SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene12824

Length: 790 nt

Origin of reads: Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene12824

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	predicted protein; K00147 glutamate-5-semialdehyde dehydrogenase [EC:1.2.1.41]	-	-	54%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	93%
Blast2go	pyrroline-5-carboxylate synthetase	-	-	82%

Source	ECs	Term	Type	e value	Identity
Blast2go	1-pyrroline-5-carboxylate dehydrogenase.	EC:1.5.1.12	-	0.0	82%

Source	KEGGs	Term	Type	Identity
Blast2go	Alanine, aspartate and glutamate metabolism	00250		82%
Blast2go	Arginine and proline metabolism	00330		82%
Blast2go	Metabolic pathways	01100		82%
Blast2go	Glutamate 5-kinase.	EC:2.7.2.11	-	82%

Source	KEGGs	Term	Type	Identity
Blast2go	Arginine and proline metabolism	00330		82%
Blast2go	Metabolic pathways	01100		82%
Blast2go	Glutamate-5-semialdehyde dehydrogenase.	EC:1.2.1.41	-	82%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Arginine and proline metabolism	00330		0.0	82%
Blast2go	Metabolic pathways	01100		0.0	82%

Source	ECs	Term	Type	e value	Identity
AutoFact	Glutamate-5-semialdehyde dehydrogenase.	EC:1.2.1.41	-	0.0	54%

Source	KEGGs	Term	Type	e value	Identity
AutoFact	Arginine and proline metabolism	00330		0.0	54%
AutoFact	Metabolic pathways	01100		0.0	54%

Source	GOs	Term	Type	Identity
Blast2go	phosphorylation	GO:0016310	Biological Process	82%
Blast2go	proline biosynthetic process	GO:0006561	Biological Process	82%
Blast2go	oxidation-reduction process	GO:0055114	Biological Process	82%
Blast2go	1-pyrroline-5-carboxylate dehydrogenase activity	GO:0003842	Molecular Function	82%
Blast2go	ATP binding	GO:0005524	Molecular Function	82%
Blast2go	glutamate 5-kinase activity	GO:0004349	Molecular Function	82%
Blast2go	glutamate-5-semialdehyde dehydrogenase activity	GO:0004350	Molecular Function	82%
Blast2go	cytoplasm	GO:0005737	Cellular Component	82%

Source	InterPros	Term	Type	Identity
Sma3	Gamma-glutamyl phosphate reductase GPR	IPR000965	-	-
Sma3	Aspartate/glutamate/uridylate kinase	IPR001048	-	-
Sma3	Glutamate/acetylglutamate kinase	IPR001057	-	-
Sma3	Glutamate 5-kinase/delta-1-pyrroline-5-carboxylate synthase	IPR005715	-	-
Sma3	Delta l-pyrroline-5-carboxylate synthetase	IPR005766	-	-
Sma3	Aldehyde dehydrogenase domain	IPR015590	-	-
Sma3	Aldehyde dehydrogenase, N-terminal	IPR016162	-	-
Sma3	Aldehyde dehydrogenase, C-terminal	IPR016163	-	-

Full-Lengther Next Prediction

Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: B8LKI9

Fln msg: Overlapping hits, possible frame ERROR between 532 and 483, Distance to subject end: 22 aas, your sequence is shorter than subject: 263 - 501

Fln protein: VVTLAVTQRDEIPDLLKLDDVIDLVIPRGSNKLVSKIRESTKIPVLSHADGVCHVYVDKAADLEMAKHIILDAKTDYPAACNAMETLLVHEELVKSGGLDELILALQTAGVKLYGGPKVTDLRNIPSANNLHYEYSSMACTLEIVKDVSAAIAHIHEHGSxxxxxxxxxxxxxxxxxxLHQLDSAAVFHNASTRFCDGARFGLGAEVGISTSRIHARGPVGVEGLLTTRWLLRGNGQVVNGDHGVKFTHKEIPVDCRKKASRI

Protein Length: 264

Fln nts: CGTCGTGACCTTGGCTGTCACTCAAAGGGACGAAATTCCAGATCTTCTTAAGCTGGATGATGTTATAGATTTAGTCATACCAAGAGGCAGCAATAAGCTGGTATCAAAAATCAGGGAATCAACGAAAATTCCTGTGCTTAGTCATGCTGATGGTGTCTGCCATGTATATGTTGATAAAGCAGCCGATTTGGAAATGGCAAAACACATCATTCTGGATGCAAAGACAGATTATCCTGCTGCTTGCAATGCCATGGAAACACTTCTTGTACACGAAGAGTTGGTGAAGAGTGGGGGGCTTGACGAGCTTATTTTGGCATTACAAACAGCAGGTGTAAAACTCTATGGTGGCCCAAAGGTCACTGATCTTCGAAATATTCCAAGTGCAAATAACCTCCATTATGAATACAGTTCCATGGCATGTACTTTGGAAATAGTGAAGGATGTTAGTGCGGCTATTGCTCATATTCATGAGCATGGAAGTxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxCTGCATCAGCTAGACAGTGCTGCAGTGTTTCATAATGCAAGTACACGATTCTGTGATGGTGCTCGCTTTGGTCTTGGTGCTGAGGTTGGAATAAGTACAAGCAGGATACATGCTCGTGGACCTGTAGGAGTTGAAGGGCTTTTAACAACCAGATGGTTGTTGAGAGGTAATGGGCAAGTGGTAAATGGTGATCATGGGGTGAAATTTACTCATAAGGAGATTCCAGTTGATTGCAGAAAGAAGGCTTCAAGAATTTT

Fln Alignment:

Contig12824___VTQRDEIPXXXXXXXXXXXXXPRGSNKLVSKIRESTKIPVLSHADGVCHVYVDKAADLEMAKHIILDAKTDYPAACNAMETLLVHEELVKSGGLDELILALQTAGVKLYGGPKVTDLRNIPSANNLHYEYSSMACTLEIVKDVSAAIAHIHEHGSAxxxxxxxxxxxxxxxxFLHQLDSAAVFHNASTRFCDGARFGLGAEVGISTSRIHARGPVGVEGLLTTRWLLRGNGQVVNGDHGVKFTHKEIPVD
B8LKI9_______________VTSRDEIPDLLKLDDVIDIVIPRGSNKLVSEIKESTKIPVLSHADGVCHVYVDKAANLDMAKQIIVDAKTDYPAACNAMETLLVHEELVKSGGLDELILALQTAGVKLYGGPKVTDLRNIPSANNLHYEYSSMACTLEIVKDVNAAIAHIHEHGSAxxxxxxxxxxxxxxxx-LHQLDSAAVFHNASTRFCDGARFGLGAEVGISTSRIHARGPVGVEGLLTTRWLLRGHGHIVDGDHGVTFNHKELPSD

SNPs (tot: 4)

Position	A %	C %	G %	T %	Probability
252		14		85	0.993533
390	66			33	0.997452
392	66	33			0.997452
393	66		33		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain