SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene9253

Length: 1656 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene9253 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Cycloartenol synthase n=2 Tax=Pinaceae RepID=Q9FQM1_ABIMA	-	-	72%
FL-Next	tr=Cycloartenol synthase; Abies magnifica (Red fir).	-	-	74%
Blast2go	cycloartenol synthase	-	-	79%

Source	ECs	Term	Type	e value	Identity
Blast2go	Cycloartenol synthase.	EC:5.4.99.8	-	0.0	79%

Source	KEGGs	Term	Identity
Blast2go	Steroid biosynthesis	00100	79%
Blast2go	Biosynthesis of terpenoids and steroids	01062	79%
Blast2go	Biosynthesis of plant hormones	01070	79%
Blast2go	Metabolic pathways	01100	79%
Blast2go	Biosynthesis of secondary metabolites	01110	79%

Source	GOs	Term	Type	e value	Identity
Blast2go	metabolic process	GO:0008152	Biological Process	0.0	79%
Blast2go	cycloartenol synthase activity	GO:0016871	Molecular Function	0.0	79%

Source	InterPros	Term	Type	Identity
Sma3	ArgE/DapE/ACY1/CPG2/YscS, conserved site	IPR001261	-	-
Sma3	Prenyltransferase/squalene oxidase	IPR001330	-	-
Sma3		IPR001993	-	-
Sma3	Terpene synthase, conserved site	IPR002365	-	-
Sma3	Mitochondrial substrate/solute carrier	IPR018108	-	-
Sma3	Squalene cyclase	IPR018333	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: Q9FQM1

Fln msg: your sequence is shorter than subject: 453 - 756

Fln protein: VTWKMPSPPQPLIQDIMWTTLHKVVEPMLGHWPGSMLRERALQTVMKHAHYDDESTRYIGMNPDSKVMNMLCCWIEDKNSEAFKKHLPRILDYLWIAEDGMKTQCHNGSQLWDAAFAIQALISADLLDECGPLLKKAHAFIEKSQVQENSPGEFSTWYRHMSKGAWTLSTRDHGWVVADCSAEALKAALELSELPENIVGKPLPQQRIFDCVNYLLSMQNTNGGYATYELTRSYNWLGNFNPAEILGEFCIDHSYVECTSSAIQALALFKKLHPQHRRKEIEQCISNGSRYIENMQRPDGSWYGSWGVCFTYGTWFGILGLKAAGKTYQNCSTIRKACDFLLSKQLPSGGWGESYIACQKQVYTNLGDGRSHIVNTAWAMLALICAGQALRDPKPLHRAAIVLVNSQMENGDFPQWELRGVGCGCFVMGYAAYHNIFPIWALAEYRRHVLQS*

Protein Length: 454

Fln nts: CGTCACATGGAAGATGCCTTCCCCTCCACAGCCTTTAATACAAGACATTATGTGGACAACTCTTCATAAAGTGGTAGAGCCTATGCTTGGGCATTGGCCAGGCTCAATGTTGCGGGAGAGAGCACTTCAGACGGTGATGAAGCATGCGCACTATGACGATGAAAGCACTCGCTACATTGGCATGAACCCTGATAGCAAAGTAATGAATATGCTATGTTGCTGGATAGAAGATAAAAACTCAGAGGCATTTAAAAAGCACCTTCCACGAATCCTAGACTATCTATGGATTGCAGAAGATGGCATGAAGACGCAGTGCCATAATGGCAGTCAGTTATGGGATGCAGCATTTGCTATCCAAGCTCTAATATCGGCCGACCTCCTTGACGAATGCGGTCCACTGCTTAAGAAAGCACACGCGTTTATAGAAAAGTCACAGGTTCAAGAAAATTCTCCCGGTGAGTTTAGTACCTGGTATCGCCACATGTCTAAGGGTGCCTGGACTTTATCGACAAGAGATCATGGTTGGGTTGTGGCTGACTGCTCTGCTGAAGCGTTAAAGGCTGCCCTAGAATTGTCAGAACTGCCTGAAAACATTGTTGGAAAACCACTACCCCAGCAAAGGATTTTCGATTGTGTCAATTATCTACTTTCCATGCAGAATACAAATGGTGGTTATGCAACTTATGAATTGACACGATCCTATAATTGGCTAGGGAATTTCAATCCTGCAGAGATACTTGGAGAATTTTGCATTGACCATTCGTATGTGGAATGCACGTCATCAGCAATTCAAGCTCTTGCACTTTTTAAGAAACTGCATCCACAGCACCGACGTAAAGAAATAGAACAATGCATTTCAAATGGCAGTCGGTACATTGAAAATATGCAAAGGCCTGACGGTTCATGGTATGGTTCGTGGGGAGTCTGCTTCACATATGGTACATGGTTTGGTATTCTGGGTTTAAAGGCTGCTGGCAAAACCTACCAAAACTGTTCAACGATTCGTAAAGCTTGTGATTTCCTTCTTTCAAAGCAGCTTCCTTCTGGTGGTTGGGGAGAGAGTTATATTGCATGCCAAAAGCAGGTTTATACAAACTTGGGTGATGGAAGATCACACATAGTAAACACAGCGTGGGCTATGCTAGCTCTTATTTGCGCTGGGCAGGCTTTGAGAGATCCAAAACCTCTTCATCGAGCAGCAATTGTACTTGTGAATTCACAAATGGAGAATGGTGATTTTCCACAATGGGAACTTAGGGGTGTAGGTTGCGGGTGCTTTGTGATGGGTTATGCTGCCTATCATAATATCTTCCCCATATGGGCTCTAGCTGAATATCGTCGTCATGTTCTACAATCATAA___TGTATATGTTTGTCATATCTGTCATATTTATTAAAGGTAAGTTTTTGGCCATAAACTGAATTTTGAGGGAGGTTAGAATGATCTCTAAGCATCTGCAATAAGATGGTTTATTAGTTTGTAATATATAATGATTGCAATACAATCTTGCATTTGTAATAATCCAGTAAAATAGCGTTTGCTATGCAATCATTGCGTGAATGAAGTACATGTTGATGTCTCTCTGAAATAGTAATTAACTTTCTATGGCTTAGATTATCATTGAAACGGTTTTATCAATAACCATGCTTGTGGTAGTT

Fln Alignment:

Contig9253___PQPLIQDIMWTTLHKVVEPMLGHWPGSMLRERALQTVMKHAHYDDESTRYIGMNPDSKVMNMLCCWIEDKNSEAFKKHLPRILDYLWIAEDGMKTQCHNGSQLWDAAFAIQALISADLLDECGPLLKKAHAFIEKSQVQENSPGEFSTWYRHMSKGAWTLSTRDHGWVVADCSXXXXXXXXXXXXXXENIVGKPLPQQRIFDCVNYLLSMQNTNGGYATYELTRSYNWLGNFNPAEILGEFCIDHSYVECTSSAIQALALFKKLHPQHRRKEIEQCISNGSRYIENMQRPDGSWYGSWGVCFTYGTWFGILGLKAAGKTYQNCSTIRKACDFLLSKQLPSGGWGESYIACQKQVYTNLGDGRSHIVNTAWAMLALICAGQALRDPKPLHRAAIVLVNSQMENGDFPQWELRGVGCGCFVMGYAAYHNIFPIWALAEYRRHVLQS
Q9FQM1______________PHPFLQDILWGTLHKVVEPALMHWPGSMLRERALQSVMKHIHYEDENTRYICIGPVNKVLNMLCCWVEDSNSEAFKRHLARVVDYLWVAEDGMKMQGYNGSQLWDTAFATQALISTNLLDDCGPLLKKAHIYIERSQVQEDCPGDLNFWYRHISNGAWPFSTRDHGWPISDCSSEGLKAALALSQLPQDIVGKPIPSQRIFDCVNLMLSMQNSDGGFATYELTRSYPWLEKINPAETFGDIVIDYSYVECTSAITQALVSFKKLYPEHRHKEIETCILKATRYIENIQRPDGSWYGSWGVCFTYGTWFGVLGLAAAGKTYQNCSNIRKACEFLLSKQLPSGGWGESYLSCQEKVYTHLEGGRSHIVSTAWAMLALIYAGQALRDPKPLHRAAIVLVNYQMENGDFPQQEIMGVFNRNCMISYSAYRNIFPIWALGEYCRHVLQS

SNPs (tot: 1)

Position	A %	C %	G %	T %	Probability
1038	83		16		0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain