SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene6139

Length: 1603 nt

Origin of reads: Biogeco1 v2 Uagpf v2 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene6139

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	tRNA (guanine-N-7) methyltransferase [Arabidopsis thaliana] gb\|AAM67168.1\| unknown [Arabidopsis thaliana] gb\|AAO29957.1\| putative protein [Arabidopsis thaliana] gb\|AAP42724.1\| At5g17660 [Arabidopsis thaliana] gb\|AED92453.1\| tRNA (guanine-N-7) methyltransf	-	-	62%
FL-Next	tr=Putative uncharacterized protein; Vitis vinifera (Grape).	-	-	50%
Blast2go	protein	-	-	77%

Source	ECs	Term	Type	e value	Identity
Blast2go	tRNA (guanine-N(7)-)-methyltransferase.	EC:2.1.1.33	-	0.0	77%

Source	GOs	Term	Type	Identity
Blast2go	tRNA modification	GO:0006400	Biological Process	77%
Blast2go	tRNA (guanine-N7-)-methyltransferase activity	GO:0008176	Molecular Function	77%
Blast2go	mitochondrion	GO:0005739	Cellular Component	77%

Source	InterPros	Term	Type	e value	Identity
Sma3	tRNA (guanine-N-7) methyltransferase	IPR003358	-	0.0	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: tr_plants

Fln subject: D7UB80

Fln msg: Overlapping hits, possible frame ERROR between 730 and 729, Unexpected STOP codon in 5 prime region,

Fln protein: MAFTGTRSIATSTWLHPTRKTPLSKFFRVGRFATGQSSSHHFCSSTCQSPRSSRTDTAASNTVKGDHSIPKSGKQRNPQLVDMEYAELNLNEHVGQFGAVRVRQHVNPLKAAFMVPLEPPNWEDVFEDTRLPLMVDIGSGSGRFLILLAKRFLGSKNFLGLDIREKLVERSQFWAKELTLANTHFMVANATFTFGTLVST*xxGPLTCVSILCPDPHFKKRHHKRRILQEPLVDAIINNLACGGQILVQSDVHEVAVDMRKHFDSRFDSVQHMDAHHPNLCDDDGWLLENPIGIRTEREILAQSEGAKIYRRMYEKM*

Protein Length: 319

Fln nts: CGTCGTGACATGGGAGAATTTTCATTCTCCGTTCATGCTTTGCAGGGACCATGCAACATAATTGAAAAAACATATGAAATCCTGCGACACTGTCAAACGTACACGTAAGTGATTGCTCAAGTAA_-_ATGGCATTCACAGGGACCCGAAGCATTGCAACTTCAACATGGCTCCATCCCACTCGGAAAACACCTCTGAGCAAATTCTTCCGTGTTGGGCGATTTGCCACTGGCCAAAGCTCCTCTCACCATTTTTGCTCTTCCACGTGCCAATCTCCTCGCTCTTCACGCACTGACACGGCGGCATCCAACACAGTGAAAGGCGACCATAGTATACCAAAGAGTGGCAAGCAAAGGAACCCACAGCTGGTGGACATGGAGTATGCAGAGCTCAACCTCAACGAACATGTGGGACAGTTTGGAGCTGTGCGAGTCCGGCAGCATGTTAACCCACTGAAGGCAGCTTTCATGGTTCCTTTGGAACCACCAAATTGGGAAGATGTTTTCGAAGACACAAGACTACCCCTCATGGTGGATATTGGAAGTGGCAGTGGCAGGTTTCTTATTTTGCTTGCGAAAAGGTTCCTAGGATCAAAGAATTTTCTGGGACTGGACATACGAGAAAAGTTGGTAGAACGTTCACAGTTCTGGGCGAAGGAATTGACACTTGCAAACACACATTTCATGGTTGCCAATGCTACATTTACTTTTGGAACTTTGGTTTCAACGTGAxxxxxxGGTCCTTTGACATGTGTTTCAATCTTGTGTCCTGATCCACATTTCAAGAAAAGACATCACAAACGAAGGATTCTTCAGGAGCCATTAGTAGATGCAATTATAAATAATTTGGCTTGTGGAGGTCAGATATTAGTTCAGTCTGATGTTCATGAAGTGGCAGTTGACATGAGGAAACATTTTGATAGCCGTTTTGATTCTGTCCAGCACATGGATGCCCATCACCCGAATTTGTGTGATGATGATGGGTGGCTCTTAGAGAATCCAATTGGTATAAGGACAGAAAGAGAGATTCTTGCTCAGTCTGAAGGTGCAAAAATCTATCGAAGAATGTATGAAAAGATGTGA___ACTATGATGTTACTGACCAGAATCTACTGCTTATGTCACCGTCAGATCATGAAGTTTCTATATTATGGCGTTTCAATCCCACGTGCTTTTACAGACAAACATGTTTTCCTTGGAGTAGATGATACTATGGTTAGATGACATAAGGCCCATGCCAATGGGGTTCTTTGTGAAAATACGCTCATCAAAAGCTGGGTTTTATTTACAGTTATCATATTGGAAACAGATTATCAGAATTTACAGGGAGAGAATAAAGGAATTGTGCATCTAATTTATCTTGAAGTAAGTTGCCATTCCCTTCTTTCTGTTGCTTTGTTGGAAGCAATAACGAAGTTGATCTCTGAGAGAAAAGGGAAAAAATTCTGCAAATTTTCTGGGTACCAATGTATAGCTCTTTAAACATAAGAATCTAGTTGGCGATTTTGCTTAACCACACACAAGGAATCTCTAATTTATATCTCCTTATGAGATTCGTTATGTAGCATTTCCATTGCACATACAGTATACTTGGTGCAAGAATTAGTTAATGC

Fln Alignment:

Contig6139___ATGQSSSHHFCSSTCQSPRSSRTDTAASNTVKGDHSIPKSGKQRNPQLVDMEYAELNLN-EHVGQ-FGAVRVRQHVNPLKAAFMVPLEPPNWEDVFEDTRLPLMVDIGSGSGRFLILLAKRFLGSKNFLGLDIREKLVERSQFWAKELTLANTHFMVANATFTFGTLVST*xGPLTCVSILCPDPHFKKRHHKRRILQEPLVDAIINNLACGGQILVQSDVHEVAVDMRKHFDSRFDSVQHMDAHHPNL-CDDDGWLLENPIGIRTEREILAQSEGAKIYRRMYEKM
D7UB80______________SSSSSSIFHHHRHFCNSP------TAVASTAK---------QLTSPELVALEYADLNLNYDNVSQEVGHVRIRQHVNPFAPSFSIPAPVPNWCQVFKDSTLPLMVDIGSGSGRFLMWLAKRNPDSQNYLGLEIRQKLAKRAEFWVKELSLHNIHFMFANATISFKELVSAYxGPLMLVSLLCPDPHFKKRHHKRRVVQKPLVDSIVKNLMPGGQVFIQSDVLEVAVDMRDRFDAESDLLQHIDTIDPSMQCDEEGWLVKNPMGIRTEREIHAEFEGAKIYRRLYQKL

SNPs (tot: 35)

Position	A %	C %	G %	T %	Probability
10	66	33			0.997452
12			33	33	0.997753
15		33	66		0.997452
16	66			33	0.997452
18	66			33	0.997452
19	66	33			0.997452
20	33			66	0.997452
21		33		66	0.997452
23		33		66	0.997452
24	33	66			0.997452
45	66	33			0.997452
51	33	66			0.997452
93	33		66		0.997452
186	75		25		0.996473
268	75		25		0.996473
606		66		33	0.997452
746	75		25		0.996473
893	54	27	18		1.0
995		10		90	0.90298
1108			80	20	0.995493
1124		14	85		0.993533
1125	83		16		0.994512
1128	83		16		0.994512
1133		16	83		0.994512
1134		16		83	0.994512
1137		83	16		0.994512
1138			16	83	0.994512
1139	83	16			0.994512
1141	83		16		0.994512
1143		16		83	0.994512
1145		16		83	0.994512
1146			83	16	0.994512
1147		16	83		0.994512
1148	16	83			0.994512
1273		50		50	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain