SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene11405

Length: 1760 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene11405 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	SUMO protease [Arabidopsis thaliana]	-	-	61%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	93%
Blast2go	sumo protease	-	-	80%

Source	GOs	Term	Type	Identity
Blast2go	protein desumoylation	GO:0016926	Biological Process	80%
Blast2go	proteolysis	GO:0006508	Biological Process	80%
Blast2go	SUMO-specific protease activity	GO:0016929	Molecular Function	80%

Source	InterPros	Term	Type	e value	Identity
Sma3	Peptidase C48, SUMO/Sentrin/Ubl1	IPR003653	-	0.0	-
Sma3	Intermediate filament protein, conserved site	IPR018039	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5AAV2

Fln msg: Overlapping hits, possible frame ERROR between 501 and 501, and overlapping frame ERROR between 536 and 527, your sequence is longer than subject: 323 - 209

Fln protein: MLEHGREAQPSDDREAKVGNSQPRYKELFEETKKRDFKLKRLELELELATTKLNALQFTKKKLFKAKEKEEAAVEHKSWIEQALEDAFAPLNDDEEADVAHALKGGNSREVLVMHENSNIEITKGLLQCLLPGGWLNDEVINLYLELLKEREKREPDKxxKCHFFNxxxxxxLYNPNTRYDYKAVRRWTTQRKIGYSLMDCDKIFVPIHKEIHWCLAIIDMKAKKFQYLDSLRGSDAHVLDVLVKYITDEAKDKTGQDLDVSSWETQLVEDLPQQENGSDCGMFMIKYADFHSQGLPLSFFQTHMPYFRKRTAKEILRLKAE*

Protein Length: 324

Fln nts: GATGCAGAGAGGGGTACAGAGGCT_-_ATGCTGGAGCATGGCCGAGAAGCACAGCCCAGTGACGATAGAGAAGCGAAGGTGGGGAACAGTCAGCCTCGGTATAAGGAATTATTTGAAGAAACTAAAAAGAGGGATTTCAAATTGAAGCGCTTAGAGTTGGAGCTCGAGTTGGCCACCACGAAGCTTAATGCATTGCAGTTTACAAAGAAGAAGCTGTTCAAGGCTAAGGAAAAAGAAGAAGCAGCTGTGGAACACAAATCATGGATAGAGCAGGCATTGGAGGATGCATTTGCACCACTTAATGATGATGAAGAAGCAGATGTAGCACATGCTTTAAAGGGTGGGAATAGTAGAGAAGTGTTGGTGATGCATGAAAATTCTAATATTGAAATAACAAAAGGATTATTGCAATGCTTGTTGCCCGGTGGATGGTTGAATGACGAAGTCATAAACTTATACCTAGAATTGTTAAAGGAGAGGGAGAAACGAGAGCCAGATAAAxxxxxxAAATGCCACTTCTTCAATACxxxxxxxxxxxxxxxGCTTTACAATCCAAACACGAGATATGATTACAAAGCTGTCAGGAGATGGACCACGCAAAGGAAGATTGGATACAGCCTTATGGATTGTGATAAGATTTTTGTTCCGATTCACAAGGAAATTCACTGGTGTTTGGCTATCATTGATATGAAGGCGAAGAAGTTCCAATATCTTGACTCACTTAGAGGAAGCGATGCACATGTCTTGGATGTTTTGGTTAAATATATTACAGATGAAGCAAAGGATAAAACCGGCCAGGATCTAGATGTAAGCTCTTGGGAGACACAATTAGTTGAGGACCTTCCTCAACAGGAAAATGGGTCAGATTGTGGAATGTTCATGATCAAGTATGCAGATTTCCATAGTCAGGGGTTGCCCCTCAGTTTCTTCCAGACACACATGCCTTACTTCCGCAAGAGGACCGCAAAAGAGATTTTAAGACTGAAGGCGGAATGA___TTTTTTTATGTTCAAATGAGAGGAGATACTTATTGGTTTGTTGATGTAGCTTTTAATCTCTGTTGTTTGGATTCTTTCTGCATTCTAGTTGGTACAGTTTTTCTTAGAAGTTGTCATATTTACTGACATACTTGCAGAATTACAGCTGCCCTTCTAGGAGCTTCAATTATGTATTCTGCAACACTGGGAATTGCACCACTTCTATTCCAGCTGTGTGTAGGTTATTGCGTTTTAGATTTAAAACCTGCAATATGAGTGGGCATTGGGAATGTTGTGAGTTGCAGAGATTCGGGAACCATTTTTAGATTCTGCATAAAGCCAGTAAAGAGGCTGACTTGAGGTTGCTGTTTCATTATCAAGGTTCCATTTCAAGTAAACTTCATAGGAATCTGGTCCATTTTTCATTTGTTTCAGTGGACAGAGGCTATGCGGTTGTTTTCTTCCACAAACGAAATTTCCTCACAGTCAAGCTGAGGGTTTGAACATTTCATTTTTGTGCCTGGAACGATGCTATGTTACAGATGTATACTTGTGTGGAGCATGAGGAAAATTTTCAGCCTAAGGATTGCCGCATTCTGAATGTATGGCAACAGTATAGATAAGAATTATTGTAAAATACAGTTATGTGAAAGTTTATGTACTTAATTGGAAGACGTATATGCTTATACAAGGATGACGGAGACCATCTATATATTAGCAGTGATAAGCAGTAACAATTTGTGATTTCCATAGTTGTAGAAATAAATGCTTTATACATGGATTGATGGAG

Fln Alignment:

Contig11405___MHENSNIEITKGLLQCLLPGGWLNDEVINXXXXXXXXXXXXEPDKFLKCHFFNTFxxxKLYNPNTRYDYKAVRRWTTQRKIGYSLMDCDKIFVPIHKEIHWCLAIIDMKAKKFQYLDSLRGSDAHVLDVLVKYITDEAKDKTGQDLDVSSWETQLVEDLPQQENGSDCGMFMIKYADFHSQGLPLSFFQTHMPYFRKRTAKEILRLKAE
D5AAV2_______________MHENSNIEITREILQCLLPGGWLNDEVINLYLELLKEREKREPDKFLKCHFFNTFxxxKLYNPNTKYEYKAVRRWTTPRKIGYSLIDCDKIFVPIHKEIHWCLVIIDMKEKKFQYLDSLGGDDAHVLDVLARYITDEAKDKTGKDLDVSSWEMELVEDLPQQENGSDCGMFMIKYADFHSRGLPLSFFQTHMPYFRKRTAKEILRLKAE

SNPs (tot: 31)

Position	A %	C %	G %	T %	Probability
783	20	80			0.995493
784		80	20		0.995493
786	20			80	0.995493
793		20		80	0.995493
796	80		20		0.995493
797	80		20		0.995493
800	20			80	0.995493
802			20	20	0.99255
803	20			80	0.995493
804		20		80	0.995493
805			20	80	0.995493
839	25			75	0.996473
840		75	25		0.996473
843	25		75		0.996473
844	75	25			0.996473
851	75		25		0.996473
852		75	25		0.996473
854		25	75		0.996473
858	25		75		0.996473
859		25	75		0.996473
868		33		66	1.0
903		20		80	0.995493
1014	16			83	0.994512
1015	16			83	0.994512
1017	66		33		1.0
1018	83			16	0.994512
1117	25			75	0.996473
1119	25	75			0.996473
1186	33	66			0.997452
1597		16	83		0.994512
1598	16	83			0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain