SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene9896

Length: 1627 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene9896

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Pectinesterase n=1 Tax=Picea sitchensis RepID=A9NUI7_PICSI	-	-	67%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	86%
Blast2go	pectin methylesterase	-	-	70%

Source	ECs	Term	Type	e value	Identity
Blast2go	Pectinesterase.	EC:3.1.1.11	-	0.0	70%

Source	KEGGs	Term	Identity
Blast2go	Pentose and glucuronate interconversions	00040	70%
Blast2go	Starch and sucrose metabolism	00500	70%
Blast2go	Metabolic pathways	01100	70%

Source	GOs	Term	Type	Identity
Blast2go	cell wall modification	GO:0042545	Biological Process	70%
Blast2go	negative regulation of catalytic activity	GO:0043086	Biological Process	70%
Blast2go	pectinesterase activity	GO:0030599	Molecular Function	70%
Blast2go	aspartyl esterase activity	GO:0045330	Molecular Function	70%
Blast2go	enzyme inhibitor activity	GO:0004857	Molecular Function	70%
Blast2go	plasma membrane	GO:0005886	Cellular Component	70%
Blast2go	cell wall	GO:0005618	Cellular Component	70%

Source	InterPros	Term	Type	Identity
Sma3	Pectinesterase, catalytic	IPR000070	-	-
Sma3	Ribosome-binding factor A	IPR000238	-	-
Sma3	Aminoacyl-tRNA synthetase, class I, conserved site	IPR001412	-	-
Sma3	Metallophosphoesterase domain	IPR004843	-	-
Sma3	Pectinesterase inhibitor	IPR006501	-	-
Sma3	Carbohydrate-binding/sugar hydrolysis domain	IPR006633	-	-
Sma3	Pectin lyase fold	IPR012334	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3	Pectinesterase, active site	IPR018040	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: D5ADI1

Fln msg: Overlapping hits, possible frame ERROR between 359 and 356, your sequence is shorter than subject: 511 - 553

Fln protein: CRATQLPDLCKSSLSTKVAANTGAEEIIRAAMGLATDGAKQTHLLSQNLLATSTDNANVSAAAKNCVEFADYSIALIAKGSAAIGVEQRIKDVKAWLSAALAYVYDCSSDLRYVNTTxxxSTLIQQVDRVTNLTSNALSMVDALDTYGKDMVLWRPPSTERSPKSSNLPTNYYYLPNNNNWDWDVSEVNELIPDITVSKVDQKFSLSSIQQAVNSAPDYSEKKFVIKIQAGVYGETVRIPRSKTNLVFVGAGMDKTVITGSAYVPSLPGPVTIYDVATVGVNGDGFIARDITFRNTFQGPQTHQAVALRVDSDFSAFYNCAFESHQDTLYTHTLRQFYRNCRIEGTHDFIFGNAAALFHNCSILVRPRQLKSKSGEKDPLTAQGRTDPAQSTGLVFQHCTVDGTKEYMRDFNSNPDIHRVYLGRPWKMYSRTVFLDSYLGKLIRPEGWMPWNGSFALETLFYGEYQNYGPGAQVSGRVPWSSQISAHNIGLYSVQSFIQGDEWLSSSHST*

Protein Length: 512

Fln nts: CCTGCCGTGCAACTCAATTGCCAGACCTATGCAAGTCATCTTTGTCTACCAAAGTAGCTGCAAATACCGGAGCTGAAGAGATCATTAGAGCAGCGATGGGTTTGGCAACAGATGGAGCTAAACAGACACATCTTCTCTCCCAGAATCTGCTTGCCACCTCTACCGATAATGCCAATGTCTCAGCAGCTGCTAAAAACTGCGTGGAATTCGCGGATTATTCCATCGCTCTGATTGCCAAAGGCAGTGCAGCTATAGGAGTAGAGCAAAGAATAAAAGATGTGAAAGCATGGCTGAGCGCAGCACTGGCTTATGTATACGACTGTAGCTCTGATCTCAGATATGTCAATACTACAxxxxxxxxxAGCACTCTCATCCAACAGGTGGATAGGGTTACAAACTTAACCAGCAATGCTCTGAGCATGGTCGATGCCCTGGATACTTACGGGAAGGATATGGTCCTATGGAGGCCTCCCTCAACGGAAAGATCACCCAAATCCTCTAATCTCCCCACAAATTATTACTATCTTCCCAATAACAACAACTGGGATTGGGATGTATCAGAGGTGAACGAACTGATCCCTGACATTACTGTGTCGAAGGTGGATCAGAAATTCTCTTTATCAAGCATTCAGCAAGCTGTGAACAGTGCGCCGGACTACTCGGAAAAGAAATTTGTGATCAAGATACAGGCCGGGGTTTACGGGGAAACGGTTCGAATCCCCCGCAGTAAGACGAATCTCGTGTTTGTGGGCGCCGGTATGGATAAAACGGTGATCACCGGTTCTGCATATGTGCCGTCTCTGCCCGGCCCGGTCACAATTTACGATGTCGCCACTGTTGGAGTGAATGGGGACGGCTTCATAGCCCGTGACATAACATTCCGAAACACATTTCAGGGGCCACAGACTCATCAAGCCGTGGCCCTGAGAGTAGACAGCGACTTTTCTGCCTTCTACAACTGCGCTTTCGAGAGCCACCAGGACACGCTCTACACGCACACGCTCCGCCAATTCTACAGAAACTGCAGAATAGAGGGCACCCACGACTTCATTTTCGGAAACGCGGCGGCCCTATTCCACAACTGTTCCATTCTGGTGCGCCCCCGTCAGCTCAAATCAAAGTCTGGCGAGAAGGATCCCTTAACAGCGCAGGGCCGGACGGACCCTGCTCAGTCAACCGGATTGGTCTTCCAGCACTGCACCGTGGATGGGACCAAAGAATACATGAGGGATTTCAATTCCAATCCCGATATTCACAGAGTATATCTTGGCAGACCATGGAAGATGTATTCAAGAACGGTTTTCTTGGATTCTTATTTGGGCAAACTCATCAGGCCTGAAGGGTGGATGCCCTGGAACGGATCCTTTGCTCTTGAAACTCTTTTCTATGGGGAATATCAGAATTATGGACCGGGGGCGCAGGTTTCTGGAAGGGTGCCATGGTCTAGTCAAATTTCTGCACACAATATAGGACTGTATTCTGTTCAAAGCTTCATACAGGGCGATGAATGGCTGTCATCGAGCCACAGTACATAAATAACCAGCGCAATTTTTAATGGTTATATGGTTTTTTCCATTTTCCAGTAATAAACACCGAGCCTTAGCGTAATGTTCATATCAAAAGCTTCA

Fln Alignment:

Contig9896___CRATQLPDLCKSSLSTKVAANTGAEEIIRAAMGLATDGAKQTHLLSQNLLATSTDNANVSAAAKNCVEFADYSIALIAKGSAAIGVEQRIKDVKAWLSAALAYVYDCSSDLRYVNTTQxISTLIQQVDRVTNLTSNALSMVDALDTYGKDMVLWRPPSTERSPKSSXXXXXXXXXXXXXXWDWDVSEVNELIPDITVSKVDQKFSLSSIQQAVNSAPDYSEKKFVIKIQAGVYGETVRIPRSKTNLVFVGAGMDKTVITGSAYVPSLPGPVTIYDVATVGVNGDGFIARDITFRNTFQGPQTHQAVALRVDSDFSAFYNCAFESHQDTLYTHTLRQFYRNCRIEGTHDFIFGNAAALFHNCSILVRPRQLKSKSGEKDPLTAQGRTDPAQSTGLVFQHCTVDGTKEYMRDFNSNPDIHRVYLGRPWKMYSRTVFLDSYLGKLIRPEGWMPWNGSFALETLFYGEYQNYGPGAQVSGRVPWSSQISAHNIGLYSVQSFIQGDEWL
D5ADI1______________CRATQLPDLCKSSLSSKVAANAGAEEIIGAAMGLASDGAKQTHLLSQNLLATSKYDANITAAAKNCVEFADSSLALILKSSAALGVEQRIKDVKAWMSAALAYVYDCSSDLRYVNTTHxISTIIQQVDWVTNFTSNALSMVDALDTYGKDMVLWRPPTTERSANPSSTAHYYRLPNKN--WDWDVLEVDELVPNITVSKLDHKSSISSIQQAVNSAPDYSEKRFVIKIEAGVYEERVRIPRSKTNLMFEGAGMDTTVITGSAYVPRLPGPVTIYDVATVGVNGDGFIARGITFRNTFLGPRTHQAVALRVDSDFSAFYSCAFESHQDTLYTHTLRQFYKNCRIEGTHDFIFGNAAALFHNCSILVRPRQLKSNSGEDDPITAQGRTDPAQSTGLVFQHCTVDGTKEYRKDFSSNPSAHKVYLGRPWKMYSRTVFLNSYLGKLVRPEGWMPWNGTFALDTLFYGEYRNYGFGAKVSGRVPWSNQISELNVGLYSVPSFIQGHEWL

SNPs (tot: 26)

Position	A %	C %	G %	T %	Probability
572	75		25		0.996473
1002		25	75		0.996473
1165		66	33		0.997452
1276	66		33		0.997452
1278	33	66			0.997452
1279	33		33		0.997753
1283	66		33		0.997452
1296		33	66		0.997452
1298	66			33	0.997452
1299		66		33	0.997452
1378	75			25	0.996473
1393	25		75		0.996473
1402	25		75		0.996473
1418			66	33	0.997452
1421	25	75			0.996473
1426		25		75	0.996473
1435	25		75		0.996473
1469	60		40		1.0
1480		40		60	1.0
1487		60	40		1.0
1504		60		40	1.0
1524	20		80		0.995493
1542	20	80			0.995493
1567	33		66		0.997452
1569	33			66	0.997452
1570	33			33	0.997753

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain