SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene15166

Length: 1113 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene15166

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	cullin 3B [Arabidopsis thaliana] sp\|Q9C9L0.1\|CUL3B_ARATH RecName: Full=Cullin-3B; Short=AtCUL3b gb\|AAG52544.1\|AC013289_11 putative cullin; 66460-68733 [Arabidopsis thaliana] gb\|AEE34961.1\| cullin 3B [Arabidopsis thaliana]	-	-	75%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	81%
Blast2go	protein	-	-	88%

Source	GOs	Term	Type	Identity
Blast2go	endosperm development	GO:0009960	Biological Process	88%
Blast2go	embryo development ending in seed dormancy	GO:0009793	Biological Process	88%
Blast2go	protein binding	GO:0005515	Molecular Function	88%
Blast2go	SCF ubiquitin ligase complex	GO:0019005	Cellular Component	88%

Source	InterPros	Term	Type	Identity
Sma3	Cullin, N-terminal	IPR001373	-	-
Sma3	Small GTPase superfamily	IPR001806	-	-
Sma3	Winged helix-turn-helix transcription repressor DNA-binding	IPR011991	-	-
Sma3		IPR013753	-	-
Sma3	Cullin, conserved site	IPR016157	-	-
Sma3	Cullin homology	IPR016158	-	-

Full-Lengther Next Prediction

Fln status: Putative N-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LNS8

Fln msg: Distance to subject end: 432 aas, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 283 - 735

Fln protein: MDPKYAEKTRKIIEHAIHEIYNHNASGLSFEELYRNAYNMVLHKYGEKLYSGLVTTMTVHLKEIATVIEASQGGLFLEELNRQWMDHNKALQMIRDILMYMDRTFVTNSNKTPVNELGLNLWRDHIVKLPNIRDRLLNTLLDLVYTERTGEIIDRSLMRNVIKMLMDLGPSVYHDDFEKSFLDVTANFYRIESQQFIECCDCGDYMKKAERRLNEEMDRVSNYLDPKSEIKITSVVEREMIANHMTRLVHMENSGLVRMLVCDKYEDLGRMHSLFRRVPIGLQ

Protein Length: 284

Fln nts: ATGGGGACATCAGAGTAATCTGTTGGGTGCGGGCACCACGTCGTTGGGAATTATCAGCGTAGCAGAGCTGCCACATTCCAGATCTATTGGATAAAGCAGTTTTCATTGGGTCAGCAGTGATAAATTTTGTTTCATAACGGACGCAAACCGCATATTATTTTCGAGTAGGAAAAAGGGCTTGAAGGAGGAAAATAAAGGAGGAAAACATGAGTAATACAAGGAGAAGAAATTTTAAATTGAACCATTCAAGCACAAGGTGGAG_-_ATGGATCCAAAGTATGCCGAGAAGACACGGAAGATTATAGAGCATGCGATACATGAGATTTATAATCACAATGCCAGTGGTTTGAGTTTTGAAGAGTTGTACAGGAATGCATACAATATGGTGCTTCACAAGTATGGGGAGAAACTTTATTCAGGTCTGGTGACGACCATGACAGTGCATTTAAAGGAAATAGCGACAGTGATTGAAGCTTCTCAGGGGGGTTTGTTCCTAGAGGAGTTGAATAGGCAATGGATGGACCATAACAAAGCACTACAGATGATTCGAGACATCCTGATGTATATGGATAGAACATTTGTCACCAATTCCAACAAGACCCCAGTCAATGAACTTGGACTGAATTTGTGGAGAGATCACATTGTCAAGTTACCAAATATTCGTGATAGGCTGCTAAATACCCTTCTTGACCTTGTATACACAGAGCGGACGGGTGAAATCATTGATCGAAGTTTAATGCGTAATGTCATTAAAATGTTGATGGATCTGGGTCCTTCTGTTTACCATGATGATTTTGAAAAGTCCTTTTTGGATGTTACGGCCAATTTTTACAGAATAGAGTCCCAGCAGTTTATTGAATGTTGTGATTGTGGTGATTATATGAAGAAAGCAGAAAGAAGGCTAAATGAAGAGATGGACAGAGTTTCAAATTACTTAGATCCAAAGAGCGAGATAAAGATTACCTCTGTTGTTGAGAGGGAGATGATCGCTAACCACATGACGCGGCTGGTCCACATGGAGAACTCTGGATTGGTGAGAATGCTTGTGTGTGACAAGTATGAAGACTTGGGGCGCATGCATAGCTTGTTTCGTCGTGTTCCCATTGGGTTGCAAAC

Fln Alignment:

Contig15166___VEMDPKYAEKTRKIIEHAIHEIYNHNASGLSFEELYRNAYNMVLHKYGEKLYSGLVTTMTVHLKEIATVIEASQGGLFLEELNRQWMDHNKALQMIRDILMYMDRTFVTNSNKTPVNELGLNLWRDHIVKLPNIRDRLLNTLLDLVYTERTGEIIDRSLMRNVIKMLMDLGPSVYHDDFEKSFLDVTANFYRIESQQFIECCDCGDYMKKAERRLNEEMDRVSNYLDPKSEIKITSVVEREMIANHMTRLVHMENSGLVRMLVCDKYEDLGRMHSLFRRVPIGL
B8LNS8_______________VDMDPKYAEKTWKILEHAIHEIYNHNASGLSFEELYRNAYNMVLHKYGEKLYSGLVTTMTGHLREMAKTIEAAQGSLFLEGLNRKWVDHNKALQMIRDILMYMDRTYVTNSNKTPVHELGLNLWRDHIVRAAKIKDRLLNTLLELVRCERTGEVINRGLMRNIIKMLTELGPSVYHEDFEKPFVEEASTFYSIESQQFIECCDCGDYLQKAERRLNEEIERVSHYLDSKSEVKITAVVEREMIANHMQRLVHMENSGLVSMLVDDKYDDLGRMYNLFRRVPAGL

SNPs (tot: 15)

Position	A %	C %	G %	T %	Probability
330		66	33		0.997452
331	66		33		0.997452
422		33	66		0.997452
425		33	66		0.997452
426	66		33		0.997452
427		66		33	0.997452
432	66			33	0.997452
435	66		33		0.997452
437	66			33	0.997452
627		40		60	1.0
654	16	83			0.994512
655		83		16	0.994512
656	80		20		0.995493
657	80			20	0.995493
789	25		75		0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain