SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene6918

Length: 2963 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene6918
ATGGGGGAACTTCTTAACTTGTGCCTCTTCCTTAATGCATTTGAAAATTTCACTATATACTTTCAAAGGATTTCCTCGCTAAGAAACTGTCCTGGAATATCAACGTTCCCTCTTGCACTTGCTCCTGCTGCGTTCCTTGAAGAATCAATCAGCTGAGTAACATGCTTCTCTAATTTTTGTTTGTTGCGCTGATCGATCCTGGGAATTTTCCTGAGTAGGTCGGTGAAGATTATTTGCAGGAGAAGCTATGGGCCTTGGAAGAGTTGATATGGATGTGGGATCTGATGGCGTCGCGCTTATAACCATCACAAACCCCCCTGTCAATTCATTGTCAGTTGAAGTCTTGCTAGGCTTGAAAGAGAAATATGATGAAGCTTTGCAAAGACATGACGTGAAAGCAATAGTTATTACAGGTTCTAGTGGCAAATTCTGTGGGGGTTTTGATATAGGAACCTTGGGGGGATTACAAGGTGCGGCTGTACAAATTGAAGGAGAACAGTTTAAATTGACAGATGTTTCAGTCAGAATCATAACCGAATTGTTTGAAGATGCAAAGAAACCGTCGGTTGCTGCTATTGACGGTCTTGCGCTTGGTGGTGGACTTGAGATTGCAATGGCTTGCCATGGACGCATAGCTGCACCACAGGCTCAACTTGGGTTGCCTGAACTCCAACTTGGAGTTATCCCTGGTTTTGGTGGTACACAGCGGCTTCCACGCCTAGTGGGCCTTTCAAAGGCACTTGAAATGATACTTTTGTCGAAACCGATCAAATCTGAAGAGGCTAGTGAGTTGGGGCTTGTGGATGCTGTAGTCCCACCTGTGGAATTGCTAAACACAGCTCGTCGCTGGGCATTGGACATTGCAGAGTTTCGAAGGCCTTGGATAAGAAGCCTTTACCGGACCGACAAGTTAGAACCTTTGGGAGAGGCACGTGAAATCCTAAAATTTGCACGAATTCAAGCACAAAAGAGAGCTGCTAATCTCAAACATCCACTTGTTTGCCTCGATGTCATTGAGGAGGGAATTGTATCTGGACCAATGGCCGGCTTGCTAAAGGAAGTAAGTTCTTTTCAGCAACTTCTTCATTCTGATACCGCCAAGTCATTAATTCATGTTTTCTTTGCACAGCGGTCGACATCAAAGGTTCCTGGAGTCACAGATGTAGGCTTAAAGCCAAGAAGAGTCAGAAAGGTAGCTGTTATTGGTGGGGGTTTGATGGGTTCTGGCATAGCCACAGCCCTAATTCTGAGTGGCTATCCTGTGATCCTTAAAGAAGTAAATGATAAATTCCTTCATTCTGGTCTTGAACGAATTAAAGCAAATTTGCAGAGCCGTGTGAATAAAGGAAAAATGAGTCAAGAAAAGTTTGAGAAAATATTGTCCCTGGTGCAGGGTACAGTTGACTACGAAAATTTTAGGAATGTGGATATGGTAATCGAAGCTGTCATTGAAAATGTAGCTCTGAAACAACAAATATTTAGTGATCTCGAGAAACACTGTCCATCACATTGTGTTCTGGCAACGAATACTTCTACACTCGACTTAAACCTGATTGGCCAGAAAACCAATTCACAGGATCGGATTGTTGGTGCACACTTTTTCAGTCCTGCACATATAATGCCTCTTCTCGAAATAGTGCGCACTGAAAAAACATCCCCCCAAGTTATAGTTGATCTCATGGATGTTGGAAAAAAAATTAAGAAGACACCTATAGTGGTTGGGAACTGCACTGGATTTGCAGTTAATCGCATGTTTTTTCCATATACTCAATCAGCTCTACTCTTGACAGACCTTGGTTTAGATGTATATCGAATTGATAAAGCGATTACCAGCTTTGGAATGCCTATGGGTCCATTTAGATTAGCTGATCTTGTTGGGTTTGAGGTTGCAGTAGCAACAGGCATGCAGTATCTAGAACATTTTCCTGAACGTGTTTACAAGTCACTGTTGATACCAATTATGCTCGAAGACAAGCGGGCTGGTGAGAAAACTCGTAAAGGTTTCTATTTGTATGATGATAGACGGAAGGCCCGTCCAGACCCTGAAATCAAAAAATATATTGAGAAGTCGAGGAGCATGGCTGGCTTGATTGCTGATGGCAAACCAATTTCCTTGACGGATAAGGATATAGTTGAGATGGTATTCTTTCCAGTTGTCAATGAAGCTTGCCGGGTTCTAGGTGAGGGAATCACGGTTCAAGCTTCAGACCTTGATATTGCCTCTGTAATGGGCATGGGATTCCCACCCTACAGGGGCGGTGTTATGTTCTGGGCAGATTCTCTTGGATCAAATTACATTTATTCCAAATTGAAGATCTGGGCTGAGTCATATGGTGATTTTTTTAAACCCTGCCCATTCTTAGAAGAAAGAGCAGCCACTGGGTCTAAACTGAGTGCCCCAGTAAAAAGTATCAAGTCTCGATTATAATTATGGACAACAGTTCAGAGAATCTCATAAAGAATGTCTGCTGGAGATTCTGGATGAGAGTAATGCTTATTTTTGTATTGGTGATATCTTGTCACCTTACAATTTAATCTCCAGTGATCAGATTGATGATTTATAGTTCAGGGAATTTCGTTGGAGATGACAATGGGATCGTATTGCAGCACAGAGTTTCATCCTGTCAGTCGATATAATATTTGGCTATACAGTATTGGTTGAAACTTGGGTATTAGTGAATATTTCTAGACTAACTCAGGTATTAGCGGGTAGTTCTAGGTTTTTCATTCCTTTACGATGTTTTCAATATATCTGTAGTACGTAAATTTTATTAGTTTATGAATGTTATTGGGCTTGAGTAAAAAAAATGCTTGAAGGATAAAAAGAACCTAATTACGTCTTTTAACAATTGGCCAATATGAGAATTATTCGAGATTACTCTTCATGGTTACTCTTTGTTGGATTAACTGTAAAAACGAAGTGATATATTTGTTAAAATGCATGGAAATAATTCGTATGTTAC

Ace file of the UniGene sp_v2.0_unigene6918 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase [Arabidopsis thaliana] sp\|Q9ZPI5.1\|MFP2_ARATH RecName: Full=Peroxisomal fatty acid beta-oxidation multifunctional protein MFP2; Short=AtMPF2; Includes: RecName: Full=Enoyl-CoA hydratase/3-2-trans-enoyl-C	-	-	69%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	94%
Blast2go	glyoxysomal fatty acid beta-oxidation multifunctional protein mfp-a	-	-	85%

Source	ECs	Term	Type	e value	Identity
Blast2go	Long-chain-enoyl-CoA hydratase.	EC:4.2.1.74	-	0.0	85%

Source	KEGGs	Term	Type	Identity
Blast2go	Fatty acid elongation in mitochondria	00062		85%
Blast2go	Fatty acid metabolism	00071		85%
Blast2go	Metabolic pathways	01100		85%
Blast2go	3-hydroxybutyryl-CoA epimerase.	EC:5.1.2.3	-	85%

Source	KEGGs	Term	Type	Identity
Blast2go	Fatty acid metabolism	00071		85%
Blast2go	Butanoate metabolism	00650		85%
Blast2go	Dodecenoyl-CoA isomerase.	EC:5.3.3.8	-	85%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Fatty acid metabolism	00071		0.0	85%
Blast2go	3-hydroxyacyl-CoA dehydrogenase.	EC:1.1.1.35	-	0.0	85%

Source	KEGGs	Term	Type	Identity
Blast2go	Fatty acid elongation in mitochondria	00062		85%
Blast2go	Fatty acid metabolism	00071		85%
Blast2go	Primary bile acid biosynthesis	00120		85%
Blast2go	Valine, leucine and isoleucine degradation	00280		85%
Blast2go	Geraniol degradation	00281		85%
Blast2go	Lysine degradation	00310		85%
Blast2go	Tryptophan metabolism	00380		85%
Blast2go	Toluene degradation	00623		85%
Blast2go	Butanoate metabolism	00650		85%
Blast2go	Carbon fixation pathways in prokaryotes	00720		85%
Blast2go	Biosynthesis of plant hormones	01070		85%
Blast2go	Metabolic pathways	01100		85%
Blast2go	Biosynthesis of secondary metabolites	01110		85%
Blast2go	Enoyl-CoA hydratase.	EC:4.2.1.17	-	85%

Source	KEGGs	Term	Identity
Blast2go	Fatty acid elongation in mitochondria	00062	85%
Blast2go	Fatty acid metabolism	00071	85%
Blast2go	Valine, leucine and isoleucine degradation	00280	85%
Blast2go	Geraniol degradation	00281	85%
Blast2go	Lysine degradation	00310	85%
Blast2go	Benzoate degradation	00362	85%
Blast2go	Tryptophan metabolism	00380	85%
Blast2go	beta-Alanine metabolism	00410	85%
Blast2go	alpha-Linolenic acid metabolism	00592	85%
Blast2go	Aminobenzoate degradation	00627	85%
Blast2go	Propanoate metabolism	00640	85%
Blast2go	Butanoate metabolism	00650	85%
Blast2go	Limonene and pinene degradation	00903	85%
Blast2go	Biosynthesis of unsaturated fatty acids	01040	85%
Blast2go	Biosynthesis of plant hormones	01070	85%
Blast2go	Metabolic pathways	01100	85%
Blast2go	Biosynthesis of secondary metabolites	01110	85%

Source	GOs	Term	Type	Identity
Blast2go	fatty acid beta-oxidation	GO:0006635	Biological Process	85%
Blast2go	long-chain-enoyl-CoA hydratase activity	GO:0016508	Molecular Function	85%
Blast2go	3-hydroxybutyryl-CoA epimerase activity	GO:0008692	Molecular Function	85%
Blast2go	dodecenoyl-CoA delta-isomerase activity	GO:0004165	Molecular Function	85%
Blast2go	3-hydroxyacyl-CoA dehydrogenase activity	GO:0003857	Molecular Function	85%
Blast2go	coenzyme binding	GO:0050662	Molecular Function	85%
Blast2go	enoyl-CoA hydratase activity	GO:0004300	Molecular Function	85%
Blast2go	3-hydroxyacyl-CoA dehydratase activity	GO:0018812	Molecular Function	85%
Blast2go	cell wall	GO:0005618	Cellular Component	85%
Blast2go	nucleolus	GO:0005730	Cellular Component	85%
Blast2go	glyoxysome	GO:0009514	Cellular Component	85%

Source	InterPros	Term	Type	Identity
Sma3	3-hydroxyacyl-CoA dehydrogenase, NAD binding	IPR006176	-	-
Sma3	3-hydroxyacyl-CoA dehydrogenase, C-terminal	IPR006108	-	-
Sma3	Crotonase, core	IPR001753	-	-
Sma3	Dehydrogenase, multihelical	IPR013328	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NV15

Fln msg:

Fln protein: MGLGRVDMDVGSDGVALITITNPPVNSLSVEVLLGLKEKYDEALQRHDVKAIVITGSSGKFCGGFDIGTLGGLQGAAVQIEGEQFKLTDVSVRIITELFEDAKKPSVAAIDGLALGGGLEIAMACHGRIAAPQAQLGLPELQLGVIPGFGGTQRLPRLVGLSKALEMILLSKPIKSEEASELGLVDAVVPPVELLNTARRWALDIAEFRRPWIRSLYRTDKLEPLGEAREILKFARIQAQKRAANLKHPLVCLDVIEEGIVSGPMAGLLKEVSSFQQLLHSDTAKSLIHVFFAQRSTSKVPGVTDVGLKPRRVRKVAVIGGGLMGSGIATALILSGYPVILKEVNDKFLHSGLERIKANLQSRVNKGKMSQEKFEKILSLVQGTVDYENFRNVDMVIEAVIENVALKQQIFSDLEKHCPSHCVLATNTSTLDLNLIGQKTNSQDRIVGAHFFSPAHIMPLLEIVRTEKTSPQVIVDLMDVGKKIKKTPIVVGNCTGFAVNRMFFPYTQSALLLTDLGLDVYRIDKAITSFGMPMGPFRLADLVGFEVAVATGMQYLEHFPERVYKSLLIPIMLEDKRAGEKTRKGFYLYDDRRKARPDPEIKKYIEKSRSMAGLIADGKPISLTDKDIVEMVFFPVVNEACRVLGEGITVQASDLDIASVMGMGFPPYRGGVMFWADSLGSNYIYSKLKIWAESYGDFFKPCPFLEERAATGSKLSAPVKSIKSRL*

Protein Length: 728

Fln nts: ATGGGGGAACTTCTTAACTTGTGCCTCTTCCTTAATGCATTTGAAAATTTCACTATATACTTTCAAAGGATTTCCTCGCTAAGAAACTGTCCTGGAATATCAACGTTCCCTCTTGCACTTGCTCCTGCTGCGTTCCTTGAAGAATCAATCAGCTGAGTAACATGCTTCTCTAATTTTTGTTTGTTGCGCTGATCGATCCTGGGAATTTTCCTGAGTAGGTCGGTGAAGATTATTTGCAGGAGAAGCT_-_ATGGGCCTTGGAAGAGTTGATATGGATGTGGGATCTGATGGCGTCGCGCTTATAACCATCACAAACCCCCCTGTCAATTCATTGTCAGTTGAAGTCTTGCTAGGCTTGAAAGAGAAATATGATGAAGCTTTGCAAAGACATGACGTGAAAGCAATAGTTATTACAGGTTCTAGTGGCAAATTCTGTGGGGGTTTTGATATAGGAACCTTGGGGGGATTACAAGGTGCGGCTGTACAAATTGAAGGAGAACAGTTTAAATTGACAGATGTTTCAGTCAGAATCATAACCGAATTGTTTGAAGATGCAAAGAAACCGTCGGTTGCTGCTATTGACGGTCTTGCGCTTGGTGGTGGACTTGAGATTGCAATGGCTTGCCATGGACGCATAGCTGCACCACAGGCTCAACTTGGGTTGCCTGAACTCCAACTTGGAGTTATCCCTGGTTTTGGTGGTACACAGCGGCTTCCACGCCTAGTGGGCCTTTCAAAGGCACTTGAAATGATACTTTTGTCGAAACCGATCAAATCTGAAGAGGCTAGTGAGTTGGGGCTTGTGGATGCTGTAGTCCCACCTGTGGAATTGCTAAACACAGCTCGTCGCTGGGCATTGGACATTGCAGAGTTTCGAAGGCCTTGGATAAGAAGCCTTTACCGGACCGACAAGTTAGAACCTTTGGGAGAGGCACGTGAAATCCTAAAATTTGCACGAATTCAAGCACAAAAGAGAGCTGCTAATCTCAAACATCCACTTGTTTGCCTCGATGTCATTGAGGAGGGAATTGTATCTGGACCAATGGCCGGCTTGCTAAAGGAAGTAAGTTCTTTTCAGCAACTTCTTCATTCTGATACCGCCAAGTCATTAATTCATGTTTTCTTTGCACAGCGGTCGACATCAAAGGTTCCTGGAGTCACAGATGTAGGCTTAAAGCCAAGAAGAGTCAGAAAGGTAGCTGTTATTGGTGGGGGTTTGATGGGTTCTGGCATAGCCACAGCCCTAATTCTGAGTGGCTATCCTGTGATCCTTAAAGAAGTAAATGATAAATTCCTTCATTCTGGTCTTGAACGAATTAAAGCAAATTTGCAGAGCCGTGTGAATAAAGGAAAAATGAGTCAAGAAAAGTTTGAGAAAATATTGTCCCTGGTGCAGGGTACAGTTGACTACGAAAATTTTAGGAATGTGGATATGGTAATCGAAGCTGTCATTGAAAATGTAGCTCTGAAACAACAAATATTTAGTGATCTCGAGAAACACTGTCCATCACATTGTGTTCTGGCAACGAATACTTCTACACTCGACTTAAACCTGATTGGCCAGAAAACCAATTCACAGGATCGGATTGTTGGTGCACACTTTTTCAGTCCTGCACATATAATGCCTCTTCTCGAAATAGTGCGCACTGAAAAAACATCCCCCCAAGTTATAGTTGATCTCATGGATGTTGGAAAAAAAATTAAGAAGACACCTATAGTGGTTGGGAACTGCACTGGATTTGCAGTTAATCGCATGTTTTTTCCATATACTCAATCAGCTCTACTCTTGACAGACCTTGGTTTAGATGTATATCGAATTGATAAAGCGATTACCAGCTTTGGAATGCCTATGGGTCCATTTAGATTAGCTGATCTTGTTGGGTTTGAGGTTGCAGTAGCAACAGGCATGCAGTATCTAGAACATTTTCCTGAACGTGTTTACAAGTCACTGTTGATACCAATTATGCTCGAAGACAAGCGGGCTGGTGAGAAAACTCGTAAAGGTTTCTATTTGTATGATGATAGACGGAAGGCCCGTCCAGACCCTGAAATCAAAAAATATATTGAGAAGTCGAGGAGCATGGCTGGCTTGATTGCTGATGGCAAACCAATTTCCTTGACGGATAAGGATATAGTTGAGATGGTATTCTTTCCAGTTGTCAATGAAGCTTGCCGGGTTCTAGGTGAGGGAATCACGGTTCAAGCTTCAGACCTTGATATTGCCTCTGTAATGGGCATGGGATTCCCACCCTACAGGGGCGGTGTTATGTTCTGGGCAGATTCTCTTGGATCAAATTACATTTATTCCAAATTGAAGATCTGGGCTGAGTCATATGGTGATTTTTTTAAACCCTGCCCATTCTTAGAAGAAAGAGCAGCCACTGGGTCTAAACTGAGTGCCCCAGTAAAAAGTATCAAGTCTCGATTATAA___TTATGGACAACAGTTCAGAGAATCTCATAAAGAATGTCTGCTGGAGATTCTGGATGAGAGTAATGCTTATTTTTGTATTGGTGATATCTTGTCACCTTACAATTTAATCTCCAGTGATCAGATTGATGATTTATAGTTCAGGGAATTTCGTTGGAGATGACAATGGGATCGTATTGCAGCACAGAGTTTCATCCTGTCAGTCGATATAATATTTGGCTATACAGTATTGGTTGAAACTTGGGTATTAGTGAATATTTCTAGACTAACTCAGGTATTAGCGGGTAGTTCTAGGTTTTTCATTCCTTTACGATGTTTTCAATATATCTGTAGTACGTAAATTTTATTAGTTTATGAATGTTATTGGGCTTGAGTAAAAAAAATGCTTGAAGGATAAAAAGAACCTAATTACGTCTTTTAACAATTGGCCAATATGAGAATTATTCGAGATTACTCTTCATGGTTACTCTTTGTTGGATTAACTGTAAAAACGAAGTGATATATTTGTTAAAATGCATGGAAATAATTCGTATGTTAC

Fln Alignment:

Contig6918___MGLGRVDMDVGSDGVALITITNPPVNSLSVEVLLGLKEKYDEALQRHDVKAIVITGSSGKFCGGFDIGTLGGLQGAAVQIEGEQFKLTDVSVRIITELFEDAKKPSVXXXXXXXXXXXXXXAMACHGRIAAPQAQLGLPELQLGVIPGFGGTQRLPRLVGLSKALEMILLSKPIKSEEASELGLVDAVVPPVELLNTARRWALDIAEFRRPWIRSLYRTDKLEPLGEAREILKFARIQAQKRAANLKHPLVCLDVIEEGIVSGPMAGLLKEVSSFQQLLHSDTAKSLIHVFFAQRSTSKVPGVTDVGLKPRRVRKVAVIGGGLMGSGIATALILSGYPVILKEVNDKFLHSGLERIKANLQSRVNKGKMSQEKFEKILSLVQGTVDYENFRNVDMVIEAVIENVALKQQIFSDLEKHCPSHCVLATNTSTLDLNLIGQKTNSQDRIVGAHFFSPAHIMPLLEIVRTEKTSPQVIVDLMDVGKKIKKTPIVVGNCTGFAVNRMFFPYTQSALLLTDLGLDVYRIDKAITSFGMPMGPFRLADLVGFEVAVATGMQYLEHFPERVYKSLLIPIMLEDKRAGEKTRKGFYLYDDRRKARPDPEIKKYIEKSRSMAGLIADGKPISLTDKDIVEMVFFPVVNEACRVLGEGITVQASDLDIASVMGMGFPPYRGGVMFWADSLGSNYIYSKLKIWAESYGDFFKPCPFLEERAATGSKLSAPVKSIKSRL
A9NV15______________MGKGGVDIEVGSDGVALITITNPPVNSLSVDVLLGLKEKYDEALQRDDVKAIVITGSGGKFCGGFDIGTLGGLQHGAVKIGGEQFELTDVSVKIITELFEGAKKPSVAAIDGLALGGGLEIAMACHGRIAAPQAQLGLPELQLGVIPGFGGTQRLPRLVGLSKALEMILLSKPIKSEEASELGLVDAVVPPLELLNSARRWALDIAEFRRPWIRSLYRTDKLEPLGEAREILKFARIQAQKRAANLKHPLVCLDVIEEGIVSGPMAGLVKEVSSFQQLLHSDNAKALIHVFFSQRLTSKVPGVTDVGLKPRRVRKVAVLGGGLMGSGIATALILSGYPVILKEVNDRFLQAGLERIKANLQSRVNKGKMSQEKFEKIFSLVHGTVDYENFRNVDIVIEAVIENVALKQQIFSDLEKFCPPHCVLATNTSTIDLSLIGQKTNSQDRIVGAHFFSPAHIMPLLEIVRTEKTSPQVIVDLMDVGKKIKKTPIVVGNCTGFAVNRMFFPYTQSALFLTDLGLDVYRIDIVITSFGMPMGPFRLADLVGFEVAVATGMQYLENFPERVYKSLLIPIMLEDKRAGEKTRKGFYLYDDRRKARPDPEIKKYIEKSRSMAGLIADGKPLSLMDKDIVEMVFFPVVNEACRVLGEGITVQASDLDIASVMGMGFPPYRGGVMFWADSLGSNYIYSKLKIWAESYGDFFKPCPFLEERAATGSKLSAPVKSIKSRL

SNPs (tot: 33)

Position	A %	C %	G %	T %	Probability
366			96	3	0.990463
368	3			94	0.951749
371		96	3		0.991825
373		3		96	0.99245
377		96	4		0.99411
379	2		4	94	0.967557
380		93	2	4	0.976545
381		2	4	93	0.976545
382	4	95			0.995482
385	95		4		0.996252
387		4	95		0.997494
390	95		5		0.997749
391			5	95	0.997749
394	5	92	2		0.992495
399	94	5			0.998578
400		94	5		0.998578
403			6	84	0.999999
404	84		9	6	0.999999
405	90		9		1.0
406	87		12		1.0
408		90	9		1.0
410		9		81	0.999999
413	87	3	9		1.0
414		9	9	81	0.999999
415		93	6		0.999153
416	9			90	1.0
417		6	93		0.999264
418	90		9		1.0
419		6		93	0.999264
420		93	6		0.999364
423			6	83	1.0
425	10		89		1.0
426	88	11			1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain