SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene12632

Length: 1500 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene12632 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Peptidase M28 family protein [Arabidopsis thaliana] gb\|AAP37682.1\| At5g19740 [Arabidopsis thaliana] gb\|AED92744.1\| Peptidase M28 family protein [Arabidopsis thaliana]	-	-	56%
FL-Next	sp=Probable glutamate carboxypeptidase 2; Arabidopsis thaliana (Mouse-ear cress).	-	-	44%
Blast2go	peptidase m28 family protein	-	-	68%

Source	ECs	Term	Type	e value	Identity
Blast2go	Hydrolases, Acting on peptide bonds (peptide hydrolases), Dipeptidases.	EC:3.4.13.-	-	0.0	68%

Source	GOs	Term	Type	Identity
Blast2go	proteolysis	GO:0006508	Biological Process	68%
Blast2go	dipeptidase activity	GO:0016805	Molecular Function	68%
Blast2go	vacuole	GO:0005773	Cellular Component	68%

Source	InterPros	Term	Type	Identity
Sma3	Protease-associated domain, PA	IPR003137	-	-
Sma3	Transferrin receptor-like, dimerisation domain	IPR007365	-	-
Sma3	Peptidase M28	IPR007484	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: sp_plants

Fln subject: Q9M1S8

Fln msg: your sequence is shorter than subject: 368 - 705

Fln protein: RRDLGCHSSWTFGACDPNSGTATLLEISQRLGKLLKQGWQPKRSILLCNWDAEEYALIGSTEWVEQNYDLLFSSAVAYLNVDEAVKGPGFKLQSTPQLDDFIQDIAKEVEDPDNPGKTIYESWASSSQDSRPPIKRLGGGGSDYAPFVQHAGIPGTDFTFGGDFPAYHSLYDDYMWMAKYGDPHFHRHVALGAMWGLTALKLADAKVLPFNYTTYADNLQLYVDTLESQLSSANAPSFVSTNPLRKSIAELRKAALQIKKKAQELEALEDGSADLRSLNDHLVMAERAFTDADGLPGSPWYKHLVYGPSKINKYGTTAFPGISDLLALVAGDEANHGGDGWAGVQHQIWRVSRVISRVALVIHGELS*

Protein Length: 369

Fln nts: CGTCGTGACCTTGGCTGTCACTCATCATGGACTTTTGGTGCCTGTGACCCGAATAGCGGAACTGCAACCCTTCTGGAAATATCTCAGAGACTAGGGAAGTTGCTAAAACAGGGTTGGCAGCCAAAGAGAAGTATACTATTGTGTAACTGGGATGCAGAGGAATACGCATTGATTGGATCCACGGAATGGGTGGAGCAGAACTATGATTTACTGTTCTCATCAGCCGTGGCATATCTCAATGTTGATGAGGCTGTCAAGGGCCCGGGGTTTAAGCTTCAATCAACACCCCAACTTGACGATTTTATTCAGGATATAGCTAAAGAGGTGGAAGATCCAGACAATCCTGGAAAGACAATCTATGAATCATGGGCTTCTTCCAGCCAAGATTCCCGTCCTCCTATTAAAAGGTTAGGTGGAGGGGGATCCGATTATGCCCCATTTGTGCAGCATGCCGGAATTCCAGGCACTGACTTCACCTTTGGAGGAGACTTTCCGGCATATCATTCTCTCTATGATGATTACATGTGGATGGCAAAATATGGCGACCCGCATTTTCACAGGCATGTTGCACTGGGAGCAATGTGGGGTCTAACAGCGCTCAAGCTAGCGGATGCAAAGGTTCTCCCTTTCAACTATACAACGTATGCCGACAATCTGCAGTTGTATGTGGATACATTAGAATCACAGTTAAGCTCTGCCAATGCACCTTCCTTCGTGTCTACAAATCCATTGCGTAAGTCCATTGCTGAGCTCAGAAAAGCTGCGTTGCAGATTAAGAAAAAAGCACAGGAACTGGAGGCTCTTGAGGATGGAAGTGCAGATCTGAGGAGCTTGAATGACCATCTTGTAATGGCAGAGCGTGCATTTACAGACGCAGATGGGCTGCCTGGGTCGCCTTGGTATAAGCACTTGGTATATGGGCCGTCTAAAATCAACAAGTATGGGACAACAGCGTTTCCTGGTATAAGTGACTTGCTTGCGCTTGTAGCAGGTGATGAGGCCAATCACGGTGGTGATGGGTGGGCTGGTGTACAGCACCAAATATGGAGGGTGTCAAGAGTAATCAGCCGTGTAGCTCTGGTCATTCATGGGGAGCTCAGTTGA___TTTGGTCATTTTCAAGCTGTTATGAGTTGGGTCTCTATTCAAAATGTTCCCAGGGGGGTAAACAAAGGCAATGGCACATGAGATGCCATAAGGCCAGTGGGATTGATACCATAAGGCCCATCATCATGTTAGCTTAGGAACCAACAAAATTAGAGTAAAACTACGTATCTCTGGTTTTCGATAATATCATCAGGTTAGATGGGGCACTGGGGCAAACAAGATCAGAGTAAAACTAGTATGGTTTAGGACGTCATAAACAAGAAAGCTATTAAAATTGTAATCAGCATATTATAAGGTTACAATAATTAGAATCAAGCTAACGATCTTGTGTTGTCATTTTGTAATATCCCAGCATTATGATCGTCTTGTCATTTTGTAATATCCCAGCATTACATT

Fln Alignment:

Contig12632___LGCH-SSWTFGACDPNSGTATLLEISQRLGKLLKQGWQPKRSILLCNWDAEEYALIGSTEWVEQNYDLLFSSAVAYLNVDEAVKGPGFKLQSTPQLDDFIQDIAKEVEDPDNPGKTIYESWASSSQDSRPPIKRLGGGGSDYAPFVQHAGIPGTDFTFGGDFPAYHSLYDDYMWMAKYGDPHFHRHVALGAMWGLTALKLADAKVLPFNYTTYADNLQLYVDTLESQLSSANAPSFVSTNPLRKSIAELRKAALQIKKKAQELEALEDGSADL------RSLNDHLVMAERAFTDADGLPGSPWYKHLVYGPSKINKYGTTAFPGISDLLALVAGDEANHGGDGWAGVQHQIWRVSRVISRVALVIHG
Q9M1S8_______________LGNHRDAWTYGAVDPNSGTSALLDISRRFALLLKSGWRPRRTILLCSWDAEEFGMIGSTEWIEENVLNLGASAVAYLNVDCAVQGSGFFAGATPQLDGLLVDVLKLVQDPDAVGLTVEETFKSQNN----IIQRLSRVDSDFSGFLHHAGIPSIDMYYGADYPVYHTAFDSYDWMIHNADPLFHRHVAMAGIWGLLGILLADEPLIPFDYISYADQLQAHRDKLSKLLEGK-----VSVNPLSMAIQEFSLVAKEAADEAKKLKGKSYSKNDVAAAAKRRELNDRLMLVERGFLDAEGIKGKEWFKHLVYGPAAEPESKLGFFPGIADAIAMNASEGI---------IEHEIWRVARAIQRASKALKG

SNPs (tot: 4)

Position	A %	C %	G %	T %	Probability
151	25	75			0.996473
1320	25	75			0.996473
1321	25		75		0.996473
1423		16		83	0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain