SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene10490

Length: 1582 nt

Origin of reads: Biogeco1 v2 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene10490 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Unassigned protein	-	-	-
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	81%
Blast2go	uncharacterized protein	-	-	76%

Source	GOs	Term	Type	e value	Identity
Blast2go	cytoplasmic membrane-bounded vesicle	GO:0016023	Cellular Component	0.0	76%

Source	InterPros	Term	Type	e value	Identity
Sma3	Ureohydrolase	IPR006035	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NTD2

Fln msg: Overlapping hits, possible frame ERROR between 680 and 673,

Fln protein: MARFMVNCRAFLMLSVLHFHPFFPPIEGRSLVSPGAGFRLRPTHRTPLSIVGFCAKASGATAKDVDPAKIAANLVALLGSEEEARSVDPDLAQELLSCLRFLVPRVESWRxxxxSRNGEISDGNVETRRRFESRKWLAVAQDERVKELIWWPPAPVMELARSAVEWGGDHSVIERALNSTHYPVPDVEQCIEHKCQLTRTTFGRRFVNEELNLYMAFLFETISSLASSAGFNVSLNRYDLFHGHLFLATNTGRLGILFHAKEYPAYNKETFPFHLGYCQTGSFVAYDSNMNLRNILWLAPMPDFSNRKCSSQWLAPGALVVLDAHPGGIIYEDLVGQWVQDVRTIYEDDFGDVIFDVNYLNVANSVSENKIFIC*

Protein Length: 376

Fln nts: ATGGGGATGGGGATCGCCATTGAGAGCGTTTCTGTGTAAAATGGATGTTGAGTTTGCATCAAAATAATTTATCCCATTGAGAGCGTTTCTGTGTAAAATTGATGTTGAGTTTGCATCAAAATAATTCATCCTTTGCCCCCAGAAACCTGCAGAGCTCCTCTGCCATCCACATGAGTTGCATAAGGTTTTGCGTAAACATGTGACAAAACTCTCTTAAATAATTAATGTAAAAAGTATGCCACACGCTAAGGAGAGAGCAAGCAATGGAAGTAAACCAGAGATTTAAAGACGAGAAAGATTTGGAGTATCGATGAAAGTATTTTGCAAAGGGAGTCAGTCGG_-_ATGGCCAGATTTATGGTCAATTGCCGTGCTTTCCTCATGCTCTCCGTTCTGCATTTCCATCCATTCTTTCCGCCTATAGAAGGAAGATCTCTGGTCTCTCCCGGTGCAGGATTCCGACTTCGCCCAACGCATAGAACCCCACTAAGTATAGTGGGATTTTGCGCCAAGGCATCCGGAGCGACAGCCAAAGACGTCGATCCTGCTAAAATCGCCGCCAATTTGGTAGCCCTTCTGGGAAGCGAGGAGGAGGCTCGTTCAGTGGACCCGGATTTAGCTCAGGAGCTACTCAGCTGTCTCCGGTTCCTGGTACCTAGGGTTGAATCTTGGCGAxxxxxxxxxxxxTCCCGCAACGGGGAAATTAGCGATGGAAATGTGGAGACCCGGAGAAGGTTCGAATCCCGGAAGTGGCTGGCTGTAGCACAGGACGAGAGGGTGAAAGAGCTTATCTGGTGGCCTCCGGCACCCGTGATGGAGCTTGCAAGGAGCGCGGTTGAATGGGGAGGCGATCATTCTGTAATTGAACGAGCGCTAAATTCAACTCATTATCCGGTGCCGGATGTGGAACAATGTATAGAGCATAAGTGTCAACTGACCAGAACCACTTTTGGGCGACGCTTTGTAAATGAGGAATTAAACTTATACATGGCCTTTCTATTTGAGACTATTTCTTCCCTTGCATCCTCAGCTGGATTTAATGTATCATTGAACCGCTATGACTTATTCCATGGACATCTGTTTCTTGCTACCAACACTGGGAGACTTGGGATCTTGTTTCATGCCAAAGAATACCCAGCATACAACAAAGAAACTTTTCCATTTCATCTTGGCTACTGCCAAACAGGCTCCTTTGTGGCATATGACAGCAACATGAATCTACGAAATATTTTATGGTTGGCACCAATGCCAGATTTTTCTAACAGAAAATGTTCATCTCAATGGTTAGCCCCAGGTGCCTTGGTGGTCCTTGATGCTCACCCCGGGGGAATAATATATGAAGATTTAGTTGGTCAGTGGGTTCAGGATGTCAGGACTATTTATGAAGATGATTTTGGGGATGTGATATTTGATGTGAACTATTTGAATGTGGCAAACAGTGTATCAGAAAACAAGATCTTTATTTGTTGA___ACATCTAATTGCGTCAAGGCACATTTTATAGCTCAGTATGAGGGAGATGTGTAATTCCTTCCATACTAAATATCAAGTTGACATATGTTCCTTTTAAAATAAAAATAAAATATTCTCT

Fln Alignment:

Contig10490___MARFMVNCRAFLMLSVLHFHPFFPPIEGRSLVSPGAGFRLRPTHRTPLSIVGFCAKASGATAKDVDPAKIAANLVALLGSEEEARSVDPDLAQELLSCLRFLVPRVESWRxxxSRNGEISDGNVETRRRFESRKWLAVAQDERVKELIWWPPAPVMELARSAVEWGGDHSVIERALNSTHYPVPDVEQCIEHKCQLTRTTFGRRFVNEELNLYMAFLFETISSLASSAGFNVSLNRYDLFHGHLFLATNTGRLGILFHAKEYPAYNKETFPFHLGYCQTGSFVAYDSNMNLRNILWLAPMPDFSNRKCSSQWLAPGALVVLDAHPGGIIYEDLVGQWVQDVRTIYEDDFGDVIFDVNYLNVANSVSENKIFIC
A9NTD2_______________MAGFKVTCRAFFMLSALHFHPFFPSTEGRSLVSHGTRFLLRRTHRVPLSIVGISTKASAATAKDVDPAKIAANLVALIGSEEEARSVDPDLAEELLSCLRFLVPRVVSRRxxxSRDVKISYGIEETRQGFESPKWMAAVQDESVNNLIWWPPTSVMELARSAVEWGGDHSVIERALNSIHYPVPDIEHCIEHKCQLTRTTFGRRFVNEELNLYMAFLFETISSLAPSAGFNVSLNRYDLFHGHLFLATNTGRLGILFHAKEYPAYNKETFPFHLGYCQTGSFVAYDCSMNLRNILWLAPLPDCSNRKCSSQWLAPGALVVLDAQPGGIIFEDLVGQWVQDVRTIYEDDFGDVIFDVNYLNVANSASENKIFLC

SNPs (tot: 62)

Position	A %	C %	G %	T %	Probability
360	90			10	0.90298
361			10	90	0.90298
364			10	90	0.90298
365	10			90	0.90298
368		10		90	0.90298
370	10		90		0.90298
371	90		10		0.90298
372			10	90	0.90298
376		10		90	0.90298
378	10			90	0.90298
379		10		90	0.90298
406	11			88	0.923564
407		11		88	0.923564
408	11			88	0.923564
410		11	88		0.923564
414	11			88	0.923564
415	88		11		0.923564
417		88		11	0.923564
420	88	11			0.923564
423		11		88	0.923564
491	77			22	0.999999
493	22			77	0.999999
499	22		77		0.999999
500		22		77	0.999999
509	77			22	0.999999
510	77	22			0.999999
516		20		80	0.999996
587		80	20		0.995493
588	20			80	0.995493
589		80	20		0.995493
590	80			20	0.995493
591	80		20		0.995493
593		20		80	0.995493
596	80	20			0.995493
601		80		20	0.995493
604		20		80	0.995493
606	40			60	1.0
607	60	20	20		0.99255
608			20	80	0.995493
927		33		66	0.997452
980		75	25		0.996473
1023	80		20		0.995493
1106		16		83	0.994512
1111		16		83	0.994512
1132	16		83		0.994512
1255		83	16		0.994512
1257	25	75			0.996473
1469	20			80	0.995493
1527			20	80	0.995493
1529			20	80	0.995493
1530	20			80	0.995493
1531		20	80		0.995493
1533			20	80	0.995493
1534		20	80		0.995493
1538	80		20		0.995493
1539		80	20		0.995493
1545	80		20		0.995493
1546	20			80	0.995493
1548		80	20		0.995493
1579	33	66			0.997452
1580	33	66			0.997452
1581		66	33		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain