SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene10524

Length: 1567 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene10524

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	DUF604 multi-domain protein	-	-	73%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	63%
Blast2go	uncharacterized protein	-	-	89%

Source	GOs	Term	Type	e value	Identity
Blast2go	transferase activity, transferring glycosyl groups	GO:0016757	Molecular Function	0.0	89%

Source	InterPros	Term	Type	Identity
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Serine-threonine/tyrosine-protein kinase catalytic domain	IPR001245	-	-
Sma3	Homeodomain	IPR001356	-	-
Sma3	Gnk2-homologous domain	IPR002902	-	-
Sma3	Fringe-like	IPR003378	-	-
Sma3	POX	IPR006563	-	-
Sma3	Protein of unknown function DUF604	IPR006740	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3		IPR012287	-	-
Sma3	Homeobox, conserved site	IPR017970	-	-
Sma3	DNA methylase, N-4 cytosine-specific, conserved site	IPR017985	-	-

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LNL2

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 91 aas, putative chimeric sequence, your sequence is shorter than subject: 241 - 492

Fln protein: VVTLAVTQFVMGDDDTVFVLDNLLTVLSKYDHRQMYYIGSLSESHLQNIYFSYNMAYGGGGFAISYPLAKALESMQDGCIQRYPGLFASDDRIQACMAELGIPLTKEPGFHQFDIYGNLFGLLAAHPITPLVSLHHLDIAEPIFPSRTRVEALQHLFKAVKVDSAGTLQQSICYDQKREWSFTVSWGYAVQIFRGIFSPRELEMPSRTFLNWYRRADFTAYAFNTRPVTRHPCQKPFVFT*

Protein Length: 242

Fln nts: CGTCGTGACCTTGGCTGTCACTCAGTTTGTCATGGGAGATGATGACACAGTGTTTGTGCTTGACAATTTGTTGACTGTTCTGTCAAAGTATGATCACAGACAGATGTACTACATTGGCAGCCTGTCTGAAAGTCACCTGCAGAATATATATTTTTCATATAATATGGCTTATGGAGGTGGTGGGTTTGCCATCAGCTATCCATTGGCCAAAGCATTGGAATCAATGCAGGATGGTTGCATTCAGAGGTATCCGGGCTTGTTTGCAAGTGATGACAGAATACAAGCTTGCATGGCGGAGTTGGGTATACCATTGACGAAGGAGCCTGGTTTTCACCAATTTGATATATACGGTAACCTCTTTGGATTGCTTGCTGCACATCCTATCACGCCATTGGTATCTCTTCACCATCTAGATATTGCGGAACCCATCTTTCCGAGCCGCACAAGAGTAGAAGCTCTGCAACATCTCTTCAAGGCTGTAAAGGTAGATTCGGCAGGAACACTGCAGCAATCCATTTGCTATGATCAGAAAAGAGAATGGTCTTTTACAGTGTCATGGGGCTATGCTGTACAGATTTTTCGGGGCATCTTCTCTCCTAGAGAGCTCGAAATGCCCTCAAGAACTTTCTTGAATTGGTATAGAAGAGCTGATTTCACTGCCTATGCTTTCAATACCAGGCCTGTCACCAGGCATCCATGCCAGAAGCCCTTCGTTTTTACATGA___AGAATATTAAATATGACAATTCTACGAAAATTACTTTAAGCAATTATGTTCGAGACGAGGTTAAACCGCCTGAATGCAGATGGAGAATGGCTTCACCTGAGACAATAGAACGGATCAGGGTTTTCAAGAAGCCTCAGACAGTTTCAATACGACAGGCACCTCGACGAAACTGCTGTAGAGTAATAGCATCAAGGAAGGGGAACATCGACATTGAAGTGGGTAAGTGCAAGGAAGGTGAGATCAGTGGGTTGTAGACTTGCAGTAGCTGGCCACAGCAGACCTTCCATGAGGCTTGTGGAAGGACGGGCATTGTGATATTAGAAAATGAACGCATTATAGAATAATGGAAGGTCAATCTTTGCAACCATATGCTGGATACCATTTACATCACTTTGGTCTGAGGTAGTAGAGATTGAACTATAACCTGAGCGCTGGGAGATATTCCACTGGTATTATTGTAATCTCTCATTGGTATTATTGTTACCCTAATGAGAAGAACAAACAGGAGAAGGAATTCAAAGGGAGAGAGAAAAGAGGTTGGCAGAGAGATTTCTACTTCTACTTATTGGCCCTGTATGGCCATGTATTCATTGTTCTTTCTATTGATGAAGTTCATCAGTGAGGTCATTATTTAATTCTATTCTTCATTACCACTATTCTTCATTACCAGTTTCATTTATGAAACATGTTGGACATGTTTCCTCACTTTTTTCCCCTGTTACCTATTCTAATGAAATAAGTTGGCCCAGGCTAGGGTCTACATGAAGAATCTTTTCCCTATGTTGTTTACCTATTCTAATGAAATTAAATTGGCCCAGGCTAGGGTCTACATGAAGCATGACT

Fln Alignment:

Contig10524___VTQFVMGDDDTVFVLDNLLTVLSKYDHRQMYYIGSLSESHLQNIYFSYNMAYGGGGFAISYPLAKALESMQDGCIQRYPGLFASDDRIQACMAELGIPLTKEPGFHQFDIYGNLFGLLAAHPITPLVSLHHLDIAEPIFPSRTRVEALQHLFKAVKVDSAGTLQQSICYDQKREWSFTVSWGYAVQIFRGIFSPRELEMPSRTFLNWYRRADFTAYAFNTRPVTRHPCQKPFV
B8LNL2_______________VRWFVMGDDDTLFLPQNLVRVLAKYDHNQYYYIGSNSESTDQNAMHSYGMAYGGGGFAISYPLARDLAQVQDDCLHRYPHLYGSDQRVQACLAELGVPLTKEPGFHQFDIHGNLFGLLAAHPIAPLVSLHHLESVAPVFPNMTRVGALRHLMEAARVDPDRALQQSICYDARRKWTVSVSWGYCVQVLDRIELPRVLEFPLQTFVAWGHSMK-TPFLFNTRPVPRNPCQRATV

SNPs (tot: 41)

Position	A %	C %	G %	T %	Probability
458	20			80	0.995493
459	20	80			0.995493
462		80		20	0.995493
950	86	13			0.999973
957	86	13			0.999973
958		13	86		0.999973
959			86	13	0.999973
960	86			13	0.999973
961	86		13		0.999973
963		13	86		0.999973
966	86			13	0.999973
967		13	86		0.999973
1061	81		18		1.0
1063		9	4	85	0.999726
1064	80		14	4	1.0
1066	85	4			0.999726
1069	80	14		4	1.0
1070		9		90	0.999872
1071	85		4	9	0.999726
1072	85	4			0.999726
1076	85	14			1.0
1077	90			9	0.999872
1079			85	5	0.999793
1083	90	10			0.999898
1103		20	79		1.0
1105	79			20	1.0
1106			20	79	1.0
1113			20	79	1.0
1114	79		20		1.0
1116	79			20	1.0
1123		16		83	1.0
1124	8			91	0.999766
1175	2	91	5		0.99441
1176	94			5	0.998578
1179		5	94		0.9982
1180	7		92		1.0
1181		5		94	0.9982
1182	92	7			1.0
1185	92		7		1.0
1186	2		5	92	0.992495
1190	92	5		2	0.992495

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain