SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene10150

Length: 1175 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene10150 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	diacylglycerol kinase [Arabidopsis thaliana]	-	-	74%
FL-Next	sp=Diacylglycerol kinase 1; Arabidopsis thaliana (Mouse-ear cress).	-	-	78%
Blast2go	diacylglycerol kinase	-	-	84%

Source	ECs	Term	Type	e value	Identity
Blast2go	Diacylglycerol kinase.	EC:2.7.1.107	-	0.0	84%

Source	KEGGs	Term	Identity
Blast2go	Glycerolipid metabolism	00561	84%
Blast2go	Glycerophospholipid metabolism	00564	84%
Blast2go	Metabolic pathways	01100	84%
Blast2go	Phosphatidylinositol signaling system	04070	84%

Source	GOs	Term	Type	Identity
Blast2go	root development	GO:0048364	Biological Process	84%
Blast2go	response to cold	GO:0009409	Biological Process	84%
Blast2go	response to wounding	GO:0009611	Biological Process	84%
Blast2go	leaf development	GO:0048366	Biological Process	84%
Blast2go	activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway	GO:0007205	Biological Process	84%
Blast2go	diacylglycerol kinase activity	GO:0004143	Molecular Function	84%
Blast2go	calcium ion binding	GO:0005509	Molecular Function	84%
Blast2go	protein binding	GO:0005515	Molecular Function	84%
Blast2go	intracellular signal transduction	GO:0035556	Biological Process	84%

Source	InterPros	Term	Type	Identity
Sma3	Diacylglycerol kinase, accessory domain	IPR000756	-	-
Sma3	Diacylglycerol kinase, catalytic domain	IPR001206	-	-
Sma3	Protein kinase C-like, phorbol ester/diacylglycerol binding	IPR002219	-	-
Sma3	Beta tubulin, autoregulation binding site	IPR013838	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: sp_plants

Fln subject: Q39017

Fln msg: Separated hits, possible frame ERROR between 168 and 185, your sequence is shorter than subject: 186 - 728

Fln protein: RRDLGCHSIMDRTCADLPWKIRLEVDGVDIEVPEDSQGILVTNIGSYMGGVDLWQxxxxxxxDFDPQSMHDKVLEVVSICGTWHLGKLQVGLSRARRLAQGQLIRIHTFVSAFPVQIDGEPWIQQPCSLEIAHHGQAFMLKRASEEPFGHAAAIMAEVLENAVCKGVINAAQKRALLQEMALRLS*

Protein Length: 187

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATTATGGATAGAACATGTGCAGACCTTCCTTGGAAAATTCGTTTAGAAGTGGATGGTGTGGATATAGAAGTTCCCGAGGACTCACAGGGCATCCTTGTAACAAATATTGGGAGCTATATGGGAGGTGTAGATCTGTGGCAAAAxxxxxxxxxxxxxxxxxxTGATTTTGATCCACAATCCATGCATGATAAGGTGCTTGAAGTTGTCAGTATTTGTGGAACATGGCATCTTGGAAAGCTCCAGGTGGGGCTTTCTCGTGCTCGAAGGCTTGCCCAAGGGCAGTTGATTAGGATCCATACATTTGTTTCTGCTTTTCCTGTACAGATTGATGGCGAACCATGGATTCAACAACCTTGCAGTCTAGAAATAGCACATCATGGACAGGCTTTCATGTTAAAGAGGGCTTCTGAGGAGCCCTTCGGCCATGCTGCAGCTATTATGGCTGAGGTGCTTGAGAATGCAGTATGTAAGGGGGTGATTAATGCTGCCCAAAAAAGAGCTCTCCTTCAAGAAATGGCTTTACGTTTGTCGTAGGATATGTACAGAAGGGGTGCTGATATAATTTCCTTCCAGTGTTGCTCTGTCTAGCGTGGAATTTCAAAATTATTTTGATAGATCTCTTTCACATTTTGGCAGGAAATGAATTAGCTGGCTCAGTATCAGTGTTTTTGGGATTTTGCAACTTGTGATGTGAGCAATTCCTTTCAGCATGATTGTTTATACTGTTTCAATTGGAAATAGACTAAACAATAATGATGACTGTAAGCGCTAATCTGGCAATACTGGTGCCTCTTGCGGCTTTATAGATTAATTGTATTTAGGCTGCTTATTACAAGTAAGACTTTGATCCACTTTTGGAGGCCACCGCTTGGAGCTGATCCACTGTTGGAGGCCACCGCTTTAAACAACTACTGTGCTCATATATATTTGTAACGCGTCTTTCATTTATCAGATAATGGAGACAAGCTCATCTTTCGTTAATCATTTACAGACTGATGTATTGTATCCAACCTAATCTCTAGAGAAGAGAATGAGCATTCTACATTTTTTCTGGAGCAATAAGCAATTATGTATTTTCAAAGTTTCTTTCAATCATGACACTAATTTCTATTGCTAACGCACTGCAAGACTCAGGTGACCTTTTCTGTATTGC

Fln Alignment:

Contig10150___GCHSIMDRTCADLPWKIRLEVDGVDIEVPEDSQGILVTNIGSYMGGVDLWQNxxxxxxDFDPQSMHDKVLEVVSICGTWHLGKLQVGLSRARRLAQGQLIRIHTFVSAFPVQIDGEPWIQQPCSLEIAHHGQAFMLKRASEEPFGHAAAIMAEVLENAVCKGVINAAQKRALLQEMALRLS
Q39017_______________GARSIMDRTFEDFPWQVRVEVDGVDIEVPEDAEGILVANIGSYMGGVDLWQNxxxxxxNFDPQSMHDKIVEVVSISGTWHLGKLQVGLSRARRLAQGSAVKIQ-LCAPLPVQIDGEPWNQQPCTLTISHHGQAFMLKRAAEEPLGHAAAIITDVLENAETNQVINASQKRTLLQEMALRLT

SNPs (tot: 31)

Position	A %	C %	G %	T %	Probability
614			84	15	0.999986
615			15	84	0.999986
617		84	15		0.999986
618	76	7		15	0.999983
619		7	15	76	0.999983
622			15	84	0.999986
625	16			83	0.999991
627		83	16		0.999991
629	83			16	0.999991
632	75	25			1.0
736		85		14	0.993533
743			85	14	0.993533
744		85	14		0.993533
745	14			85	0.993533
746		85	14		0.993533
749		85	14		0.993533
751		14	85		0.993533
757	14	85			0.993533
758	14			85	0.993533
759		85	14		0.993533
760			20	80	0.999996
762		11		88	0.923564
764	88	11			0.923564
765		88	11		0.923564
767		11		88	0.923564
840	88	11			0.923564
841		12	87		0.992554
842		12	87		0.992554
844	87			12	0.992554
929	75			25	0.996473
930			75	25	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain