SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene10600

Length: 2484 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene10600
ATGGGGATGTTTAATAATGGAGAAGGCAAAGAGAAGGTAGTACAGCATTAACCAGGATGACAACATAGTGAGCTTATTATTTATGGCGTAAAATAAACGAATGAAAACGGCAACCAGAGCACGGATGCAAACAAGAACCAATCTCAGATGCCTCTAGCATCCGCCATGGCAGGCGATGGAAGCATTTTGGCAATGGAACTCCTCGCAATTGGAAGGAGCAGCAGCGGTAACAGTTTCCGTCTGCTCAATTTGAAACCCGGCCGAAAAAAATGTACAGTTCCCCAACCCGTCCGCCTCCGTTCCTACACTCCCAAGAGAGAGCTGAATTTGATGCTGAAATCCTCTTCATACGAACGAACCGGCCGTCTATGTGCTTATCGGAAATTGAACATTCTCTGCGCCAACAACCACGGCAAGGGCGAGGCAGACCACTCTCATCATCATCACCATCACCAACACCATCATCATCACCATCATGATCTCCAGGAGAAGGGGCCATCTTCTGGACACTTGGATGAATGCCAAGAGCGGCAGTTAAACAGGGTTCAGAAGGCCCTCGTATTGCTGGCCAAGGCCACGGGATGGGCCAATTTAGCAGATGCTTTGCGGGAGAATTTCCGTCTCTGCTGTTCTTCTATGGTTTTGTTACTTCTGGCTGCCGTTTGCCCTTACGTCATGCCCAAGACCGCTTCACGATCGGCTCAGAATGCCCTAATTGCACTCGCATTTCCTCTCGTTGGGATATCAGCAGCACTTGATGCATCTATTGACATTGCCGGTGGCAGAGTTAATATCCACGTTCTCATGACTTTAGCTGCATTTGCATCTGTGTTTATGGGAAACAGCCTTGAAGGTGGTTTGCTTCTTGCCATGTTTAACCTTGCTCATATTGCGGAGGAATATTACACAAAGCGAGCAATGGGTGATGTCAAGGCACTAAAGGAAAGCAATCCAGAGTTCGCTCTTGTTTTGGATGCATTTGATTCAGCAGTACCTCATCAGTTTTCCTCAATGGCTTCCAAACGTGTTCCTGTGCATGATTTGGAAGTTGGTTCATACATATTGGTCCGGGCTGGTGAGGCTGTACCAGTTGATGGAGAAGTATGCCAAGGAGTATCTATGGTGACTGTTGAACACTTAACAGGAGAGGCTAACCCACTTGAGAAGCAAGTTGGAGATAGTGTTCCTGGTGGTGCTAGAAACTTGGATGGGATGTTGATAATCAAGGCGACCAAGACGTGGAAGGATTCAACCTTGTCCAAGATTATGCATTTGACTGAAGAAGCACAACTGAACAAACCAAAATTACAGAGATGGCTTGATGAGTTTGGAGAACATTACAGCGAGGCTATTGTGGTGGTATCAGCTGTAGTGGCTCTTTTTGGGCCATTTTTATTCAAGTGGCCATTTATAGGTACTCCAGGAGCCCGAGGATCCATTTACAGGGCATTGGGCCTCATGGTAGCGGCTTCCCCATGTGCATTAGCAGTGGCTCCTCTAGCTTATGCAACTGCAATTAGTGCTTGTGCAAGTAAGGGTATCCTGTTAAAAGGTGGACATGCATTGGATGCACTTGCATCTTGCGATACTATTGCATTTGACAAAACTGGAACTTTAACCACGGGGGGTCTTGTCTGCAAGGGAATTGAACCAATCCATGGACATAGTTTACTTGGGCGGAATGACAAGAATGTTAATGCTTGCTGCAATCCAAGCTGTGAAAACGAAGCTCTTGCGGTGGCATCTGCTATGGAAAAAGGAACCACACATCCCATTGCAAGAGCAGTCGCAGATCACAGCGAGGGAAAAGAACTTCCTCCTGTTTCAATTGAACATTTTGAATCTCTACCAGGCAAAGGTCTAGTTGCTACATTGACTGGCATACAGTCAGGGGATGGTGTTCCTCTAAAGGCCACACTTGGTTCGGTGGAGTATATTTCATCTTTATTTAGAAATGAGTTTGAATCAAGGAAAATCAAAGAGGCAGCAAGTGCTTCTCCTTATGGTCAAGATCTGGTGCATGCAGTACTGTCTGTAAACAATAAGGTCACTCTTTTTCACTTTGAGGATAAAATTCGACCTGGAGCTACAGATGTCGTGTCCACATTAAAAAATAAAGCTGGCCTTCGTTTGATGATGTTGACGGGTGATCATGCATCAAGTGCATCACGGGTTGCTAAGGCTGTTGGTATTGATGAGGTCTATTCTGGTCTTAAGCCTGAGGATAAGCTCCATCAAGTAAAATCTCTGTCACGCAATGGGGCTGGGGGTTTAATTATGGTGGGTGATGGAATAAATGATGCACCAGCTTTGGCTGCTGCAACTGTGGGGATTGTTCTTGCACAACGTGCAAGTGCAACGGCGGTGGCTGTAGCAGATGTTCTTCTCTTAGAGGATAATATCAACAGTGTACCTTTCATTATTGCAAAGGCACGTCAAACAACTTCCTTGGTAAAGCAGAGCGTAGCTGCTTGCATTATCATG

Ace file of the UniGene sp_v2.0_unigene10600 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	putative cadmium/zinc-transporting ATPase HMA1 [Arabidopsis thaliana] sp\|Q9M3H5.2\|AHM1_ARATH RecName: Full=Probable cadmium/zinc-transporting ATPase HMA1, chloroplastic; Flags: Precursor emb\|CAB16773.1\| Cu2+-transporting ATPase-like protein [Arabidopsis t	-	-	60%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	27%
Blast2go	cadmium resistance protein	-	-	80%

Source	ECs	Term	Type	Identity
Blast2go	Copper-exporting ATPase.	EC:3.6.3.4	-	80%
Blast2go	Cadmium-transporting ATPase.	EC:3.6.3.46	-	80%
Blast2go	Calcium-transporting ATPase.	EC:3.6.3.8	-	80%
Blast2go	Zinc-exporting ATPase.	EC:3.6.3.5	-	80%

Source	GOs	Term	Type	Identity
Blast2go	zinc ion homeostasis	GO:0055069	Biological Process	80%
Blast2go	response to light intensity	GO:0009642	Biological Process	80%
Blast2go	calcium ion transport	GO:0006816	Biological Process	80%
Blast2go	cellular copper ion homeostasis	GO:0006878	Biological Process	80%
Blast2go	ATP biosynthetic process	GO:0006754	Biological Process	80%
Blast2go	response to toxin	GO:0009636	Biological Process	80%
Blast2go	ATP catabolic process	GO:0006200	Biological Process	80%
Blast2go	zinc transporting ATPase activity	GO:0015633	Molecular Function	80%
Blast2go	copper-exporting ATPase activity	GO:0004008	Molecular Function	80%
Blast2go	cadmium-transporting ATPase activity	GO:0015434	Molecular Function	80%
Blast2go	calcium-transporting ATPase activity	GO:0005388	Molecular Function	80%
Blast2go	zinc-exporting ATPase activity	GO:0016463	Molecular Function	80%
Blast2go	ATP binding	GO:0005524	Molecular Function	80%
Blast2go	chloroplast envelope	GO:0009941	Cellular Component	80%
Blast2go	integral to membrane	GO:0016021	Cellular Component	80%

Source	InterPros	Term	Type	Identity
Sma3	ATPase, P-type, H+ transporting proton pump	IPR000695	-	-
Sma3	ATPase, P-type, K/Mg/Cd/Cu/Zn/Na/Ca/Na/H-transporter	IPR001757	-	-
Sma3	Haloacid dehalogenase-like hydrolase	IPR005834	-	-
Sma3	ATPase, P-type, heavy metal-(Cd/Co/Hg/Pb/Zn)-translocating	IPR006404	-	-
Sma3	ATPase, P-type, heavy metal translocating	IPR006416	-	-
Sma3	ATPase, P-type, ATPase-associated domain	IPR008250	-	-
Sma3	ATPase, P-type phosphorylation site	IPR018303	-	-

Full-Lengther Next Prediction

Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: B8LQ20

Fln msg: Unexpected stop codon in the beginning of your sequence, Distance to subject end: 59 aas, your sequence is shorter than subject: 828 - 998

Fln protein: MGMFNNGEGKEKVVQH*PG*QHSELIIYGVK*TNENGNQSTDANKNQSQMPLASAMAGDGSILAMELLAIGRSSSGNSFRLLNLKPGRKKCTVPQPVRLRSYTPKRELNLMLKSSSYERTGRLCAYRKLNILCANNHGKGEADHSHHHHHHQHHHHHHHDLQEKGPSSGHLDECQERQLNRVQKALVLLAKATGWANLADALRENFRLCCSSMVLLLLAAVCPYVMPKTASRSAQNALIALAFPLVGISAALDASIDIAGGRVNIHVLMTLAAFASVFMGNSLEGGLLLAMFNLAHIAEEYYTKRAMGDVKALKESNPEFALVLDAFDSAVPHQFSSMASKRVPVHDLEVGSYILVRAGEAVPVDGEVCQGVSMVTVEHLTGEANPLEKQVGDSVPGGARNLDGMLIIKATKTWKDSTLSKIMHLTEEAQLNKPKLQRWLDEFGEHYSEAIVVVSAVVALFGPFLFKWPFIGTPGARGSIYRALGLMVAASPCALAVAPLAYATAISACASKGILLKGGHALDALASCDTIAFDKTGTLTTGGLVCKGIEPIHGHSLLGRNDKNVNACCNPSCENEALAVASAMEKGTTHPIARAVADHSEGKELPPVSIEHFESLPGKGLVATLTGIQSGDGVPLKATLGSVEYISSLFRNEFESRKIKEAASASPYGQDLVHAVLSVNNKVTLFHFEDKIRPGATDVVSTLKNKAGLRLMMLTGDHASSASRVAKAVGIDEVYSGLKPEDKLHQVKSLSRNGAGGLIMVGDGINDAPALAAATVGIVLAQRASATAVAVADVLLLEDNINSVPFIIAKARQTTSLVKQSVAACIIM

Protein Length: 829

Fln nts: ATGGGGATGTTTAATAATGGAGAAGGCAAAGAGAAGGTAGTACAGCATTAACCAGGATGACAACATAGTGAGCTTATTATTTATGGCGTAAAATAAACGAATGAAAACGGCAACCAGAGCACGGATGCAAACAAGAACCAATCTCAGATGCCTCTAGCATCCGCCATGGCAGGCGATGGAAGCATTTTGGCAATGGAACTCCTCGCAATTGGAAGGAGCAGCAGCGGTAACAGTTTCCGTCTGCTCAATTTGAAACCCGGCCGAAAAAAATGTACAGTTCCCCAACCCGTCCGCCTCCGTTCCTACACTCCCAAGAGAGAGCTGAATTTGATGCTGAAATCCTCTTCATACGAACGAACCGGCCGTCTATGTGCTTATCGGAAATTGAACATTCTCTGCGCCAACAACCACGGCAAGGGCGAGGCAGACCACTCTCATCATCATCACCATCACCAACACCATCATCATCACCATCATGATCTCCAGGAGAAGGGGCCATCTTCTGGACACTTGGATGAATGCCAAGAGCGGCAGTTAAACAGGGTTCAGAAGGCCCTCGTATTGCTGGCCAAGGCCACGGGATGGGCCAATTTAGCAGATGCTTTGCGGGAGAATTTCCGTCTCTGCTGTTCTTCTATGGTTTTGTTACTTCTGGCTGCCGTTTGCCCTTACGTCATGCCCAAGACCGCTTCACGATCGGCTCAGAATGCCCTAATTGCACTCGCATTTCCTCTCGTTGGGATATCAGCAGCACTTGATGCATCTATTGACATTGCCGGTGGCAGAGTTAATATCCACGTTCTCATGACTTTAGCTGCATTTGCATCTGTGTTTATGGGAAACAGCCTTGAAGGTGGTTTGCTTCTTGCCATGTTTAACCTTGCTCATATTGCGGAGGAATATTACACAAAGCGAGCAATGGGTGATGTCAAGGCACTAAAGGAAAGCAATCCAGAGTTCGCTCTTGTTTTGGATGCATTTGATTCAGCAGTACCTCATCAGTTTTCCTCAATGGCTTCCAAACGTGTTCCTGTGCATGATTTGGAAGTTGGTTCATACATATTGGTCCGGGCTGGTGAGGCTGTACCAGTTGATGGAGAAGTATGCCAAGGAGTATCTATGGTGACTGTTGAACACTTAACAGGAGAGGCTAACCCACTTGAGAAGCAAGTTGGAGATAGTGTTCCTGGTGGTGCTAGAAACTTGGATGGGATGTTGATAATCAAGGCGACCAAGACGTGGAAGGATTCAACCTTGTCCAAGATTATGCATTTGACTGAAGAAGCACAACTGAACAAACCAAAATTACAGAGATGGCTTGATGAGTTTGGAGAACATTACAGCGAGGCTATTGTGGTGGTATCAGCTGTAGTGGCTCTTTTTGGGCCATTTTTATTCAAGTGGCCATTTATAGGTACTCCAGGAGCCCGAGGATCCATTTACAGGGCATTGGGCCTCATGGTAGCGGCTTCCCCATGTGCATTAGCAGTGGCTCCTCTAGCTTATGCAACTGCAATTAGTGCTTGTGCAAGTAAGGGTATCCTGTTAAAAGGTGGACATGCATTGGATGCACTTGCATCTTGCGATACTATTGCATTTGACAAAACTGGAACTTTAACCACGGGGGGTCTTGTCTGCAAGGGAATTGAACCAATCCATGGACATAGTTTACTTGGGCGGAATGACAAGAATGTTAATGCTTGCTGCAATCCAAGCTGTGAAAACGAAGCTCTTGCGGTGGCATCTGCTATGGAAAAAGGAACCACACATCCCATTGCAAGAGCAGTCGCAGATCACAGCGAGGGAAAAGAACTTCCTCCTGTTTCAATTGAACATTTTGAATCTCTACCAGGCAAAGGTCTAGTTGCTACATTGACTGGCATACAGTCAGGGGATGGTGTTCCTCTAAAGGCCACACTTGGTTCGGTGGAGTATATTTCATCTTTATTTAGAAATGAGTTTGAATCAAGGAAAATCAAAGAGGCAGCAAGTGCTTCTCCTTATGGTCAAGATCTGGTGCATGCAGTACTGTCTGTAAACAATAAGGTCACTCTTTTTCACTTTGAGGATAAAATTCGACCTGGAGCTACAGATGTCGTGTCCACATTAAAAAATAAAGCTGGCCTTCGTTTGATGATGTTGACGGGTGATCATGCATCAAGTGCATCACGGGTTGCTAAGGCTGTTGGTATTGATGAGGTCTATTCTGGTCTTAAGCCTGAGGATAAGCTCCATCAAGTAAAATCTCTGTCACGCAATGGGGCTGGGGGTTTAATTATGGTGGGTGATGGAATAAATGATGCACCAGCTTTGGCTGCTGCAACTGTGGGGATTGTTCTTGCACAACGTGCAAGTGCAACGGCGGTGGCTGTAGCAGATGTTCTTCTCTTAGAGGATAATATCAACAGTGTACCTTTCATTATTGCAAAGGCACGTCAAACAACTTCCTTGGTAAAGCAGAGCGTAGCTGCTTGCATTATCATG

Fln Alignment:

Contig10600___TGWANLADALRENFRLCCSSMV----LLLLAAVCPYVM-----------PKTASRSAQNALIALAFPLVXXXXXXXXXXXXXXGRVNIHVLMTLAAFASVF---------------MGNSLEGGLLLAMFNLAHIAEEYYTK-RAMGDVKALKESNPEFALVLDAFDSAVPHQFSSMASKRVPVHDLEVGSYILVRAGEAVPVDGEVCQGVSMVTVEHLTGEANPLEKQVGDSVPGGARNLDGMLIIKATKTWKDSTLSKIMHLTEEAQLNKPKLQRWLDEFGEHYSEXXXXXXXXXAL---FGPFLFKWPFIGTPGARG----SIYRALGLMVAASPCXXXXXXXXXXXAISAC-ASKGILLKGGHALDALASCDTIAFDKTGTLTTGGLVCKGIEPIHGHSLLGRNDKNVNACCNPSCENEALAVASAMEKGTTHPIARAVADHSEGKEL---PP----------------VSIEHFESLPGKGLVATLTG---------IQSGDGVPLKATLGSVEYISSLFRNEFESRKIKEAASASPYGQDLVHAVLSVNNKVTLFHFEDKIRPGATDVVSTLKNKAGLRLMMLTGDHASSASRVAKAVGIDEVYSGLKPEDKLHQVKSLSRNGAGGLIMVGDGINDAPALAAATVGI
B8LQ20_______________TYFSSRMDESSQMFRLFTSSLTFSVPILFIGVVCPHIPFMYSLLLLRCGPFLMGDWLKWALVSPVQFIIGKRFYVAAYRALRNGSANMDVLIALGTSAAYFYSVCALIYGAVFHYRLATYFETSAMLITFVLLGKYLEVVAKGKTSNAIKKLLELAPTTALLLIT-DSDGKH----VEEKEIDAQLIQRSDMLKVYPGSKVPADGTVVWGSSHVNESMITGESALVSKEVGGTVIGGTLNLNGALHIQATKVGSDAALSQIVRLVETAQMAKAPIQKFADYIASVFVPVVVALAFVTWLGWYLAGVLGAYPEEWLPPASNHFVFALMFAISVLVIACPCALGLATPTAVMVATGIGATHGVLIKGGDALERAQKIQCVVFDKTGTLT------KGKPSVTSAKVLSDMQR-----------GEFLRIVASAEASSEHPLARAVVDYAYHFHFFGEPPKDMDMQGLRTKNTAWLLTASKFEALPGKGVRCSIEGTEILVGNRKLMSEDGVFIPSV--AEEYLKDMEQH-------ARTGILVAFDKELV-GMLAIS---------DPLKREAAVVIEGLK-KMGILPIMVTGDNWTTARAVAQELGIEDVKAEVIPAGKAEVIRSLQKDGT-MVAMVGDGINDSPALAAADVGM

SNPs (tot: 42)

Position	A %	C %	G %	T %	Probability
536	66	33			0.997452
539	66			33	0.997452
810		66		33	1.0
1215		83		16	0.994512
1220	22		77		0.999999
1224	77			22	0.999999
1309		83	16		0.994512
1312		16		83	0.994512
1313	16	83			0.994512
1314			16	83	0.994512
1319		80	20		0.995493
1321		20		80	0.995493
1322	20		80		0.995493
1324			20	80	0.995493
1325	20			80	0.995493
1327			20	80	0.995493
1344		25		75	0.996473
1364		25		75	0.996473
1464	75		25		1.0
1471	14		85		0.993533
1472	85	14			0.993533
1474			85	14	0.993533
1475		14		14	0.984337
1704	10		90		0.90298
1772			60	40	1.0
1858	10		90		0.90298
1859	90	10			0.90298
1861			90	10	0.90298
1862	90			10	0.90298
2412	42		57		1.0
2418	85			14	0.993533
2421		85		14	0.993533
2422			14	85	0.993533
2426		16		83	0.994512
2428	16			83	0.994512
2430			16	83	0.994512
2432		20	80		0.995493
2433	20			80	0.995493
2435	80		20		0.995493
2436	20			80	0.995493
2438		80	20		0.995493
2474	33	66			0.997452

SSRs (tot: 1)

Start position	End position	Sequence	Length
2039	2050	TGA TGA TGA TGA	12

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain