SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene6133

Length: 2266 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Uagpf v2 Uma v2

UniGene Fasta

>sp_v2.0_unigene6133
CGTCGTGACCTTGGCTGTCACTCATATCTCATTCCCTCAACTTGGTTACATACAATACAATTCAATCAAATCAAAATGCAAGGATTCTTGCATTCCCATTGTTCAATATGTCATGGCTGTTAATAGTGGATTTTGATCGGGTTAAAACATGCAAAGAGTCATTGTATAATCTACCCTGCACAACAAGAAGAACAGGGTAGAAGAGGATTGTTGTCGTTAAGAGTGGTAACCAGTTAGCGCTAGCAGGGCTCTCTGTTGGCTCTGTGCACATTTGTTGTACTTTGCTTTTCGTCATGGAAAACTCAACTTTGGGCTTTATTTGTCACATACTATTCTTTATAGCTCTTGGCTTACGGTATTCGAATGGGCAATCCTACAGTTTGCAGATGTATCATAAATTTTCTCCTGAAGTAAAAGAGTGGATGACATGGAGACATGGATTAGATACAGATGGGTGGCCTACGGAGGGAAGGAACGAGTATTACAAAGCATTGTATCATCATGATTCTGCTCGCCATGATCGTAAATTGGGCGACGTACCCGTCGCTTACATTTCTAGCAGGCAACATGACAAACTCATTTTCACAGTTGGGATATTTGTTCTACACCATGGTTCAAGTGGGCACTCCCAACGTAACCTTACTTGTAGCACTTGACACTGGCAGTGATTTGTTCTGGGTGCCTTGTGATTGCCAGGAGTGTGCTCCTACTTCTGCACCTTACTATGGATTAGATATCAATATCAATTTACAGAGTTACAGTCCATCAGCCTCACAGACAAGCAAGCCAGTTACTTGTGATAATACTCTGTGTGATTTACAAAAGAAATGCTCCAAATCTAGTGATCAATGCCCATACAACGTCGCATATGTCTCTGCAAATACTTCCACTTCAGGAACTTTAGTGGAGGATATTCTTTATCTGATTCCTGGAAATGGGTCCCGGAATGGAACAGTAGTGAAGGCACCTATCACCTTCGGGTGTGGAAAGGTACAAACGGGCTCATTTCTACAAGGTGCAGCACCATATGGTCTCACTTGGGCTTGGCGTTGAAACCATCTCTGTCACTCATATTTTATATAAATCAGGCCTTGTATCAAATTCTTTCTCTATGTGCTTCCCTCTGAATTCATCTGTCGGAAGGTTTACCTTTGGAGATAAAGTGACTTCAGACCAAAAGGAAACCCCTTTTATTATTGATCAAACACACCCAACCTATTATTTAGGTGTGAAGGAATTTTACGTGGGAACCGCTCTCGTCAAAACAGAATTTCAAGCCCTTTTTGACACTGGCACCTCATTTACATACTTGGTTGATTCAGTTTACAAAAGCCTTACATCAAATTATCATCTGCAGAGGAAAGCCTCTCCATTGGCAATTGATGATTCAAACATTCCTTTCGAGTTTTGTTATGAAATCAGCAACAGTCAAAGCATTAATCAAGGGCCTAATATATCTTTCAACTTCAATGGAGGAAACAACTTTTCTGCTATTCAACCATTAGTCCTTATTGGTGATGAGAATGGGAAGTTGGTAGGTTACTGTTTGGCAGTGCTTCAGAGCTCGACAGTCACAATTATTGGTCAGAATTTCATGGCAGGTTATCAACTTGTCTTTGACCGGGAGCAGCTCAAACTGGGGTGGAAGGAAGCTAACTGTAATGATTTGGAGTATAGCAGCAGTGCAGCGTCAGCACCATCAAGTTTGACACCACTTCAGTCACCCCTTGCCCCTTCCTCGGTCGATGACAGCGAACACAAATCTAATACAACTACAAATAGTTCAACAGGGTCACCTCCGAAGCAGTCCATATCTCCTGATGCTCTACCAGCTAATCCTAATGCCATCGGGAATGATGCAACCCATTTAAGCTTATTTTTTCTGTGCAATGTCATCTCATTTACAGTCACCATCACCATTCTTACAAATCTCTTTTAAATCATTTATGCACAATGAAGATGTCTATGGTGACATGAGTTTTTCCTTCCCATTATTTTTATTGTTCCAATTTTTATAAAGTCACCATAGAAGTTCCATATTTAATCATCTTTACAGCTGTAAAGATGTACAGTTTTAATCATGAAAGAAGTAATAGGCCGTTCATGTACAGCAGAAGTTCCATTGTTGTAACTATGATTAGCATCAAACTTCAGTTTTTGTTTTAACTTTAATTAGGAATAGTGGAGAAGGATTTTTGATGTATGTGGTGATATATGTGTCCTATATGAATGAATTTGATTTGAGATTGTGGGCATTTAAATTTCG

Ace file of the UniGene sp_v2.0_unigene6133

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	aspartyl protease family protein [Arabidopsis thaliana] gb\|ACE79041.1\| At4g35880 [Arabidopsis thaliana] dbj\|BAH19546.1\| AT4G35880 [Arabidopsis thaliana] gb\|AEE86584.1\| aspartyl protease family protein [Arabidopsis thaliana]	-	-	48%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	75%
Blast2go	aspartyl protease family protein	-	-	67%

Source	GOs	Term	Type	Identity
Blast2go	proteolysis	GO:0006508	Biological Process	67%
Blast2go	hydrolase activity	GO:0016787	Molecular Function	67%
Blast2go	anchored to membrane	GO:0031225	Cellular Component	67%
Blast2go	cytoplasmic membrane-bounded vesicle	GO:0016023	Cellular Component	67%

Source	InterPros	Term	Type	Identity
Sma3	Peptidase A1	IPR001461	-	-
Sma3	Peptidase aspartic, active site	IPR001969	-	-
Sma3	DNA/RNA-binding protein Alba-like	IPR002775	-	-
Sma3	Peptidase aspartic, catalytic	IPR009007	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5A9P2

Fln msg: Overlapping hits, possible frame ERROR between 542 and 532, your sequence is longer than subject: 254 - 165

Fln protein: MENSTLGFICHILFFIALGLRYSNGQSYSLQMYHKFSPEVKEWMTWRHGLDTDGWPTEGRNEYYKALYHHDSARHDRKLxxxxxSLTFLAGNMTNSFSQLGYLFYTMVQVGTPNVTLLVALDTGSDLFWVPCDCQECAPTSAPYYGLDININLQSYSPSASQTSKPVTCDNTLCDLQKKCSKSSDQCPYNVAYVSANTSTSGTLVEDILYLIPGNGSRNGTVVKAPITFGCGKVQTGSFLQGAAPYGLTWAWR*

Protein Length: 255

Fln nts: CGTCGTGACCTTGGCTGTCACTCATATCTCATTCCCTCAACTTGGTTACATACAATACAATTCAATCAAATCAAAATGCAAGGATTCTTGCATTCCCATTGTTCAATATGTCATGGCTGTTAATAGTGGATTTTGATCGGGTTAAAACATGCAAAGAGTCATTGTATAATCTACCCTGCACAACAAGAAGAACAGGGTAGAAGAGGATTGTTGTCGTTAAGAGTGGTAACCAGTTAGCGCTAGCAGGGCTCTCTGTTGGCTCTGTGCACATTTGTTGTACTTTGCTTTTCGTC_-_ATGGAAAACTCAACTTTGGGCTTTATTTGTCACATACTATTCTTTATAGCTCTTGGCTTACGGTATTCGAATGGGCAATCCTACAGTTTGCAGATGTATCATAAATTTTCTCCTGAAGTAAAAGAGTGGATGACATGGAGACATGGATTAGATACAGATGGGTGGCCTACGGAGGGAAGGAACGAGTATTACAAAGCATTGTATCATCATGATTCTGCTCGCCATGATCGTAAATTGxxxxxxxxxxxxxxxTCGCTTACATTTCTAGCAGGCAACATGACAAACTCATTTTCACAGTTGGGATATTTGTTCTACACCATGGTTCAAGTGGGCACTCCCAACGTAACCTTACTTGTAGCACTTGACACTGGCAGTGATTTGTTCTGGGTGCCTTGTGATTGCCAGGAGTGTGCTCCTACTTCTGCACCTTACTATGGATTAGATATCAATATCAATTTACAGAGTTACAGTCCATCAGCCTCACAGACAAGCAAGCCAGTTACTTGTGATAATACTCTGTGTGATTTACAAAAGAAATGCTCCAAATCTAGTGATCAATGCCCATACAACGTCGCATATGTCTCTGCAAATACTTCCACTTCAGGAACTTTAGTGGAGGATATTCTTTATCTGATTCCTGGAAATGGGTCCCGGAATGGAACAGTAGTGAAGGCACCTATCACCTTCGGGTGTGGAAAGGTACAAACGGGCTCATTTCTACAAGGTGCAGCACCATATGGTCTCACTTGGGCTTGGCGTTGA___AACCATCTCTGTCACTCATATTTTATATAAATCAGGCCTTGTATCAAATTCTTTCTCTATGTGCTTCCCTCTGAATTCATCTGTCGGAAGGTTTACCTTTGGAGATAAAGTGACTTCAGACCAAAAGGAAACCCCTTTTATTATTGATCAAACACACCCAACCTATTATTTAGGTGTGAAGGAATTTTACGTGGGAACCGCTCTCGTCAAAACAGAATTTCAAGCCCTTTTTGACACTGGCACCTCATTTACATACTTGGTTGATTCAGTTTACAAAAGCCTTACATCAAATTATCATCTGCAGAGGAAAGCCTCTCCATTGGCAATTGATGATTCAAACATTCCTTTCGAGTTTTGTTATGAAATCAGCAACAGTCAAAGCATTAATCAAGGGCCTAATATATCTTTCAACTTCAATGGAGGAAACAACTTTTCTGCTATTCAACCATTAGTCCTTATTGGTGATGAGAATGGGAAGTTGGTAGGTTACTGTTTGGCAGTGCTTCAGAGCTCGACAGTCACAATTATTGGTCAGAATTTCATGGCAGGTTATCAACTTGTCTTTGACCGGGAGCAGCTCAAACTGGGGTGGAAGGAAGCTAACTGTAATGATTTGGAGTATAGCAGCAGTGCAGCGTCAGCACCATCAAGTTTGACACCACTTCAGTCACCCCTTGCCCCTTCCTCGGTCGATGACAGCGAACACAAATCTAATACAACTACAAATAGTTCAACAGGGTCACCTCCGAAGCAGTCCATATCTCCTGATGCTCTACCAGCTAATCCTAATGCCATCGGGAATGATGCAACCCATTTAAGCTTATTTTTTCTGTGCAATGTCATCTCATTTACAGTCACCATCACCATTCTTACAAATCTCTTTTAAATCATTTATGCACAATGAAGATGTCTATGGTGACATGAGTTTTTCCTTCCCATTATTTTTATTGTTCCAATTTTTATAAAGTCACCATAGAAGTTCCATATTTAATCATCTTTACAGCTGTAAAGATGTACAGTTTTAATCATGAAAGAAGTAATAGGCCGTTCATGTACAGCAGAAGTTCCATTGTTGTAACTATGATTAGCATCAAACTTCAGTTTTTGTTTTAACTTTAATTAGGAATAGTGGAGAAGGATTTTTGATGTATGTGGTGATATATGTGTCCTATATGAATGAATTTGATTTGAGATTGTGGGCATTTAAATTTCG

Fln Alignment:

Contig6133___MENSTLGFICHILFFIALGLRYSNGQSYSLQMYHKFSPEVKEWMTWRHGLDTDGWPTEGRNEYYKALYHHDSARHDRKLxxxxSLTFLAGNMTNSFSQLGYLFYTMVQVGTPNVTLLVALDTGSDLFWVPCDCQECAPTSAPYYGL
D5A9P2______________MESPTVSFIYSLILFTSLGFQNSNGQSYSLQMYHKFSNEVKEWMTWRHGLDTDGWPVEGSNEYYKALYHHDSARHGRKLxxxxSLTFLEGNETVEIPQLGFLFYSMVQVGTPNVTLFVALDTGSDVFWVPCDCQACAPTSAASYGL

SNPs (tot: 65)

Position	A %	C %	G %	T %	Probability
310			20	80	0.995493
312		20		80	0.995493
316	20		80		0.995493
318		20		80	0.995493
321	80		20		0.995493
322			20	60	0.99255
323		20		80	0.995493
332	80	20			0.995493
333	20	80			0.995493
381	75	25			0.996473
385			25	75	0.996473
388	25	75			0.996473
389	75	25			0.996473
390		25	75		0.996473
391	75			25	0.996473
394			25	75	0.996473
395	75	25			0.996473
398	75		25		0.996473
400	75	25			0.996473
402	75	25			0.996473
405	25			75	0.996473
550		50	33		1.0
567	85	14			0.993533
569		85		14	0.993533
570	85	14			0.993533
572			85	14	0.993533
573		85	14		0.993533
575	85	14			0.993533
577	85			14	0.993533
580	85		14		0.993533
582	85			14	0.993533
583	85	14			0.993533
712		14	85		0.993533
713	14	85			0.993533
777	85		14		0.993533
819			57	42	1.0
874	10		90		0.90298
1024		16	83		0.994512
1029	83		16		0.994512
1030			83	16	0.994512
1031		83	16		0.994512
1043		75	25		0.996473
1049		25		75	0.996473
1050	25		75		0.996473
1061		28		71	1.0
1064	57	14	28		1.0
1068	14			85	0.993533
1071		71	28		1.0
1072			28	71	1.0
1078	50	50			1.0
1234	11			88	0.923564
1237			10	90	0.90298
1528		11	88		0.999952
1531		11	88		0.999952
1532	87		12		0.999964
1536		12	87		0.999964
1537	87	12			0.999964
1538	81			12	0.999943
1543	86			13	0.999973
1544	86	13			0.999973
1545	13		86		0.999973
1546		13		86	0.999973
1548		13	86		0.999973
1549	13		86		0.999973
1597		5	82		0.999919

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain