SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene15064

Length: 1451 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene15064

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Glycerol-3-phosphate acyltransferase n=2 Tax=Citrus RepID=Q8W3L0_CITUN	-	-	71%
FL-Next	sp=Glycerol-3-phosphate acyltransferase, chloroplastic; Arabidopsis thaliana (Mouse-ear cress).	-	-	40%
Blast2go	glycerol-3-phosphate acyltransferase	-	-	85%

Source	ECs	Term	Type	e value	Identity
Blast2go	Heme oxygenase.	EC:1.14.99.3	-	0.0	85%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Porphyrin and chlorophyll metabolism	00860		0.0	85%
Blast2go	Glycerol-3-phosphate O-acyltransferase.	EC:2.3.1.15	-	0.0	85%

Source	KEGGs	Term	Identity
Blast2go	Glycerolipid metabolism	00561	85%
Blast2go	Glycerophospholipid metabolism	00564	85%
Blast2go	Metabolic pathways	01100	85%

Source	GOs	Term	Type	Identity
Blast2go	phosphatidylglycerol biosynthetic process	GO:0006655	Biological Process	85%
Blast2go	lipid particle organization	GO:0034389	Biological Process	85%
Blast2go	oxylipin biosynthetic process	GO:0031408	Biological Process	85%
Blast2go	calcium ion binding	GO:0005509	Molecular Function	85%
Blast2go	heme oxygenase (decyclizing) activity	GO:0004392	Molecular Function	85%
Blast2go	linoleic acid epoxygenase activity	GO:0071614	Molecular Function	85%
Blast2go	glycerol-3-phosphate O-acyltransferase activity	GO:0004366	Molecular Function	85%
Blast2go	integral to membrane	GO:0016021	Cellular Component	85%
Blast2go	chloroplast stroma	GO:0009570	Cellular Component	85%

Source	InterPros	Term	Type	e value	Identity
Sma3	Phospholipid/glycerol acyltransferase	IPR002123	-	0.0	-
Sma3	Glycerol-3-phosphate O-acyltransferase, chloroplast	IPR016222	-	0.0	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: sp_plants

Fln subject: Q43307

Fln msg: Overlapping hits, possible frame ERROR between 419 and 404, query STOP codon too far from subject stop. Distance to subject end: 36 aas, putative chimeric sequence, atg_distance in limit (1-15): atg_distance = 16, No stop codon before M and low homology subject, your sequence is shorter than subject: 406 - 459

Fln protein: MPLHGRATLCFLAAHRQSRGLGFPRLKLNSSSRNKMRIKATTELVEHIGNIEQVVGPPKNLAVAEQPLKTDNSAGHSQRFLNVKTEEDLFRGIREEIEAKRLPASAAASMEEVYRNYHDAVLCSGxxxxxxxVVSNMAVTFDRILLQFEDPFTFSSYHKAIRDPFDYYMFGQNYIRPLIDFRNSYVGNLALFDEMDKQLKQGHNIVLFSNHQSEADPAVIALMLETSNPNIAEKITYVAGDRVVLDPICKPFSMGRNLLCVYSKKHIEDVPELAEMKKKANARTLKEMALLLRKGGQVIWIAPSGGRDRPDPDTKEWFPASFDVAAVDNMRRLVEHSGVPGHLYPLALLCYDIMPPPPQVEREIGEQRKIGFHGVGISVASEISFSEVVAGIENQEEVHAVEYED*

Protein Length: 407

Fln nts: AATGTGGGTTTAATTTATCTTCCGAG_-_ATGCCATTACACGGCAGAGCTACTTTGTGTTTCTTGGCGGCTCATCGCCAGTCCAGGGGCTTGGGCTTCCCCCGTTTAAAGTTAAATTCTAGCTCAAGGAACAAGATGAGAATAAAGGCAACGACGGAGCTGGTGGAACACATAGGCAATATTGAGCAAGTTGTGGGTCCTCCTAAAAATTTGGCCGTTGCAGAGCAGCCACTGAAGACAGACAATTCTGCCGGACACTCCCAGAGGTTTCTGAACGTCAAAACGGAGGAAGATCTTTTCAGGGGAATTAGAGAGGAAATTGAAGCCAAACGATTACCTGCTAGTGCTGCAGCCTCGATGGAGGAGGTATATCGAAATTATCATGATGCCGTTCTTTGCAGCGGTxxxxxxxxxxxxxxxxxxxxxGTTGTTTCAAACATGGCAGTAACTTTTGATCGTATTCTCTTGCAATTTGAGGATCCTTTTACATTTTCTTCCTATCACAAAGCAATACGGGATCCTTTTGATTACTACATGTTTGGACAAAATTACATCCGCCCATTGATTGATTTCAGGAACTCATATGTTGGGAATTTAGCGTTATTTGATGAGATGGACAAACAGTTAAAGCAGGGTCATAACATTGTACTGTTTTCAAATCATCAATCAGAAGCTGATCCTGCAGTTATCGCATTAATGCTGGAAACTAGTAATCCAAATATAGCAGAGAAGATAACATATGTAGCGGGAGATAGGGTGGTGTTGGATCCAATTTGTAAGCCTTTCAGTATGGGCAGGAATCTACTATGCGTGTACTCAAAGAAGCACATAGAAGATGTACCAGAACTTGCTGAAATGAAAAAGAAAGCAAATGCACGGACATTAAAGGAGATGGCTTTGCTTCTGAGGAAGGGTGGCCAAGTCATCTGGATTGCTCCAAGTGGTGGTAGAGACCGTCCTGATCCAGATACAAAGGAATGGTTTCCAGCTTCTTTTGATGTTGCAGCAGTGGACAATATGAGGAGACTTGTGGAGCATTCTGGTGTTCCTGGGCACTTGTATCCTTTGGCATTGCTTTGCTATGACATCATGCCACCTCCACCTCAGGTTGAAAGGGAAATAGGTGAGCAGAGAAAAATTGGATTTCATGGAGTTGGGATCTCTGTGGCTTCTGAAATAAGTTTCAGTGAAGTTGTTGCTGGTATTGAAAATCAAGAGGAGGTACATGCAGTTGAATACGAAGATTAACCATGCCGACAGCTTGAAGCTGAAAAATGTCCCAACGCCAACTTCTTATTTTATTATAGGGATCTCCTCCAATTGAACATGAAGATCGACATTTAATATAAGATATAATCTACGCATTTTGGTATTTCCTGGCCGCTAGTAACTACGCTAGTGCGGAAATACAATTCTTATGGTAATGTGTGACATTACAATGTTACTGCTACTTGAT

Fln Alignment:

Contig15064___SRNKMRIKATTELVEHIGNIEQVVGPPKNLAVAEQPLKTDNSAGHSQRFLNVKTEEDLFRGIREEIEAKRLPASAAASMEEVYRNYHDAVLCSGDxxxxxIVVSNMAVTFDRILLQFEDPFTFSSYHKAIRDPFDYYMFGQNYIRPLIDFRNSYVGNLALFDEMDKQLKQGHNIVLFSNHQSEADPAVIALMLETSNPNIAEKITYVAGDRVVLDPICKPFSMGRNLLCVYSKKHIEDVPELAEMKKKANARTLKEMALLLRKGGQVIWIAPSGGRDRPDPDTKEWFPASFDVAAVDNMRRLVEHSGVPGHLYPLALLCYDIMPPPPQVEREIGEQRKIGFHGVGISVASEISFSEVVAGIENQEE
Q43307_______________SRGGVSVRAMSELVQ-----DKESSVAASIAFNEAAGETPSELSHSRTFLDARSEQDLLSGIKKEAEAGRLPANVAAGMEELYWNYKNAVLSSGAxxxxxTVVSNMSVAFDRMLLGVEDPYTFNPYHKAVREPFDYYMFVHTYIRPLIDFKNSYVGNASIFSELEDKIRQGHNIVLISNHQSEADPAVISLLLEAQSPFIGENIKCVAGDRVITDPLCKPFSMGRNLICVYSKKHMNDDPELVDMKRKANTRSLKEMATMLRSGGQLIWIAPSGGRDRPNPSTGEWFPAPFDASSVDNMRRLVEHSGAPGHIYPMSLLCYDIMPPPPQVEKEIGEKRLVGFHGTGLSIAPEINFSDVTADCESPNE

SNPs (tot: 37)

Position	A %	C %	G %	T %	Probability
243			25	75	0.996473
276	80		20		0.995493
277	80			20	0.995493
278	80		20		0.995493
281		20	80		0.995493
282			80	20	0.995493
283	80			20	0.995493
286	80	20			0.995493
287	80			20	0.995493
292	20			80	0.995493
293		20		80	0.995493
295		20		80	0.995493
296	20	80			0.995493
348	20		80		0.995493
1049	25			75	0.996473
1050	25		75		0.996473
1051			75	25	0.996473
1053		75		25	0.996473
1224	50	50			1.0
1225	80			20	0.995493
1226			40	60	1.0
1228	40	60			1.0
1231		40		60	1.0
1233			75	25	0.996473
1234	75			25	0.996473
1235	75	25			0.996473
1238		75		25	0.996473
1239	25		75		0.996473
1242	25		75		0.996473
1246	75			25	0.996473
1247	75			25	0.996473
1248	25	75			0.996473
1249		75		25	0.996473
1254	25	75			0.996473
1255			75	25	0.996473
1264	75			25	0.996473
1265	75			25	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain