SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene14557

Length: 1437 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene14557

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	asparaginyl-tRNA synthetase [Arabidopsis thaliana] sp\|O48593.3\|SYNO_ARATH RecName: Full=Asparaginyl-tRNA synthetase, chloroplastic/mitochondrial; AltName: Full=Asparagine--tRNA ligase; Short=AsnRS; Flags: Precursor gb\|AAL84964.1\| AT4g17300/dl4685w [Arabid	-	-	81%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	28%
Blast2go	protein	-	-	88%

Source	GOs	Term	Type	Identity
Blast2go	ovule development	GO:0048481	Biological Process	88%
Blast2go	tRNA aminoacylation for protein translation	GO:0006418	Biological Process	88%
Blast2go	aminoacyl-tRNA ligase activity	GO:0004812	Molecular Function	88%
Blast2go	mitochondrion	GO:0005739	Cellular Component	88%
Blast2go	chloroplast	GO:0009507	Cellular Component	88%

Source	InterPros	Term	Type	Identity
Sma3		IPR000173	-	-
Sma3	WHEP-TRS	IPR000738	-	-
Sma3	Helicase, C-terminal	IPR001650	-	-
Sma3	Aspartyl/Asparaginyl-tRNA synthetase, class IIb	IPR002312	-	-
Sma3	2-oxo acid dehydrogenase, lipoyl-binding site	IPR003016	-	-
Sma3	Aminoacyl-tRNA synthetase, class II (D/K/N)	IPR004364	-	-
Sma3	Nucleic acid binding, OB-fold, tRNA/helicase-type	IPR004365	-	-
Sma3	Asparaginyl-tRNA synthetase, class IIb	IPR004522	-	-
Sma3	Aminoacyl-tRNA synthetase, class II	IPR006195	-	-
Sma3	Protein of unknown function DUF821, CAP10-like	IPR008539	-	-
Sma3	Nucleic acid-binding, OB-fold	IPR012340	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-
Sma3		IPR017442	-	-
Sma3	ABC transporter, conserved site	IPR017871	-	-
Sma3	Aminoacyl-tRNA synthetase, class II (D/K/N)-like	IPR018150	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NX07

Fln msg: your sequence is shorter than subject: 322 - 566

Fln protein: CVWVASPIITASDCEGAGEQFCVTSLLPSSKEDVGSPISMIPSTKDGYVDWAQDFFGKPAFLTVSGQLNGETYACALSDVYTFGPTFRAEQSNTSRHLAEFWMIEPELAFANLNDDMACATAYLQYVVRFILDNCKEDMEFFNTWIEKGIITRLSNVAEKEFVQLTYTDAVELLLKAKKNFEFPVTWGCDLQSEHERYITEEAFSGSPVIIRDYPKDIKAFYMRENADGKTVAAMDVLVPRVGELIGGSQREERLELLENRMEQLGLRKESYWWYLDLRRYGSVPHAGFGLGFERLVQFATGMENIRDVIPFPRTPGSAEF*

Protein Length: 323

Fln nts: AATGTGTCTGGGTTGCCAGTCCAATAATCACCGCTTCAGATTGTGAAGGTGCCGGAGAGCAATTTTGTGTCACATCTTTGCTTCCTAGCTCTAAGGAGGATGTGGGCTCTCCAATTAGCATGATCCCTAGCACAAAGGATGGTTATGTTGACTGGGCACAGGATTTTTTTGGAAAACCAGCATTCTTGACAGTTTCTGGACAGCTAAATGGAGAGACCTATGCATGTGCTCTTTCAGATGTATACACGTTTGGTCCCACATTTCGAGCAGAGCAGTCGAACACTTCACGTCATTTAGCTGAGTTCTGGATGATTGAACCAGAACTTGCTTTTGCCAATCTGAATGATGACATGGCTTGTGCAACTGCCTATCTCCAGTATGTCGTGAGGTTTATTCTGGATAATTGCAAAGAAGATATGGAATTCTTTAATACCTGGATTGAGAAGGGGATAATTACCCGTTTAAGTAATGTTGCAGAAAAGGAATTCGTGCAATTGACATATACAGATGCAGTTGAACTGCTTTTGAAAGCAAAGAAAAATTTTGAATTCCCGGTGACATGGGGATGTGACTTGCAGAGTGAGCATGAGCGTTATATAACAGAGGAAGCCTTTTCTGGTTCACCTGTTATTATAAGAGATTATCCCAAAGATATAAAAGCTTTCTATATGCGAGAAAATGCAGATGGGAAGACTGTAGCAGCTATGGATGTTTTAGTTCCAAGGGTGGGTGAGCTCATTGGTGGAAGCCAACGTGAGGAACGCCTTGAGTTACTTGAGAATCGAATGGAACAGTTGGGGCTCAGAAAGGAAAGTTACTGGTGGTATCTAGATTTGCGACGTTACGGTTCAGTTCCTCATGCTGGGTTTGGATTGGGTTTCGAGAGGCTTGTCCAATTTGCAACTGGAATGGAGAACATCCGAGATGTTATACCATTTCCAAGAACTCCTGGCTCTGCAGAATTTTAG___ATGACCTCCCACGGTGGTGTAGAGTGAGAACAAATATTGATATGCTTTCAAAGAGTTCTTGAAAAGGAGAAAGAGAAATTAGAAATCAAATGTTGATCAGCGTCTCTAGTCATTGTCCTTAATGAATATGATAAGCAATTTGAGATCAAATTAAACGTAGTTGTTTTAGGTAAAATCAGGTGCTTTTTCCATACTTTCAAAACATATTATAACGCTCCTGTAATTGTTTGAGTGGATAACTATTGCCGTGCTAACCAACTATACGAACTAATTACTAATCTGTTAATGTATATATATTTAATGGTGATTGTGCATCGTAGATGCTTTTAGATTTAGTAATAAATAAGTCAAGATGACTTGAGGTGTTTCATCTCCCATGAGACTCTTGATCTTTCAGCCATGGGACTTGAGATGCAGTAGAATCATATTGATCATTTTTTAATAATGAATTGAAGTGATTATTCTCACT

Fln Alignment:

Contig14557___DVGSPISMIPSTKDGYVDWAQDFFGKPAFLTVSGQLNGETYACA-LSDVYTFGPTFRAEQSNTSRHLAEFWMIEPELAFANLNDDMACATAYLQYVVRF--ILDNCKEDMEFFNTWIEKGIITRLSNVAEKEFVQLTYTDAVELLLKAKKNFEFPVTWGCDLQSEHERYI---TEEAFSGSPVIIRDYPKDIKAFYMRENADGKTVA-AMDVLVPRVGELIGGSQXXXXXXXXXXXXXQLGLRKESYWWYLDLRRYGSVPHAGFGLGFERLVQFATGMENIRDVIPFPRTP
A9NX07_______________ELHTPKLIAGASEGGSSVFKLDYKGQPACLAQSPQLHKQMAICADFGRVFEIGPVFRAEDSFTHRHLCEFTGLDLEMEIKEHYFEVLDILDKL-FITIFDGLNEHCKKELHAINQQYPFEPLKYL-----RKTLRLTFQEGVQMLKEAGIEVD-PLG---DFNTETERKLGGLVREKYDTDFYILHRYPLAVRPFYTMPCIDDPLYSNSFDVFI-RGEEIISGAQRVHEPEFLTKRAKELGIDVNTISTYIDSFRWGAVPHGGLGVGLERVVMLFCALNNIRKTSLFPRDP

SNPs (tot: 23)

Position	A %	C %	G %	T %	Probability
146			20	80	0.995493
341		80	20		0.995493
394			33	66	0.997452
1049	83	16			0.994512
1050	83	16			0.994512
1053	83		16		0.994512
1055	83	16			0.994512
1056	83			16	0.994512
1057	83		16		0.994512
1061	85	14			0.993533
1062	71	14	14		0.984337
1064		14	71		0.984337
1065		14	14	71	0.984337
1066	14			85	0.993533
1069		14		85	0.993533
1071	85			14	0.993533
1073		85	14		0.993533
1074		14	85		0.993533
1076		85	14		0.993533
1081		14	85		0.993533
1173	85		14		0.993533
1248			50	50	1.0
1283		14		85	0.993533

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain