SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene10847

Length: 1112 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene10847 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Gag-pol polyprotein n=7 Tax=Glycine max RepID=Q84VH6_SOYBN	-	-	40%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	38%
Blast2go	gag-pol polyprotein	-	-	60%

Source	GOs	Term	Type	e value	Identity
Blast2go	binding	GO:0005488	Molecular Function	0.0	60%

Source	InterPros	Term	Type	Identity
Sma3	Peptidase S8/S53, subtilisin/kexin/sedolisin	IPR000209	-	-
Sma3	BTB/POZ-like	IPR000210	-	-
Sma3	Chromo domain/shadow	IPR000953	-	-
Sma3	Aminoacyl-tRNA synthetase, class I, conserved site	IPR001412	-	-
Sma3	Integrase, catalytic core	IPR001584	-	-
Sma3	Leucine-rich repeat	IPR001611	-	-
Sma3	Zinc finger, CCHC-type	IPR001878	-	-
Sma3	Phosphotransferase system, HPr serine phosphorylation site	IPR002114	-	-
Sma3	Short-chain dehydrogenase/reductase SDR	IPR002198	-	-
Sma3	Glucose/ribitol dehydrogenase	IPR002347	-	-
Sma3	HhH-GPD domain	IPR003265	-	-
Sma3	Retrotransposon gag protein	IPR005162	-	-
Sma3	BTB/POZ fold	IPR011333	-	-
Sma3	BTB/POZ	IPR013069	-	-
Sma3		IPR013084	-	-
Sma3	Reverse transcriptase, RNA-dependent DNA polymerase	IPR013103	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-
Sma3	Carbohydrate kinase, FGGY, conserved site	IPR018483	-	-

Full-Lengther Next Prediction

Fln status: Putative N-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LKX7

Fln msg: Distance to subject end: 25 aas, atg_distance in limit (1-15): atg_distance = 9, W2: There is no M at the beginning, your sequence is shorter than subject: 370 - 407

Fln protein: RRDLGCHSCDQGTEVIFRSNGCCIRDLDTGETVIKGKRTPNNLYIFEEGQQQCYLSKDDEHWLWHQRLGHLSFSQIRKACKYQAVRDLPDIKIPDNTICKSCQFGKQTRTNFPEKEGSASRPLELVHTDTCGPFRKRTPRGEEYLILFIDDFSRFVWLGLMKHKDEAFEKFKSFNALVENESGYKIKCLRSDRGGEFTSNEFFDFCEEHGIRREFSTARTPQQNGVVERMNRTVQQMARAMLDESGTPATFWGEAAHAAVVILNKTNVRVNSTQTPHELWKGETPSVKHFKIFGSKCYIKNTDEQLGKLEPRADEGILLGYSPHSKAYKCYNKRLGRIVDSIDVVVDEKGYIPREVHHEDIEEDEGYPQK

Protein Length: 371

Fln nts: CGTCGTGACCTTGGCTGTCACTCATGTGATCAAGGAACAGAGGTAATATTTAGATCAAATGGATGTTGCATTCGTGATCTTGATACAGGTGAAACAGTGATCAAAGGAAAAAGAACTCCAAATAATCTATATATATTTGAAGAAGGACAACAACAATGCTATCTAAGCAAAGATGATGAACACTGGTTATGGCATCAAAGGTTGGGGCATCTAAGCTTTTCACAAATAAGGAAAGCATGCAAATATCAGGCAGTTCGCGATCTTCCAGATATCAAAATTCCAGACAACACCATATGCAAATCATGTCAGTTTGGAAAGCAAACAAGAACTAATTTTCCAGAGAAGGAAGGATCAGCAAGCAGGCCTCTTGAGCTAGTACATACAGATACATGTGGCCCTTTCAGAAAAAGGACACCTCGGGGAGAAGAATACTTGATATTATTTATTGATGATTTCTCCAGGTTTGTCTGGTTAGGACTTATGAAGCACAAAGATGAAGCATTCGAAAAATTCAAATCCTTCAATGCCTTGGTTGAAAATGAATCAGGATACAAAATTAAGTGCCTCAGATCTGACAGAGGTGGGGAGTTTACCTCGAATGAATTTTTCGATTTCTGTGAAGAACATGGGATAAGAAGAGAATTCTCTACAGCTAGAACTCCTCAGCAAAATGGAGTTGTGGAAAGAATGAACAGAACAGTCCAACAAATGGCTCGTGCTATGCTTGATGAATCAGGTACTCCTGCTACTTTTTGGGGTGAAGCTGCACATGCTGCGGTGGTTATTCTCAACAAAACAAATGTCCGGGTGAACAGCACTCAAACCCCTCATGAGCTCTGGAAAGGTGAGACTCCTTCTGTGAAGCACTTCAAAATCTTTGGGAGCAAATGTTATATTAAAAATACTGATGAGCAACTTGGCAAGCTTGAACCTAGAGCTGATGAAGGCATACTTCTTGGCTATTCTCCACATAGCAAAGCATACAAGTGCTACAACAAAAGACTTGGCAGGATTGTTGACAGTATTGATGTAGTGGTTGATGAGAAGGGATACATTCCTAGGGAAGTCCATCATGAAGACATCGAAGAAGATGAAGGGTACCCTCAAAAGAC

Fln Alignment:

Contig10847___DQGTEVIFRSNGCCIRDLDTGETVIKGKRTPNNLYIF---EEGQQQCYLSKDDEHWLWHQRLGHLSFSQIRKACKYQAVRDLPDIKIPDNTICKSCQFGKQTRTNFPE-KEGSASRPLELVHTDTCGPFRKRTPRGEEYLILFIDDFSRFVWLGLMKHKDEAFEKFKSFNALVENESGYKIKCLRSDRGGEFTSNEFFDFCEEHGIRREFSTARTPQQNGVVERMNRTVQQMARAMLDESGTPATFWGEAAHAAVVILNKTNVRVNSTQTPHELWKGETPSVKHFKIFGSKCYIKNTDEQLGKLEPRADEGILLGYSPHSKAYKCYNKRLGRIVDSIDVVVDEK
B8LKX7_______________NQGYKVEFYKDKCLIKDINSNYKVVASGYVENGLYKFGRFTSNEKALVAEVDNVSRLWHERMGHLNYKSLSLMKRFEMVYGLPKIS-QSQGVCEGCMSGKQHMEKFIKGKSWRAKTPLHLVHSDLMGPLEHPSISGSRYVLTFIDDYSRRIWVYFLKNKDEVFEKFKEFKAFVEKQSGKSIKILRTDNGKEYVNKEFDHYCKYNGIKREHTVPYTPQQNGVAERKNRTLMEMARCMLHARNMDPKFWAEAINTATYIVNRTPTIAVKHKTPEEAWSRRKPTVSHFKVFGCDVYVHIPDEKRKKLDRKSHKCIMVGYSETSKAYRVYDPEKNEILIRRDVIFDER

SNPs (tot: 34)

Position	A %	C %	G %	T %	Probability
806		88		11	0.923564
807	11			88	0.923564
808		11	88		0.923564
809	88			11	0.923564
813		11	88		0.923564
814	88	11			0.923564
815		11		88	0.923564
816	11			88	0.923564
857			87	12	0.992554
866		87		12	0.992554
875			71	28	1.0
880		85	14		0.993533
884		85		14	0.993533
899	14		85		0.993533
908		71		28	1.0
913	83		16		0.994512
914		80		20	0.995493
917	20			80	0.995493
918		50		50	1.0
928	75		25		0.996473
932	50		50		1.0
938	75			25	0.996473
944		25		75	0.996473
946		25		75	0.996473
950	25	25		50	0.995533
952	25		75		0.996473
970		66		33	0.997452
973	33	66			0.997452
982		33		66	0.997452
983	66		33		0.997452
1001		33		66	0.997452
1003		33		66	0.997452
1004		33		66	0.997452
1005	33	33		33	0.997753

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain