SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene12384

Length: 833 nt

Origin of reads: Biogeco1 v2 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene12384 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Beta-glucosidase, GBA2 type family protein [Arabidopsis thaliana] ref\|NP_001030757.1\| Beta-glucosidase, GBA2 type family protein [Arabidopsis thaliana] gb\|AAL38843.1\| unknown protein [Arabidopsis thaliana] dbj\|BAE99039.1\| hypothetical protein [Arabidopsis	-	-	58%
FL-Next	sp=Non-lysosomal glucosylceramidase; Physcomitrella patens subsp. patens (Moss).	-	-	62%
Blast2go	non-lysosomal glucosylceramidase-like	-	-	73%

Source	GOs	Term	Type	Identity
Blast2go	sphingolipid metabolic process	GO:0006665	Biological Process	73%
Blast2go	hydrolase activity, acting on glycosyl bonds	GO:0016798	Molecular Function	73%
Blast2go	plasma membrane	GO:0005886	Cellular Component	73%

Source	InterPros	Term	Type	Identity
Sma3	Phosphotransferase system, HPr serine phosphorylation site	IPR002114	-	-
Sma3	Glucosylceramidase	IPR006775	-	-
Sma3	Beta-glucosidase, GBA2 type	IPR014551	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: tr_plants

Fln subject: A9S119

Fln msg: Distance to subject end: 686 aas, atg_distance in limit (1-15): atg_distance = 4, your sequence is shorter than subject: 264 - 970

Fln protein: MAYGESMNSKKDPSSEDHAQKTTATKEQHDQGLSQQLAWHRKLNSHSNILTEFTLTLMEKFKLAGLGLRMWRYVQEEISRGRRPIINPFNSEAKDSCHGVPLGGMGAGSIGRGYRGDFRRWQLIPGSCDDAPIIANQFSVFVSRQGEKNYASVLFPGCPKELKQNQNSGILSWDWNLDGQHSTYHALFPRAWTVYDGEPDPELKITSRQISPFIPHNYKESSLPVAVFSYTLKNRGKTTANVSLLFTWTNSIGGKSEFSGGHVN

Protein Length: 265

Fln nts: CGTCGTGACCTTGGCTGTCACTCATCTCAAAGATATCGGG_-_ATGGCTTACGGAGAATCAATGAATAGCAAAAAGGATCCTTCTTCCGAAGATCATGCGCAGAAAACAACCGCTACTAAGGAGCAGCATGATCAGGGGCTTTCTCAACAACTTGCATGGCATCGTAAACTAAACAGTCATTCTAATATTCTTACTGAATTTACTTTGACTCTGATGGAGAAATTCAAGCTGGCTGGCTTAGGTTTGCGAATGTGGCGTTATGTTCAAGAAGAAATTTCTCGAGGAAGGAGGCCAATAATTAATCCCTTCAATAGTGAGGCAAAGGATTCCTGTCACGGTGTTCCACTTGGAGGGATGGGTGCGGGGAGCATAGGAAGAGGTTATCGAGGTGACTTTAGGCGTTGGCAGCTGATACCAGGCAGTTGCGATGACGCTCCTATCATAGCAAACCAGTTTTCAGTATTTGTTTCTCGTCAGGGGGAGAAAAACTATGCATCGGTGTTATTTCCTGGATGCCCCAAAGAACTCAAGCAAAATCAAAATTCGGGAATATTGTCTTGGGACTGGAACTTAGATGGACAACATTCTACTTACCATGCTCTCTTCCCACGGGCATGGACAGTATATGATGGTGAGCCAGACCCTGAGCTAAAAATCACCTCCCGACAGATTTCTCCTTTTATTCCTCACAACTACAAAGAGAGTAGTTTACCAGTAGCTGTATTCTCTTACACGCTAAAAAATAGAGGGAAAACCACTGCAAATGTCAGCTTGCTTTTCACTTGGACTAATTCAATTGGTGGAAAGTCAGAGTTTTCTGGTGGTCATGTCAATT

Fln Alignment:

Contig12384___DHAQKTTATKEQHDQGLSQQLAWHRKLNSHSNILTEFTLTLMEKFKLAGLGLRMWRYVQEEISRGRRPIINPFNSEAKDS-CHGVPLGGMGAGSIGRGYRGDFRRWQLIPGSCDDAPIIANQFSVFVSRQG-EKNYASVLFPGCPKELK--QNQNSGILSWDWNLDGQHSTYHALFPRAWTVYDGEPDPELKITSRQISPFIPHNYKESSLPVAVFSYTLKNRGKTTANVSLLFTWTNSIGGKSEFSGGHVN
A9S119_______________DYVQKPTLALLEDDPGAPPHQAWRRKLNSHAEVLKEFRVTFMEGFKMLRLGMRMWTYVQSEKSEGRLPPIDPFRRETKPSACHGVPCGGMGGGSIGRGFRGEFRRWQIVPGVCDEAPVLANQFSVHVSLSGGEKAVSSVLYPGRPRELKGLKNDENGISSWDWKLDGQNTTYHALFPRAWTIYEGEPDPALKISCRQISPFIPHNYKESSYPACVFSYVAVNTGKEVADVNLLFTWANSIGSTTGSTGGHIN

SNPs (tot: 13)

Position	A %	C %	G %	T %	Probability
385			75	25	0.996473
387	25			75	0.996473
388			25	75	0.996473
391	25	75			0.996473
392		25		75	0.996473
394			25	75	0.996473
395		25	75		0.996473
396	75	25			0.996473
397	25			75	0.996473
401	75			25	0.996473
402		25	75		0.996473
406	25			75	0.996473
408	75		25		0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain