SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene10295

Length: 1748 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene10295 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Mitochondrial substrate carrier family protein [Arabidopsis thaliana] emb\|CAB87921.1\| peroxisomal Ca-dependent solute carrier-like protein [Arabidopsis thaliana] dbj\|BAD42991.1\| peroxisomal Ca-dependent solute carrier-like protein [Arabidopsis thaliana] g	-	-	9.99967e-42	77%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	89%
Blast2go	mitochondrial substrate carrier family protein	-	-	0.0	87%

Source	GOs	Term	Type	Identity
Blast2go	transmembrane transport	GO:0055085	Biological Process	87%
Blast2go	calcium ion binding	GO:0005509	Molecular Function	87%
Blast2go	mitochondrial inner membrane	GO:0005743	Cellular Component	87%
Blast2go	integral to membrane	GO:0016021	Cellular Component	87%

Source	InterPros	Term	Type	Identity
Sma3	Enolpyruvate transferase domain	IPR001986	-	-
Sma3		IPR001993	-	-
Sma3	Calcium-binding EF-hand	IPR002048	-	-
Sma3	Mitochondrial carrier protein	IPR002067	-	-
Sma3	Adenine nucleotide translocator 1	IPR002113	-	-
Sma3	EF-hand-like domain	IPR011992	-	-
Sma3	Mitochondrial substrate/solute carrier	IPR018108	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-
Sma3	EF-hand	IPR018248	-	-
Sma3	EF-HAND 2	IPR018249	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: A9NX45

Fln msg: Overlapping hits, possible frame ERROR between 1191 and 1191, and possible frame ERROR between 1457 and 1458, your sequence is shorter than subject: 250 - 511. Was predicted as Complete, but is very much shorter than de subject

Fln protein: MSGAGQAVDLAPFSKMESMPKTQRDNRRAECCNPVRNTGEVSLEHVMLALRETKEERELRIRSLFxxFDAMNSGHLDNSQIERGLQALRIPAEYKYAKDLLQVCDSNQDGRVEYQEFRRYMDDKELELYRIFQAIDVEHNGCILPEELWEALVRADPGPVVQLGCGTVSGALGATCVYPLQLIRTRLQAQPMNSPSRYKGMSDVFWRTLQNEGFRGFYKGLFPNLLKVAPAASITYLVYEKMKKVLQLD*

Protein Length: 251

Fln nts: ATGGGGCCACTCAAGTCCATATGGGCTCGGCAGAGGACGGAGCAGGGATATATTCAATGAATATATCCCCTCTCGGGAAGCTTGGATCCGGCGAGTTATGCGCGTCGGGTTCCTGTTCCTTGCAGAGGCGGATTGATTTCCCGTCATCAGACGAAGCACATATGCCTCTGGACTGATATGCCGATCTACTGACTGGAATTAGGTATGAGAAATTAACCGAATATTCCACGAAGCGTCCCGGGGCCCCGTAGAAGATTCCATCCCCGGACGCTGCACGACTTCGGGAATTCAAAGTCTTTTTCGTCAGTCAAATTGCGGAAGTAGATCTCACGCGTGGACTTGAAGGAGGGAATATATGTTCCCCAGTCTGTCTGTTTGTCTATTGCTCTGAAGAAAGAAAGCAGACAAGCAATTCTGTGAGTTAATTCAGCTGAGAAGAGATTCAGAAAATATTTGCCTTCCAGTGAAATAAAGATACAGGGTCCAATTTTCATGGTTGTGAATTAGTGATATAAGGTTTGATCGGCCCTACGATTTAAATTTCCGAGCGAATATGAGTTCTCTTGGTTGTGTGCGGTGACTGGAGTATGTAGGGTTTGATCGGCCCTACGATTTCAATTTCCGAACAAGTTTATTATTATTTTTTTCTGGTAGTTCGTATTGAATTAAAGATACGAGGTTTGATCGGCCCCATATTTCATCTTCTTGGCAAATTTTGAGCATATCTCGTGAATTAAAGACATGGGCCCTAGGATTTGAATTTCCGAGCCAATTAAGTCTTTTCTGGGCTGTCTGTAGTTAATTAAAGGTGCATGGATTGATCCGTCCGGGAGATTAATTTCTGAGGATATTTGAGCATTTCTGGCTGTCTGGTGAAGTAAATAGATAGGGTTTGATCAACCAGAGGATTTCAATTTCCGAGCAAATCTGAAGCATTTCTTGTTGCAAGTCGTCGCAATTTCCGTAATTGCAAGCATCGAAGCTCGAAAATTCATGTCGGGCGCTGGCCAGGCCGTTGACCTTGCTCCCTTCTCCAAAATGGAATCTATGCCAAAGACACAGCGGGATAATCGTCGGGCCGAATGTTGCAATCCTGTGAGAAATACAGGCGAGGTGTCCTTAGAGCATGTTATGTTAGCTTTGAGGGAGACGAAAGAGGAGAGGGAATTGAGGATCCGGAGTCTGTTTxxxxxxTTTGATGCAATGAACAGTGGGCACCTTGACAATTCGCAAATTGAGAGGGGTCTACAGGCATTGCGGATACCGGCCGAATACAAGTATGCCAAGGATCTGCTTCAAGTTTGCGATTCTAATCAGGATGGCCGTGTCGAGTACCAGGAGTTTCGGCGGTATATGGATGACAAGGAACTCGAGCTTTACCGCATTTTTCAGGCGATAGATGTGGAACACAATGGCTGCATTTTACCTGAAGAGCTATGGGAAGCACTCGTTAGAGCAGACCCAGGTCCTGTTGTGCAACTAGGATGTGGAACAGTTTCAGGGGCACTTGGAGCAACATGTGTTTATCCTTTGCAACTTATCAGAACACGATTACAAGCTCAACCTATGAATTCACCTTCCAGATACAAGGGCATGTCTGATGTTTTTTGGAGAACCCTCCAGAACGAAGGGTTTAGAGGTTTCTACAAGGGCCTTTTTCCAAATTTGCTGAAAGTGGCTCCAGCAGCTAGTATTACTTATTTAGTCTATGAGAAAATGAAAAAGGTGCTCCAGCTAGATTGAAGAACA

Fln Alignment:

Contig10295___MSGAGQAVDLAPFSKMESMPKTQRDNRRAECCNPVRNTGEVSLEHVMLALRETKEERELRIRSLFNFFDAMNSGHLDNSQIERGLQALRIPAEYKYAKDLLQVCDSNQDGRVEYQEFRRYMDDKELELYRIFQAIDVEHNGCILPEELWEALVRADPGPVVQLGCGTVSGALGATCVYPLQLIRTRLQAQPMNSPSRYKGMSDVFWRTLQNEGFRGFYKGLFPNLLKVAPAASITYLVYEKMKKVLQLD
A9NX45_______________MSGAGQAVDQIPFSKMESIPKTQQDNRRAECCNPVRNSGEASLEHVMLALRETKEERELRIRCLFNFFDAMNSGHLDNSQIERGLQALRIPAEYKYAKDLLNVCDSNQDGRVEYQEFRRYMDDKELELYRIFQAIDVEHNGCILPEELWEALVKADPGPIVQLGCGTVSGALGATCVYPLQLIRTRLQAQSMNSPSRYKGMSDVFWKTLQHEGFSGFYKGLFPNLLKVAPAASITYLVYEKMKKVLQLD

SNPs (tot: 6)

Position	A %	C %	G %	T %	Probability
1304			25	75	0.996473
1322		25	75		0.996473
1323	25	75			0.996473
1325			75	25	0.996473
1376		66	33		0.997452
1574		66		33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain