SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene8406

Length: 2582 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene8406
CGTCGTGACCTTGGCTGTCACTCAGTCAATGGGTGTCAAACAATAAAACTAATAAATTGCAAATCCCAGCTGCTACGAGACCAGAGAATAGCATGAGGTTAGATAGTTTTCCGTCGCTTTAATCATCACATTAGAGAAATAGAGTAGCAGCTTTGCAGCGGCGACACAGACGCCAAACGCAGTTCAGGGGAGCTGCTTCTTTTGTCAAACATAAAGCAAGATGGGGAGCACAGGTGGAAGTGGAAGTATTGGTGGAGTAACAAATGTTCGTGTAGCGGTGATAGGAGACAGTGGTACTGGGAAGTCTAGTTTGATTGTTTCTGTAGCCACTGATTCGTTTCCAGAGAGGGCTCCTCCACTTTTGCCCCCAACAAGACTCCCTCCAGATTTCTATCCTGATCGTGTTCCACTCACCATAATTGATACATCATCCAGACCCGAGGACCGTAACAAGCTAGGAGCAGAATGCAAGAAGGCAGATGCAATTGTTCTAACATATGCATGTGAACAACCTATCACACTTGAGCGTCTGTCTTCCTACTGGCTACCAGAGCTTCGTCGCTTAGAAGTGAAAGTGCCAGTCATTGTGGTTGGTTGTAAATTAGATCTGCGAGATGATCAACAACTAAGTTTGGAGCAAGTAATGACACCAATCATGCATCAGTTTCGAGAGATTGAGACATGTATAGAGTGTTCAGCACTGAAGCAGATTCAGGTTGCAGAAGTTTTTTACTATGCTCAAAAAGCAGTTCTTCATCCAACAGCACCTTTATTTGATCAAGAAACCCAAACACTAAAGCCTCGTTGTGTAAGGGCATTGAAAAGAATCTTCATTCTATGCGATCATGACAGAGATGGAGCTCTTAGTGATGCTGAACTGAATGATTTTCAGGTTAAATGCTTTAATGCTCCTCTACAGCCTTCGGAAATTGTTGGGGTGAAGAAGGTTGTTTCGGAAAAACTTCCAGAAGGCGTCAATGAACATGGCCTCACATTGACAGGATTTCTTTTTCTCCATGCACTTTTTATTGAAAAAGGACGGCTTGAAACCACCTGGACAGTTTTGCGAAAGTTTGGTTATAATGATGAGATTAAGCTTAGAGATGACCTCCTTCCAACAGCACAGTTCAAACGGGCACCCGATCAGAGCATAGAGTTAACCAGTGAAGCAGTGGATTTCCTAAGGGGTATCTTTTCGTTGTTTGACATTGATGGTGATGGGGCACTGCGACCACAAGAGCTTGAAGATCTTTTTTCCACAGCTCCAGAAAGTCCATGGGAAGAAGGTATCTACGAGGATTCAATGGAGAAGAATGCATTGGGAGCTGTGACCATAAATGGATTTCTATCTGCGTGGGCTCTCATGACTCTTATGGATCCTGTCAAAAGCCTTGCTTATTTAATTTATATTGGGTACCCCGGAGATCCTGTTTCAGTATTTCATGTGACCCGTCGTCGACGGCTTGACCGCAAAAGATTAAATTCGCAGAGGAACGTCTTTCAGTGTTTTGTTTTTGGACCTGGTAATGCTGGGAAGTCTGCATTACTCAATGCTCTAATTGGCAGGCCATTCGTTGAAAAATACACACCAACAAAAGGTGATTGTTATGCTACAAATGTTATTGAAATGAGCGAGGGGGCAAAGAAAATGCTAGTCATGCGTGAAATTCTGGAAGGTGCAGTGCACAATCTTTTGGCAAATAAAGAATTCTTGGCTCCATGCGATGTGGCCGTATTTGTTTATGATAGTTCAGATGAGGCTTCATGGAGAAAAACTGCTGAACTGCTAGTGGAAGTTGCCACACATGCAGAAAATAGTGGCTTTGAGGTTCCATGTGTTATGGTTGCTGCAAAAGATGATTTAGATCCACATCCTGTGTCGATAACAGATTCAGCAAGGGTTAGCCAAGATATGGGTGTGGAATCCCCGATTACTGTCAGCATGAAATTGGGAGACATAAGTAACCTATTTCGTCGGATCATCAATGTTGCCCAACGTCCACATCTAAGCATCCCGGAGACCGAGGCTGGTAAAAACCACAAGCAATATCGACGGCTAGTCAATCGGTCACTCATGTTTGTGTCTGTTGGGGCAGCCGTTACAGTTGCGGGCATAGCAGCTTACAGGATGTATGCAGTAAGGAAGCACAGCTCTAGTTAACTACTGCAGTCTCATTTAAAGTCTTTTTTGTAAGCTTTCAGCTGGTTGATATGTATTCTGTCAAAGTGGTCTTTGCTAGTCAGGACCAAAATTGTAAGTATACCTCTCGAGAGGTATATGGTCAGGGCATTTAGAGTCCTTGTGCACCTGCTGCCGTAGGTTGTAGTCCTGTCTGTTATTTCACATATATTTTATGCAGTCCTTGTCAGGTGCCCTCGAGTGTGGATGTATGTGTACATCAGTATGATGTTGAAATGAGTTGTTTGTTCAATAAAATTTGATTTTGTTGAAGCTCCCACCACTTTTTAGTTCTAGAATATTGTGTATGTTTGCTATTCCATTTAGATGTCCTGTGAAATTTGGTCATAGAAGTTACTCTGCAATCTATACTGTTACTGATGCAATAAACCAATTTATCTAG

Ace file of the UniGene sp_v2.0_unigene8406 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Mitochondrial Rho GTPase n=2 Tax=Selaginella moellendorffii RepID=D8RQC7_SELML	-	-	67%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	93%
Blast2go	protein	-	-	83%

Source	GOs	Term	Type	Identity
Blast2go	mitochondrion transport along microtubule	GO:0047497	Biological Process	83%
Blast2go	small GTPase mediated signal transduction	GO:0007264	Biological Process	83%
Blast2go	pollen tube growth	GO:0009860	Biological Process	83%
Blast2go	mitochondrion organization	GO:0007005	Biological Process	83%
Blast2go	embryo development ending in seed dormancy	GO:0009793	Biological Process	83%
Blast2go	calcium ion binding	GO:0005509	Molecular Function	83%
Blast2go	hydrolase activity	GO:0016787	Molecular Function	83%
Blast2go	GTP binding	GO:0005525	Molecular Function	83%
Blast2go	mitochondrial outer membrane	GO:0005741	Cellular Component	83%

Source	InterPros	Term	Type	Identity
Sma3	Small GTPase superfamily	IPR001806	-	-
Sma3	Calcium-binding EF-hand	IPR002048	-	-
Sma3	Small GTP-binding protein domain	IPR005225	-	-
Sma3	EF-hand-like domain	IPR011992	-	-
Sma3	EF hand associated, type-1	IPR013566	-	-
Sma3	EF hand associated, type-2	IPR013567	-	-
Sma3	Mitochondrial Rho-like	IPR013684	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-
Sma3	EF-hand	IPR018248	-	-
Sma3	EF-HAND 2	IPR018249	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5ADJ6

Fln msg: your sequence is longer than subject: 647 - 177

Fln protein: MGSTGGSGSIGGVTNVRVAVIGDSGTGKSSLIVSVATDSFPERAPPLLPPTRLPPDFYPDRVPLTIIDTSSRPEDRNKLGAECKKADAIVLTYACEQPITLERLSSYWLPELRRLEVKVPVIVVGCKLDLRDDQQLSLEQVMTPIMHQFREIETCIECSALKQIQVAEVFYYAQKAVLHPTAPLFDQETQTLKPRCVRALKRIFILCDHDRDGALSDAELNDFQVKCFNAPLQPSEIVGVKKVVSEKLPEGVNEHGLTLTGFLFLHALFIEKGRLETTWTVLRKFGYNDEIKLRDDLLPTAQFKRAPDQSIELTSEAVDFLRGIFSLFDIDGDGALRPQELEDLFSTAPESPWEEGIYEDSMEKNALGAVTINGFLSAWALMTLMDPVKSLAYLIYIGYPGDPVSVFHVTRRRRLDRKRLNSQRNVFQCFVFGPGNAGKSALLNALIGRPFVEKYTPTKGDCYATNVIEMSEGAKKMLVMREILEGAVHNLLANKEFLAPCDVAVFVYDSSDEASWRKTAELLVEVATHAENSGFEVPCVMVAAKDDLDPHPVSITDSARVSQDMGVESPITVSMKLGDISNLFRRIINVAQRPHLSIPETEAGKNHKQYRRLVNRSLMFVSVGAAVTVAGIAAYRMYAVRKHSSS*

Protein Length: 648

Fln nts: CGTCGTGACCTTGGCTGTCACTCAGTCAATGGGTGTCAAACAATAAAACTAATAAATTGCAAATCCCAGCTGCTACGAGACCAGAGAATAGCATGAGGTTAGATAGTTTTCCGTCGCTTTAATCATCACATTAGAGAAATAGAGTAGCAGCTTTGCAGCGGCGACACAGACGCCAAACGCAGTTCAGGGGAGCTGCTTCTTTTGTCAAACATAAAGCAAG_-_ATGGGGAGCACAGGTGGAAGTGGAAGTATTGGTGGAGTAACAAATGTTCGTGTAGCGGTGATAGGAGACAGTGGTACTGGGAAGTCTAGTTTGATTGTTTCTGTAGCCACTGATTCGTTTCCAGAGAGGGCTCCTCCACTTTTGCCCCCAACAAGACTCCCTCCAGATTTCTATCCTGATCGTGTTCCACTCACCATAATTGATACATCATCCAGACCCGAGGACCGTAACAAGCTAGGAGCAGAATGCAAGAAGGCAGATGCAATTGTTCTAACATATGCATGTGAACAACCTATCACACTTGAGCGTCTGTCTTCCTACTGGCTACCAGAGCTTCGTCGCTTAGAAGTGAAAGTGCCAGTCATTGTGGTTGGTTGTAAATTAGATCTGCGAGATGATCAACAACTAAGTTTGGAGCAAGTAATGACACCAATCATGCATCAGTTTCGAGAGATTGAGACATGTATAGAGTGTTCAGCACTGAAGCAGATTCAGGTTGCAGAAGTTTTTTACTATGCTCAAAAAGCAGTTCTTCATCCAACAGCACCTTTATTTGATCAAGAAACCCAAACACTAAAGCCTCGTTGTGTAAGGGCATTGAAAAGAATCTTCATTCTATGCGATCATGACAGAGATGGAGCTCTTAGTGATGCTGAACTGAATGATTTTCAGGTTAAATGCTTTAATGCTCCTCTACAGCCTTCGGAAATTGTTGGGGTGAAGAAGGTTGTTTCGGAAAAACTTCCAGAAGGCGTCAATGAACATGGCCTCACATTGACAGGATTTCTTTTTCTCCATGCACTTTTTATTGAAAAAGGACGGCTTGAAACCACCTGGACAGTTTTGCGAAAGTTTGGTTATAATGATGAGATTAAGCTTAGAGATGACCTCCTTCCAACAGCACAGTTCAAACGGGCACCCGATCAGAGCATAGAGTTAACCAGTGAAGCAGTGGATTTCCTAAGGGGTATCTTTTCGTTGTTTGACATTGATGGTGATGGGGCACTGCGACCACAAGAGCTTGAAGATCTTTTTTCCACAGCTCCAGAAAGTCCATGGGAAGAAGGTATCTACGAGGATTCAATGGAGAAGAATGCATTGGGAGCTGTGACCATAAATGGATTTCTATCTGCGTGGGCTCTCATGACTCTTATGGATCCTGTCAAAAGCCTTGCTTATTTAATTTATATTGGGTACCCCGGAGATCCTGTTTCAGTATTTCATGTGACCCGTCGTCGACGGCTTGACCGCAAAAGATTAAATTCGCAGAGGAACGTCTTTCAGTGTTTTGTTTTTGGACCTGGTAATGCTGGGAAGTCTGCATTACTCAATGCTCTAATTGGCAGGCCATTCGTTGAAAAATACACACCAACAAAAGGTGATTGTTATGCTACAAATGTTATTGAAATGAGCGAGGGGGCAAAGAAAATGCTAGTCATGCGTGAAATTCTGGAAGGTGCAGTGCACAATCTTTTGGCAAATAAAGAATTCTTGGCTCCATGCGATGTGGCCGTATTTGTTTATGATAGTTCAGATGAGGCTTCATGGAGAAAAACTGCTGAACTGCTAGTGGAAGTTGCCACACATGCAGAAAATAGTGGCTTTGAGGTTCCATGTGTTATGGTTGCTGCAAAAGATGATTTAGATCCACATCCTGTGTCGATAACAGATTCAGCAAGGGTTAGCCAAGATATGGGTGTGGAATCCCCGATTACTGTCAGCATGAAATTGGGAGACATAAGTAACCTATTTCGTCGGATCATCAATGTTGCCCAACGTCCACATCTAAGCATCCCGGAGACCGAGGCTGGTAAAAACCACAAGCAATATCGACGGCTAGTCAATCGGTCACTCATGTTTGTGTCTGTTGGGGCAGCCGTTACAGTTGCGGGCATAGCAGCTTACAGGATGTATGCAGTAAGGAAGCACAGCTCTAGTTAA___CTACTGCAGTCTCATTTAAAGTCTTTTTTGTAAGCTTTCAGCTGGTTGATATGTATTCTGTCAAAGTGGTCTTTGCTAGTCAGGACCAAAATTGTAAGTATACCTCTCGAGAGGTATATGGTCAGGGCATTTAGAGTCCTTGTGCACCTGCTGCCGTAGGTTGTAGTCCTGTCTGTTATTTCACATATATTTTATGCAGTCCTTGTCAGGTGCCCTCGAGTGTGGATGTATGTGTACATCAGTATGATGTTGAAATGAGTTGTTTGTTCAATAAAATTTGATTTTGTTGAAGCTCCCACCACTTTTTAGTTCTAGAATATTGTGTATGTTTGCTATTCCATTTAGATGTCCTGTGAAATTTGGTCATAGAAGTTACTCTGCAATCTATACTGTTACTGATGCAATAAACCAATTTATCTAG

Fln Alignment:

Contig8406___MSEGAKKMLVMREILEGAVHNLLANKEFLAPCDVAVFVYDSSDEASWRKTAELLVEVATHAENSGFEVPCVMVAAKDDLDPHPVSITDSARVSQDMGVESPITVSMKLGDISNLFRRIINVAQRPHLSIPETEAGKNHKQYRRLVNRSLMFXXXXXXXXXXXXXAYRMYAVRKH
D5ADJ6______________MNEGTKKILVMREILEDGVHKLLANKEFLAPCDVAVFVYDSSDEASWRKTAELLVEVATHAENSGFEVPCVMVAAKDDLDPHPVSISDSARVSQDMGVESPITVSMKLGDTSNLFRRIINVAQHPHLSIPETEAGKNHKQYRRLINRSLMFVSVGAAVTVVGIAAYRVYAVRKH

SNPs (tot: 53)

Position	A %	C %	G %	T %	Probability
1463	9			90	0.999872
1468	9		90		0.999872
1469	90	9			0.999872
1472		9	90		0.999872
1479		9		90	0.999872
1480		90	9		0.999872
1481	9			90	0.999872
1483			9	90	0.999872
1498		88		11	0.999937
1543			90	10	0.90298
1549		16		83	0.994512
1686	16			83	0.994512
1687		16		83	0.994512
1689	83			16	0.994512
1690		83		16	0.994512
2200	10		90		0.90298
2203			77	11	0.968132
2208		88	11		0.923564
2209	11			88	0.923564
2210		11		88	0.923564
2212		12		75	0.975346
2213		12		87	0.992554
2214	12		62	12	0.962769
2215	12		87		0.992554
2218			87	12	0.992554
2221	87		12		0.992554
2223	87	12			0.992554
2387			83	16	0.999991
2388		83	16		0.999991
2391		80		20	0.999996
2392			16	83	0.999991
2393	15	84			0.999986
2395	15	84			0.999986
2396		84	15		0.999986
2397		15		84	0.999986
2398		15	84		0.999986
2401		84	15		0.999986
2402			15	84	0.999986
2403			84	15	0.999986
2408	15			84	0.999986
2409		13	86		0.999973
2494	90	10			0.90298
2495		11		88	0.923564
2496		11		88	0.923564
2509	87	12			0.992554
2510		12	87		0.992554
2513			87	12	0.992554
2560		14	85		0.993533
2561	85	14			0.993533
2562		14	85		0.993533
2563		14		85	0.993533
2564		14	85		0.993533
2566		85		14	0.993533

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain