SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene12702

Length: 1560 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene12702 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Poly [ADP-ribose] polymerase 2 [Arabidopsis thaliana] sp\|Q11207.1\|PARP2_ARATH RecName: Full=Poly [ADP-ribose] polymerase 2; Short=PARP-2; AltName: Full=NAD(+) ADP-ribosyltransferase 2; Short=ADPRT-2; AltName: Full=Poly[ADP-ribose] synthase 2 emb\|CAA88288.	-	-	67%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	43%
Blast2go	poly(adp-ribose) polymerase	-	-	80%

Source	ECs	Term	Type	e value	Identity
Blast2go		EC:2.4.2.3-	-	0.0	80%

Source	GOs	Term	Type	Identity
Blast2go	protein ADP-ribosylation	GO:0006471	Biological Process	80%
Blast2go	DNA binding	GO:0003677	Molecular Function	80%
Blast2go	NAD+ ADP-ribosyltransferase activity	GO:0003950	Molecular Function	80%
Blast2go	nucleus	GO:0005634	Cellular Component	80%
Blast2go	mitochondrion	GO:0005739	Cellular Component	80%

Source	InterPros	Term	Type	Identity
Sma3	BRCT domain	IPR001357	-	-
Sma3	Zinc finger, PARP-type	IPR001510	-	-
Sma3	DNA-binding SAP	IPR003034	-	-
Sma3	Poly(ADP-ribose) polymerase, regulatory domain	IPR004102	-	-
Sma3	Exocyst complex subunit Sec15-like	IPR007225	-	-
Sma3	NAD+ ADP-ribosyltransferase	IPR008288	-	-
Sma3	WGR domain	IPR008893	-	-
Sma3	Poly(ADP-ribose) polymerase, catalytic domain	IPR012317	-	-
Sma3	PADR1	IPR012982	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: D5AAL4

Fln msg: your sequence is shorter than subject: 387 - 456

Fln protein: YTWLEMDYEGDKSCEQEQNEKKEIDTVVKPQPRQSKLDNRLAQFISLICNVSMMKQQMLEIGYNAEKLPLGKLSKSTILKGYEVLKRISIAIEQRDGDKVLGQLSGEFYTVIPHDFGFKKMRDFIINTPQKLKRKLEMVEALGEIELAAKLLKDDTEEEEDPLYSHYKRLRCNILPLDGDSEDVMLIKRYLSNTHAETHSSYTIEVLQVFKVSRDDEDDHFQKFRTTENRMLLWHGSRLSNWTGILSQGLRIAPPEAPVTGYMFGKGVYFADMFSKSANYCFASPSSPVGVLLLCEVALGDMVELLEANYHADNLPAGKLSTKGVGVTAPDPSEFCTLPDGVVVPLGTPKKRSATRGSLLYNEFIVYNVEQIRMRYVLQVKFIFKR*

Protein Length: 388

Fln nts: GTATACTTGGTTAGAAATGGATTATGAGGGAGATAAATCATGTGAACAAGAACAAAATGAAAAGAAGGAAATTGACACCGTTGTTAAGCCTCAACCTCGACAAAGTAAGTTGGACAATCGCCTAGCACAATTTATCTCTCTCATATGCAATGTGAGCATGATGAAACAGCAAATGCTGGAGATTGGCTATAATGCTGAGAAGCTACCTCTTGGAAAGTTGAGCAAGTCAACTATCTTAAAGGGTTACGAAGTGTTGAAAAGGATTAGCATTGCAATTGAACAGCGAGATGGAGACAAAGTACTTGGGCAACTGAGCGGTGAGTTCTACACAGTGATTCCGCATGATTTTGGCTTCAAAAAAATGCGTGATTTCATCATCAACACACCACAAAAACTGAAGAGGAAGCTAGAAATGGTTGAGGCATTGGGTGAAATTGAACTTGCAGCAAAGTTATTGAAGGATGATACTGAAGAAGAGGAAGATCCTCTATATTCTCATTATAAACGACTTCGTTGCAATATTCTTCCGTTGGATGGAGATTCTGAAGATGTGATGTTGATAAAACGATACTTGAGTAATACCCATGCTGAGACACATTCAAGCTACACAATTGAAGTTTTACAAGTTTTCAAAGTTTCAAGAGATGATGAAGATGATCACTTTCAAAAATTTCGCACTACAGAAAATCGGATGCTTTTATGGCATGGTTCAAGACTATCAAATTGGACAGGCATTCTTTCACAAGGACTCAGAATTGCTCCTCCAGAAGCACCAGTTACTGGGTACATGTTTGGAAAGGGAGTGTATTTTGCTGATATGTTTTCCAAAAGTGCAAACTACTGCTTTGCATCTCCTTCATCTCCAGTCGGTGTACTTCTCTTGTGTGAGGTGGCATTAGGTGACATGGTTGAACTTCTAGAAGCAAATTATCATGCAGACAACTTGCCAGCTGGAAAGCTAAGCACAAAAGGAGTTGGTGTAACAGCACCTGATCCATCAGAGTTCTGCACTCTTCCTGATGGAGTTGTTGTTCCATTGGGAACACCAAAGAAGCGATCTGCTACACGTGGTTCTCTTTTGTACAATGAGTTTATTGTTTACAACGTGGAACAAATACGCATGCGGTACGTGCTCCAGGTGAAATTTATCTTTAAACGATGA___AGAAAGAAAGCGAATGTCTATGGCCTCATATAGACTTCGGTGATAGCTGCTGCTAGCAATCACACAAAATTCCGTAGTCATAAATGCTGAGAGAGAGAGAAAGAGCCAGCCAGCCATGTTTCTTTTTGATAACAGAGAGAGCGGTGGGGTAGGTGGAGGAAAGCGCAATCTGCATTGGACCATTTTAAACATTAACATACAGTTTTTCTATATTTAATGATCAAATATTGACATATTGTGAAGTTATTTTATTAGGTTAAATTATCAATTATCATCTATTGAAGCCTAGTAGGAAATATGTTTGCGCCTGCTGATTTTGGAAGTAGTTGAAGCATATCATGACTGAAAACATGTAAGGAACAACAAAGAAATACTAAATAAGGCCATGGACAACTACA

Fln Alignment:

Contig12702___LEMDYEGDKSCEQEQNEKKEIDTVVKPQPRQSKLDNRLAQFISLICNVSMMKQQMLEIGYNAEKLPLGKLSKSTILKGYEVLKRISIAIEQRDGDKVLGQ-----LSGEFYTVIPHDFGFKKMRDFIINTPQKLKRKLEMVEALGEIELAAKLLKXXXXXXXDPLYSHYKRLRCNILPLDGDSEDVMLIKRYLSNTHAETHSSYTIEVLQVFKVSRDDEDDHFQKFRTT-ENRMLLWHGSRLSNWTGILSQGLRIAPPEAPVTGYMFGKGVYFADMFSKSANYCFASPSSPVGVLLLCEVALGDMVELLEANYHADNLPAGKLSTKGVGVTAPDPSEFCTLPDGVVVPLGTPKKRSATRGSLLYNEFIVYNVEQIRMRYVLQVKFIFK
D5AAL4_______________LEIDYGVDES------EQKDIGSL----GAKSKLDPRVIDLMKMLFDLETYRAAMMEFEINMSEMPLGKLSKSNIQRGFEVLTEVQNLIKQNEPGVALNESLIIDASNRFFTLIP------SVHPHIIRDEDDLKSKIQMLETLRDIEIASSLINFKKNDED-PFDSNYKKLQCNISPLPHDSSDYQLIKKYLETTHAPTHKEWALELEDAYAIDRKGEYDAYFPYKSNFKNRMLLWHGSRVTNFVGILSQGLRIAPPEAPATGYMFGKGLYFADLVSKSAQYCYTHKKNPVGLMLLSEVALGEVHELKSAKY-MDKPPKGKHSTKGVGKTKPLDTEYERWGDDVTVPCGRPVPSSVKNSDLLYNEYIVYDPAQVKLQFLLKVRFRYK

SNPs (tot: 56)

Position	A %	C %	G %	T %	Probability
37		20		80	0.995493
113	16		83		0.994512
163	16		83		0.994512
178	16		83		0.994512
187		16		83	0.994512
230	50		50		1.0
232			16	83	0.994512
233	16		83		0.994512
237		16		83	0.994512
238	16			83	0.994512
239	16			83	0.994512
240		66	33		1.0
241		83	16		0.994512
247		40		40	1.0
248			20	80	0.995493
251		80	20		0.995493
259		50		50	1.0
291		33		66	0.997452
325	33			66	0.997452
329	33		66		0.997452
352	33			66	0.997452
360		33		66	0.997452
378		33	66		0.997452
413	66			33	0.997452
487	37			62	1.0
493	50			50	1.0
497	10		90		0.90298
502	90		10		0.90298
506		50		50	1.0
521		90		10	0.90298
522		90	10		0.90298
559		57		42	1.0
635	16			83	0.994512
661	83	16			0.994512
697	20		40	40	1.0
770	80	20			0.995493
771	80	20			0.995493
887		50		50	1.0
900	80			20	0.995493
992	20			80	0.995493
1008		40		60	1.0
1014		83	16		0.994512
1056	16		83		0.994512
1057		16		83	0.994512
1066	80		20		0.995493
1094	20		80		0.995493
1096	80		20		0.995493
1136	80		20		0.995493
1144	80	20			0.995493
1153	25			75	0.996473
1157		75		25	0.996473
1435	12			87	0.992554
1436		12	87		0.992554
1438			87	12	0.992554
1484		80		20	0.995493
1495		20		80	0.995493

SSRs (tot: 2)

Start position	End position	Sequence	Length
283	295	TGGC TGGC TGGC T	13
299	310	TC TC TC TC TC TC	12

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain