	IPR005822	-	-
Sma3	Ribosomal protein L13, bacterial-type	IPR005823	-	-
Sma3	Tetratricopeptide-like helical	IPR011990	-	-
Sma3	Tetratricopeptide repeat-containing domain	IPR013026	-	-
Sma3	Ribosomal protein L1, 2-layer alpha/beta-sandwich	IPR016094	-	-
Sma3	Ribosomal protein L1, 3-layer alpha/beta-sandwich	IPR016095	-	-
Sma3	Aldo/keto reductase, conserved site	IPR018170	-	-
Sma3	Tetratricopeptide repeat	IPR019734	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LKM1

Fln msg:

Fln protein: MMDRPAMALCVLSILISCSFVLVRSASDHSYKVGDEVPLYVNKVGPFQNPSETYRYFDLPFCPPDHVTEKKEALGEVLNGDRMVDARYRLKFRVDKETNSLCKKKLSKEDLAKFRDAVKNDYYFQMYYDDLPIWGFVGKVENENKGDEKKPRHLLYRHVHFDVLYNDDRVIEISVHTDPQYAVDITEDKEIEVEFMYSVKWKKTDILFERRMDKYAKSSFLPQHLEIHWFSIINSCVTVLLLTGFLATILMRILKNDFIKYSRDEESMDDQEETGWKYIHGDVFRYPSHKSLFSAVLGSGTQLLVLTIFIFVLALVGVFYPYNRGALYTALVVIYALTSGIAGYVAASFYGQLEGRNWVRNLLLTGCLFCGPLFLTFSFLNTVAIAYNATAALPFGTIVVIVLIWTLITFPLLVLGGIAGKNSKAEFQAPCRTTKYPREIPPLPWYRRTVPQMAMAGFLPFSAIYIELYYIFASIWGHKIYTIYSILFIVFIILIIVTSFITIALTYFQLAAEDHEWWWRSVLCGGSTGLFVFGYCFYYYFARSDMTGFMQVSFFFGYMACICYGFFLMLGTVGFQSSLLFVRHIYRSIKCE*

Protein Length: 594

Fln nts: CGTCGTGACCTTGGCTGTCACTCAGAGTCGATGTTATCCAGGACGGCAGTCTAGATGCGTTGGGAAGAGGGAACATTTGGACGTTAGTGCCCTCAAAATGCGATATTTTGCGGAATCAATTCTCACTTTGTTTCGACCAATGTGACCACGCCATTCCTGAGGTCTGTGTCCTTTGCTGCCACTCTAGCTTTTATTGGATTTGGCTTTAGCTCCCACGATATTCACAGTGTCGACG_-_ATGATGGATAGGCCGGCCATGGCTCTGTGTGTGCTTTCAATTCTCATATCATGCAGCTTTGTATTGGTAAGAAGCGCATCGGATCACAGTTATAAAGTGGGAGACGAGGTGCCCCTGTATGTCAACAAGGTCGGCCCCTTTCAGAACCCCAGTGAGACATATCGTTATTTTGATTTGCCATTCTGTCCTCCAGATCACGTGACTGAAAAGAAGGAGGCTCTTGGAGAAGTCTTAAATGGTGATCGTATGGTTGATGCTAGGTATCGGTTAAAATTCCGGGTGGACAAAGAGACCAACTCCCTTTGCAAGAAGAAGCTTTCAAAAGAGGATCTAGCAAAGTTCAGGGATGCTGTTAAGAATGATTACTACTTTCAGATGTACTATGATGATCTGCCTATATGGGGCTTTGTTGGAAAGGTTGAAAATGAAAATAAAGGCGATGAGAAGAAGCCTAGGCATTTGCTTTACAGACATGTGCACTTTGATGTCCTATATAACGATGACCGAGTCATAGAAATCAGTGTGCATACAGATCCTCAGTACGCTGTGGATATTACCGAGGACAAAGAGATTGAGGTTGAGTTCATGTACTCTGTTAAGTGGAAAAAAACTGACATCCTCTTTGAACGCCGAATGGATAAATATGCGAAATCTTCATTTTTGCCACAGCACTTGGAAATTCATTGGTTCTCCATTATCAATTCGTGTGTAACTGTTCTTCTCCTGACGGGATTCCTGGCCACAATTCTCATGCGCATTCTGAAGAATGATTTTATCAAATACTCTCGTGATGAGGAGTCAATGGATGATCAAGAGGAGACAGGATGGAAGTACATTCATGGAGATGTTTTTCGATATCCTTCACATAAGTCTCTATTTTCTGCTGTTCTTGGCTCTGGAACTCAGCTGTTAGTACTTACTATCTTTATCTTTGTGCTTGCATTGGTGGGTGTTTTCTATCCATACAACAGGGGAGCCCTATATACTGCTCTTGTTGTTATATATGCTCTTACATCTGGCATAGCAGGCTACGTTGCAGCTTCCTTTTATGGTCAACTTGAAGGACGAAATTGGGTAAGGAACCTTTTGCTTACAGGTTGCCTCTTTTGTGGGCCGCTATTCTTAACCTTTTCCTTCCTGAACACAGTTGCAATAGCATACAATGCCACAGCAGCCTTGCCATTTGGTACTATTGTTGTGATTGTTCTCATCTGGACACTAATCACCTTTCCTTTGCTCGTGTTGGGCGGCATAGCTGGTAAGAACAGCAAGGCAGAGTTTCAAGCTCCATGCCGTACCACAAAGTATCCCCGGGAGATTCCACCATTACCATGGTACAGGAGGACCGTTCCTCAAATGGCAATGGCAGGCTTTTTGCCATTTAGTGCCATTTATATTGAGCTATATTACATTTTTGCAAGTATCTGGGGTCACAAGATTTATACAATATATAGCATTCTCTTCATTGTCTTCATCATCCTCATCATTGTTACCTCGTTTATTACAATTGCATTAACATACTTTCAGCTTGCAGCAGAGGATCATGAATGGTGGTGGAGGTCTGTTTTATGTGGAGGATCAACTGGTTTGTTCGTGTTTGGATACTGTTTCTATTATTACTTTGCACGATCTGATATGACTGGCTTCATGCAAGTGTCATTCTTCTTTGGATACATGGCTTGCATATGTTATGGCTTCTTCCTAATGCTGGGAACAGTTGGATTCCAGTCTTCTTTACTGTTTGTTCGCCATATATACCGTTCCATTAAGTGTGAGTAA___AACTCTTCCAGTGCAGAGCACGCAGATTTTGGTCTATCTGTCCATCCTGTAGATTAGTTGCAGATTATAATGGTCCTGGCTAGCATACTCCTTTGGACATGCTTGTCCAAAATGGAGTTGGGCGGTAAGCTCTTACCGTTAGGTGAATGCTTACATGTGGTAAGTTTCTAGGAAATAAGGTGAATAATGACACTGGCAGATTAGGCTGAGGTGAACAATTGAAGAACTAGTATATAGTTATGTTGATGAATAGTTACGGAGGCATGTTTTATCTCGAACAGCTTTGTTTGGTGATACCTATTGTCAGGCATAAGCCAATTTTTTTAGGTCTCAAATCTATGGAGTAAGATGAATAAGCATCGAGCAAGCTTCAGGGATCTTCAAATGTTACCTTTCTGTTGGCCTGTATTTTATATTCCTGGTCTTTTCTTTGGTAAGAATAAATGTTTGTGTTTCAAT

Fln Alignment:

Contig15344___SDHSYKVGDEVPLYVNKVGPFQNPSETYRYFDLPFCPPDHVTEKKEALGEVLNGDRMVDARYRLKFRVDKETNSLCKKKLSKEDLAKFRDAVKNDYYFQMYYDDLPIWGFVGKVENENKGDEKKPRHLLYRHVHFDVLYNDDRVIEISVHTDPQYAVDITEDKEIEVEFMYSVKWKKTDILFERRMDKYAKSSFLPQHLEIHWFSIINSCVTVLLLTGFLATILMRILKNDFIKYSRDEESMDDQEETGWKYIHGDVFRYPSHKSLFSAVLGSGTQLLVLTIFIFVLALVGVFYPYNRGALYTALVVIYALTSGIAGYVAASFYGQLEGRNWVRNLLLTGCLFCGPLFLTFSFLNTVAIAYNATAALPFGXXXXXXXXXXXXTFPLLVLGGIAGKNSKAEFQAPCRTTKYPREIPPLPWYRRTVPQMAMAGFLPFSAIYIELYYIFASIWGHKIYTIYXXXXXXXXXXXXXXXXXXIALTYFQLAAEDHEWWWRSVLCGGSTGLFVFGYCFYYYFARSDMTGFMQVSFFFGYMACICYGFFLMLGTVGFQSSLLFVRHIYRSIKCE
B8LKM1_______________SDHKYSKGDHVPLYANKVGPFQNPSETYRYFDLPFCQPEHMTEKREDLGEVLNGDRMVDAQYKLDFNVDKESEVLCKKKFKAEDVAKFREAVKNSYYFQMYYDDLPLWGFIGKVEKESKIDQK---YSLFTHLHFDIKFNKDRVIEISVRTDPSFTVDITEDKEVEIEFVYSVKWAETDVPFKRRMDVYSKSSSLPQHLEIHWFSIINSCVTVLLLTGFLATILMRVLKNDFIKYSHDEETADEQEETGWKYIHGDVFRYPPHKSLFSAVLGSGTQLLALAIFIFMLALVGVFYPYNRGALYTALVVIYALTSGIAGYTAASFYRQLEGTNWVRNLLLTGCLFCGPLFLTFCFLNTVAIAYNATAALPFGTILVILLIWTLVTSPLLVLGGIAGKNSRAEFQAPCRTTKYPREIPPLPWYRGTVPQMAMAGFLPFSAIYIELYYIFASLWGHKIYTIYSILFIVFIILIIVTAFITIALTYFQLAVEDHQWWWRSVLCGGSTGVFIFGYCVYYYFARSDMTGFMQTSFFFGYMTCICYGFFLMLGTVGFRASLLFVRHIYRSIKCE

SNPs (tot: 86)

Position	A %	C %	G %	T %	Probability
440		75		25	1.0
514	8			92	0.999718
573	90	9			0.999872
574	90	9			0.999872
576			90	9	0.999872
633	94			5	0.999993
634		94	5		0.999993
637		5		94	0.999993
639			5	94	0.999994
642	96	3			0.988939
644	94		5		0.999994
652	94		5		0.999995
661		3	96		0.993591
663		1		94	0.964041
665	4	96			0.99411
666			6	94	0.999998
672		2	4	93	0.970804
673	95		4		0.994598
674	95			4	0.994598
675	95	4			0.994598
678	91			4	0.908347
682		85	8	6	1.0
683			4	95	0.995482
684	4	91			0.919342
685		91	8		1.0
686	93			4	0.979066
687	4	89			0.999986
693			9	90	1.0
697		11		88	1.0
702		90	9		1.0
713			94	5	0.998744
745			93	6	0.999153
746	93			6	0.999153
837		9	4	85	0.999726
1137		7	92		0.999664
1290	93			6	0.999453
1308	92		7		0.999532
1309	92		7		0.999602
1328		92	8		0.999718
1330	84	4	8	4	0.998474
1336	4	17		78	1.0
1337	8		91		0.999807
1338	91	8			0.999807
1340			9	90	0.999872
1343	9	85	4		0.999726
1344	85	9	4		0.999726
1358	10	78		5	0.999769
1512		87		12	0.992554
1517			90	10	0.90298
1788	57		36		1.0
1995	11		88		0.999952
2054			11	88	0.999952
2257	86		4	8	0.999539
2258	4		8	86	0.999539
2259	8	91			0.999807
2263	8	4	86		0.999539
2264	4	8	86		0.999539
2268		8		91	0.999807
2271	86	4	8		0.999539
2273	4	86		9	0.999642
2276	90	9			0.999872
2277	90		9		0.999872
2278	10			90	0.999898
2280		90	10		0.999898
2297	64		35		1.0
2302	5	58			1.0
2308	35	58		5	1.0
2309	58			41	1.0
2316		35	64		1.0
2318		85	14		0.993533
2320		14	85		0.993533
2321	14		71		0.984337
2322	71	14			0.984337
2325			85	14	0.993533
2326		16	83		0.994512
2328	16		83		0.994512
2329	16			83	0.994512
2336	83		16		0.994512
2337	16			83	0.994512
2338		16		83	0.994512
2343		20	80		0.995493
2344	80	20			0.995493
2345	80	20			0.995493
2347		80		20	0.995493
2350	50		50		1.0
2453		33		66	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain