SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene12170

Length: 2237 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene12170
AATGTCAAAAGAGAGCAGGACATTGACATCGGGGAGTGTAGAAACAACCAGAGCATGGTTGAGTTGGAGCATACAGAATTTCTGTCTTATCCTCCAGAGTCTGATGACTTCTATGGAGGGCTTTCAGACTGCCAGCAAAACTGTCTCAAGAGTGAGAGTTGCATTGCAGCCACAGTCTTAAACGATGGTTCAGGAATGTGCCGCCAAAAGACAACAAATTTTACTAGTGCATACCAATCCATAACAATTCCAAGCACATCTTATATTAAGGTATGCGGTGAGCAACGCTTTCTGCCTCCGCCTCCAGTGATGCCAGCAACCACATCTTCGAGGACAGTCAAAGTATCTGTCGCTGGAATTGCAGTAGCTGTTATGGTTACTCTGCTTGCCCTTGTAATCATTGAGGCAGGATTTTGGTGGTTTTTCTGCAGAAACAATCCTAGCTTTGGAGGATTGTCGGCTCAATACACACTTTTAGAGTATGCTTCTGGAGCTCCAGTTCAGTTTTCTTACAAAGATCTTCAGCAATCAACGAGGAATTTTAAAGAGAAGGTGGGTTCAGGTGGCTTTGGGACAGTATACAAGGGTTCATTTCATAACAGGAGCTTGATTGCAGTTAAGAAGCTGGAAGGAATCGAGCAAGGAGACAAGCAGTTTCGCATGGAAGTAGCAACAATAAGCAGCACTCATCACCTAAACCTGGTTAGGCTAATTGGGTTTTGCTCAGAGGGGCGGCATAGGTTGTTGGTATATGAATTCATGAAAAATACTTCTTTGGATACTTTTCTTTTTGAATCTTCAGAGCAAACCAGGAAGTTAGATTGGCAGACAAGGCTCAATATAGCTCTTGGCACTGCAAGAGGAATTGCCTATTTGCATGAAGAATGTAGGGATTGCATAATTCATTGTGATATCAAACCAGAGAACATACTTCTAGATGATAATTTGAATGCCAAGGTCTCTGATTTTGGGCTTGCAAAACTCATTAATACAAGGGATCATCGAAACCATACATTGACTAGTGTTAGAGGGACTAGAGGGTACTTAGCTCCAGAGTGGCTTGCTAATTTGCCAATTACTTCCAAATCTGATGTTTTCAGTTATGGTATGGTTTTATTGGAGATCATAAGTGGTAGAAGGAACTTTGATATCTCTGCTTCCTCAGGGAAGAGGAAATTCTCCACATGGGCATATGAGGAATTTGAAAAGGGAAATATTTTTAGCATTGTAGATAAGAGGCTTGGTGAGGACATACATATGGATGAGGTGGAGAGGGCAATTCAAGTTAGTTTTTGGTGCATCCAAGAGCAACCTTCTCAGCGACCTTCTATGGGGAAGGTTGTGAAAATGCTAGAGGGGGTTTTACCTGTCAACAAACCACCCACTCCAAGATCTTTTGAAGGACTTCAAAGCAGTGCTAGTATCAATGCCAGTGTTAGTGCCAGTGGTAGCATGATCTCAGTGTTTGCTGCTTCTGCTCCTCCAGCATCTTCCTCCTCTTCTGCAGATGTATTCATGTCCCCAACTTCAGGACATATCATGGAAATGGCATTTTCCTCAGGAAATTTGCCATCTTCTGACAACAATATACCTGTTGAGCATGTCAAACCCCTTCCTCCTACCCCCCAAAACTAGGTAGTTTCTATTGCAATTCAGAAGGGTACTGGGTTTTCTTTCTATATCCTGAAAATGTCCTCAATTCAGTGTTTTCATCTTCTAATTTTTGTGTTTAGCTGCAAATGTCATTGTATCAATGGACCTCAATGAAGACAGGACCTGGATTCTGAAGGTTTTGCTGCCTCATGGATTTCAGGACTGCATAAAAGTTTCTTGGTTCATGCAAATCTGCAAGCAAGTCAACTTAAAGCTGTAAGGAGATTTGTTCATTATATCAAGGGCACTTACCAATTCAGTATAAATTATTACAATGGCAAAGTGACTTAGTTTGTTGGATGGATAGGGCTAGAGATGACCATGGCAAGAAGTCAAATTCCAGCTGTGTTTTGACTTGGCTTTGAACCCATTGTCGTCTTCTTAAAAGTAGGAGGTGGTTTCAATGTTGACTACAAAAGTAAATATTGTGGTACAGTGCATGTTGGCACATAGGCTTTGTGGATTCAACATCTCATGGGCAAACACAGTTTTGTAGTTGAGTTATTGACAGTGGTATATTGTGACAATCAAAGTGCAATACAGGTTACAAAAATTCCCGTTGCACATAGCAAAATGAAGACATT

Ace file of the UniGene sp_v2.0_unigene12170 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	lectin protein kinase-like protein [Arabidopsis thaliana] sp\|Q9XID3.1\|Y1343_ARATH RecName: Full=G-type lectin S-receptor-like serine/threonine-protein kinase At1g34300; Flags: Precursor gb\|AAD39605.1\|AC007454_4 Contains similarity to gi\|479356 protein kin	-	-	58%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	50%
Blast2go	protein	-	-	80%

Source	ECs	Term	Type	e value	Identity
Blast2go	Transferases, Transferring phosphorous-containing groups, Protein-serine/threonine kinases.	EC:2.7.11.-	-	0.0	80%

Source	GOs	Term	Type	Identity
Blast2go	recognition of pollen	GO:0048544	Biological Process	80%
Blast2go	protein phosphorylation	GO:0006468	Biological Process	80%
Blast2go	sugar binding	GO:0005529	Molecular Function	80%
Blast2go	protein binding	GO:0005515	Molecular Function	80%
Blast2go	ATP binding	GO:0005524	Molecular Function	80%
Blast2go	protein serine/threonine kinase activity	GO:0004674	Molecular Function	80%
Blast2go	plasma membrane	GO:0005886	Cellular Component	80%

Source	InterPros	Term	Type	Identity
Sma3	GPCR, rhodopsin-like, 7TM	IPR000276	-	-
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Epidermal growth factor-like domain	IPR000742	-	-
Sma3	S-locus glycoprotein	IPR000858	-	-
Sma3	Phospholipase C, phosphatidylinositol-specific , X domain	IPR000909	-	-
Sma3	Glycosyl transferase, family 51	IPR001264	-	-
Sma3	Bulb-type lectin domain	IPR001480	-	-
Sma3	Leucine-rich repeat	IPR001611	-	-
Sma3		IPR001899	-	-
Sma3	PAN-1 domain	IPR003014	-	-
Sma3	Long hematopoietin receptor, Gp130 family 2, conserved site	IPR003529	-	-
Sma3	Apple-like	IPR003609	-	-
Sma3	Epidermal growth factor-like	IPR006210	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	EGF-like region, conserved site	IPR013032	-	-
Sma3	PAN-2 domain	IPR013227	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-
Sma3	Peptidase S26A, signal peptidase I, serine active site	IPR019756	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NM60

Fln msg: your sequence is longer than subject: 527 - 431

Fln protein: MVELEHTEFLSYPPESDDFYGGLSDCQQNCLKSESCIAATVLNDGSGMCRQKTTNFTSAYQSITIPSTSYIKVCGEQRFLPPPPVMPATTSSRTVKVSVAGIAVAVMVTLLALVIIEAGFWWFFCRNNPSFGGLSAQYTLLEYASGAPVQFSYKDLQQSTRNFKEKVGSGGFGTVYKGSFHNRSLIAVKKLEGIEQGDKQFRMEVATISSTHHLNLVRLIGFCSEGRHRLLVYEFMKNTSLDTFLFESSEQTRKLDWQTRLNIALGTARGIAYLHEECRDCIIHCDIKPENILLDDNLNAKVSDFGLAKLINTRDHRNHTLTSVRGTRGYLAPEWLANLPITSKSDVFSYGMVLLEIISGRRNFDISASSGKRKFSTWAYEEFEKGNIFSIVDKRLGEDIHMDEVERAIQVSFWCIQEQPSQRPSMGKVVKMLEGVLPVNKPPTPRSFEGLQSSASINASVSASGSMISVFAASAPPASSSSSADVFMSPTSGHIMEMAFSSGNLPSSDNNIPVEHVKPLPPTPQN*

Protein Length: 528

Fln nts: AATGTCAAAAGAGAGCAGGACATTGACATCGGGGAGTGTAGAAACAACCAGAGC_-_ATGGTTGAGTTGGAGCATACAGAATTTCTGTCTTATCCTCCAGAGTCTGATGACTTCTATGGAGGGCTTTCAGACTGCCAGCAAAACTGTCTCAAGAGTGAGAGTTGCATTGCAGCCACAGTCTTAAACGATGGTTCAGGAATGTGCCGCCAAAAGACAACAAATTTTACTAGTGCATACCAATCCATAACAATTCCAAGCACATCTTATATTAAGGTATGCGGTGAGCAACGCTTTCTGCCTCCGCCTCCAGTGATGCCAGCAACCACATCTTCGAGGACAGTCAAAGTATCTGTCGCTGGAATTGCAGTAGCTGTTATGGTTACTCTGCTTGCCCTTGTAATCATTGAGGCAGGATTTTGGTGGTTTTTCTGCAGAAACAATCCTAGCTTTGGAGGATTGTCGGCTCAATACACACTTTTAGAGTATGCTTCTGGAGCTCCAGTTCAGTTTTCTTACAAAGATCTTCAGCAATCAACGAGGAATTTTAAAGAGAAGGTGGGTTCAGGTGGCTTTGGGACAGTATACAAGGGTTCATTTCATAACAGGAGCTTGATTGCAGTTAAGAAGCTGGAAGGAATCGAGCAAGGAGACAAGCAGTTTCGCATGGAAGTAGCAACAATAAGCAGCACTCATCACCTAAACCTGGTTAGGCTAATTGGGTTTTGCTCAGAGGGGCGGCATAGGTTGTTGGTATATGAATTCATGAAAAATACTTCTTTGGATACTTTTCTTTTTGAATCTTCAGAGCAAACCAGGAAGTTAGATTGGCAGACAAGGCTCAATATAGCTCTTGGCACTGCAAGAGGAATTGCCTATTTGCATGAAGAATGTAGGGATTGCATAATTCATTGTGATATCAAACCAGAGAACATACTTCTAGATGATAATTTGAATGCCAAGGTCTCTGATTTTGGGCTTGCAAAACTCATTAATACAAGGGATCATCGAAACCATACATTGACTAGTGTTAGAGGGACTAGAGGGTACTTAGCTCCAGAGTGGCTTGCTAATTTGCCAATTACTTCCAAATCTGATGTTTTCAGTTATGGTATGGTTTTATTGGAGATCATAAGTGGTAGAAGGAACTTTGATATCTCTGCTTCCTCAGGGAAGAGGAAATTCTCCACATGGGCATATGAGGAATTTGAAAAGGGAAATATTTTTAGCATTGTAGATAAGAGGCTTGGTGAGGACATACATATGGATGAGGTGGAGAGGGCAATTCAAGTTAGTTTTTGGTGCATCCAAGAGCAACCTTCTCAGCGACCTTCTATGGGGAAGGTTGTGAAAATGCTAGAGGGGGTTTTACCTGTCAACAAACCACCCACTCCAAGATCTTTTGAAGGACTTCAAAGCAGTGCTAGTATCAATGCCAGTGTTAGTGCCAGTGGTAGCATGATCTCAGTGTTTGCTGCTTCTGCTCCTCCAGCATCTTCCTCCTCTTCTGCAGATGTATTCATGTCCCCAACTTCAGGACATATCATGGAAATGGCATTTTCCTCAGGAAATTTGCCATCTTCTGACAACAATATACCTGTTGAGCATGTCAAACCCCTTCCTCCTACCCCCCAAAACTAG___GTAGTTTCTATTGCAATTCAGAAGGGTACTGGGTTTTCTTTCTATATCCTGAAAATGTCCTCAATTCAGTGTTTTCATCTTCTAATTTTTGTGTTTAGCTGCAAATGTCATTGTATCAATGGACCTCAATGAAGACAGGACCTGGATTCTGAAGGTTTTGCTGCCTCATGGATTTCAGGACTGCATAAAAGTTTCTTGGTTCATGCAAATCTGCAAGCAAGTCAACTTAAAGCTGTAAGGAGATTTGTTCATTATATCAAGGGCACTTACCAATTCAGTATAAATTATTACAATGGCAAAGTGACTTAGTTTGTTGGATGGATAGGGCTAGAGATGACCATGGCAAGAAGTCAAATTCCAGCTGTGTTTTGACTTGGCTTTGAACCCATTGTCGTCTTCTTAAAAGTAGGAGGTGGTTTCAATGTTGACTACAAAAGTAAATATTGTGGTACAGTGCATGTTGGCACATAGGCTTTGTGGATTCAACATCTCATGGGCAAACACAGTTTTGTAGTTGAGTTATTGACAGTGGTATATTGTGACAATCAAAGTGCAATACAGGTTACAAAAATTCCCGTTGCACATAGCAAAATGAAGACATT

Fln Alignment:

Contig12170___LEYASGAPVQFSYKDLQQSTRNFKEKVGSGGFGTVYKGSFHNRSLIAVKKLEG-IEQGDKQFRMEVATISSTHHLNLVRLIGFCSEGRHRLLVYEFMKNTSLDTFLFESSEQTRKLDWQTRLNIALGTARGIAYLHEECRDCIIHCDIKPENILLDDNLNAKVSDFGLAKLINTRDHRNHTLTSVRGTRGYLAPEWLANLPITSKSDVFSYGMVLLEIISGRRNFDISASSGKRKFSTWAYEEFEKGNIFSIVDKRL----GEDIHMDEVERAIQVSFWCIQEQPSQRPSMGKVVKMLEGVLPVNKPPT
A9NM60_______________LKNLTGLPAIFTYETIEIATGGFSKEIGKGGFGTVYEGILEDDTLVAVKCLVNESRQGQAEFCAEIGTTSSINHSNLVRLHGICVEGQHRILVYEFMANGSLDRWLFDSDKW---LDWKTRYSIALDTARGLAYLHEESRLCILHLDVKPQNILVDEYFKAKVSDFGMARCLK-RDIESHLVTGVRGTPGYMAPEWLLGAGITSKSDVFSYGMVLLEIISGRRNVDNTRDSDNWYFPSIAINKARQDKMEEIIETGLEMKRPED--LEEAYRLIKTALWCVQSNSGLRPSMGTVVRILEGDLEILDPPS

SNPs (tot: 10)

Position	A %	C %	G %	T %	Probability
482		80	20		0.995493
484			20	80	0.995493
488		25		75	0.996473
493			25	75	0.996473
495	75			25	0.996473
498			25	75	0.996473
499	25			75	0.996473
500		25		75	0.996473
1458	80		20		0.995493
1628		40		60	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain