SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene6114

Length: 1428 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco3 v1 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene6114

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Glutamine synthetase cytosolic isozyme n=2 Tax=Pinaceae RepID=GLNA_PINSY	-	-	85%
FL-Next	sp=Glutamine synthetase cytosolic isozyme; Pinus sylvestris (Scots pine).	-	-	96%
Blast2go	protein	-	-	89%

Source	ECs	Term	Type	e value	Identity
Blast2go	Glutamate--ammonia ligase.	EC:6.3.1.2	-	0.0	89%

Source	KEGGs	Term	Identity
Blast2go	Alanine, aspartate and glutamate metabolism	00250	89%
Blast2go	Arginine and proline metabolism	00330	89%
Blast2go	Nitrogen metabolism	00910	89%
Blast2go	Metabolic pathways	01100	89%

Source	GOs	Term	Type	Identity
Blast2go	glutamine biosynthetic process	GO:0006542	Biological Process	89%
Blast2go	glutamate-ammonia ligase activity	GO:0004356	Molecular Function	89%
Blast2go	ATP binding	GO:0005524	Molecular Function	89%
Blast2go	cytoplasm	GO:0005737	Cellular Component	89%

Source	InterPros	Term	Type	Identity
Sma3	Glutamine synthetase, catalytic domain	IPR008146	-	-
Sma3	Glutamine synthetase, beta-Grasp	IPR008147	-	-
Sma3	Glutamine synthetase/guanido kinase, catalytic domain	IPR014746	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: P52783

Fln msg: Overlapping hits, possible frame ERROR between 802 and 781, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 287 - 357

Fln protein: GSPQAIFRDPFRRGKHILVICDAYSPNGTAIPSNKRAAAAKIFNEKAVIDEETWYGLEQEYTLLQKDVKWPLGWPIGGYPGPQGPYYCGVGADKAWGRDMVDAHYKACLYSGINISGINGEVMPGQWEFQVGPSVGISAADELWCARFIMERITEKAGVVLSFDPKPIEGDWNGAGCxxxxxxxxxMRKEGGFEVIKKAIEKLKLRHKEHISAYGEGNERRLTGRHETADMNTFSWGVANRGASVRVGRDTEKEGKGYFEDRRPASNMDPYIVTSMIAETTILWKP*

Protein Length: 288

Fln nts: GGTTTTACTGTTCTCTTCCTTTCATCATCGTGCATAGGCCTGGGTATATGGTCCTTTTGTTTTATTAATTTGATTCTGTACGGCCCAAAATGCAACATAAATTAACAGTTTAGAATATTCTTCCCTGTACTCTGAAATTTCTAAGATATTACTTGATTCCAAGTCTATATGTGTTTCCAATATTAAATGTTCTTGTTCTCATAGTTTGGAACATATATAACCCAATTACGTGGTTTGTTCTGATTGAGGCAGTCCACAAGCTATCTTCCGTGATCCATTTCGCCGAGGAAAGCACATTTTGGTAATCTGTGATGCCTACTCTCCTAATGGGACTGCTATTCCTTCCAACAAGAGGGCTGCAGCAGCGAAAATTTTTAACGAAAAGGCGGTTATTGATGAAGAGACATGGTACGGGCTTGAACAAGAATATACACTGTTGCAAAAGGACGTCAAATGGCCTCTTGGCTGGCCAATTGGTGGCTACCCCGGTCCTCAGGGCCCATATTACTGTGGAGTTGGAGCTGACAAAGCCTGGGGACGTGACATGGTTGATGCCCATTATAAGGCTTGTCTCTATTCAGGAATCAATATCAGTGGCATCAATGGAGAAGTCATGCCAGGGCAGTGGGAATTTCAAGTAGGTCCGTCAGTGGGTATCTCAGCAGCAGATGAGCTGTGGTGTGCTCGTTTTATTATGGAGAGGATTACAGAAAAGGCGGGTGTCGTTCTGTCCTTTGATCCCAAGCCAATTGAGGGGGACTGGAATGGTGCTGGATGCxxxxxxxxxxxxxxxxxxxxxxxxxxxATGCGCAAGGAGGGAGGCTTCGAAGTAATTAAGAAAGCAATAGAAAAACTGAAGTTGAGGCATAAGGAGCATATTTCTGCCTATGGAGAGGGCAATGAGAGACGCCTCACTGGTCGGCACGAGACAGCAGACATGAATACCTTTTCCTGGGGTGTTGCAAATCGAGGAGCTTCAGTTAGAGTGGGCCGGGACACAGAAAAAGAAGGAAAAGGTTATTTTGAGGACCGTCGACCTGCTTCAAACATGGATCCATACATAGTGACTTCTATGATTGCTGAGACGACCATTCTATGGAAACCTTAAATTACAAAGTGGAGGCCAGTTACACGCGTGGTCGTGGTGCTTTGCTTTGGAGGCCAGCGTCACTGATAAGCTATATGTATGTAAATGTGATGCCAATGTTTAAGTAGGGTGGTAACTTTGCTTTGGTTGTGGGTAGACCTGAACTTTGGTCAAACAATTTCCTCTTTGTATATGGATATATATATATATGTATTTGTTTTACTTGTAATATGGCGAGGGCTTTAAAAGACTCTCTTTTACCTTTGTTTATTTCGTTGTCGAAGATGTATTCAACAAATTGTTTAGAGTGTTTGAATATGATGATATTTTCTTTGTATGGAA

Fln Alignment:

Contig6114___PQAIFRDPFRRGKHILVICDAYSPNGTAIPSNKRAAAAKIFNEKAVIDEETWYGLEQEYTLLQKDVKWPLXXXXXXXXXXXXXXXXXXXADKAWGRDMVDAHYKACLYSGINISGINGEVMPGQWEFQVGPSVGISAADELWCARFIMERITEKAGVVLSFDPKPIEGDWNGAGCxxxxxxxxMRKEGGFEVIKKAIEKLKLRHKEHISAYGEGNERRLTGRHETADMNTFSWGVANRGASVRVGRDTEKEGKGYFEDRRPASNMDPYIVTSMIAETTILWKP
P52783______________PQAIFRDPFRRGKHILVICDAYSPNGTAIPSNKRAAAAKIFNEKAVSDEETWYGLEQEYTLLQKDVKWPLGWPIGGYPGPQGPYYCGVGADKAWGRDIVDAHYKACLYSGINISGINGEVMPGQWEFQVGPSVGISAADELWCARFIMERITEKAGVVLSFDPKPIEGDWNGAGCxxxxxxxxMRKEGGFEVIKKAIEKLKLRHKEHISAYGEGNERRLTGRHETADMNTFSWGVANRGASVRVGRDTEKEGKGYFEDRRPASNMDPYIVTSMIAETTILWKP

SNPs (tot: 13)

Position	A %	C %	G %	T %	Probability
2			66	33	0.997452
107	83		16		0.994512
254	16		83		0.994512
780	20	60			0.99255
781	20	60			0.99255
782	80		20		0.995493
791	83			16	0.994512
830	88		11		0.923564
1239	4		95		0.997494
1289		13	21	65	1.0
1298	75		25		1.0
1359	66		33		1.0
1427		50		50	1.0

SSRs (tot: 1)

Start position	End position	Sequence	Length
1283	1296	AT AT AT AT AT AT AT	14

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain