SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene14504

Length: 2730 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene14504
ATGGGGGTGGGATCAACACATAAATATGCGTCGTGACCTTGGCTGACATGGGGGAGTGCAGATATAATTTGATTCCGAGCATCGCAGGAGACATGTGGTCAAACTTCACCTGTTATTTCTCCATATTCCTGGGTGCTCCTCGGCTCCGCAGCAATTCAAGCTAAAATTACCTTTAAGTCCGACAGAATTCACTGTGCTCTAACAGGAATTTAACTGGGAATGGATTGTGTAGATGAATGGGGAGCCTCAAAATTGGGACCATCTCTGATTCTCAGAGCTCTAAATTTATCAACATGGCAGTATTTTGTTGGGCTGACGTCAATTTTAATTTATTTCCTATATAATTTTCTGGAAATGCATTTGATTGGGGATCTGTTAGTGGGGATGAGAAGGAACTCTGTAAAGCTATTGTACGACCCGTCATCCAACATCGCTACTCACTTGTTACCGAAGTGCGAGATCCTCAAGGGAAGGTATTGGTCAACACCTTGGCTTTGCAGCCCTCATTTGCAAACAGCTTTTCTCCACTTTTTTGGCAGACCGCCCATGTTTCAGTACCGTAGGCAGATTTTCCTTCCGCCAGATGGGGGCACTGTTGCTCTAGATTGGCTCACAACTGAAGATATTGTTGGAGGAGCTGCCAACCTGAACACACTTGCCTCAGTGCATGATGAAACACCAATAGTGATTGTGATTCCTGGCCTGACCAGTGATTCCAAAGATCCTTATATAAAGCACATCGTTTATGCTCTTGCCAAGCAAGGATGGAAAACTGTTGTTGCTAATCATCGAGGGCTGGGAGGAACTTCCATTACTTCAGATCGCTTTTACAATGCTGGGTGGACAGAAGATCTGCGGAACATTATTGCTTATGTGCATCATGAGCATCCTAAAGCGCCTTTGCTAGCAGTTGGGACAAGTATCGGTGCGAATATACTGGTGAAATATCTTGGCGAGGAGGGAGAGAATACTCCACTAGCTGGAGGTGCAGCCATATGCTGCCCTTGGGACCTTGTGATATGTGGTCGCTTTATAAAGAGGAGCTTTGTCCAAAGACTATATGACAAAATTCTGACTATTGGTCTTCGAGGTTATGCTCAGCTACATCAGAGTACTGTTAGCCGGCTTGCAGATTGGGAATGTATAAAAAAGTCACACTCTATAAGAGATTTTGACAATTATTTTACCTGCCGTATTGGTAAATTTGAGACAGTGGATACATATTATCGCAAATGCAGCAGTGCCCAATTTCTCAGTAATGTGTCAGTGCCGTTACTTTGTGTGAATGCCTTGGATGATCCTGTTTGCACAAAAGAGGCCATTCCATGGGATGAATGCAGGGCAAATAAAAATATTGTTCTTGCCACTACACTACATGGAGGACATCTTGCATTCTTTGAGGGATTAATGGCGGGTAGTATCTGGTGGGTACGGGCTGTAAAGGAATTTCTCTCTGTACTTCATTCAAGTTCTCTTATGCATACCCAGAAAAAGGTACAAACTTCTGGTCTGAGCTCAACGCTTACATCTTCTATTGACAAAGGGCCCTATTTGAATGTCTCTGAAGATGGAATGGTTAGCACCGACAATAATATTTGTATGGATTCTGAAGGGGATGACAGAGTTATGGAAGAATTACTTTTACCTTCAAAATCATCTATGAAAATATCGGGTGAAAATGAAGGTGTAGATGATGAACAACCACATGCTGCAACGGACACTCCCTTTATGCAAGAACCAAAGCTTGAAGAAGGCAGTGTAGAATATTCTCAGCTTAAGCTCTCAAATGGGGATAAAGGGTCTATATTTGAAATACGCATTAAAAGTTGTGCAGAGTTCATTGTTCTTCAATCTGTTTTACGACAACTTTTGAACCAGATGCAGGCTACTACACAAGGTAAAACTGTATTGAATGTGGGGCCAAAAAATTCAGAAACTGATGTTTTTTCTCAATTTGGAGGAAACAAGGTCATAGATGTACACAAGACTTCTGAAGATGATTTAACTTTCTGCAACTCTGATGGTTGTGCACATGTAAATGCGAAAACTGGTCCATCAAACGAACATGTAGTGCAGAAGCAAAGAACCAGCGATGACATTTCAAATAAATTAGTACCTATAAAGGCCATAATGCAACTTTCTCGGCAACAAAAGCGTTCGATGTGGATTCTGGCCTATATAGCCGTTGTAACTACATGGCCTTTAGTCGGTTCAGTTCTCGTGTTTAATTTTAAAAGAAAAGTTAAAAACATGTGGAGTGCAACATGGCATGGAAAATAGTTGTCCCTTTAGGATTGCTCAAGTATGGTACATTGACAGATGTAACCGTCTGCTACGAGTACAGTTTCTCAATTTATGCCCATATGAATCCCTTTTCCGTTATCGTATGTTGTCATATTGTTTGGAGAACATGCCCCCATCCCTGAAATAATGAACTTCGTATATAATGATTAAATTCACTTTAATATCCTGTCATTATTTTACGTAGAATGAACACAATCTTTTTACATTAGTTCACCTTTTACTCAAGGAAGATTAAATGTCGTGTATATAAGTATATGGTTGAGCCGTTGCACGTGAGTGCATGGTTTAGCGGTCGGGGCTGATGGCTTATTCGCTCCCCACCCGCGTTCGATACCTATCGTGATGCTGGCACAACAGCCATTTGTATCATCGTGAATGTATATTGATATATGGTGTTTGATGTTGATTGATATGACAGATATCTAG

Ace file of the UniGene sp_v2.0_unigene14504 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	alpha/beta-hydrolase domain-containing protein [Arabidopsis thaliana] gb\|AAD39602.1\|AC007454_1 Contains a PF\|00561 alpha/beta hydrolase fold domain [Arabidopsis thaliana] gb\|AAG51902.1\|AC023913_10 late embryogenesis abundant protein, putative; 78257-75162	-	-	59%
FL-Next	sp=Embryogenesis-associated protein EMB8; Picea glauca (White spruce) (Pinus glauca).	-	-	35%
Blast2go	embryogenesis-associated protein emb8	-	-	72%

Source	InterPros	Term	Type	Identity
Sma3	Alpha/beta hydrolase fold-1	IPR000073	-	-
Sma3	Aminoacyl-tRNA synthetase, class I, conserved site	IPR001412	-	-
Sma3	AB-hydrolase YheT, putative	IPR012020	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: Q40863

Fln msg: your sequence is longer than subject: 687 - 457

Fln protein: MDCVDEWGASKLGPSLILRALNLSTWQYFVGLTSILIYFLYNFLEMHLIGDLLVGMRRNSVKLLYDPSSNIATHLLPKCEILKGRYWSTPWLCSPHLQTAFLHFFGRPPMFQYRRQIFLPPDGGTVALDWLTTEDIVGGAANLNTLASVHDETPIVIVIPGLTSDSKDPYIKHIVYALAKQGWKTVVANHRGLGGTSITSDRFYNAGWTEDLRNIIAYVHHEHPKAPLLAVGTSIGANILVKYLGEEGENTPLAGGAAICCPWDLVICGRFIKRSFVQRLYDKILTIGLRGYAQLHQSTVSRLADWECIKKSHSIRDFDNYFTCRIGKFETVDTYYRKCSSAQFLSNVSVPLLCVNALDDPVCTKEAIPWDECRANKNIVLATTLHGGHLAFFEGLMAGSIWWVRAVKEFLSVLHSSSLMHTQKKVQTSGLSSTLTSSIDKGPYLNVSEDGMVSTDNNICMDSEGDDRVMEELLLPSKSSMKISGENEGVDDEQPHAATDTPFMQEPKLEEGSVEYSQLKLSNGDKGSIFEIRIKSCAEFIVLQSVLRQLLNQMQATTQGKTVLNVGPKNSETDVFSQFGGNKVIDVHKTSEDDLTFCNSDGCAHVNAKTGPSNEHVVQKQRTSDDISNKLVPIKAIMQLSRQQKRSMWILAYIAVVTTWPLVGSVLVFNFKRKVKNMWSATWHGK*

Protein Length: 688

Fln nts: ATGGGGGTGGGATCAACACATAAATATGCGTCGTGACCTTGGCTGACATGGGGGAGTGCAGATATAATTTGATTCCGAGCATCGCAGGAGACATGTGGTCAAACTTCACCTGTTATTTCTCCATATTCCTGGGTGCTCCTCGGCTCCGCAGCAATTCAAGCTAAAATTACCTTTAAGTCCGACAGAATTCACTGTGCTCTAACAGGAATTTAACTGGGA_-_ATGGATTGTGTAGATGAATGGGGAGCCTCAAAATTGGGACCATCTCTGATTCTCAGAGCTCTAAATTTATCAACATGGCAGTATTTTGTTGGGCTGACGTCAATTTTAATTTATTTCCTATATAATTTTCTGGAAATGCATTTGATTGGGGATCTGTTAGTGGGGATGAGAAGGAACTCTGTAAAGCTATTGTACGACCCGTCATCCAACATCGCTACTCACTTGTTACCGAAGTGCGAGATCCTCAAGGGAAGGTATTGGTCAACACCTTGGCTTTGCAGCCCTCATTTGCAAACAGCTTTTCTCCACTTTTTTGGCAGACCGCCCATGTTTCAGTACCGTAGGCAGATTTTCCTTCCGCCAGATGGGGGCACTGTTGCTCTAGATTGGCTCACAACTGAAGATATTGTTGGAGGAGCTGCCAACCTGAACACACTTGCCTCAGTGCATGATGAAACACCAATAGTGATTGTGATTCCTGGCCTGACCAGTGATTCCAAAGATCCTTATATAAAGCACATCGTTTATGCTCTTGCCAAGCAAGGATGGAAAACTGTTGTTGCTAATCATCGAGGGCTGGGAGGAACTTCCATTACTTCAGATCGCTTTTACAATGCTGGGTGGACAGAAGATCTGCGGAACATTATTGCTTATGTGCATCATGAGCATCCTAAAGCGCCTTTGCTAGCAGTTGGGACAAGTATCGGTGCGAATATACTGGTGAAATATCTTGGCGAGGAGGGAGAGAATACTCCACTAGCTGGAGGTGCAGCCATATGCTGCCCTTGGGACCTTGTGATATGTGGTCGCTTTATAAAGAGGAGCTTTGTCCAAAGACTATATGACAAAATTCTGACTATTGGTCTTCGAGGTTATGCTCAGCTACATCAGAGTACTGTTAGCCGGCTTGCAGATTGGGAATGTATAAAAAAGTCACACTCTATAAGAGATTTTGACAATTATTTTACCTGCCGTATTGGTAAATTTGAGACAGTGGATACATATTATCGCAAATGCAGCAGTGCCCAATTTCTCAGTAATGTGTCAGTGCCGTTACTTTGTGTGAATGCCTTGGATGATCCTGTTTGCACAAAAGAGGCCATTCCATGGGATGAATGCAGGGCAAATAAAAATATTGTTCTTGCCACTACACTACATGGAGGACATCTTGCATTCTTTGAGGGATTAATGGCGGGTAGTATCTGGTGGGTACGGGCTGTAAAGGAATTTCTCTCTGTACTTCATTCAAGTTCTCTTATGCATACCCAGAAAAAGGTACAAACTTCTGGTCTGAGCTCAACGCTTACATCTTCTATTGACAAAGGGCCCTATTTGAATGTCTCTGAAGATGGAATGGTTAGCACCGACAATAATATTTGTATGGATTCTGAAGGGGATGACAGAGTTATGGAAGAATTACTTTTACCTTCAAAATCATCTATGAAAATATCGGGTGAAAATGAAGGTGTAGATGATGAACAACCACATGCTGCAACGGACACTCCCTTTATGCAAGAACCAAAGCTTGAAGAAGGCAGTGTAGAATATTCTCAGCTTAAGCTCTCAAATGGGGATAAAGGGTCTATATTTGAAATACGCATTAAAAGTTGTGCAGAGTTCATTGTTCTTCAATCTGTTTTACGACAACTTTTGAACCAGATGCAGGCTACTACACAAGGTAAAACTGTATTGAATGTGGGGCCAAAAAATTCAGAAACTGATGTTTTTTCTCAATTTGGAGGAAACAAGGTCATAGATGTACACAAGACTTCTGAAGATGATTTAACTTTCTGCAACTCTGATGGTTGTGCACATGTAAATGCGAAAACTGGTCCATCAAACGAACATGTAGTGCAGAAGCAAAGAACCAGCGATGACATTTCAAATAAATTAGTACCTATAAAGGCCATAATGCAACTTTCTCGGCAACAAAAGCGTTCGATGTGGATTCTGGCCTATATAGCCGTTGTAACTACATGGCCTTTAGTCGGTTCAGTTCTCGTGTTTAATTTTAAAAGAAAAGTTAAAAACATGTGGAGTGCAACATGGCATGGAAAATAG___TTGTCCCTTTAGGATTGCTCAAGTATGGTACATTGACAGATGTAACCGTCTGCTACGAGTACAGTTTCTCAATTTATGCCCATATGAATCCCTTTTCCGTTATCGTATGTTGTCATATTGTTTGGAGAACATGCCCCCATCCCTGAAATAATGAACTTCGTATATAATGATTAAATTCACTTTAATATCCTGTCATTATTTTACGTAGAATGAACACAATCTTTTTACATTAGTTCACCTTTTACTCAAGGAAGATTAAATGTCGTGTATATAAGTATATGGTTGAGCCGTTGCACGTGAGTGCATGGTTTAGCGGTCGGGGCTGATGGCTTATTCGCTCCCCACCCGCGTTCGATACCTATCGTGATGCTGGCACAACAGCCATTTGTATCATCGTGAATGTATATTGATATATGGTGTTTGATGTTGATTGATATGACAGATATCTAG

Fln Alignment:

Contig14504___LLPKCEILKGRYWSTPWLCSPHLQTAFLHFFGRPPMFQYRRQIFLPPDGGTVALDW-LTTED--IVGGAANLNTLASVHDETPIVIVIPGLTSDSKDPYIKHIVYALAKQGWKTVVANHRGLGGTSITSDRFYNAGWTEDLRNIIAYVHHEHPKAPLLAVGTSIGANILVKYLGEEGENTPLAGGAAICCPWDLVICGR-FIKRSFVQRLYDKILTIGLRGYAQLHQSTVSRLA---DWECIKKSHSIRDFDNYFTCRIGKFETVDTYYRKCSSAQFLSNVSVPLLCVNALDDPVCTKEAIPWDECRANKNIVLATTLHGGHLAFFEGLMA--GSIWWVRAVKEFLSVLHSSSL
Q40863_______________LMSKLTTLGRPYRHFPFMGNRHVETIFASFFRSWPVIKSRRECLRMEDGGTVELDWPLEGEDAELWNGELPVNS--------PVLILLPGLTGGSDDSYVKHMLLRARKHGWHSVVFNSRGCADSPVTTPQFYSASFTKDLCQVVKHVAVRFSESNIYAVGWSLGANILVRYLGEVAGNCPLSGAVSLCNPFNLVIADEDFHKGLGFNNVYDKALARGLRQIFPKHTRLFEGIEGEYNIPTVAKARSVRDFDGGLTRVSFGFQSVGDYYSNSSSSLSIKYVQTSLLCIQASNDPIAPSRGIPWEDIKENPNCLLVVTPNGGHLGWVAGDDAPFGAPWTDPLVMEYLEVLEKNQI

SNPs (tot: 41)

Position	A %	C %	G %	T %	Probability
235	33			66	0.997452
236		33	66		0.997452
1586		25		75	1.0
1803		75		25	1.0
1833	90		10		0.90298
1839	10			90	0.90298
1844	11		88		0.923564
1874			11	88	0.923564
1875	11	88			0.923564
1876		88	11		0.923564
1877			87	12	0.992554
1878		87	12		0.992554
1880		12	87		0.992554
1882			12	87	0.992554
1884		12		87	0.992554
1885	12			87	0.992554
1886	12	87			0.992554
1887			12	87	0.992554
1894		87	12		0.992554
1964	11			88	0.923564
1965	11	88			0.923564
1966		88		11	0.923564
1967	88		11		0.923564
1992	10		90		0.90298
2146		33	66		0.997452
2292	33		66		0.997452
2346	40	60			1.0
2520		16		83	0.994512
2679	16	66		16	0.988813
2680		50	33	16	1.0
2681	16	50	33		1.0
2682	16	50	16		0.988986
2683	50		16	16	0.988986
2686	40			60	1.0
2688	66			33	0.997452
2689	33		66		0.997452
2690		66		33	0.997452
2691	33	66			0.997452
2694		33	66		0.997452
2695		33	66		0.997452
2700			66	33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain