SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene6527

Length: 2099 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Uagpf v2 Uma v2

UniGene Fasta

>sp_v2.0_unigene6527
AATGTAGATAGTTATAACTGGGGATATGATCCAGTCCTTTGGGGTGTGCCAAAAGGGAGTTATTCAACAAATCCAAATGTTCTCTACAACATTGGAATGCAAGTGGACCAAATGATAAGGACTCTATCCTTGACAAGGTTGTTCCTGGTTATTATTTACGAAGAAACACAGATGGGCTTGTGGAAAACAGTACATGTGTGAACAACACCGCCAGTGAGCATTACATGGTGAATCGATTGATAATTGATGATCTTCTTCTCTGGGCAACAGAGTATAAGGTTGATGGATTTCGGTTTGACCTTATGGGACATCTGATGAAAAATACCATGGTTAGTGCAAGAAATGCACTTGATAGGTTATCAAGGGAGAAAAATGGCGTGGATGGTACTAAGATTTACATGTATGGAGAAGGATGGGATTTTGGTGAGGTTGCACACAATGGACGAGGGATAAATGCATCCCAATTAAATCTCACGGGAACAGGGATTGGGAGTTTTAACGATCGCATACGTGACTCAGTTCTTGGTGGTTCTCCATTTGGTCCTCCTCTTCAACAAGGTTTTGTGACAGGCTTATCTTTACAGCCAAACAAGCACAACCATGGAAATAAGGATGATGTAACAAAGTATCTTGCATCAACAACTGATTGGATTCATGTTGGATTGGCTGGGAATTTAAGAGAGTTTGTATTCACTAACCATGAAGGACTAGAGGTAAAGGGATCAGAAGTGCAAACTCATGATGGAGTACCTGTTGGTTATGCAGGTAGTCCAAAAGAAACAATAAATTATGTTTCTGCACATGATAATGAAACGTTATTCGACATCATCATGCTAAAGGCTGCAAATGAAGTTCCATTGGATGAAAGGTGTAGGATAAATCATTTGGCGACAAGTATTGTGGCACTGTCACAGGGTATACCTTTTTTCCATGCAGGTGATGACATGTTACGCTCTAAATCTCTGGATCGCGATTCATATAACTCTGGTGATTGGTTCAACAGGTTAGACTTCACGTACGAATCAAATAATTGGGGAGTTGGATTGCCTCCAAAAGAAAAGAATGGAAATAATTGGCCTTTGATGAAGGAACTTCTCGGAAATCCATCTTTGAGACCAGGGAAGCATCATATTCTTTCTGCTCTTGGAAACTTCCAAAACCTGTTGAGAATCAGACGGTCATCGCCTCTATTTCGGCTGGGGACAGTAAATGCTATTCAAGCAAGAGTTTGTTTCCACAACACTGGGCCATCATGGATCCCAGGGGCCATTGTTATGAGCATTAAGGATGGTGACAAAGGAATGCCTGGTTTAACTCAGCTTGATCCCTTGTACCGAAGGGTAGTTCTAATATTTAATGCCCGTCCCAGTGAATTTGTTGCTCCAATTTCTGAACTGAAATCATTGCCACTTACCCTCCATCCAATACAGGAGGCATCATCTGATGCACGGGTAAAAACTTCTTTCTATGATGCAAGCACTGGTACATTCAAGATTCCGCCAAGGACAACCTCTGTATTTGTGGAAGCCCGTTAGGCACGTGTCTCCTTTCACTGTTTCTCCATGAATAAGGAAATCAAAGCAGCTATAAAAGCACCCCACTGGTATTTACAGAAACATCATGTAAGTAGCTTCAGGAATATAATGGCATACATTTGGATTGTTTATTTGTGATGCACATCATTTAATTTATCAATTTTGTATTTCAAGACTGGTTATGTAAAATGAACTTTAGTTGGAAATCGGAACAGCAACTGTCAACTACGTTTATGGGGAATAAAACATTCTAAAAGATGCGAGGTTACTCAATTGTGGAGGGAGCATCTGGGTATTGTTTATATTCCTTAATTTAGTGATGGACAATCCCTTATTGACTTGTACCCAGTTTCTGTGAAATAATTGCCGTCGAATTTTCATACTTCTGTCAAAATCCAGGGATCTCACAAAAATTAGCTGCAATGTAATCTTTACGGAAAGGATAACCAATCCAACTCTCAAGCACCAATATATGTTTCAGATATGAATGATTTTCGTTACGAGAACATCTAGCATCAATGAAGTGTAAATCACAAAGCACCAAGGTGATAGCTTTGTGTAACT

Ace file of the UniGene sp_v2.0_unigene6527

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	protein limit dextrinase [Arabidopsis thaliana] sp\|Q8GTR4.2\|PULA1_ARATH RecName: Full=Pullulanase 1, chloroplastic; Short=AtPU1; AltName: Full=Protein LIMIT DEXTRINASE; Short=AtLDA; Flags: Precursor gb\|AED90732.1\| protein limit dextrinase [Arabidopsis tha	-	-	68%
FL-Next	sp=Pullulanase 1, chloroplastic; Arabidopsis thaliana (Mouse-ear cress).	-	-	49%
Blast2go	pullulanase chloroplastic-like	-	-	79%

Source	ECs	Term	Type	Identity
Blast2go	Pullulanase.	EC:3.2.1.41	-	79%
Blast2go	Limit dextrinase.	EC:3.2.1.142	-	79%
Blast2go	Alpha-amylase.	EC:3.2.1.1	-	79%

Source	KEGGs	Term	Type	Identity
Blast2go	Starch and sucrose metabolism	00500		79%
Blast2go	Metabolic pathways	01100		79%
Blast2go	Transferases, Glycosyltransferases, Hexosyltransferases.	EC:2.4.1.-	-	79%

Source	KEGGs	Term	Identity
Blast2go	Fructose and mannose metabolism	00051	79%
Blast2go	N-Glycan biosynthesis	00510	79%
Blast2go	Mucin type O-Glycan biosynthesis	00512	79%
Blast2go	Various types of N-glycan biosynthesis	00513	79%
Blast2go	Other types of O-glycan biosynthesis	00514	79%
Blast2go	Biosynthesis of 12-, 14- and 16-membered macrolides	00522	79%
Blast2go	Glycosaminoglycan biosynthesis - keratan sulfate	00533	79%
Blast2go	Lipopolysaccharide biosynthesis	00540	79%
Blast2go	Peptidoglycan biosynthesis	00550	79%
Blast2go	Glycerolipid metabolism	00561	79%
Blast2go	Glycosylphosphatidylinositol(GPI)-anchor biosynthesis	00563	79%
Blast2go	Sphingolipid metabolism	00600	79%
Blast2go	Glycosphingolipid biosynthesis - lacto and neolacto series	00601	79%
Blast2go	Glycosphingolipid biosynthesis - globo series	00603	79%
Blast2go	Glycosphingolipid biosynthesis - ganglio series	00604	79%
Blast2go	Carotenoid biosynthesis	00906	79%
Blast2go	Zeatin biosynthesis	00908	79%
Blast2go	Flavonoid biosynthesis	00941	79%
Blast2go	Anthocyanin biosynthesis	00942	79%
Blast2go	Flavone and flavonol biosynthesis	00944	79%
Blast2go	Stilbenoid, diarylheptanoid and gingerol biosynthesis	00945	79%
Blast2go	Betalain biosynthesis	00965	79%
Blast2go	Biosynthesis of type II polyketide products	01057	79%
Blast2go	Metabolic pathways	01100	79%
Blast2go	Biosynthesis of secondary metabolites	01110	79%

Source	GOs	Term	Type	Identity
Blast2go	starch biosynthetic process	GO:0019252	Biological Process	79%
Blast2go	starch catabolic process	GO:0005983	Biological Process	79%
Blast2go	pullulanase activity	GO:0051060	Molecular Function	79%
Blast2go	limit dextrinase activity	GO:0010303	Molecular Function	79%
Blast2go	cation binding	GO:0043169	Molecular Function	79%
Blast2go	alpha-amylase activity	GO:0004556	Molecular Function	79%
Blast2go	transferase activity, transferring hexosyl groups	GO:0016758	Molecular Function	79%
Blast2go	chloroplast	GO:0009507	Cellular Component	79%

Source	InterPros	Term	Type	Identity
Sma3	Regulator of chromosome condensation, RCC1	IPR000408	-	-
Sma3	Glycoside hydrolase, family 13, N-terminal	IPR004193	-	-
Sma3	Bacterial pullanase-associated domain	IPR005323	-	-
Sma3	Glycosyl hydrolase, family 13, catalytic domain	IPR006047	-	-
Sma3	Alpha-1,6-glucosidases, pullulanase-type	IPR011839	-	-
Sma3	Glycoside hydrolase, subgroup, catalytic domain	IPR013781	-	-
Sma3	Immunoglobulin-like fold	IPR013783	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: sp_plants

Fln subject: Q8GTR4

Fln msg: Warning!, your query overlaps and the subject is separated, your sequence is shorter than subject: 512 - 965

Fln protein: NVDSYNWGYDPVLWGVPKGSYSTNPNVLYxxxxxxxxxxxxxxxxxxxxxxxLRRNTDGLVENSTCVNNTASEHYMVNRLIIDDLLLWATEYKVDGFRFDLMGHLMKNTMVSARNALDRLSREKNGVDGTKIYMYGEGWDFGEVAHNGRGINASQLNLTGTGIGSFNDRIRDSVLGGSPFGPPLQQGFVTGLSLQPNKHNHGNKDDVTKYLASTTDWIHVGLAGNLREFVFTNHEGLEVKGSEVQTHDGVPVGYAGSPKETINYVSAHDNETLFDIIMLKAANEVPLDERCRINHLATSIVALSQGIPFFHAGDDMLRSKSLDRDSYNSGDWFNRLDFTYESNNWGVGLPPKEKNGNNWPLMKELLGNPSLRPGKHHILSALGNFQNLLRIRRSSPLFRLGTVNAIQARVCFHNTGPSWIPGAIVMSIKDGDKGMPGLTQLDPLYRRVVLIFNARPSEFVAPISELKSLPLTLHPIQEASSDARVKTSFYDASTGTFKIPPRTTSVFVEAR*

Protein Length: 513

Fln nts: AATGTAGATAGTTATAACTGGGGATATGATCCAGTCCTTTGGGGTGTGCCAAAAGGGAGTTATTCAACAAATCCAAATGTTCTCTACxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxTTACGAAGAAACACAGATGGGCTTGTGGAAAACAGTACATGTGTGAACAACACCGCCAGTGAGCATTACATGGTGAATCGATTGATAATTGATGATCTTCTTCTCTGGGCAACAGAGTATAAGGTTGATGGATTTCGGTTTGACCTTATGGGACATCTGATGAAAAATACCATGGTTAGTGCAAGAAATGCACTTGATAGGTTATCAAGGGAGAAAAATGGCGTGGATGGTACTAAGATTTACATGTATGGAGAAGGATGGGATTTTGGTGAGGTTGCACACAATGGACGAGGGATAAATGCATCCCAATTAAATCTCACGGGAACAGGGATTGGGAGTTTTAACGATCGCATACGTGACTCAGTTCTTGGTGGTTCTCCATTTGGTCCTCCTCTTCAACAAGGTTTTGTGACAGGCTTATCTTTACAGCCAAACAAGCACAACCATGGAAATAAGGATGATGTAACAAAGTATCTTGCATCAACAACTGATTGGATTCATGTTGGATTGGCTGGGAATTTAAGAGAGTTTGTATTCACTAACCATGAAGGACTAGAGGTAAAGGGATCAGAAGTGCAAACTCATGATGGAGTACCTGTTGGTTATGCAGGTAGTCCAAAAGAAACAATAAATTATGTTTCTGCACATGATAATGAAACGTTATTCGACATCATCATGCTAAAGGCTGCAAATGAAGTTCCATTGGATGAAAGGTGTAGGATAAATCATTTGGCGACAAGTATTGTGGCACTGTCACAGGGTATACCTTTTTTCCATGCAGGTGATGACATGTTACGCTCTAAATCTCTGGATCGCGATTCATATAACTCTGGTGATTGGTTCAACAGGTTAGACTTCACGTACGAATCAAATAATTGGGGAGTTGGATTGCCTCCAAAAGAAAAGAATGGAAATAATTGGCCTTTGATGAAGGAACTTCTCGGAAATCCATCTTTGAGACCAGGGAAGCATCATATTCTTTCTGCTCTTGGAAACTTCCAAAACCTGTTGAGAATCAGACGGTCATCGCCTCTATTTCGGCTGGGGACAGTAAATGCTATTCAAGCAAGAGTTTGTTTCCACAACACTGGGCCATCATGGATCCCAGGGGCCATTGTTATGAGCATTAAGGATGGTGACAAAGGAATGCCTGGTTTAACTCAGCTTGATCCCTTGTACCGAAGGGTAGTTCTAATATTTAATGCCCGTCCCAGTGAATTTGTTGCTCCAATTTCTGAACTGAAATCATTGCCACTTACCCTCCATCCAATACAGGAGGCATCATCTGATGCACGGGTAAAAACTTCTTTCTATGATGCAAGCACTGGTACATTCAAGATTCCGCCAAGGACAACCTCTGTATTTGTGGAAGCCCGTTAGGCACGTGTCTCCTTTCACTGTTTCTCCATGAATAAGGAAATCAAAGCAGCTATAAAAGCACCCCACTGGTATTTACAGAAACATCATGTAAGTAGCTTCAGGAATATAATGGCATACATTTGGATTGTTTATTTGTGATGCACATCATTTAATTTATCAATTTTGTATTTCAAGACTGGTTATGTAAAATGAACTTTAGTTGGAAATCGGAACAGCAACTGTCAACTACGTTTATGGGGAATAAAACATTCTAAAAGATGCGAGGTTACTCAATTGTGGAGGGAGCATCTGGGTATTGTTTATATTCCTTAATTTAGTGATGGACAATCCCTTATTGACTTGTACCCAGTTTCTGTGAAATAATTGCCGTCGAATTTTCATACTTCTGTCAAAATCCAGGGATCTCACAAAAATTAGCTGCAATGTAATCTTTACGGAAAGGATAACCAATCCAACTCTCAAGCACCAATATATGTTTCAGATATGAATGATTTTCGTTACGAGAACATCTAGCATCAATGAAGTGTAAATCACAAAGCACCAAGGTGATAGCTTTGTGTAACT

Fln Alignment:

Contig6527___NVDSYNWGYDPVLWGVPKGSYSTNPNVLYNIGMQVDQMIRTLSLTRLFLVIxxxxxHWNASGPNDKDSILDKVVPGYYLRRNTDGLVENSTCVNNTASEHYMVNRLIIDDLLLWATEYKVDGFRFDLMGHLMKNTMVSARNALDRLSREKNGVDGTKIYMYGEGWDFGEVAHNGRGINASQLNLTGTGIGSFNDRIRDSVLGGSPFGPPLQQGFVTGLSLQPNKHNHGNKDDVTKYLASTTDWIHVGLAGNLREFVFTNHEGLEVKGSEVQTHDGVPVGYAGSPKETINYVSAHDNETLFDIIMLKAANEVPLDERCRINHLATSIVALSQGIPFFHAGDDMLRSKSLDRDSYNSGDWFNRLDFTYESNNWGVGLPPKEKNGNNWPLMKELLGNPSLRPGKHHILSALGNFQNLLRIRRSSPLFRLGTVNAIQARVCFHNTGPSWIPGAIVMSIKDGDKGMPGLTQLDPLYRRVVLIFNARPSEFVAPISELKSLPLTLHPIQEASSDARVKTSFYDASTGTFKIPPRTTSVFVEAR
Q8GTR4______________NDDGYNWGYNPVLWGVPKGSYASDPTGPCRI-IEFRKMVQALNCTGLNVVLxxxxxHLHASGPHDKESVLDKIVPGYYLRRNSDGFIENSTCVNNTASEHYMVDRLIRDDLLNWVVNYKVDGFRFDLMGHIMKATIVNAKSAIGSLRKETDGVDGSRIYLYGEGWNFGEVAENGRGINASQFNLGGTGIGSFNDRIRDATLGGSPFGHPLQQGFITGLLLQPNAHDHGSEATQELMLSTAKNHIQTGMAANLKDYMLTNHEGKEVKGSEVLMHDATPVAYASLPTETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLASSMIALSQGIPFFHAGDEILRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKGKNEHNWPLIKPRLQDPSFKPKSSHIVATLHNFLDLLRIRYSSPLFRLDTARAIQERVRFHNTGPSSIPGAIVMSIEDGHRGIPSVSQIDPIYSLIVVIFNARPSEFSYPSPALKDRKLELHPVQVMSADEIVKKSVYDSFSGGFTVPARTTTVFVESR

SNPs (tot: 61)

Position	A %	C %	G %	T %	Probability
905	75		25		0.996473
906			25	75	0.996473
907			25	75	0.996473
930		83	16		0.994512
931	16			83	0.994512
932		16		83	0.994512
933			16	83	0.994512
935			16	83	0.994512
936	16			83	0.994512
939	83			16	0.994512
942		83	16		0.994512
943	83	16			0.994512
944			83	16	0.994512
947		16	83		0.994512
949		16		83	0.994512
950			83	16	0.994512
1113	20	80			0.995493
1117		83		16	0.994512
1118			20	80	0.995493
1120		20		80	0.995493
1122	80			20	0.995493
1127	80			20	0.995493
1128			80	20	0.995493
1131	80	20			0.995493
1132	80			20	0.995493
1140	80	20			0.995493
1141	20			80	0.995493
1216		25		75	0.996473
1270	25	75			0.996473
1363	25			75	1.0
1386		11		88	0.923564
1389		11		88	0.923564
1391		88		11	0.923564
1392			11	88	0.923564
1393	11	88			0.923564
1395	88	11			0.923564
1396	88			11	0.923564
1398			11	88	0.923564
1399			11	88	0.923564
1403	88			11	0.923564
1407		11	88		0.923564
1436		11		88	0.923564
1509	11		88		0.923564
1510		88		11	0.923564
1511		88	11		0.923564
1512	88		11		0.923564
1513	88		11		0.923564
1530		10		90	0.90298
1534			90	10	0.90298
1535	90		10		0.90298
1537		10	90		0.90298
1539		90		10	0.90298
1540		90		10	0.90298
1542			10	90	0.90298
1543		10		90	0.90298
1544	90			10	0.90298
1546			90	10	0.90298
1550		10	90		0.90298
1551	10			90	0.90298
1808	70			30	1.0
2008			42	57	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain