SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene13514

Length: 2037 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

UniGene Fasta

>sp_v2.0_unigene13514
CGGCAATAAAATCAATCTTCCTGGTCAGAATTATTCTGATACAAGAGTCCCACCAAATATAGGTTAATAGCACCCAATATATGGATCTTAATTCCGGGAATCAAAGTATTCCAGGACACAAGTGATGCACTGATTCAGAGGGCAGACGGTGGCCTCTCTGTTCATTCCCAACATATTCAAAGATTCGAGCTACAGCGCAGGGAACAAGGAAGTCAATAAATTTTCTGCTACTATCCGTTGCCAGGTTTGATTGAGTGTCGATATCTGCGGATGTGGGTAAAGCTTTTCTCAGGTACATATCCAATCCTTATGTTTATTGAATCCTAAGGATTGCACACGCTTCTATTGCTTGGCCTTTTGTCGAATTCAAGGATTGAACACGCTTGAATTGAAAATCCGCTCTGTGATTAATCAAAACTGCCAAACTTGAGAAATATTTTTAGGTCTACGAATATGGCAATGGCTTCTATTCTGCAATCTGTCGGGAATGAGGTTGTAGGTGGGGATCAAAATCTCTTGAGTTCTGTGGTGCCCTTATTGAAGCTCATATGCCTGAGCAGCATAGGACTGCTTCTAGCACATCCTAAGATAAACTTAATCTCCAGGGAAACATTCAAGCTTCTTAGCAAGCTTGTCTTTGCACTGTTTCTCCCATGCCTCATCTTCACAGAGCTGGGGAAATCGGTGACCTTGAAAAACATGAGCGAATGGTGGTTCATCCCTGTGAATGTGATGCTCAGCACTTGCATAGGATGTATTCTTGGGTACTTGGTAGCCCTGATCTGCAGGCCACCCCCTCAGTATTTCAGGTTCACTGTTGTCATGACTGGATTTGGGAACACAGGGAACCTCCCCCTTGCCATAATTGGATCTATATGCCACAGCTCAGACCAACCATTTGGGCAGCACTGCAATACAACAGGGGTTGCTTATATATCCTTTGCACAGTGGGTTGCAGTGATTCTGGTTTATACTTTTGTGTATCACATGCTGGAACCCCCTGAGGAGTTTTATGAGATTGTTCCCGAGGATTCCGAGATCGAAAGAGAGCCGATGGGGGATGCAAGCAGGCCTCTTCTGTTCGAAGCCGAGTGGCCTGGAATGCATCACAAGGAGACTGAGCATTGCAAGACACCATTCATTGCTCGGATCTTCAGAAGCATGTCTGGAAGTACCGAACCCAATTTAACAGAATTGGATCAGCATGTGGAGGGGTATATAGAAGGTGGGGTTAGTCCTAGGTCTGTGAGATGCTTGAATGAACCGAAAGTCGTGAGAAAGATCAGAATTGTGGCGGAGAGGACCCCGATCCAACATATTCTTCAACCTCCAACTATTGCATCTCTCTTGGCCATCATCGTGGGTATGGTTCCTCAATTGAGATCCTTCCTCTTTGATGAAGATGCTCCTCTCGCATTTTTTTACAGACAGCCTTGAGATTGTGGCAGCTGCGATGGTGCCCTCGGTGATGCTGGTTCTTGGAGGTATGCTTGCAGAAGGTCCGGATAAGTCGGAGCTTGGAATGAGAACTACTGTTGGAATTATTTTCACTAGGCTTCTGCTTCTTCCACTCGTGGGTATTGGGGTGGTGGCCTTGGCAGGGAAAATGCATATCCTTGTGAGTGGGGATAAGATGTTTTTGTTTGTGCTTCTTTTGCAGTATTCGACGCCGAGTGCAATCTTGCTTGGGGCAATAACTAGCTTGAGGGGTTATGGCACAAAGGAAACCTCTGCACTCTTGTTCTGGCAACACATTTTTGCAGTGATTTCTCTTTCATTTTATGTGACAATCTACTTCAAATTGTTTAATTATGTGTGAGGCTAGTTTACACAAATATTATGACTCAGGAACAGTATTTTCTAAGTTTCATATTTTTAAATATGGGCAATAGCTCCTTTGCAAGGGATATATGCATGATTTATTCTCTCTGTAATTCGATGAGTGTGCAAACTGCATACATGGGACCAGCTGTATTTGGTTTATGTTCTTATGGCTTGCAATTTTTTCCCTTGGGAAATTTGGTTGGTTGTGTTGCC

Ace file of the UniGene sp_v2.0_unigene13514

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	auxin efflux carrier-like protein [Arabidopsis thaliana] gb\|AAG51701.1\|AC016972_20 hypothetical protein; 37307-38680 [Arabidopsis thaliana] gb\|AAK76667.1\| unknown protein [Arabidopsis thaliana] gb\|AAL85106.1\| unknown protein [Arabidopsis thaliana] gb\|AEE3	-	-	66%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	93%
Blast2go	auxin efflux carrier family protein	-	-	81%

Source	GOs	Term	Type	Identity
Blast2go	auxin polar transport	GO:0009926	Biological Process	81%
Blast2go	transmembrane transport	GO:0055085	Biological Process	81%
Blast2go	auxin:hydrogen symporter activity	GO:0009672	Molecular Function	81%
Blast2go	integral to membrane	GO:0016021	Cellular Component	81%

Source	InterPros	Term	Type	Identity
Sma3	Multicopper oxidase, type 1	IPR001117	-	-
Sma3	Auxin efflux carrier	IPR004776	-	-
Sma3	Ribose 5-phosphate isomerase, type A	IPR004788	-	-
Sma3	Cupredoxin	IPR008972	-	-
Sma3	Multicopper oxidase, type 2	IPR011706	-	-
Sma3	Multicopper oxidase, type 3	IPR011707	-	-
Sma3	Laccase	IPR017761	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: C0PTN6

Fln msg: Overlapping hits, possible frame ERROR between 1422 and 1418,

Fln protein: MAMASILQSVGNEVVGGDQNLLSSVVPLLKLICLSSIGLLLAHPKINLISRETFKLLSKLVFALFLPCLIFTELGKSVTLKNMSEWWFIPVNVMLSTCIGCILGYLVALICRPPPQYFRFTVVMTGFGNTGNLPLAIIGSICHSSDQPFGQHCNTTGVAYISFAQWVAVILVYTFVYHMLEPPEEFYEIVPEDSEIEREPMGDASRPLLFEAEWPGMHHKETEHCKTPFIARIFRSMSGSTEPNLTELDQHVEGYIEGGVSPRSVRCLNEPKVVRKIRIVAERTPIQHILQPPTIASLLAIIVGMVPQLRSFLFDEDAPLAxxxTDSLEIVAAAMVPSVMLVLGGMLAEGPDKSELGMRTTVGIIFTRLLLLPLVGIGVVALAGKMHILVSGDKMFLFVLLLQYSTPSAILLGAITSLRGYGTKETSALLFWQHIFAVISLSFYVTIYFKLFNYV*

Protein Length: 457

Fln nts: CGGCAATAAAATCAATCTTCCTGGTCAGAATTATTCTGATACAAGAGTCCCACCAAATATAGGTTAATAGCACCCAATATATGGATCTTAATTCCGGGAATCAAAGTATTCCAGGACACAAGTGATGCACTGATTCAGAGGGCAGACGGTGGCCTCTCTGTTCATTCCCAACATATTCAAAGATTCGAGCTACAGCGCAGGGAACAAGGAAGTCAATAAATTTTCTGCTACTATCCGTTGCCAGGTTTGATTGAGTGTCGATATCTGCGGATGTGGGTAAAGCTTTTCTCAGGTACATATCCAATCCTTATGTTTATTGAATCCTAAGGATTGCACACGCTTCTATTGCTTGGCCTTTTGTCGAATTCAAGGATTGAACACGCTTGAATTGAAAATCCGCTCTGTGATTAATCAAAACTGCCAAACTTGAGAAATATTTTTAGGTCTACGAAT_-_ATGGCAATGGCTTCTATTCTGCAATCTGTCGGGAATGAGGTTGTAGGTGGGGATCAAAATCTCTTGAGTTCTGTGGTGCCCTTATTGAAGCTCATATGCCTGAGCAGCATAGGACTGCTTCTAGCACATCCTAAGATAAACTTAATCTCCAGGGAAACATTCAAGCTTCTTAGCAAGCTTGTCTTTGCACTGTTTCTCCCATGCCTCATCTTCACAGAGCTGGGGAAATCGGTGACCTTGAAAAACATGAGCGAATGGTGGTTCATCCCTGTGAATGTGATGCTCAGCACTTGCATAGGATGTATTCTTGGGTACTTGGTAGCCCTGATCTGCAGGCCACCCCCTCAGTATTTCAGGTTCACTGTTGTCATGACTGGATTTGGGAACACAGGGAACCTCCCCCTTGCCATAATTGGATCTATATGCCACAGCTCAGACCAACCATTTGGGCAGCACTGCAATACAACAGGGGTTGCTTATATATCCTTTGCACAGTGGGTTGCAGTGATTCTGGTTTATACTTTTGTGTATCACATGCTGGAACCCCCTGAGGAGTTTTATGAGATTGTTCCCGAGGATTCCGAGATCGAAAGAGAGCCGATGGGGGATGCAAGCAGGCCTCTTCTGTTCGAAGCCGAGTGGCCTGGAATGCATCACAAGGAGACTGAGCATTGCAAGACACCATTCATTGCTCGGATCTTCAGAAGCATGTCTGGAAGTACCGAACCCAATTTAACAGAATTGGATCAGCATGTGGAGGGGTATATAGAAGGTGGGGTTAGTCCTAGGTCTGTGAGATGCTTGAATGAACCGAAAGTCGTGAGAAAGATCAGAATTGTGGCGGAGAGGACCCCGATCCAACATATTCTTCAACCTCCAACTATTGCATCTCTCTTGGCCATCATCGTGGGTATGGTTCCTCAATTGAGATCCTTCCTCTTTGATGAAGATGCTCCTCTCGCAxxxxxxxxxACAGACAGCCTTGAGATTGTGGCAGCTGCGATGGTGCCCTCGGTGATGCTGGTTCTTGGAGGTATGCTTGCAGAAGGTCCGGATAAGTCGGAGCTTGGAATGAGAACTACTGTTGGAATTATTTTCACTAGGCTTCTGCTTCTTCCACTCGTGGGTATTGGGGTGGTGGCCTTGGCAGGGAAAATGCATATCCTTGTGAGTGGGGATAAGATGTTTTTGTTTGTGCTTCTTTTGCAGTATTCGACGCCGAGTGCAATCTTGCTTGGGGCAATAACTAGCTTGAGGGGTTATGGCACAAAGGAAACCTCTGCACTCTTGTTCTGGCAACACATTTTTGCAGTGATTTCTCTTTCATTTTATGTGACAATCTACTTCAAATTGTTTAATTATGTGTGA___GGCTAGTTTACACAAATATTATGACTCAGGAACAGTATTTTCTAAGTTTCATATTTTTAAATATGGGCAATAGCTCCTTTGCAAGGGATATATGCATGATTTATTCTCTCTGTAATTCGATGAGTGTGCAAACTGCATACATGGGACCAGCTGTATTTGGTTTATGTTCTTATGGCTTGCAATTTTTTCCCTTGGGAAATTTGGTTGGTTGTGTTGCC

Fln Alignment:

Contig13514___MASILQSVGNEVVGGDQNLLSSVVPLLKLICLSSIGLLLAHPKINLISRETFKLLSKLVFALFLPCLIFTELGKSVTLKNMSEWWFIPVNVMLSTCIGCILGYLVALICRPPPQYFRFTVVMTGFGNTGNLPLAIIGSICHSSDQPFGQHCNTTGVAYISFAQWVAVILVYTFVYHMLEPPEEFYEIVPEDSEIEREPMGDASRPLLFEAEWPGMHHKETEHCKTPFIARIFRSMSGSTEPNLTELDQHVEGYIEGGVSPRSVRCLNEPKVVRKIRIVAERTPIQHILQPPTIASLLAIIVGMVPQLRSFLFDEDAPLAxxTDSLEIVAAAMVPSVMLVLGGMLAEGPDKSELGMRTTVGIIFTRXXXXXXXXXXXXXXXXKMHILVSGDKMFLFVLLLQYSTPSAILLGAITSLRGYGTKETSALLFWQHIFAVISLSFYVTIYFKLFNYV
C0PTN6_______________MAYFLQSVGNEIVGGHQNLLSSVVPLLKLLCLSSIGLVLAHPKINLISKETFKLLSKLVFALFLPCLIFTELGKSVTLKNMREWWFIPANVILSTAIGCILGYLVALICRPPPQYFRFTVVMTGFGNTGNLPLAIVGSICHSSDQPFGQHCNTTGVAYISFAQWVAVILVYTFVYHMLEPPEEFYEIVPEDSEIEREPIGDASRPLLFEAEWPGMHHKETEHCKTPFIARIFRSMSGSTEPNLSELDHYVEGNIEGGGSPKSVRCLNEPKVVRKIRIVAERTPIQHILQPPTIASLLAIIVGMVPQLRSVLFDEDAPLSxxTDSLEIVAAAMVPSVMLVLGGLLAEGPDKSELGMRTTIGIIFTRLLLLPLVGIGVVALAGKLDIIVSGDKMFVFVLLLQYTTPSAILLGAMTNLRGYGTKETSALLFWQHIFAVVSLSFYVTIYFKLFNYV

SNPs (tot: 24)

Position	A %	C %	G %	T %	Probability
197		33	66		0.997452
199		66		33	0.997452
202			66	33	0.997452
208	66			33	0.997452
945		83		16	0.994512
1002	37		62		1.0
1070		90		10	0.90298
1145		10		90	0.90298
1146		90	10		0.90298
1150		10	90		0.90298
1151	10	90			0.90298
1153		90		10	0.90298
1171	12			87	0.992554
1174			87	12	0.992554
1190	88			11	0.923564
1361		87		12	0.992554
1362	87		12		0.992554
1370		12	87		0.992554
1371	12		87		0.992554
1585	10		90		0.90298
1868	14			85	0.993533
1871	83			16	0.994512
1939		20		80	0.995493
1948	80	20			0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain