SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene8172

Length: 1542 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene8172 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	pectate lyase [Fragaria x ananassa]	-	-	72%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	83%
Blast2go	pectate lyase	-	-	88%

Source	ECs	Term	Type	e value	Identity
Blast2go	Pectate lyase.	EC:4.2.2.2	-	0.0	88%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Pentose and glucuronate interconversions	00040		0.0	88%

Source	GOs	Term	Type	e value	Identity
Blast2go	pectate lyase activity	GO:0030570	Molecular Function	0.0	88%

Source	InterPros	Term	Type	Identity
Sma3	Domain of unknown function DUF11	IPR001434	-	-
Sma3	Pectate lyase/Amb allergen	IPR002022	-	-
Sma3	Parallel beta-helix repeat	IPR006626	-	-
Sma3	Pectate lyase, N-terminal	IPR007524	-	-
Sma3	Pectin lyase fold	IPR012334	-	-
Sma3	AmbAllergen	IPR018082	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LQ36

Fln msg: your sequence is shorter than subject: 361 - 426

Fln protein: QLLFALSTETGSGPKFVTSEFWVFARMCNRVVLCGIGFGRNAIGGKNGRFYRVTDPNDYDAVNPRPGTLRHAVIQTEPLWIVFERDMVITLKEELIMNSFKTIDGRGVNVHIANGPCITVQYVTNIIIHGIHVHDCKPAGNAMVRDSPTHYGWRTMCDGDGISIFGGSHIWVDHVSLSNCADGLIDAIMGSTAITVSNSYFTHHNEVMLLGHSDSYTPDVNMQVTIAFNHFGEGLRQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFSAPADPFAKEVTKREDNNENEWRQWNWRSEGDMLLNGAFFVSSGAGAASAYAKASSLGARPSSLVQPLTATAGVLTCRRGVRC*

Protein Length: 362

Fln nts: GCAATTACTGTTTGCTTTGAGTACAGAGACCGGATCTGGTCCTAAATTTGTAACTAGTGAGTTTTGGGTGTTTGCCCGTATGTGCAATCGTGTTGTCTTGTGCGGCATTGGCTTCGGCAGGAATGCTATTGGAGGCAAGAATGGGAGGTTTTACCGGGTCACGGATCCCAACGATTACGATGCTGTGAACCCGAGACCGGGCACTCTGCGTCATGCCGTGATTCAAACAGAGCCTCTCTGGATCGTCTTCGAGAGAGACATGGTGATCACACTGAAGGAGGAGCTGATCATGAACAGTTTCAAGACAATTGACGGAAGGGGCGTCAATGTCCACATTGCAAACGGGCCCTGCATCACTGTTCAGTATGTGACTAACATCATTATACATGGCATTCACGTTCATGATTGTAAGCCTGCAGGAAATGCTATGGTAAGGGACTCTCCCACGCATTATGGGTGGAGAACTATGTGCGATGGAGATGGAATCTCCATCTTTGGAGGAAGCCACATTTGGGTGGACCATGTCTCCTTGTCCAACTGTGCTGACGGTCTCATTGACGCCATCATGGGATCCACTGCTATTACCGTTTCAAACAGCTATTTCACTCACCACAATGAGGTGATGTTGTTGGGTCACAGTGATAGCTATACTCCCGATGTCAACATGCAGGTCACGATTGCATTTAATCATTTTGGGGAAGGTCTTAGGCAGAGAATGCCCAGGTGCAGGCATGGATACTTCCATGTGGTAAACAATGACTACACTCACTGGGAAATGTACGCAATTGGGGGCAGTGCAAACCCCACAATCAACAGTCAAGGCAATAGATTCTCGGCACCTGCAGACCCCTTTGCCAAAGAGGTAACAAAGAGGGAAGACAACAATGAGAATGAATGGAGACAGTGGAACTGGAGATCGGAAGGAGATATGCTGCTGAACGGTGCATTTTTTGTTTCATCGGGAGCAGGGGCGGCCTCGGCGTATGCAAAAGCATCGAGTCTGGGAGCCAGGCCTTCCAGCCTCGTCCAACCGCTTACCGCGACTGCAGGTGTACTCACTTGCAGGAGAGGAGTTCGCTGCTAA___CACCCTCTACTACTAACCCATTTGCTGACCCACCAAGTTCAAGTTCAAGTTCAACCAACCAGTGAATGTGAAGTAATCATTATTTTGGCAATTAAGCAACTCCCATTAACGCCCATATCTGGTGTTTTGGGGCCTTTTCAAAATTTCCCATCATTGTGTATTGTATTGTTTGGAGAGCTATAAGTGCCTCCACATTTTCGTTAGCAACCTCGGCCTGCACTCTGTTGCAATCATTCAACTGACAGGCTTTCCAACTTGGAATGCAGTTGTTTTCTAACAGCAAGAGCAGGCGCAGAAGAGTTGTTTTCGCTCTTGTTTTTAATTTTTGTTGTTGTGGTGGGTACCATGTCATCCGCAATTGTGTAATTATGCTCTGCTCAAAGCAAAGTCCTTCTATTTTCTCACTATTTGCCTTTTCCTGTGTGTTTGCTCCTTTGCCTACAATTCTCGAAGACATT

Fln Alignment:

Contig8172___RVVLCGIGFGRNAIGGKNGRFYRVTDPNDYDAVNPRPGTLRHAVIQTEPLWIVFERDMVITLKEELIMNSFKTIDGRGVNVHIANGPCITVQYVTNIIIHGIHVHDCKPAGNAMVRDSPTHYGWRTMCDGDGISIFGGSHIWVDHVSLSNCADGLIDAIMGSTAITVSNSYFTHHNEVMLLGHSDSYTPDVNMQVTIAFNHFGEGLRQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFSAPADPFAKEVTKXXXXXXXXXXXXXXRSEGDMLLNXXXXXXXXXXXXXXXXXXXXXXARPSSLVQPLTATAGVLTCRRGVRC
B8LQ36______________RLADCAIGFGRDALGGKHGSFYLVTDPNDYDAVNPRPGTLRHAVIQTEPLWIIFKRDMVIQLKEELIMNSYKTIDGRGVNVHIANGPCITVQYVSHIIIHGIHVHDCKPQGNAMVRDSPTHYEWRTS-DGDGISIFGGSHVWVDHVSLSNCADGLIDATMGSTAITISNSYFTHHNEVMLLGHSDSHTSDVKMRVTVAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFLAPANPLAKEVTKRINQDVGEWKEWNWRSNGDMMLNGAYFTPSGAGESSAYIRASSLPARPSSLVGPLTQNAGVLFCRKGGRC

SNPs (tot: 68)

Position	A %	C %	G %	T %	Probability
81	37		62		1.0
125	87			12	0.992554
146	42			57	1.0
221	10		90		0.90298
271	88			11	0.923564
273	11		88		0.923564
276		88	11		0.923564
277		11		88	0.923564
278		11		88	0.923564
283		88	11		0.923564
286	11			88	0.923564
288		88	11		0.923564
290	88	11			0.923564
291	88		11		0.923564
906	10			90	0.90298
907	10		90		0.90298
911		90		10	0.90298
930	14			85	0.993533
934		14	85		0.993533
936	14		85		0.993533
937	85	14			0.993533
939		16		83	0.994512
1271	11	88			0.923564
1272	11			88	0.923564
1273	11			88	0.923564
1276		11	88		0.923564
1278		11	88		0.923564
1279		11		88	0.923564
1280	11		88		0.923564
1427		87		12	0.992554
1429	25			75	1.0
1430	25		75		1.0
1431		75		25	1.0
1437	28	71			1.0
1443	14	85			0.993533
1444	28	71			1.0
1445		16	83		0.994512
1446		16	66		0.988813
1447	16			50	1.0
1448		83	16		0.994512
1449	50		16	16	0.988986
1450	50	33	16		1.0
1451		16	50	33	1.0
1452	50	16	16	16	0.988986
1453		33	16	50	1.0
1454	50	16	16	16	0.988986
1455	16	66	16		0.988813
1456		80	20		0.995493
1457	25	75			0.996473
1458	25	75			0.996473
1460	75			25	0.996473
1463			66	33	0.997452
1464		33		66	0.997452
1465	66			33	0.997452
1466		66	33		0.997452
1467	66			33	0.997452
1469	33	66			0.997452
1470		33		66	0.997452
1471			33	66	0.997452
1472			33	66	0.997452
1473			66	33	0.997452
1474		33		66	0.997452
1475	66	33			0.997452
1476	66	33			0.997452
1479	66			33	0.997452
1481		66	33		0.997452
1482		66	33		0.997452
1483	66		33		0.997452

SSRs (tot: 1)

Start position	End position	Sequence	Length
405	425	TTGAAC TTGAAC TTGAAC TTG	21

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain