SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene12996

Length: 1089 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene12996

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Cytosine-specific methyltransferase n=2 Tax=Populus trichocarpa RepID=B9INZ1_POPTR	-	-	41%
FL-Next	Isoform 3 of DNA (cytosine-5)-methyltransferase 1B OS=Oryza sativa subsp. japonica GN=MET1B	-	-	48%
Blast2go	dna methyltransferase	-	-	65%

Source	GOs	Term	Type	e value	Identity
Blast2go	methylation	GO:0032259	Biological Process	0.0	65%
Blast2go	transferase activity	GO:0016740	Molecular Function	0.0	65%

Source	InterPros	Term	Type	Identity
Sma3	Bromo adjacent homology (BAH) domain	IPR001025	-	-
Sma3	C-5 cytosine methyltransferase	IPR001525	-	-
Sma3	2Fe-2S ferredoxin, iron-sulphur binding site	IPR006058	-	-
Sma3	DNA (cytosine-5)-methyltransferase 1, eukaryote	IPR017198	-	-
Sma3	DNA methylase, C-5 cytosine-specific, active site	IPR018117	-	-

Full-Lengther Next Prediction

Fln status: Putative N-terminus

Fln database: sp_plants

Fln subject: B1Q3J6-3

Fln msg: Distance to subject end: 1180 aas, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 260 - 1486

Fln protein: MTASAQDGNENPPQRRILDFILHDEEGKSQPVEMLDVIDIYISALILPKSGDLDKEKGLRCEGFGRVESWSITGYEDGVATVWFSTGYAEYMCVKPAGCYKKFFDMFYEKAALCIEVYRALSKPDGGDPELGFDDMVARVIRSMNTKKGNNTGRFYGRDYVVSNGEFVANQLMGLDKTSSGTDQFFSGLPALVTLESECRKLDISICRDVGKDGSIKIQEGGGISAPACSSPGEDVDEDEKFARLLQEEENWNAMKTQRR

Protein Length: 261

Fln nts: TTTCACAGGAGAGAAGTTTCGGAGTTTGAAGGAGTGCATGAGATACTTGGCATCATCAGAGGCGTCCGACACGGGCTCCAAAAAACTGAAGAAAAGCGCCCCCAATCTCTCACAAAAGGGAAAACGCCCCAGAAATGAAGACGCCGACGGCGATGTCGATGATCCAACCAGCAACAGGAAGCTGCCGAAGCGCGCGGCTTCGTGCAGCGATTTCAGAGAGAAAGGTTCCTTGAGACTGAGAAAGGAAGATTGCATAATCGAAGCAAAGAAGAGAAAAGACGGTAGATGAAGAGAATGTGGGAATTCTA_-_ATGACTGCGAGTGCCCAAGATGGAAATGAAAATCCTCCGCAAAGGCGGATCTTGGATTTCATTCTCCATGATGAGGAAGGCAAGTCCCAGCCAGTCGAAATGCTGGACGTTATTGATATCTACATCTCCGCCTTAATTTTGCCCAAATCTGGAGATTTGGATAAAGAGAAGGGGCTTCGATGCGAGGGGTTTGGGCGTGTTGAGTCATGGTCCATCACAGGGTATGAGGATGGTGTGGCCACTGTGTGGTTTTCCACAGGGTATGCAGAGTATATGTGTGTGAAGCCTGCTGGATGCTACAAGAAGTTTTTTGATATGTTTTACGAGAAGGCTGCTTTGTGTATCGAGGTCTACAGAGCTTTGTCAAAACCTGATGGGGGCGATCCGGAACTGGGATTTGATGACATGGTGGCTCGAGTGATCAGGTCGATGAATACCAAGAAGGGTAATAATACTGGCCGGTTTTATGGAAGGGACTATGTCGTCTCCAATGGAGAGTTTGTTGCGAATCAGCTGATGGGTTTGGACAAAACCTCATCTGGTACTGATCAGTTTTTCTCTGGATTACCCGCGCTTGTGACCTTGGAAAGCGAGTGCAGGAAGTTGGATATAAGTATTTGTAGAGACGTGGGAAAAGACGGCAGTATAAAGATCCAGGAAGGTGGTGGTATTTCGGCACCTGCTTGTTCATCACCCGGAGAAGATGTTGACGAGGATGAGAAATTTGCTCGGTTGCTCCAGGAAGAGGAGAACTGGAACGCTATGAAGACCCAGCGAAGAG

Fln Alignment:

Contig12996___PQRRILDFILHDEEGKSQPVEMLDVIDIYISALILPKSGDL--DKEKGLRCEGFGRVESWSITGYEDGVATVWFSTGYAEYMCVKPAGCYKKFFDMFYEKAALCIEVYRALSKPDGGDPELGFDDMVARVIRSMNTKKGNNTGRFYGRDYVVSNGEFVANQLMGLDKTSSGTDQFFSGLPALVTLESECR-KLDISICRDVGKDGSIKIQEGGGISAPACSSPGEDVDEDEKFARLLQEEENWNAMK
B1Q3J6-3_____________PCRKLIDFILHDADGKLQPFEMSEIDDFFITALIMPMDDDLEKDRQKGVRCEGFGRIEDWAISGYDEGTAVVWVSTEVADYECVKPAGNYKSYYDHFYEKAQVCVEVYRKLARSVGGNPNLGLEELLASVVRSINAIKGYS--GTLSKDFVISNGEFVYNQLIGLDETANTDDEKFATLPVLLALRDGCKSRVEVSKLQPNISNGSLKINDA------ECKEVSE--DDDEKLARLLQQEEEWKMMK

SNPs (tot: 18)

Position	A %	C %	G %	T %	Probability
180	33	66			0.997452
669			16	83	0.994512
670	83			16	0.994512
672	83		16		0.994512
675		16	83		0.994512
679			20	80	0.995493
681		20		80	0.995493
682		80		20	0.995493
683	80		20		0.995493
684	80			20	0.995493
685	80	20			0.995493
690		20	80		0.995493
857	20			80	0.995493
858		20	80		0.995493
859	20		80		0.995493
861	80			20	0.995493
1047		33		66	0.997452
1079		66		33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain