SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene5646

Length: 1675 nt

Origin of reads: Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene5646 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Asparagine synthetase n=3 Tax=Pinaceae RepID=E7EAQ2_PINPS	-	-	86%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	92%
Blast2go	asparagine synthetase	-	-	90%

Source	ECs	Term	Type	e value	Identity
Blast2go	Asparagine synthase (glutamine-hydrolyzing).	EC:6.3.5.4	-	0.0	90%

Source	KEGGs	Term	Type	Identity
Blast2go	Alanine, aspartate and glutamate metabolism	00250		90%
Blast2go	Nitrogen metabolism	00910		90%
Blast2go	Metabolic pathways	01100		90%
Blast2go	Biosynthesis of secondary metabolites	01110		90%
Blast2go	Aspartate--ammonia ligase.	EC:6.3.1.1	-	90%

Source	KEGGs	Term	Identity
Blast2go	Alanine, aspartate and glutamate metabolism	00250	90%
Blast2go	Cyanoamino acid metabolism	00460	90%
Blast2go	Nitrogen metabolism	00910	90%
Blast2go	Metabolic pathways	01100	90%
Blast2go	Biosynthesis of secondary metabolites	01110	90%

Source	GOs	Term	Type	Identity
Blast2go	hyperosmotic salinity response	GO:0042538	Biological Process	90%
Blast2go	asparagine biosynthetic process	GO:0006529	Biological Process	90%
Blast2go	glutamine metabolic process	GO:0006541	Biological Process	90%
Blast2go	asparagine synthase (glutamine-hydrolyzing) activity	GO:0004066	Molecular Function	90%
Blast2go	ATP binding	GO:0005524	Molecular Function	90%
Blast2go	aspartate-ammonia ligase activity	GO:0004071	Molecular Function	90%

Source	InterPros	Term	Type	Identity
Sma3	Glutamine amidotransferase, class-II	IPR000583	-	-
Sma3	Asparagine synthase	IPR001962	-	-
Sma3	Asparagine synthase, glutamine-hydrolyzing	IPR006426	-	-
Sma3	Rossmann-like alpha/beta/alpha sandwich fold	IPR014729	-	-
Sma3	Glutamine amidotransferase, type II	IPR017932	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LMV0

Fln msg: your sequence is shorter than subject: 447 - 591

Fln protein: VVTLAVTDGSVWFASEMKALHDDCERFELFPPGHIYSSKQGGLRRWYSPPWYSENVIPSTPYDPSVLRRAFEKAVTKRLMTDVPFGVLLSGGLDSSLVAAVAARHLAATNAAKQWGDKLHSFCVGLQGSPDLKAAKEVAEYLGTVHHEFHFTVQDGIDAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPNKEEFHQETCRKIKALHMYDCLRANKSTSAWGVEARVPFLDKEFVNVAMAIDPEWKMVKPDIGRIEKWVLRRAFDDEQNPYLPKHILYRQKEQFSDGVGYSWIDGLKSHAAEHVTDTMLGNAKYIFPHNTPTTKEAYYYRMIFERFYPQNAARLTVPGGPSVACSTAKAVEWDAGWAHNLDPSGRAALGVHASAYQESKNSVTANGNVDMLRKISPKMETVGAPQVVINC*

Protein Length: 448

Fln nts: CGTCGTGACCTTGGCTGTCACTGATGGATCTGTTTGGTTTGCGTCTGAAATGAAAGCTTTGCATGATGATTGTGAACGTTTTGAGTTGTTTCCTCCTGGACATATATACTCCAGCAAACAAGGAGGTCTGCGCAGATGGTACAGCCCACCTTGGTATTCAGAAAATGTTATACCGTCCACTCCATATGACCCTTCCGTTCTAAGACGAGCATTTGAGAAAGCAGTCACAAAGAGGCTGATGACGGATGTTCCATTTGGAGTACTGTTATCAGGAGGGCTTGACTCATCCTTAGTTGCGGCTGTTGCGGCTCGCCATTTGGCTGCTACCAACGCTGCAAAACAGTGGGGCGACAAACTGCACTCTTTCTGTGTTGGATTACAGGGTTCTCCAGATTTGAAGGCTGCAAAAGAGGTAGCAGAGTACCTTGGTACTGTTCATCACGAATTCCATTTCACTGTGCAGGATGGTATAGATGCAATCGAGGACGTTATATACCATGTTGAAACATATGACGTGACAACTATCAGAGCGAGCACGCCCATGTTTTTAATGTCTCGGAAAATCAAATCGCTAGGAGTGAAGATGGTTCTGTCTGGAGAAGGATCAGATGAGATATTTGGTGGGTATTTGTACTTCCACAAGGCTCCTAACAAAGAGGAATTCCACCAGGAAACATGTCGTAAGATAAAGGCCCTTCACATGTATGATTGCTTGAGGGCTAATAAGTCGACATCTGCATGGGGTGTGGAGGCCCGTGTTCCATTTTTAGATAAGGAATTCGTCAATGTTGCCATGGCCATTGATCCCGAGTGGAAGATGGTGAAGCCTGACATTGGGCGCATTGAGAAGTGGGTCTTGCGGAGGGCATTCGATGACGAGCAGAATCCTTATCTTCCCAAGCACATTCTGTACAGACAGAAGGAGCAATTCAGCGATGGGGTTGGGTACAGTTGGATTGACGGGTTAAAATCACATGCGGCAGAGCATGTGACAGATACGATGCTGGGCAATGCCAAATATATATTCCCCCATAATACTCCCACTACAAAGGAGGCTTACTACTACAGGATGATATTTGAAAGATTCTATCCTCAGAACGCGGCACGGTTGACGGTTCCGGGCGGGCCGAGCGTGGCTTGCAGCACCGCAAAGGCCGTGGAATGGGATGCTGGGTGGGCTCACAATCTGGATCCCTCTGGCAGGGCCGCTCTAGGCGTCCATGCATCGGCGTACCAAGAATCTAAAAATAGCGTCACCGCGAATGGGAATGTGGACATGTTGCGCAAAATATCGCCAAAAATGGAGACAGTCGGCGCTCCACAAGTCGTTATCAACTGTTAG___GTGTTGACGGAGAGAAGCTGTCGCTGGGAATCGCTGACGAGTCTGGCTGTCTTTATCACTACACTGGTTGAGCTGTCTGTCTAGTTAACCCATCCACACAAATGGGCGGGCCGACTAAATGTTTGTTGACCATCCGTCCTATTTCACTACGAGTAAGCGACGGCTTGTATGTATGCAATGGCTGTTTGGGATACGCGTTTTCCATTTGCTAGCTTTTATGTATCAAGATGTCGCGTGATCACGTTTTTACGTTCATGGTTGTTTAAATGTTTTGTCGGATAAATATCATGATATTGTAAATTTTTGTAAGTTCTTTTGGTTTTAACTAAAGAA

Fln Alignment:

Contig5646___DGSVWFASEMKALHDDCERFELFPPGHIYSSKQGGLRRWYSPPWYSENVIPSTPYDPSVLRRAFEKAVTKRLMTDVPFXXXXXXXXXXXXVXXXXXXXXXXXXXAKQWGDKLHSFCVGLQGSPDLKAAKEVAEYLGTVHHEFHFTVQDGIDAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPNKEEFHQETCRKIKALHMYDCLRANKSTSAWGVEARVPFLDKEFVNVAMAIDPEWKMVKPDIGRIEKWVLRRAFDDEQNPYLPKHILYRQKEQFSDGVGYSWIDGLKSHAAEHVTDTMLGNAKYIFPHNTPTTKEAYYYRMIFERFYPQNAARLTVPGGPSVACSTAKAVEWDAGWAHNLDPSGRAALGVHASAYQESKNSVTANGNVDMLRKISPKM-ETVGAPQVVI
B8LMV0______________DGSVWFASEMKALHDDCERFESFPPGHIYSSKQGGLRRWYNPPWYSESLIPSTPYDPLVLRRAFEKAVIKRLMTDVPFGVLLSGGLDSSLVAAVAARHLATTNAAKQWGEQLHSFCVGLQGSPDLKAAKEVAEYLGTFHHEFHFTVQDGIDAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPNREEFHQETCRKIKALHMYDCLRANKSTSAWGVEARVPFLDKEFINIAMDIDPDWKMVKPDIGRIEKWVLRKAFDEEQNPYLPKHILYRQKEQFSDGVGYSWIDGLKSHAAEHVTDTMLGNAKYIFPHNTPTTKEAYYYRTIFERLYPQNAARLTVPGGPSVACSSAKAVEWDDGWAGNLDPSGRAALGVHASAYQESKNSVAANGSVDMLGKLPPTMKENVGARQIVI

SNPs (tot: 27)

Position	A %	C %	G %	T %	Probability
317		66		33	0.997452
328		66		33	0.997452
338	66	33			0.997452
352		66	33		0.997452
368	33			66	0.997452
416		60	40		1.0
421		20		80	0.995493
448		40		60	1.0
465	20		80		0.995493
561		60		40	1.0
597	25			75	0.996473
599	25			75	0.996473
1068	14	85			0.993533
1073		85		14	0.993533
1142	33		66		0.997452
1144		66		33	0.997452
1146	33			66	0.997452
1149	33			66	0.997452
1169	33			66	0.997452
1171	66		33		0.997452
1358	42			57	1.0
1451		33		66	1.0
1487	16		83		0.994512
1488		16	83		0.994512
1490	83			16	0.994512
1491			83	16	0.994512
1589	66		33		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain