SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene8298

Length: 915 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene8298

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Amidase, putative [Oryza sativa Japonica Group]	-	-	55%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	26%
Blast2go	glutamyl-trna amidotransferase subunit a-like	-	-	76%

Source	ECs	Term	Type	e value	Identity
Blast2go	Ligases, Forming carbon-nitrogen bonds, Carbon--nitrogen ligases with glutamine as amido-N-donor.	EC:6.3.5.-	-	0.0	76%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Tetracycline biosynthesis	00253		0.0	76%
Blast2go	Biosynthesis of secondary metabolites	01110		0.0	76%

Source	GOs	Term	Type	e value	Identity
Blast2go	carbon-nitrogen ligase activity, with glutamine as amido-N-donor	GO:0016884	Molecular Function	0.0	76%

Source	InterPros	Term	Type	e value	Identity
Sma3	Amidase	IPR000120	-	0.0	-

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NVZ3

Fln msg: STOP codon was not found. Distance to subject end: 8 aas, your sequence is shorter than subject: 304 - 538

Fln protein: AVSTGLCPAALGVDGGGSVRMPAAMCGVVGLKPTYGRIPHLGVLPFNWTVGMVGVLAATVEDALIVYAAILGHLPTDRLVSFPPHTRLPLLENDKSVGNLKLAKYTKWFEHCDDDIRNTCQWALNLLQCHYGCKTLEVTLPELEEMRLAHYITIGSECDASLGVDFAKLGMARSGGDTRIALHVYKNFSSREFLNAQRLRSRQMYIHMEIFKKANVIVTPTTGITAYRLQEDALSYGEFDYANGAAMLRYQVAGNFLGLPAVTVPIGYDKFGMPIGLQFIGRPWSEATLLHLAYAIEVLCIKER

Protein Length: 305

Fln nts: CAGCTGTATCTACGGGATTGTGCCCTGCTGCACTTGGTGTTGATGGTGGAGGATCTGTGCGAATGCCAGCAGCAATGTGTGGTGTAGTTGGTCTTAAGCCCACTTATGGACGCATACCCCACTTAGGTGTGCTTCCATTCAACTGGACGGTTGGAATGGTTGGTGTGCTGGCAGCAACGGTGGAGGATGCGCTGATTGTATATGCAGCCATTTTGGGACATCTTCCAACCGATCGATTGGTTTCATTTCCCCCTCATACAAGGCTACCGTTGCTGGAAAACGACAAGAGCGTTGGTAATCTCAAGCTTGCCAAATATACCAAGTGGTTCGAGCATTGTGATGACGATATAAGGAATACTTGTCAGTGGGCATTAAACTTATTACAATGCCATTATGGATGTAAGACACTTGAGGTGACTTTGCCAGAGTTGGAGGAAATGCGCCTTGCACATTATATTACGATTGGTTCTGAATGTGATGCTTCTTTGGGTGTTGATTTTGCAAAACTAGGCATGGCAAGATCAGGTGGAGATACGAGGATTGCATTGCATGTTTATAAAAACTTTAGCAGCCGAGAGTTCCTAAATGCACAACGACTAAGGTCTCGTCAAATGTACATTCATATGGAAATATTCAAAAAGGCAAATGTGATTGTCACACCCACAACCGGAATAACTGCATATCGCCTTCAAGAAGATGCACTTAGCTATGGGGAGTTTGATTATGCCAATGGTGCTGCAATGTTGCGGTATCAAGTAGCTGGAAATTTTCTAGGACTTCCAGCAGTGACTGTTCCGATTGGATACGACAAGTTTGGAATGCCTATTGGTCTTCAATTCATTGGTCGTCCCTGGTCTGAGGCAACATTGCTTCACCTAGCTTATGCAATAGAGGTTTTATGCATTAAAGAAAGGA

Fln Alignment:

Contig8298___AVSTGLCPAALGVDGGGSVRMPAAMCGVVGLKPTYGRIPHLGVLPFNWTVGMVGVLAATVEDALIVYAAILGHLPTDRLVSFPPHTRLPLLE---------NDKSVGNLKLAKYTKWF-EHCDDDIRNTCQWALNLLQCHYGCKTLEVTLPELEEMRLAHYITIGSECDASL----GVDF----------AKLGMARSGG-----DTRIALHVY---KNFSSREFLNAQRLRSRQMYIHMEIFKKANVIVTPTTGITAYRLQE---DALSYGEFDYANGAAMLRYQVAGNFLGLPAVTVPIGYDKFG---MPIGLQFIGRPWSEATLLHLAYAIE
A9NVZ3______________AVAARQCAVSLGSDTGGSIRQPASFCGVVGMKPTYGRVSRFGLMAYASSLDVIGCLGSSVMDAAILLGAMAGH---DKLDATSSKTEVPDYAAKLVPIEALDSKPLKGIKIGVIRETMGDGVDSGVISSIQASALRLE-ELGAALTEVSLSSFPLGLPAYYVLAPSEASSNLARYDGIRYGSQVPADDLESMYGSSRSNGFGQEVKRRILMGTYALSAGYYDAYYKRAQQVRTLIQRDFSKALDGNDILISPAAPTAAYKIGDKVNDPLSMYAGDIMT--------VNVNLAGLPALVVPCGFVKVGERDLPVGLQMISAAFEEGRLLQVGHIFQ

SNPs (tot: 11)

Position	A %	C %	G %	T %	Probability
471	25			75	1.0
496		14		85	0.993533
517			14	85	0.993533
850	16		83		0.994512
851			16	83	0.994512
852	16	83			0.994512
855	16			83	0.994512
858			20	80	0.995493
859	25	75			0.996473
860		25		75	0.996473
863		25	75		0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain