SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene8449

Length: 1549 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene8449

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Unassigned protein	-	-	-
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	53%
Blast2go	uncharacterized protein	-	-	79%

Source	GOs	Term	Type	e value	Identity
Blast2go	vacuolar membrane	GO:0005774	Cellular Component	0.0	79%

Source	InterPros	Term	Type	e value	Identity
Sma3	Domain of unknown function DUF641, plant	IPR006943	-	0.0	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	0.0	-

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: D5AC56

Fln msg: query STOP codon is far from subject stop. Distance to subject end: 16 aas, your sequence is shorter than subject: 130 - 214

Fln protein: VVTLAVTHLQVVHPKMEESFFGNLDQRDHVVNGIHPKTDFYEAFLKLAKAIWLVHRLAFSFDPVVSIFQVRRGTDFSVVYMESVVHGVLSGYDALDGRPKVGFTVMPGFRVSKAVIQCQVYLTGIKGRD*

Protein Length: 131

Fln nts: CGTCGTGACCTTGGCTGTCACTCATTTGCAGGTTGTGCATCCCAAGATGGAGGAATCCTTTTTTGGGAATCTTGACCAAAGGGACCATGTTGTCAATGGCATACATCCGAAAACGGACTTCTATGAAGCCTTCTTGAAGCTTGCCAAGGCAATCTGGCTTGTGCACAGATTGGCATTTTCCTTTGACCCTGTTGTCAGTATATTTCAGGTTAGGAGGGGGACTGACTTTTCAGTGGTGTATATGGAGAGTGTTGTACATGGAGTGTTGTCTGGTTACGATGCTTTGGATGGGAGGCCTAAGGTGGGTTTTACTGTTATGCCGGGTTTCCGTGTCAGCAAGGCCGTGATCCAGTGCCAAGTTTATCTCACAGGCATAAAAGGCAGGGATTAG___TTGGAAGGATTGGAAGGACTCTGTGTCAATCGCATGAATTAGCCTTGTTATCTAAACCCATTTGTTTGAATTTAGGTCAAAACAGAGACTGATTTACACACAGATCAGAATGCATAGAGAAGCCAGTTAGGTATTCGTTGTAGTTTTTGAGAATTTCTCATAAACTGCCCATGTGGAAAGCCTTAATATTTCAGAACATTATCTGGAAGTAAAGCCAACCTCATAATGAATGCTAATATATTTTAGGCAACAGAGTTCCTGTGGTTTTCTTCATGATCTGGTTTTCGGCATGTATAATGTTAATGCGAGGGCTTTGAGCGGAGCATAGATTCAAATTTGACAGTCGGGCCCAGTCATCAATGCGCAGAGCTTCTTTGAATATGTAGGGCAGTTCCGGACAATGTTTAGCTTCAAAAGTTGCCAAGGCAACAGTTACAGTATATGAAGCTGTGCAGAATGTGTTCAAATTGTGTCCGTGACTTGAGGTGTAGAAATGAGCACTGATCTTGTTGGATCAGGAAAAATAATCTGCGATGGCTATTGACATCAGACCCTTTTTATCTATGTTAGGAGGCCACCCTTGGTGGAAATGTATCGTTTAAAATAATTAGATTATGTGCAGAAGGCAGTATAATTCATGACTCTCAAGATGGTGCTACAGTAAGTGAGTCTTCTGCCTGTAAAGTTGAGGGCTTTCAACATTGTTTGAGATGGATACTTATGCAGAGGAAGCTGAATTTTTTTCAAAAGTACTTTTTCTGGGATTTTGAGCTAACAAAGCTCAGGGCTTAAACATAACCCTATGCTTATTAGACACTTACTTTCTCGCCATGGCTTCGGAGGTCCACAGCTGATCGGATTGTATACTTTCTATCAAAATTCTCAGCTGCCCACTAACTCATGTGTTGTACACCTTTGTTTCACCTAAAAAGTGAGGAGATCACCAAGGTTGTATCATCCAAACGAATGCAGCATGATCAAGCCCCTACTTCAATTAGTGTTCTCAGGAATGGTTTACTTATGTGCGTACTCAGTACTTATATTTATTATTATTTCTTTTAGTTTTGATGTAGCTAAGTGAAATTTGAAAATACAAGTGCAAGGGATGTCTTGGGTTATTGCGTGTTTTTAATACAAATCATACAGTAGATTGCTTAT

Fln Alignment:

Contig8449___LQVVHPKMEESFFGNLDQRDHVVNGIHPKTDFYEAFLKLAKAIWLVHRLAFSFDPVVSIFQVRRGTDFSVVYMESVVHGVLSGYD-----ALDGRPK----------VGFTVMPGFRV-SKAVIQCQVYL
D5AC56______________LHVVHPRMEESFFGNLEHRDQIAKGLHPKSQFYSLFLKFAKSVWLVHRLAFSFAPPVSIFHVKRGVDFHDSYMDS-LNSVNMNHDVANNVATEGNTQEHPEKQNPTIIGFTVMPGFRVGKKAIVKCQVYV

SNPs (tot: 12)

Position	A %	C %	G %	T %	Probability
559		75		25	0.996473
660	10	90			0.90298
661	10			90	0.90298
663		90	10		0.90298
664	90			10	0.90298
740		60		40	1.0
864			11	88	0.923564
1132	33			66	0.997452
1133		33		66	0.997452
1167		80		20	0.995493
1330			88	11	0.923564
1350		85		14	0.993533

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain