SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene11577

Length: 1276 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene11577

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Rossmann-fold NAD(P)-binding domain-containing protein [Arabidopsis thaliana] dbj\|BAC41977.1\| unknown protein [Arabidopsis thaliana] gb\|AAP37706.1\| At4g31530 [Arabidopsis thaliana] gb\|AEE85924.1\| Rossmann-fold NAD(P)-binding domain-containing protein [Ara	-	-	71%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	31%
Blast2go	rossmann-fold nad -binding domain-containing protein	-	-	82%

Source	ECs	Term	Type	e value	Identity
Blast2go	3-beta-hydroxy-Delta(5)-steroid dehydrogenase.	EC:1.1.1.145	-	0.0	82%

Source	KEGGs	Term	Identity
Blast2go	Steroid hormone biosynthesis	00140	82%
Blast2go	Biosynthesis of terpenoids and steroids	01062	82%
Blast2go	Biosynthesis of plant hormones	01070	82%
Blast2go	Metabolic pathways	01100	82%

Source	GOs	Term	Type	Identity
Blast2go	steroid biosynthetic process	GO:0006694	Biological Process	82%
Blast2go	oxidation-reduction process	GO:0055114	Biological Process	82%
Blast2go	binding	GO:0005488	Molecular Function	82%
Blast2go	3-beta-hydroxy-delta5-steroid dehydrogenase activity	GO:0003854	Molecular Function	82%
Blast2go	chloroplast envelope	GO:0009941	Cellular Component	82%
Blast2go	chloroplast stroma	GO:0009570	Cellular Component	82%

Source	InterPros	Term	Type	Identity
Sma3	NAD-dependent epimerase/dehydratase	IPR001509	-	-
Sma3	Alcohol dehydrogenase superfamily, zinc-type	IPR002085	-	-
Sma3	NmrA-like	IPR008030	-	-
Sma3	Male sterility, NAD-binding	IPR013120	-	-
Sma3	Alcohol dehydrogenase, C-terminal	IPR013149	-	-
Sma3	Quinone oxidoreductase putative, YhdH/YhfP	IPR014188	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: A9NPJ0

Fln msg: No stop codon before M and low homology subject, your sequence is longer than subject: 288 - 254

Fln protein: MENLRNRIVCVKTDAESPALSREEQESIKVSSKFVLVVGGTGGVGQLVVARLLDRGVRTRALVRDPEKAETLFGKQDSDKLQILTGDTRHPEELNASVFEGVTDVICCTGTTAFPSKRWDGDNTPERTDWDGIRNLVAALPSSVKRFVLVSSVGVTKFNKLPWSIMNLFGVLKFKKMGEDFLQSSGIAFTIIRPGRLTDGPYTSYDLNTLLKATAGTRRAVLIGQGDELVGEASRIDVAEACIQALYLECTIGQTYEINSVEGVGPGTDPKKWEKIFRDANLKELNA*

Protein Length: 289

Fln nts: ATGGGGAATCTATGATTCAATTCGCAATTTTAACGCCACAGAAAGCTCAGAATGGCCGCATGTTTCAACACACTGTTGCCACATCTTCCACACCTCTGGCGGATTGAATCAGAAGGGGAGAACAATTACGGTTGTTAATAGTCATGTTGGGCTAAGGGCTGGCAGATTCTTGATTGGTCAAGGAAATGAGCTTAAGTTCAAGCGGAAGGTGACG_-_ATGGAGAACCTGCGAAATAGAATTGTGTGCGTGAAAACAGATGCTGAATCGCCTGCGCTGTCACGTGAAGAACAAGAATCCATAAAGGTTTCGTCTAAATTTGTGCTGGTTGTCGGAGGCACTGGCGGAGTCGGGCAGCTTGTTGTGGCAAGATTGCTAGACCGAGGGGTCAGAACACGTGCACTGGTTCGTGATCCAGAAAAGGCAGAGACTTTATTTGGTAAACAGGATAGTGATAAGCTGCAGATTTTGACAGGTGATACACGGCATCCTGAGGAACTTAATGCATCAGTTTTTGAGGGAGTCACAGATGTCATTTGCTGTACTGGAACGACAGCTTTTCCATCCAAGCGCTGGGATGGGGATAACACCCCTGAAAGGACAGATTGGGATGGTATCCGCAATCTTGTAGCTGCATTGCCTTCATCAGTGAAGAGATTTGTTCTTGTCTCATCGGTTGGAGTTACAAAGTTCAACAAACTTCCTTGGAGCATTATGAACCTCTTTGGAGTGCTAAAGTTCAAAAAGATGGGGGAGGATTTTCTTCAGAGTTCTGGTATTGCTTTCACAATTATTAGACCAGGAAGACTTACGGATGGGCCATATACTTCTTATGATCTGAACACTTTGCTTAAAGCAACAGCAGGCACACGCCGTGCTGTATTGATTGGACAAGGGGATGAACTGGTGGGAGAGGCTAGCAGGATTGATGTAGCTGAAGCATGCATACAAGCATTATATTTAGAGTGTACGATTGGTCAGACTTATGAGATCAACTCAGTTGAGGGAGTTGGACCAGGAACTGATCCAAAGAAGTGGGAGAAGATTTTTAGAGATGCTAACTTGAAAGAGTTGAATGCATGACTGAAGCATTTGGCGATTGTATTTAAAGATAACTCTGCCATGGGCTTTTCTGAAGGCATTTCTCTGCTTTGATCTACATTATGAATATGACAAAATCATAATCTTACAGTTTATAGCTTTTATTTCTGGGTGTTCAAGATGTTTTGTTGGTTTTTTTCTATATGATGTGAGAAGGAAATGCTTTGTTGATTGACTGAA

Fln Alignment:

Contig11577___RLLDRGVR--TRALVRDPEKAETLFGKQDSDKLQILTGDTRHPEELNASVFEGVTDVICCTGTTAF------PSKR------WDGDNTPERTDWDGIRNLV-AALPSSVKRFVLVSSVGVTKFNKLPWSIMNLFGVLKFKKMGEDFLQSSGIAFTIIRPGRLTDGPYTSYDLNTLLKATAGTRRAVLIGQGDELVG----EASRIDVAEACIQALYLECTIGQTYEINS--VEGVGPGTDPK
A9NPJ0_______________KLKERGGQFVSRGFVRTEESKQKIGGGDD-----VFVGDVRQPETLTP-VFQGVDALVIVTSAVPKMKPGFDPSKGGRPEFYFEDGCYPEQVDWIGQKNQIDAAKAVGAKQIVLVGSMGGTNPNH-PLNSLGNGKILIWKRKAEQYLADSGIPYTIIRAGGLVD--------------KEGGLRQLLVGKDDELLATETKSVPRADVAEVSVQALLIEEAKNKAFDLASKPEEAGSPTTDFK

SNPs (tot: 7)

Position	A %	C %	G %	T %	Probability
937	88	11			0.923564
940	88		11		0.923564
944	88	11			0.923564
946	88			11	0.923564
949	88			11	0.923564
954		11		88	0.923564
1096	54		45		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain