SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene15154

Length: 2374 nt

Origin of reads: Biogeco1 v2 Uagpf v2 Uma v2

UniGene Fasta

>sp_v2.0_unigene15154
TGATTCAGCTGGCTCATTCCTTCAATTTAGTGATGCAATGGATGAATTCGTTGATGTACCAGAAGACAGGCTTTTAATTTATTCTAGAACAGGAGGCTCTCTTCTAGGTCTAGTACCAGAAAGGAGTCTGCCCTCCTCAGAACAACTGCTGATTTGCGAGATTGGACTGGATGGCTGTCCATAATGCAGCCAACAGCTGATGTTGTTGCACCTTCACCATGGAGAATTTTTGGTTGTGAGAATGGATTGCGATTATTTGAAGAGGCAAGAGATGGGGAATTTAAAAGCAAGTGCTGGGGTGATCATCAAGCAGTGATGGCAGTTGGTGTTGTGGATGCAACCTGTGAATCAATATTTGAGACTGTTATGGCTCTTGGACCCTCGAGATTAGAGTGGGATTTTTGTTATCTCCGTGGTAAAGTTATTGAGCACTTGGATGGTCACACAGACATTGTTCACAAGCAGTTCCACAGTAACTGGCTTCCTTGGGGATTGACACCAAGAGATTTGCTACTACTTCGTTACTGGAGAAGAGAGGATGATGGCTCCTATGTGATACTTTATCATTCTGTGGAGCATAGGAAGTGTCCACCGTGTCGTGGCTTTGTGCGTGCCTGCATAAAAAGTGGAGGATATGTGATATCACCATTGACTCGTCCCCAACAACATGCATCTGCAGTTGTGAAACACATGCTTGCTGTAGATTGGAAATATTGGAAATCTTATTTACGATCTTCCTGTGAAAGGAATATAACTCTTAAAATGCTTGGGCGCATTGCAGCCATGAGGGAAATGTTCAAGGCCAAACTAGTACATTATCCATGTTCTGAGTTATCATCGTGTGAGCTAATTAATGAAAATGCGACAATGCAAATGGATGGAAGACATGTAAAAGAGGACACAAGCCCAATGACACAAGAAGAACAAAGCTCTGATGTTACATTGCAAGTACATGATACTTTCCTTCAAGCAAATGATTCAGCTGGCTCATTCCTTCAATTTAGTGATGCAATGGATGAATTCGTTGATGTACCAGAAGACTCAAACCATGGATCATCTGCACTAGATTCAGATCAGTGCTCAGAACAATTTATAGAGACTGAGGGGTATCCAGATTATGAACAAGTGTCTAAGTGCCAGTCTGGAGGGCCGAATAGGTTGTCAACAGCTGCAGTATTTGTCAAGCGCTTGCATGATCTTGCTTCTGCAGCTCAGAAAAGATCACAAGTGGACACAATTGGAGCGCAGGATGATGTAGATTTTCTTAGCTATGAAGCAACACTTCCCAAAAACTCTGCTTGTACATTGCCAAGCAGCTGGGCCATTGCTGATCCAGCTACATTTTTAATCCGTGGCAAGAGTTATTTGCAAGATCGTCAAAAAATTAAGGCAAAGGATACCTTGATGCGTATGGTTGCAGCAGACTGGCTCAGGTCTGATAAGCGAGAGGACAATCTTGCTGGTCGCCCAGAATCCTTTATTCAGAGGAGAGCATCTCAGGGTGGAAAAGAATTCTTTTTTGTTGTAAATATCCAGGTGCCCAGGTGCAACAACATACTCTCTTGCCTTATACTACATGATGGATTGTCCTATTGAAAATGTTCCTTTGCTAGAAAATTTTGTCAAAGGAGATGATGCTTATCGCAATTCACGATTCAAAATCATTCCCCATAGTGCCAAGGCTTCCTCAATTGTGAAGCAGAGTGTAGGCAAAACTGCATGTTTGGTGGGTCAAGCACTAGAAATCAATTATCCTAGGGGAAGCAACTATCTTGAGCTGGATGTTAATATCGGTTCATCTTCAGTAGCCAAAGGTGTCGTTAACCTAGTGCTTGGCTACCTGAGTAAGTTGGTTATCGAAATGGCCTTTGTGATACAGGCAAACACGGAAGATGAGCTCCCTGAGTTTCTTCTAGGGACATGCAGGCTCAATCATCTGGATGCGTCTAAATCGATTCCAGCTGACCAGAAATATTCTCAAGCTGCTTAATGTTAAGTTCCACTAAAAAAACCTCACCGATAACCATCAGAAAATTTATTATTGGAAAATCAGCCACATTCTAAATTTTAATCTGGGGAAACAAGGCAGGAATTGTTGTTAGTGTCAATTAAAAAATACGGAGGTTCAAGAATAATGAAAAATGAATTTTACATCATCTTCCAGGGTGGTTTCCAAATAATGGCCTTCATTTGGTGTTGTATATGTTATCACCAGAGCTCTTCACTAAGTTTGCAAAGATCGACGTCAAGTATACGTCATATCCATCAAACATTTTCTTTGAAAATTCTTGTAATCACATTGCATTTTTAACTACATTGCAATTGATGACAGCTGTCAATTAATTGCCTTAAAAAATCTACGGACTATATAGAA

Ace file of the UniGene sp_v2.0_unigene15154

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	pleckstrin homology (PH) and lipid-binding START domain-containing protein [Arabidopsis thaliana] gb\|AEC08107.1\| pleckstrin homology (PH) and lipid-binding START domain-containing protein [Arabidopsis thaliana]	-	-	51%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	32%
Blast2go	pleckstrin homology and lipid-binding start domain-containing protein	-	-	67%

Source	GOs	Term	Type	e value	Identity
Blast2go	plasma membrane	GO:0005886	Cellular Component	0.0	67%

Source	InterPros	Term	Type	Identity
Sma3	ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain	IPR000194	-	-
Sma3	GPCR, rhodopsin-like, 7TM	IPR000276	-	-
Sma3	Pleckstrin homology domain	IPR001849	-	-
Sma3	Lipid-binding START	IPR002913	-	-
Sma3	2-oxo acid dehydrogenase, lipoyl-binding site	IPR003016	-	-
Sma3	Domain of unknown function DUF1336	IPR009769	-	-
Sma3	Pleckstrin homology-like domain	IPR011993	-	-
Sma3	Reverse transcriptase, RNA-dependent DNA polymerase	IPR013103	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: A9NVR7

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 183 aas, putative chimeric sequence, your sequence is longer than subject: 471 - 320

Fln protein: MQPTADVVAPSPWRIFGCENGLRLFEEARDGEFKSKCWGDHQAVMAVGVVDATCESIFETVMALGPSRLEWDFCYLRGKVIEHLDGHTDIVHKQFHSNWLPWGLTPRDLLLLRYWRREDDGSYVILYHSVEHRKCPPCRGFVRACIKSGGYVISPLTRPQQHASAVVKHMLAVDWKYWKSYLRSSCERNITLKMLGRIAAMREMFKAKLVHYPCSELSSCELINENATMQMDGRHVKEDTSPMTQEEQSSDVTLQVHDTFLQANDSAGSFLQFSDAMDEFVDVPEDSNHGSSALDSDQCSEQFIETEGYPDYEQVSKCQSGGPNRLSTAAVFVKRLHDLASAAQKRSQVDTIGAQDDVDFLSYEATLPKNSACTLPSSWAIADPATFLIRGKSYLQDRQKIKAKDTLMRMVAADWLRSDKREDNLAGRPESFIQRRASQGGKEFFFVVNIQVPRCNNILSCLILHDGLSY*

Protein Length: 472

Fln nts: TGATTCAGCTGGCTCATTCCTTCAATTTAGTGATGCAATGGATGAATTCGTTGATGTACCAGAAGACAGGCTTTTAATTTATTCTAGAACAGGAGGCTCTCTTCTAGGTCTAGTACCAGAAAGGAGTCTGCCCTCCTCAGAACAACTGCTGATTTGCGAGATTGGACTGGATGGCTGTCCATA_-_ATGCAGCCAACAGCTGATGTTGTTGCACCTTCACCATGGAGAATTTTTGGTTGTGAGAATGGATTGCGATTATTTGAAGAGGCAAGAGATGGGGAATTTAAAAGCAAGTGCTGGGGTGATCATCAAGCAGTGATGGCAGTTGGTGTTGTGGATGCAACCTGTGAATCAATATTTGAGACTGTTATGGCTCTTGGACCCTCGAGATTAGAGTGGGATTTTTGTTATCTCCGTGGTAAAGTTATTGAGCACTTGGATGGTCACACAGACATTGTTCACAAGCAGTTCCACAGTAACTGGCTTCCTTGGGGATTGACACCAAGAGATTTGCTACTACTTCGTTACTGGAGAAGAGAGGATGATGGCTCCTATGTGATACTTTATCATTCTGTGGAGCATAGGAAGTGTCCACCGTGTCGTGGCTTTGTGCGTGCCTGCATAAAAAGTGGAGGATATGTGATATCACCATTGACTCGTCCCCAACAACATGCATCTGCAGTTGTGAAACACATGCTTGCTGTAGATTGGAAATATTGGAAATCTTATTTACGATCTTCCTGTGAAAGGAATATAACTCTTAAAATGCTTGGGCGCATTGCAGCCATGAGGGAAATGTTCAAGGCCAAACTAGTACATTATCCATGTTCTGAGTTATCATCGTGTGAGCTAATTAATGAAAATGCGACAATGCAAATGGATGGAAGACATGTAAAAGAGGACACAAGCCCAATGACACAAGAAGAACAAAGCTCTGATGTTACATTGCAAGTACATGATACTTTCCTTCAAGCAAATGATTCAGCTGGCTCATTCCTTCAATTTAGTGATGCAATGGATGAATTCGTTGATGTACCAGAAGACTCAAACCATGGATCATCTGCACTAGATTCAGATCAGTGCTCAGAACAATTTATAGAGACTGAGGGGTATCCAGATTATGAACAAGTGTCTAAGTGCCAGTCTGGAGGGCCGAATAGGTTGTCAACAGCTGCAGTATTTGTCAAGCGCTTGCATGATCTTGCTTCTGCAGCTCAGAAAAGATCACAAGTGGACACAATTGGAGCGCAGGATGATGTAGATTTTCTTAGCTATGAAGCAACACTTCCCAAAAACTCTGCTTGTACATTGCCAAGCAGCTGGGCCATTGCTGATCCAGCTACATTTTTAATCCGTGGCAAGAGTTATTTGCAAGATCGTCAAAAAATTAAGGCAAAGGATACCTTGATGCGTATGGTTGCAGCAGACTGGCTCAGGTCTGATAAGCGAGAGGACAATCTTGCTGGTCGCCCAGAATCCTTTATTCAGAGGAGAGCATCTCAGGGTGGAAAAGAATTCTTTTTTGTTGTAAATATCCAGGTGCCCAGGTGCAACAACATACTCTCTTGCCTTATACTACATGATGGATTGTCCTATTGA___AAATGTTCCTTTGCTAGAAAATTTTGTCAAAGGAGATGATGCTTATCGCAATTCACGATTCAAAATCATTCCCCATAGTGCCAAGGCTTCCTCAATTGTGAAGCAGAGTGTAGGCAAAACTGCATGTTTGGTGGGTCAAGCACTAGAAATCAATTATCCTAGGGGAAGCAACTATCTTGAGCTGGATGTTAATATCGGTTCATCTTCAGTAGCCAAAGGTGTCGTTAACCTAGTGCTTGGCTACCTGAGTAAGTTGGTTATCGAAATGGCCTTTGTGATACAGGCAAACACGGAAGATGAGCTCCCTGAGTTTCTTCTAGGGACATGCAGGCTCAATCATCTGGATGCGTCTAAATCGATTCCAGCTGACCAGAAATATTCTCAAGCTGCTTAATGTTAAGTTCCACTAAAAAAACCTCACCGATAACCATCAGAAAATTTATTATTGGAAAATCAGCCACATTCTAAATTTTAATCTGGGGAAACAAGGCAGGAATTGTTGTTAGTGTCAATTAAAAAATACGGAGGTTCAAGAATAATGAAAAATGAATTTTACATCATCTTCCAGGGTGGTTTCCAAATAATGGCCTTCATTTGGTGTTGTATATGTTATCACCAGAGCTCTTCACTAAGTTTGCAAAGATCGACGTCAAGTATACGTCATATCCATCAAACATTTTCTTTGAAAATTCTTGTAATCACATTGCATTTTTAACTACATTGCAATTGATGACAGCTGTCAATTAATTGCCTTAAAAAATCTACGGACTATATAGAA

Fln Alignment:

Contig15154___SSWAIADPATFLIRGKSYLQDRQKIKAKDTLMRMVAADWLRSDKREDNLAGRPESFIQRRAS------QGGKEFFFVVNIQVP
A9NVR7_______________NGWSSPPGNLFHVRGSNYFTKKQKVPAGDWLLKPLGMDWLKAGSKLDHVLGRPDNRVMAALSKANSDGKGLKTFVFAVNLQVP

SNPs (tot: 44)

Position	A %	C %	G %	T %	Probability
230	25			75	0.996473
308	66		33		0.997452
629	66		33		0.997452
1565	42	57			1.0
1588		11		88	0.923564
1590			88	11	0.923564
1591	88	11			0.923564
1592			11	88	0.923564
1595	11			88	0.923564
1601			11	88	0.923564
1603	88	11			0.923564
1604	88			11	0.923564
1605	88		11		0.923564
1606	88			11	0.923564
1607		11		88	0.923564
1608	11		88		0.923564
1609		11		88	0.923564
1612	11	88			0.923564
1801		10	90		0.90298
1802			10	90	0.90298
1804		90	10		0.90298
1806		10		90	0.90298
1810		88	11		0.923564
1811	88		11		0.923564
1812		11	88		0.923564
1816		88		11	0.923564
1819	87			12	0.992554
1820	87	12			0.992554
1821	12		87		0.992554
1824			87	12	0.992554
2065		85		14	0.993533
2111	16		83		0.994512
2119	16			83	0.994512
2121	83			16	0.994512
2122	83		16		0.994512
2185	83			16	0.994512
2193	16		83		0.994512
2224	50	50			1.0
2230	16	83			0.994512
2245	50		50		1.0
2254		40		60	1.0
2291	33		66		0.997452
2295	66			33	0.997452
2320	66			33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain