SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene11174

Length: 1116 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene11174 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Serine/threonine-protein kinase RIO1 n=1 Tax=Nicotiana tabacum RepID=Q75XU7_TOBAC	-	-	80%
FL-Next	sp=Serine/threonine-protein kinase RIO1; Nicotiana tabacum (Common tobacco).	-	-	80%
Blast2go	serine threonine-protein kinase rio1	-	-	87%

Source	ECs	Term	Type	e value	Identity
Blast2go	Transferases, Transferring phosphorous-containing groups, Protein-serine/threonine kinases.	EC:2.7.11.-	-	0.0	87%

Source	GOs	Term	Type	Identity
Blast2go	protein phosphorylation	GO:0006468	Biological Process	87%
Blast2go	ATP binding	GO:0005524	Molecular Function	87%
Blast2go	protein serine/threonine kinase activity	GO:0004674	Molecular Function	87%
Blast2go	endomembrane system	GO:0012505	Cellular Component	87%
Blast2go	mitochondrion	GO:0005739	Cellular Component	87%

Source	InterPros	Term	Type	Identity
Sma3	RIO kinase	IPR000687	-	-
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Tyrosine-protein kinase, active site	IPR008266	-	-
Sma3	Serine/threonine-protein kinase Rio1	IPR017407	-	-
Sma3	RIO-like kinase	IPR018934	-	-
Sma3	RIO kinase, conserved site	IPR018935	-	-

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: tr_plants

Fln subject: Q75XU7

Fln msg: Separated hits, possible frame ERROR between 938 and 943, Distance to subject end: 214 aas, your sequence is shorter than subject: 347 - 562

Fln protein: MSDPETQKSVAVVWNHLDDDDDDIDDEEEEEVEVLDVLEWLDLRDDVEARGNTNKPFSYTASVTATKRPNAHGGLHSASAAAGGPRSLQPRTNQHQKYFNHIHAGPLEEWEGMSDIGMSNSVTTAIRENVRKHSVGRIRNNEKADRATVEQALDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYHATKENGEELAIKVYKTSILVFKDRERYVQGDFRFRHGYCKHNPRKMVKTWAEKEMRNLSRLNAAGIRCPTPMLLRLHILVMEFIGTNGWAAPRLKDAALxxxNKLRECYLEVVIMMRTIYQKCKLVHGDLSEYNILYHEGHLYCIDVSQSVDLDHPRALD

Protein Length: 348

Fln nts: AGAAACGAAAACCGAAGAGACACACAAACGTTTTTGCAAGGTTTTAGAATCGAAAACCGGAGAGATACAAACACGCT_-_ATGAGTGATCCGGAGACACAGAAAAGCGTGGCAGTTGTATGGAATCATCTGGATGATGACGATGACGATATTGACGATGAGGAAGAGGAGGAAGTTGAGGTATTGGATGTATTAGAGTGGTTGGATCTTCGAGATGATGTTGAAGCAAGAGGGAATACGAACAAACCCTTTTCTTACACTGCGTCTGTTACTGCTACAAAACGCCCTAATGCCCATGGAGGTCTTCATTCTGCCTCAGCAGCAGCAGGAGGTCCTCGCTCCCTGCAGCCACGTACCAATCAGCACCAGAAATACTTCAATCACATCCATGCCGGACCCCTAGAGGAATGGGAAGGCATGTCAGATATTGGTATGTCAAACTCTGTCACAACAGCGATTCGTGAAAATGTTCGCAAGCATTCTGTGGGACGCATTCGAAACAATGAGAAAGCAGATCGAGCTACTGTTGAACAGGCACTCGATCCTAGAACACGCATGGTCTTGTTCAAAATGTTAAATCGAGGTGTTTTTCATGACATAAATGGGTGCATTTCTACGGGTAAAGAAGCAAATGTGTACCATGCAACAAAAGAGAATGGAGAGGAGCTGGCTATCAAAGTCTATAAAACTTCTATTCTTGTATTCAAGGACAGGGAAAGGTATGTTCAAGGAGACTTCCGTTTCAGGCATGGGTATTGCAAGCACAATCCAAGAAAAATGGTGAAGACTTGGGCAGAAAAGGAAATGAGAAATCTTTCAAGGCTGAATGCAGCTGGCATCCGGTGTCCAACACCAATGCTTCTACGACTTCATATTCTTGTCATGGAATTCATAGGAACAAATGGTTGGGCTGCACCTCGACTCAAAGATGCTGCCTTGTCxxxxxxGAATAAATTACGTGAATGTTATTTAGAGGTTGTCATCATGATGCGTACCATATATCAGAAATGCAAACTTGTGCATGGAGATCTAAGTGAATACAATATTCTTTATCATGAGGGCCATTTGTATTGTATTGATGTTTCTCAGTCTGTAGACCTTGATCATCCACGTGCGTTAGAC

Fln Alignment:

Contig11174___SLQPRTNQHQKYFNHIHAGPLEEWEGMSDIGMSNSVTTAIRENVRKHSVGRIRNNEKADRATVEQALDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYHATKENGEELAIKVYKTSILVFKDRERYVQGDFRFRHGYCKHNPRKMVKTWAEKEMRNLSRLNAAGIRCPTPMLLRLHILVMEFIGTNGWAAPRLKDAALSxxNKLRECYLEVVIMMRTIYQKCKLVHGDLSEYNILYHEGHLYCIDVSQSVDLDHPRALD
Q75XU7_______________ALQPLSNRTQKFSNHIRASPLEEWEGRIKVGMSNSVTTAIRGSVRDMAIGKTKTTEKADRATVEQAIDPRTRMVLFKMLNRGVFHDINGCISTGKEANVYHATKADGQELAIKVYKTSVLVFKDRDRYVQGDYRFRYGYCKHNPRKMVKTWAEKEMRNLMRLRAAGIRCPAPLLLRLHVLVMEFIGKGGWAAPRLKDAALSxxDKLRECYVEIIMAMRTLYQKCKLVHGDLSEYNILYYEGHLYVIDVSQSVDLDHPHALD

SNPs (tot: 39)

Position	A %	C %	G %	T %	Probability
348	12			87	0.992554
365	85	14			0.993533
366	85	14			0.993533
367		14		85	0.993533
369		87		12	0.992554
401	85			14	0.993533
426	80		20		0.995493
488	16	83			0.994512
702		85		14	0.993533
806	20	80			0.999996
877		33		66	1.0
878		12		87	0.992554
879	12		87		0.992554
930		12		87	0.992554
931	87			12	0.992554
932		87	12		0.992554
935		87	12		0.992554
936		87		12	0.992554
937	87		12		0.992554
939		12		87	0.992554
941		87	12		0.992554
947		87	12		0.992554
948			12	87	0.992554
952			12	87	0.992554
953	12	87			0.992554
955			12	87	0.992554
1004		85		14	0.993533
1005	85		14		0.993533
1007		85	14		0.993533
1010	20	80			0.995493
1015	20		80		0.995493
1016	20			20	0.99255
1017		80	20		0.995493
1018			20	80	0.995493
1020		20		80	0.995493
1021	20			80	0.995493
1023			25	75	0.996473
1024	20		80		0.995493
1025		20		80	0.995493

SSRs (tot: 1)

Start position	End position	Sequence	Length
793	804	CTG CTG CTG CTG	12

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain