SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene6413

Length: 1378 nt

Origin of reads: Biogeco1 v2 Uagpf v2 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene6413

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Probable protein phosphatase 2C 68 n=3 Tax=Brassicaceae RepID=P2C68_ARATH	-	-	45%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	42%
Blast2go	protein phosphatase 2c 68	-	-	64%

Source	GOs	Term	Type	e value	Identity
Blast2go	hydrolase activity	GO:0016787	Molecular Function	0.0	64%

Source	InterPros	Term	Type	Identity
Sma3	Protein phosphatase 2C, manganese/magnesium aspartate binding site	IPR000222	-	-
Sma3	Zinc finger, RING-type	IPR001841	-	-
Sma3	Protein phosphatase 2C-like	IPR001932	-	-
Sma3	Sugar transporter, conserved site	IPR005829	-	-
Sma3	Beta tubulin, autoregulation binding site	IPR013838	-	-
Sma3		IPR014045	-	-
Sma3	Protein phosphatase 2C	IPR015655	-	-
Sma3	Zinc finger, C3HC4 RING-type	IPR018957	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5AA04

Fln msg: Separated hits, possible frame ERROR between 841 and 849, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 339 - 370

Fln protein: MAMFRGCLFRGGINDKSVDSQELASHRYGKYSVAIARANEPIEDQSEVVKANWGTFVGVYDGHGGTGASMYLKDHLLKDIEEMASEAGDMSAEILDRSIKGAEEGFLKLASQSVEREPNLCSAGSCCVVGYITSKMLFVANVGNCRAVLGITTGDKSIKAERLTKDHSAAVEEIRRELQALHPDDPNIVVFKDNSWLVKGIIEVSRSIGNLYLKKPWLNRNRNLIYGESKEKTFLSAEPYVHGREIQExxxxFIIFASDGLWEHFSDNEAAEIVHSYPAKGIARRLVRGALDKAAKMRGFINYEDLKQNVDRAAMRDVHDDITVAVVFLDRRAVTVHR*

Protein Length: 340

Fln nts: CGTCGTGACCTTGGCTGTCATGGCATATCATTTCTCTCATTTCTCAGGTAGAAATTTCGGAGCCCTTTGAAAACAATAATACTTTCTCTGTATA_-_ATGGCGATGTTCAGAGGTTGCTTGTTTCGCGGTGGAATTAATGACAAGAGCGTCGATTCTCAGGAGTTGGCATCGCATCGATATGGAAAGTATTCAGTAGCAATAGCGCGGGCAAACGAACCCATAGAAGACCAGAGCGAAGTGGTGAAGGCGAATTGGGGTACTTTTGTTGGGGTTTATGACGGCCATGGCGGCACTGGTGCATCAATGTATCTCAAGGATCATCTCCTCAAGGATATTGAAGAGATGGCATCAGAAGCTGGAGACATGTCCGCAGAGATCCTTGATCGATCGATCAAAGGCGCAGAAGAAGGATTTTTGAAATTGGCCAGCCAGAGTGTGGAAAGAGAACCAAACCTGTGCAGCGCTGGTTCTTGTTGCGTGGTCGGTTATATAACAAGCAAAATGCTTTTTGTAGCAAACGTTGGAAACTGCAGAGCTGTTTTAGGAATTACTACTGGGGACAAATCAATAAAGGCAGAACGATTGACCAAAGATCACAGTGCAGCCGTGGAGGAGATTCGCAGAGAGCTTCAAGCACTCCATCCAGATGATCCCAATATAGTAGTTTTCAAGGACAATTCTTGGTTGGTTAAAGGCATAATAGAGGTTTCGAGGTCCATTGGAAATTTATACTTGAAAAAGCCGTGGCTTAACAGAAACCGGAATTTGATATATGGAGAGTCCAAAGAGAAAACCTTTTTATCAGCAGAGCCTTATGTTCATGGGCGAGAAATCCAGGAACAxxxxxxxxxATTCATAATATTTGCATCGGATGGCCTCTGGGAGCATTTCTCTGACAACGAAGCCGCAGAGATCGTGCACAGTTATCCTGCCAAGGGAATAGCCAGGCGGTTAGTTCGAGGTGCTCTTGACAAGGCTGCGAAGATGCGAGGCTTCATCAACTATGAAGACTTGAAGCAGAATGTGGACAGAGCGGCAATGCGAGACGTTCACGATGACATCACTGTGGCAGTTGTGTTCTTAGATCGCAGGGCTGTCACTGTTCATAGATAA___AAGCTCCGAGAAAAAACAAGAATGGCAGCACTTCTGAGGTAGAAATCACATGGATATTTGATGTGTCAGGACTTTTCTGCATAGCTGTAATAACTAGATAATAAAAATCTGAGGCAGAGCATCTTCCATCTTCCGGAACTTGTATCGGGTGTGGACAAGAAATTTGAGCAGCAGTCTCAAAATATTCGAACAAACAAAATGATAGAAATGGATTAAAATTTTGATTTTTCATGTTATAATGGATAATAATGAGATTCATTATCTTATTA

Fln Alignment:

Contig6413___QELASHRYGKYSVAIARANEPIEDQSEVVKANWGTFVGVYDGHGGTGASMYLKDHLLKDIEEMASEAGDMSAEILDRSIKGAEEGFLKLASQSVEREPNLCSAGSCCVVGYITSKMLFVANVGNCRAVLG-ITTGDKSIKAERLTKDHSAAVEEIRRELQALHPDDPNIVVFKDNSWLVKGIIEVSRSIGNLYLKKPWLNR---NRNLIYGESKEKTFLSAEPYVHGREIQEHxxxFIIFASDGLWEHFSDNEAAEIVHSYPAKGIARRLVRGALDKAAKMRGFINYEDLKQNVDRAAMRDVHDDITVAVVFLDRRAVT
D5AA04______________KDIGEHVAGEFSMAVAQANQLVEDQCQVEIGPFGTFVGVYDGHGGPDAARFINDHLFRHFQNFALEQRGMSAEVIRNAFLATEEGFLSLVTNAWPTKPQLAAVGSCCLVGLVYEKTLYVANLGDSRVVMGRLIRATGEIAAVQLSAEHNASMEAVRQELRSSHPDDPQIVVLKHDVWRVKGIIQVSRSIGDAYLKRPEFNREPLNPKFRLPEPLRRPVLTAEPSITTYTLQPHxxxFLIFASDGLWEHLSNQEAVDIVHNHPHVGSAKRLIKAALHEAARKRE-MRYSDLKR-IDRGVRRHFHDDITVIVVFLDHDLIS

SNPs (tot: 21)

Position	A %	C %	G %	T %	Probability
614	25		75		0.996473
616		25	75		0.996473
619			75	25	0.996473
622		25	75		0.996473
623	75	25			0.996473
626		75	25		0.996473
629	75			25	0.996473
631	75			25	0.996473
632		25	75		0.996473
638	25			75	0.996473
820		83	16		0.994512
821	83		16		0.994512
1074	90		10		0.90298
1081		44		55	1.0
1088	11		88		0.923564
1110	88		11		0.923564
1202		50		50	1.0
1228	50		50		1.0
1262	75	25			0.996473
1267		25		75	0.996473
1327			37	62	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain