SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene12087

Length: 2249 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene12087
AGAAATATTTAGCATCCGTCTCATAAAGTTGAATGTTTAGCCGTTTCCTTCTGTTCAAATCCTTCAGTTTCAGCCCCGTTTACCCCAGTTCCGTTCTGGATTACCCTCCGAGATCAAAAAAGCTATCTGTCTGAATTGCCAGGGATTTGGGGTTTTGAAGGTTGCTCAATCCGCTGTCCGTGGGAATAAATGGCATCTGCGCTGATTAGCGATCTCTGTGTTCCGAATTTGCACGGATTTTCGCAGGATAAGAAAAAGAGGCAACAGGGGTTGCGGTCAGGGTTTGCGCATTCTGTGAACTTGCATTTGGCTAAAGGTCGAATTGTACTGCAGAGTAGAAGGATAAAATGCAATGCCAGCGTCGTGGAGGCCGACGCCATTGCTGAGGCAGAGAAAGCGGAAGATCCTTTTAAATCCCTGAGTCAAGTGTGTGCAGTGCTGGGAAGTCAGTGGGGTGACGAAGGAAAGGGCAAGCTCGTTGACGTTTTAGCACAGCGCTATGATATTGTGGCCCGTTGTCAGGGAGGGGCAAATGCAGGTCACACCATATACAATAGCGAGGGAAAGAAATTTGCTCTTCACCTTGTCCCTTCTGGAATACTCAATGAAAAGGCCCTATGCGTTATTGGGAATGGAGCCGTTGTGCATCTTCCAGGACTATTTGAAGAGATAGAGGGGCTTGAGTCTAATGGAGTTTCTTGCAAAGGAAGACTGTTGATTTCTGACCGTGCTCATTTACTATTTGATTTCCATCAAATTATTGATGGAATGAGAGAAGCAGAACTTGCAGGGTCGTTAATTGGAACGACAAAAAGAGGGATTGGTCCTTGCTATGCAAGCAAGGCAATCCGGAATGGCATTCGGGTAAGTGATTTGAGGCACATGGATACATTTCCTGAGAAGTTGGATATTCTTTTGCATGATGCGGCATCCCGTTTTAAAGGCTTTGAGTATAGTAAAAGCATGTTACAGACTGAAGTGGAGCATTACAAGAGATATTCTGAGCGAATAGAACCTTATATTACTGACACTGTTCATTTGATGAATGATGCATTCTCAAGCGGGAAGAAGATTTTGGTTGAAGGCGGCCAGGCAACCATGTTAGATGTCGATTTTGGAACTTATCCATTTGTCACTTCTTCTAATCCATCTGCCGGTGGAATCTGTACTGGACTTGGTATTTCTCCAAGACATATTGGTGATATGATTGGTGTGGCTAAAGCATACACAACAAGAGTTGGTTCAGGTCCTTACCCGACAGAACTCTTGGATGAAGCGGGTGAAGATCTTCGATCAGCTGGACATGAATATGGCACCACAACTGGCCGGCCTCGTCGTTGTGGTTGGCTAGATCTGGTTGCACTTAAATATTCTTGTCAAATCAATGGTTTCTCAGCACTTAATCTGACAAAGCTTGACGTACTTTCTGGGTTATCTCGAATTAGGCTTGGGGTAGCCTATAAAGGGCCTGATGGAAATGTGTTGAAATCTTTTCCTGCGGACCTTGACCTTCTGCAAAAAGTAGAGGTTATTTACGAAGATCTGCCTGGATGGCAGGCTGATATATCTAAAGTGAGAAGATATCAAGACTTACCATCTGCTGCTTGCCTCTATGTGGAAAGGATCGAGAGTTTACTTGGAATACCAATCGATTATATTGGTGTTGGGCCAGGCCGTGATGCTTTAATAGTGAAGTAATAGGATCAACAGATTTTAAAGTCATCCCATGGAGTTTGCTGTCCAAGAAGCAGGTCAAAAACATGCATGGGAACTTTTAATGGCCTAACATTCCCTGTGTCATTTTTGGCTTTTGTGACTTTTTTATATGTCCCTTGTCCAGAACACTTTGGTTAGTCTAAGCAGGAATAAATTCAAGAAATTATAGGCTCTTGTTTTCTACAATGCATGAGCGTTTGATGACAGAGAGGTATTAGTAACTTGTCACTGCGAAACTGAAAAAGGGTACGTAACCAATAAATCATACTACCGTATCTGAAGAAAAAGCAGCATCTAAAAAGAGAAATTATACTAATGCATATATAGTTTACTTGCATGATAATGATGTGTTTTCAGCTATAAAACACTGTTTGCAGTGGCTGGTAAAACTCAGCTAGTGCAATTGTGTTGGATAATGAAATTTTTCATCATCTCCTGATTTTCTGCCACTATTTCACCACTGGTTGATAAACTCACAGTGTTTCTAGATGATATTTTCAAGATTCTTGGAATTATTGTCCAGTTAATGCCCA

Ace file of the UniGene sp_v2.0_unigene12087 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Adenylosuccinate synthetase 2, chloroplastic n=1 Tax=Ricinus communis RepID=PURA2_RICCO	-	-	77%
FL-Next	sp=Adenylosuccinate synthetase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	94%
Blast2go	adenylosuccinate synthetase	-	-	87%

Source	ECs	Term	Type	e value	Identity
Blast2go	Adenylosuccinate synthase.	EC:6.3.4.4	-	0.0	87%

Source	KEGGs	Term	Identity
Blast2go	Purine metabolism	00230	87%
Blast2go	Alanine, aspartate and glutamate metabolism	00250	87%
Blast2go	Biosynthesis of alkaloids derived from histidine and purine	01065	87%
Blast2go	Metabolic pathways	01100	87%

Source	GOs	Term	Type	Identity
Blast2go	AMP biosynthetic process	GO:0006167	Biological Process	87%
Blast2go	response to cadmium ion	GO:0046686	Biological Process	87%
Blast2go	adenylosuccinate synthase activity	GO:0004019	Molecular Function	87%
Blast2go	magnesium ion binding	GO:0000287	Molecular Function	87%
Blast2go	GTP binding	GO:0005525	Molecular Function	87%
Blast2go	cytosol	GO:0005829	Cellular Component	87%
Blast2go	chloroplast stroma	GO:0009570	Cellular Component	87%
Blast2go	apoplast	GO:0048046	Cellular Component	87%

Source	InterPros	Term	Type	Identity
Sma3	Adenylosuccinate synthetase	IPR001114	-	-
Sma3	Exostosin-like	IPR004263	-	-
Sma3	Epidermal growth factor-like	IPR006210	-	-
Sma3	EGF-like region, conserved site	IPR013032	-	-
Sma3	EGF, extracellular	IPR013111	-	-
Sma3	Adenylosuccinate synthase, active site	IPR018220	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5A8A1

Fln msg: your sequence is longer than subject: 503 - 309

Fln protein: MASALISDLCVPNLHGFSQDKKKRQQGLRSGFAHSVNLHLAKGRIVLQSRRIKCNASVVEADAIAEAEKAEDPFKSLSQVCAVLGSQWGDEGKGKLVDVLAQRYDIVARCQGGANAGHTIYNSEGKKFALHLVPSGILNEKALCVIGNGAVVHLPGLFEEIEGLESNGVSCKGRLLISDRAHLLFDFHQIIDGMREAELAGSLIGTTKRGIGPCYASKAIRNGIRVSDLRHMDTFPEKLDILLHDAASRFKGFEYSKSMLQTEVEHYKRYSERIEPYITDTVHLMNDAFSSGKKILVEGGQATMLDVDFGTYPFVTSSNPSAGGICTGLGISPRHIGDMIGVAKAYTTRVGSGPYPTELLDEAGEDLRSAGHEYGTTTGRPRRCGWLDLVALKYSCQINGFSALNLTKLDVLSGLSRIRLGVAYKGPDGNVLKSFPADLDLLQKVEVIYEDLPGWQADISKVRRYQDLPSAACLYVERIESLLGIPIDYIGVGPGRDALIVK*

Protein Length: 504

Fln nts: AGAAATATTTAGCATCCGTCTCATAAAGTTGAATGTTTAGCCGTTTCCTTCTGTTCAAATCCTTCAGTTTCAGCCCCGTTTACCCCAGTTCCGTTCTGGATTACCCTCCGAGATCAAAAAAGCTATCTGTCTGAATTGCCAGGGATTTGGGGTTTTGAAGGTTGCTCAATCCGCTGTCCGTGGGAATAA_-_ATGGCATCTGCGCTGATTAGCGATCTCTGTGTTCCGAATTTGCACGGATTTTCGCAGGATAAGAAAAAGAGGCAACAGGGGTTGCGGTCAGGGTTTGCGCATTCTGTGAACTTGCATTTGGCTAAAGGTCGAATTGTACTGCAGAGTAGAAGGATAAAATGCAATGCCAGCGTCGTGGAGGCCGACGCCATTGCTGAGGCAGAGAAAGCGGAAGATCCTTTTAAATCCCTGAGTCAAGTGTGTGCAGTGCTGGGAAGTCAGTGGGGTGACGAAGGAAAGGGCAAGCTCGTTGACGTTTTAGCACAGCGCTATGATATTGTGGCCCGTTGTCAGGGAGGGGCAAATGCAGGTCACACCATATACAATAGCGAGGGAAAGAAATTTGCTCTTCACCTTGTCCCTTCTGGAATACTCAATGAAAAGGCCCTATGCGTTATTGGGAATGGAGCCGTTGTGCATCTTCCAGGACTATTTGAAGAGATAGAGGGGCTTGAGTCTAATGGAGTTTCTTGCAAAGGAAGACTGTTGATTTCTGACCGTGCTCATTTACTATTTGATTTCCATCAAATTATTGATGGAATGAGAGAAGCAGAACTTGCAGGGTCGTTAATTGGAACGACAAAAAGAGGGATTGGTCCTTGCTATGCAAGCAAGGCAATCCGGAATGGCATTCGGGTAAGTGATTTGAGGCACATGGATACATTTCCTGAGAAGTTGGATATTCTTTTGCATGATGCGGCATCCCGTTTTAAAGGCTTTGAGTATAGTAAAAGCATGTTACAGACTGAAGTGGAGCATTACAAGAGATATTCTGAGCGAATAGAACCTTATATTACTGACACTGTTCATTTGATGAATGATGCATTCTCAAGCGGGAAGAAGATTTTGGTTGAAGGCGGCCAGGCAACCATGTTAGATGTCGATTTTGGAACTTATCCATTTGTCACTTCTTCTAATCCATCTGCCGGTGGAATCTGTACTGGACTTGGTATTTCTCCAAGACATATTGGTGATATGATTGGTGTGGCTAAAGCATACACAACAAGAGTTGGTTCAGGTCCTTACCCGACAGAACTCTTGGATGAAGCGGGTGAAGATCTTCGATCAGCTGGACATGAATATGGCACCACAACTGGCCGGCCTCGTCGTTGTGGTTGGCTAGATCTGGTTGCACTTAAATATTCTTGTCAAATCAATGGTTTCTCAGCACTTAATCTGACAAAGCTTGACGTACTTTCTGGGTTATCTCGAATTAGGCTTGGGGTAGCCTATAAAGGGCCTGATGGAAATGTGTTGAAATCTTTTCCTGCGGACCTTGACCTTCTGCAAAAAGTAGAGGTTATTTACGAAGATCTGCCTGGATGGCAGGCTGATATATCTAAAGTGAGAAGATATCAAGACTTACCATCTGCTGCTTGCCTCTATGTGGAAAGGATCGAGAGTTTACTTGGAATACCAATCGATTATATTGGTGTTGGGCCAGGCCGTGATGCTTTAATAGTGAAGTAA___TAGGATCAACAGATTTTAAAGTCATCCCATGGAGTTTGCTGTCCAAGAAGCAGGTCAAAAACATGCATGGGAACTTTTAATGGCCTAACATTCCCTGTGTCATTTTTGGCTTTTGTGACTTTTTTATATGTCCCTTGTCCAGAACACTTTGGTTAGTCTAAGCAGGAATAAATTCAAGAAATTATAGGCTCTTGTTTTCTACAATGCATGAGCGTTTGATGACAGAGAGGTATTAGTAACTTGTCACTGCGAAACTGAAAAAGGGTACGTAACCAATAAATCATACTACCGTATCTGAAGAAAAAGCAGCATCTAAAAAGAGAAATTATACTAATGCATATATAGTTTACTTGCATGATAATGATGTGTTTTCAGCTATAAAACACTGTTTGCAGTGGCTGGTAAAACTCAGCTAGTGCAATTGTGTTGGATAATGAAATTTTTCATCATCTCCTGATTTTCTGCCACTATTTCACCACTGGTTGATAAACTCACAGTGTTTCTAGATGATATTTTCAAGATTCTTGGAATTATTGTCCAGTTAATGCCCA

Fln Alignment:

Contig12087___MREAELAGSLIGTTKRGIGPCYASKAIRNGIRVSDLRHMDTFPEKLDILLHDAASRFKGFEYSKSMLQTEVEHYKRYSERIEPYITDTVHLMNDAFSSGKKILVEGGQATMLDVDFGTYPFVTSSNPSAGGICTGLGISPRHIGDMIGVAKAYTTRVGSGPYPTELLDEAGEDLRSAGHEYGTTTGRPRRCGWLDLVALKYSCQINGFSALNLTKLDVLSGLSRIRLGVAYKGPDGNVLKSFPADLDLLQKVEVIYEDLPGWQADISKVRRYQDLPSAACLYVERIESLLGIPIDYIGVGPGRDALIVK
D5A8A1_______________MREAELAGSLIGTTKRGIGPCYASKVIRNGIRVSDLRHMDTFPEKLDILLHDAASRFKGFEYSKSMLQTEVEHYKRYSERVEPYIAVTVHLMHDAFSSGKRILVEGGQATMLDVDFGTYPFVTSSNPSAGGICTGLGIAPRHIGDLIGVAKAYTTRVGSGPYPTELLDEAGENLRSAGYEYGTTTGRPRRCGWLDLVALKYSCQINGFSTLNLTKLDVLSGLSRIRLGVAYKGPDGNVLPSFPADLDLLQQVEVIYENLPGWQADISSVRRYQDLPSAACLYVERIESLLGIPINYIGVGPSRDALIVK

SNPs (tot: 122)

Position	A %	C %	G %	T %	Probability
153	75		25		0.996473
160	25		75		0.996473
177	50		50		1.0
200		75		25	0.996473
201	75		25		0.996473
209	75		25		0.996473
211		25		75	0.996473
215			25	75	0.996473
216	80		20		0.995493
221		20		80	0.995493
222			20	80	0.995493
236	25	75			0.996473
244	50	50			1.0
247		50		50	1.0
253			25	75	0.996473
280	60		40		1.0
288		60		40	1.0
310			75	25	0.996473
314		75		25	0.996473
332		66		33	0.997452
335		33		66	0.997452
343		66		33	0.997452
347		33		66	1.0
352		33		66	0.997452
550		12		87	0.992554
731		92	7		0.999999
734		92	7		0.999999
736			7	92	0.999999
737	7			92	0.999999
738		7		92	1.0
739	7			89	0.999999
742	92	7			0.999999
751	92	7			0.999999
753	7		92		0.999999
754		7		92	0.999999
755		92	7		0.999999
835	91			2	0.993509
836		89		2	0.999999
839		91	8		1.0
844		8		91	1.0
845	8	2		89	0.999999
846	10			89	1.0
853		8		91	1.0
854			8	91	1.0
875		3	6	89	0.998304
882	89		6		0.998304
883		6	93		0.999453
884	89	6	3		0.998304
886	6	3		86	0.995852
887	93		6		0.999453
888		3	10	86	1.0
890		10		86	1.0
892	89	10			1.0
894		7		92	0.999602
925			91	8	0.999766
929			91	8	0.999807
938	9			90	0.999872
947	90		10		0.999898
1123	90			10	0.90298
1131	90	10			0.90298
1132		10		90	0.90298
1136			10	90	0.90298
1141	90	10			0.90298
1142	90		10		0.90298
1473		11		88	0.999937
1498	68		31		1.0
1529	7		78	14	0.999974
1539		75		25	1.0
1545		10		90	0.90298
1561	56			18	1.0
1562		56	18		1.0
1563	43			56	1.0
1564		81	18		1.0
1566		26	20		1.0
1569			18	81	1.0
1570		18		81	1.0
1571	81	18			1.0
1572	18		81		1.0
1574		81	18		1.0
1578	81	18			1.0
1580	18	75	6		1.0
1581		81		18	1.0
1582		81	18		1.0
1583	7	46	46		1.0
1646		20		80	0.999996
1649		80		20	0.999996
1655		70	20	10	0.999998
1657		20	80		0.999996
1684	20	80			0.999996
1708	84			15	0.999986
1774		20		80	0.999996
1802	90		10		0.90298
1812		20	80		0.999996
1847	77			22	0.999999
1851			77	22	0.999999
1875	75		25		1.0
1882		62		37	1.0
1883	12		87		0.992554
1958	60		40		1.0
1966	40			60	1.0
1969	40		60		1.0
1979		10		90	0.90298
1980		10	90		0.90298
1982		90		10	0.90298
1994		55	11	33	1.0
2003		55		33	1.0
2008		11		88	0.923564
2032			28	71	1.0
2033	33	66			1.0
2035			66	33	1.0
2036	66		33		1.0
2040	33		66		1.0
2042	66	33			1.0
2043	33		50	16	1.0
2046	66		33		1.0
2049	33		66		1.0
2050		83		16	0.994512
2051			33	66	1.0
2053	66	33			1.0
2054			33	66	1.0
2075		75		25	0.996473
2076		75	25		0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain