SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene10789

Length: 2267 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene10789
CGTCGTGACCTTGGCTGTCACTCAGATGGTTGAGGGAATGGTTTGGCTCGATTTCTATGGAGTGTTTGAGTAATTATCAGTGGTGCTAATAAATGGGTTTTTAAGTCCATTATAATGGGTTATCTGCGTCGTGACCTTGGCTGTCACTCAATTTATTCTCTGTATTCATCTGATCATGATGGATGTTTTAATGAGGGTTTTTTATTACTGATTATCACTGTATTATTTCATAATTCTCCTGCATTTTCTGAGTTGGGTAGACTACTAAAATTTATCAGAAGCATCATATGAAGGGCGAGGAGGGGGTCAATTGTCATTAGGTAGGTCTGCTCACCAAACATACACAAAAGGAGGAGGAGAATCTGTCTGTTGGTTAGCCACATTGCCTGTGTTTATAGTCTTTTTGGTTCCATTACAATGCAGTACTGTTGGATTCTCTGCCATTTTTTCTTTCTGATTATTTAAACTGCTTGTCAATAACGTATTGCATGTTTGAGACTTCTGGGTCCGCCTGTTTCCAATTTTTTTGTGGGTTCATTGTATCTTTTTGAGAGGGATCTAAAGACATTGTTGCGCGTTCCTAGATAGGATCAAATTAGAGCTGTCTCTGTCTCAACTCTGGATCTATTAGGTTTCTGATCTGGCTCTGCTGGGGTATTGTTGTCTGCGAGCGCTTTCTCTGTGGGCTCTCAGATGTGGGCATGTACCATTCTGTCATAGACCAGATTCATGCGTTTTTTATGTTATTTCCCTATGTGGAAACAGTTTCAGGCAGAAAGTCCCAAGGATGCTCATAGTTGTGCTTCAATGGATAGTCCAATGTCACTGCAGGGACCTTCTGACGAATTTCAGTTGGATAAAAGATGGCTCATTGATCCCAAACTACTGTATGTAGGTCCAAGGATCGGGGAAGGTGCTCATGGAAAAGTCTATGAGGGCAAGTACCGGGACCAAAATGTTGCTATTAAAATAATTCAGGCTGGAGATGTTCCAGAAGAGATCGCCAGACGTGAGGCACGCTTTGCAAGAGAGGTTGCATTGCTAGCACGTGTGCAACACAAAAATCTAGTGAAGTTTATTGGCGCCTGCAAGGAACCTGTTATGGTCGCGGTTACAGAGCTTTTAGTAGGTGGGTCGCTCCGGAAATATATGTTGAGTATACGGCCAAAACGCTTAGAGTTGCGGCTGGCAGTAAGTTTTGCATTGGATATTGCCCAAGCCATGGAATGCTTACATGCACACGGCATCATTCACCGCGATCTTAAGCCTGATAATTTATTACTGACAGCAGATCAGAAAAAAGTTAAACTGGTAGATTTTGGGCTGGCAAGGGAAGAGACACTAACGGAAATGATGACTGCAGAGACTGGCACTTATAGATGGATGGCTCCTGAGTTGTACAGTACTGTTACATTAAGACACGGAGAGAAAAAACATTATAATCACAAGGTAGATGCATACAGTTTTGCTATAGTCTTATGGGAGCTGTTGACAAATCGAATGCCGTTTGAAGGAATGTCAAATCTGCAAGCAGCATATGCTGCTGCTTTTAAGAATGTGAGACCAAGTTCAGAGAATCTTCCAGAGGAAATGGCTTCAATCTTGGAATCCTGCTGGGCACAGGATCCCAACAGCCGGCCCAACTTCAGTCAAATAGTGAGAAAACTTAGTTCTTTTCTTTCATCCCTTTCCGATCCAGAGAACATGCCTCCACCAGTGCTTATTGCTTCAAAGAATAGCATTCTGCCACCAGAGTCTCCAGGGACAAGTTCATTGATGGCAAAGCGGGGTGAACAAGGTGAAGATGCCAATGGCAAGTCAGAAAACAAATCACGGGGATTCCTTCCTTGTTTTAGTCACTGCTTCCAATCAGATGCTGAAAATGGATTGATAGAATGATGCAAGGATCCATGCCCCTTTCCAATGAGATGCATTAACAATTTCAGGGCTTATCAATGCAGTCACTTTCAGATAGTGCATTTGCTGTCTCTTATTTTATATCTCTTGTTCAAGTGCAAACTATTCCCTCCAATTCGACAAGAACTAACTTGTTTTGTATTCTTTTCTTCTGTCGTTAACTTTCTGTTAGCAATCTATTGTTGCACAAAATTTATATTTTTATATAGTGACTTCCAACTAAAATGCAGGTATATGTTAAACTCTTTAATTAGGAACTGCAATTGATTATGGTTATTGGGGTTTTCGTATGCGAGATTTGGTCAGACACTGGGAGTGCAGTTAGGAATAGGAATGAATGCTAACTGGAT

Ace file of the UniGene sp_v2.0_unigene10789 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	flag-tagged protein kinase domain of putative mitogen-activated protein kinase kinase kinase [synthetic construct]	-	-	64%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	95%
Blast2go	protein kinase	-	-	86%

Source	ECs	Term	Type	e value	Identity
Blast2go		EC:2.7.112.1	-	0.0	86%
Blast2go	Mitogen-activated protein kinase kinase kinase.	EC:2.7.11.25	-	0.0	86%

Source	GOs	Term	Type	Identity
Blast2go	protein phosphorylation	GO:0006468	Biological Process	86%
Blast2go	protein serine/threonine/tyrosine kinase activity	GO:0004712	Molecular Function	86%
Blast2go	ATP binding	GO:0005524	Molecular Function	86%
Blast2go	MAP kinase kinase kinase activity	GO:0004709	Molecular Function	86%

Source	InterPros	Term	Type	Identity
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	Serine-threonine/tyrosine-protein kinase catalytic domain	IPR001245	-	-
Sma3	Serine/threonine-protein kinase, ATN1-like	IPR015784	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LR22

Fln msg:

Fln protein: MRFLCYFPMWKQFQAESPKDAHSCASMDSPMSLQGPSDEFQLDKRWLIDPKLLYVGPRIGEGAHGKVYEGKYRDQNVAIKIIQAGDVPEEIARREARFAREVALLARVQHKNLVKFIGACKEPVMVAVTELLVGGSLRKYMLSIRPKRLELRLAVSFALDIAQAMECLHAHGIIHRDLKPDNLLLTADQKKVKLVDFGLAREETLTEMMTAETGTYRWMAPELYSTVTLRHGEKKHYNHKVDAYSFAIVLWELLTNRMPFEGMSNLQAAYAAAFKNVRPSSENLPEEMASILESCWAQDPNSRPNFSQIVRKLSSFLSSLSDPENMPPPVLIASKNSILPPESPGTSSLMAKRGEQGEDANGKSENKSRGFLPCFSHCFQSDAENGLIE*

Protein Length: 391

Fln nts: CGTCGTGACCTTGGCTGTCACTCAGATGGTTGAGGGAATGGTTTGGCTCGATTTCTATGGAGTGTTTGAGTAATTATCAGTGGTGCTAATAAATGGGTTTTTAAGTCCATTATAATGGGTTATCTGCGTCGTGACCTTGGCTGTCACTCAATTTATTCTCTGTATTCATCTGATCATGATGGATGTTTTAATGAGGGTTTTTTATTACTGATTATCACTGTATTATTTCATAATTCTCCTGCATTTTCTGAGTTGGGTAGACTACTAAAATTTATCAGAAGCATCATATGAAGGGCGAGGAGGGGGTCAATTGTCATTAGGTAGGTCTGCTCACCAAACATACACAAAAGGAGGAGGAGAATCTGTCTGTTGGTTAGCCACATTGCCTGTGTTTATAGTCTTTTTGGTTCCATTACAATGCAGTACTGTTGGATTCTCTGCCATTTTTTCTTTCTGATTATTTAAACTGCTTGTCAATAACGTATTGCATGTTTGAGACTTCTGGGTCCGCCTGTTTCCAATTTTTTTGTGGGTTCATTGTATCTTTTTGAGAGGGATCTAAAGACATTGTTGCGCGTTCCTAGATAGGATCAAATTAGAGCTGTCTCTGTCTCAACTCTGGATCTATTAGGTTTCTGATCTGGCTCTGCTGGGGTATTGTTGTCTGCGAGCGCTTTCTCTGTGGGCTCTCAGATGTGGGCATGTACCATTCTGTCATAGACCAGATTC_-_ATGCGTTTTTTATGTTATTTCCCTATGTGGAAACAGTTTCAGGCAGAAAGTCCCAAGGATGCTCATAGTTGTGCTTCAATGGATAGTCCAATGTCACTGCAGGGACCTTCTGACGAATTTCAGTTGGATAAAAGATGGCTCATTGATCCCAAACTACTGTATGTAGGTCCAAGGATCGGGGAAGGTGCTCATGGAAAAGTCTATGAGGGCAAGTACCGGGACCAAAATGTTGCTATTAAAATAATTCAGGCTGGAGATGTTCCAGAAGAGATCGCCAGACGTGAGGCACGCTTTGCAAGAGAGGTTGCATTGCTAGCACGTGTGCAACACAAAAATCTAGTGAAGTTTATTGGCGCCTGCAAGGAACCTGTTATGGTCGCGGTTACAGAGCTTTTAGTAGGTGGGTCGCTCCGGAAATATATGTTGAGTATACGGCCAAAACGCTTAGAGTTGCGGCTGGCAGTAAGTTTTGCATTGGATATTGCCCAAGCCATGGAATGCTTACATGCACACGGCATCATTCACCGCGATCTTAAGCCTGATAATTTATTACTGACAGCAGATCAGAAAAAAGTTAAACTGGTAGATTTTGGGCTGGCAAGGGAAGAGACACTAACGGAAATGATGACTGCAGAGACTGGCACTTATAGATGGATGGCTCCTGAGTTGTACAGTACTGTTACATTAAGACACGGAGAGAAAAAACATTATAATCACAAGGTAGATGCATACAGTTTTGCTATAGTCTTATGGGAGCTGTTGACAAATCGAATGCCGTTTGAAGGAATGTCAAATCTGCAAGCAGCATATGCTGCTGCTTTTAAGAATGTGAGACCAAGTTCAGAGAATCTTCCAGAGGAAATGGCTTCAATCTTGGAATCCTGCTGGGCACAGGATCCCAACAGCCGGCCCAACTTCAGTCAAATAGTGAGAAAACTTAGTTCTTTTCTTTCATCCCTTTCCGATCCAGAGAACATGCCTCCACCAGTGCTTATTGCTTCAAAGAATAGCATTCTGCCACCAGAGTCTCCAGGGACAAGTTCATTGATGGCAAAGCGGGGTGAACAAGGTGAAGATGCCAATGGCAAGTCAGAAAACAAATCACGGGGATTCCTTCCTTGTTTTAGTCACTGCTTCCAATCAGATGCTGAAAATGGATTGATAGAATGA___TGCAAGGATCCATGCCCCTTTCCAATGAGATGCATTAACAATTTCAGGGCTTATCAATGCAGTCACTTTCAGATAGTGCATTTGCTGTCTCTTATTTTATATCTCTTGTTCAAGTGCAAACTATTCCCTCCAATTCGACAAGAACTAACTTGTTTTGTATTCTTTTCTTCTGTCGTTAACTTTCTGTTAGCAATCTATTGTTGCACAAAATTTATATTTTTATATAGTGACTTCCAACTAAAATGCAGGTATATGTTAAACTCTTTAATTAGGAACTGCAATTGATTATGGTTATTGGGGTTTTCGTATGCGAGATTTGGTCAGACACTGGGAGTGCAGTTAGGAATAGGAATGAATGCTAACTGGAT

Fln Alignment:

Contig10789___MDSPMSLQGPSDEFQLDKRWLIDPKLLYVGPRIGEGAHGKVYEGKYRDQNVAIKIIQAGDVPEEIXXXXXXXXXXXXXXXXVQHKNLVKFIGACKEPVMVAVTELLVGGSLRKYMLSIRPKRLELRLAVSFALDIAQAMECLHAHGIIHRDLKPDNLLLTADQKKVKLVDFGXXXXXXXXXXXXXXTGTYRWMAPELYSTVTLRHGEKKHYNHKVDAYSFAIVLWELLTNRMPFEGMSNLQAAYAAAFKNVRPSSENLPEEMASILESCWAQDPNSRPNFSQIVRKLSSFLSSLSDPENMPPPVLIASKNSILPPESPGTSSLMAKRGEQGEDANGKSENKSRGFLPCFSHCFQSDAENGLIE
B8LR22_______________MDSPMSMQGPSDEFQLDKRWLIDPKLLYVGPMIGEGAHGKVYEGKYRDQNVAIKIIQAGDVPEEIARREARFAREIALLARVQHKNLVKFIGACKEPVLVVVTELLLGGSLRKYMLSVRPKRLDLRLAVSFALDIAQAMECLHAHGIIHRDLKPDNLLLTADQKKVKLVDFGLAREETLTEMMTAETGTYRWMAPELYSTVTLRHGEKKHYNHKVDAYSFAIVLWELLTNRMPFEGMSNLQAAYAAAFKNVRPSSENLPEELASILESCWVQDPNSRPNFSQIVRKLNAFLSSLSDPENMPPPMVIASKNSILPPESPGTSSLMAKRGEQSEDANGKSGNKSRGFLPCFSHCFQSDAENGLIE

SNPs (tot: 66)

Position	A %	C %	G %	T %	Probability
342		16	83		1.0
419	90		9		0.999842
426		9	90		0.999842
431	13	86			1.0
433		86	13		1.0
434	9		86		0.999642
435		9		90	0.999842
438			10	90	0.999898
440		5		85	0.999793
522		86	13		0.999973
523	86			13	0.999973
526	86	13			0.999973
527	13			86	0.999973
530		13		86	0.999973
532	13			86	0.999973
533			13	86	0.999973
534		86	13		0.999973
536	13			86	0.999973
537		13		86	0.999973
540	86	13			0.999973
603		15		84	0.999986
604	15			84	0.999986
605	20			80	0.999996
606			20	80	0.999996
607		80	20		0.999996
612			20	80	0.999996
1827			80	20	0.995493
1832	80			20	0.995493
1836			20	80	0.995493
1841		80	20		0.995493
1847			20	80	0.995493
1848	20		80		0.995493
1850	80		20		0.995493
1917		14		85	0.993533
1956		88	11		0.923564
1957	88			11	0.923564
1959		11		88	0.923564
1960	11			88	0.923564
1962	87	12			0.992554
1964	12	87			0.992554
1965	12	87			0.992554
1966		85	14		0.993533
1967		85	14		0.993533
1970	14	85			0.993533
1974	14	85			0.993533
1976		85	14		0.993533
2070	83			16	0.994512
2072		83	16		0.994512
2073		83		16	0.994512
2074		83	16		0.994512
2075	16			83	0.994512
2078			16	83	0.994512
2079		16		83	0.994512
2080	83	16			0.994512
2083	83		16		0.994512
2084		83	16		0.994512
2090		16		83	0.994512
2091		83	16		0.994512
2093		16		83	0.994512
2121			25	75	0.996473
2122		25	75		0.996473
2130	25		75		0.996473
2134	75			25	0.996473
2136	25		75		0.996473
2143	75	25			0.996473
2145		25	75		0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain