SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene5294

Length: 1591 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene5294 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	poly(A) polymerase 1 [Arabidopsis thaliana] gb\|AAF97277.1\|AC034106_20 Strong similarity to nuclear poly(A) polymerase from Arabidopsis thaliana gb\|AF255297 and contains a Nucleotidyltransferase PF\|01909 domain. ESTs gb\|AV536592, gb\|AV553256, gb\|AV561630,	-	-	77%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	97%
Blast2go	poly polymerase	-	-	87%

Source	ECs	Term	Type	e value	Identity
Blast2go	Polynucleotide adenylyltransferase.	EC:2.7.7.19	-	0.0	87%

Source	GOs	Term	Type	Identity
Blast2go	RNA polyadenylation	GO:0043631	Biological Process	87%
Blast2go	sugar binding	GO:0005529	Molecular Function	87%
Blast2go	RNA binding	GO:0003723	Molecular Function	87%
Blast2go	protein binding	GO:0005515	Molecular Function	87%
Blast2go	polynucleotide adenylyltransferase activity	GO:0004652	Molecular Function	87%
Blast2go	nucleus	GO:0005634	Cellular Component	87%

Source	InterPros	Term	Type	Identity
Sma3	Nucleotidyl transferase domain	IPR002934	-	-
Sma3	Sugar transporter, conserved site	IPR005829	-	-
Sma3	Protein of unknown function DUF599	IPR006747	-	-
Sma3	Poly(A) polymerase, RNA-binding domain	IPR007010	-	-
Sma3	Poly(A) polymerase, central domain	IPR007012	-	-
Sma3	Inorganic pyrophosphatase	IPR008162	-	-
Sma3	TonB box, conserved site	IPR010916	-	-
Sma3	Poly(A) polymerase	IPR014492	-	-

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LRY2

Fln msg: Distance to subject end: 215 aas, your sequence is shorter than subject: 497 - 712

Fln protein: MGSPGQTVTQSNGQYLGVTEPISLGGPSEFDYTKTAELEKFLVSAGLYESQEEAVRREEVLGRLDQIVKVWVRKISRSKGFSEQVVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREEDFFIELYEMLAEMPEVTELHPVPDAHVPIMNFKFNGISIDLLYARLSLWVIPEDLDISQDSILRNADEQTVRSLNGCRVTDQILRLVPNIQNFRTTLRCMKLWAKRRGVYSNVAGFLGGVNWALLVARICQLYPNALPSMLVCRFFRVYTQWRWPNPVMLCAIEEGPLGLPVWDPRKNPRDRLHQMPIITPAYPCMNSSYNVSSSTLRVMTEEFQRSNQICEELELNKVGWNILFEPYPFFEAYKNYLEIDISAEGADDLRKWKGWIESRLRLLTLKIERQTFGMLQCHPHPDDFSDPQRPYHCCFFMGLQRKQGMPIQEGEQFDIRVTVDEFRHSVKMYTLWKPGMEINVSHIKRRSIPLFVFPGGVRPPRT

Protein Length: 498

Fln nts: ATGGGGAGAATATTTCTCCATCCCGTGGTTGCTTTTATAGTTAATACATTGTGAAAAAAAAACTTCTATAAAATATCGTTGATAATTGCTAGTGAGCTTG_-_ATGGGGAGCCCCGGGCAAACAGTGACACAGAGTAATGGTCAATATTTGGGAGTGACGGAACCGATATCTCTCGGAGGTCCTTCGGAGTTCGATTATACCAAAACTGCTGAGCTCGAGAAGTTTCTTGTGAGTGCTGGTTTATATGAAAGTCAAGAAGAAGCTGTGCGCAGAGAAGAAGTGCTCGGGAGACTGGATCAGATTGTTAAGGTATGGGTTAGAAAGATAAGCAGAAGCAAGGGCTTCAGTGAGCAGGTTGTCCAGGAAGCCAATGCTAAGATTTTTACGTTTGGGTCTTACCGGCTTGGCGTTCATGGACCTGGTGCGGACATTGATACACTTTGTGTTGGGCCTCGGCATGCAACTCGAGAGGAGGATTTTTTTATTGAGCTTTATGAAATGCTAGCAGAAATGCCAGAAGTAACAGAGCTGCATCCTGTACCAGATGCACATGTCCCAATCATGAATTTTAAGTTCAATGGAATATCGATAGATCTACTTTATGCAAGACTTTCTCTTTGGGTGATTCCTGAAGATCTAGACATCTCCCAGGATTCTATACTCCGTAATGCTGATGAGCAGACTGTTCGAAGTCTTAATGGATGTAGAGTAACTGATCAGATTTTACGTTTGGTACCAAATATTCAGAATTTTCGAACCACACTTAGGTGTATGAAGCTTTGGGCAAAACGACGTGGTGTCTATTCAAATGTAGCAGGTTTTCTAGGGGGTGTTAATTGGGCATTACTTGTGGCCAGAATATGTCAGCTCTACCCAAATGCATTGCCTAGCATGTTGGTTTGCCGCTTTTTCCGAGTATATACCCAGTGGCGCTGGCCAAACCCTGTCATGCTTTGTGCTATTGAAGAGGGTCCACTAGGCCTTCCTGTTTGGGATCCACGGAAAAATCCTCGTGACAGGCTTCATCAGATGCCTATAATTACCCCAGCATATCCCTGCATGAATTCAAGTTATAATGTGTCATCAAGTACTCTACGTGTTATGACGGAAGAATTTCAGAGAAGCAATCAGATTTGTGAGGAACTGGAGTTGAACAAAGTTGGTTGGAATATTCTCTTTGAGCCTTATCCGTTCTTCGAAGCTTACAAAAATTATTTAGAAATAGACATATCTGCAGAAGGAGCAGATGATCTACGGAAATGGAAAGGCTGGATTGAATCTCGTCTTCGTTTACTCACTTTAAAAATTGAAAGACAAACATTTGGCATGCTTCAGTGTCATCCACACCCGGATGACTTTTCAGATCCGCAAAGGCCATATCATTGTTGTTTCTTTATGGGCTTGCAGCGTAAACAAGGTATGCCCATCCAAGAAGGAGAACAGTTTGACATAAGGGTAACTGTGGATGAATTCAGGCATTCTGTCAAAATGTACACACTGTGGAAACCGGGCATGGAGATTAATGTTTCTCATATTAAGCGGAGGAGTATTCCCTTATTTGTTTTCCCAGGTGGTGTCCGCCCTCCACGGACA

Fln Alignment:

Contig5294___MGSPGQTVTQSNGQYLGVTEPISLGGPSEFDYTKTAELEKFLVSAGLYESQEEAVRREEVLGRLDQIVKVWVRKISRSKGFSEQVVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREEDFFIELYEMLAEMPEVTELHPVPDAHVPIMNFKFNGISIDLLYARLSLWVIPEDLDISQDSILRNADEQTVRSLNGCRVTDQILRLVPNIQNFRTTLRCMKLWAKRRGVYSNVAGFLGGVNWALLVARICQLYPNALPSMLVCRFFRVYTQWRWPNPVMLCAIEEGPLGLPVWDPRKNPRDRLHQMPIITPAYPCMNSSYNVSSSTLRVMTEEFQRSNQICEELELNKVGWNILFEPYPFFEAYKNYLEIDISAEGADDLRKWKGWIESRLRLLTLKIERQTFGMLQCHPHPDDFSDPQRPYHCCFFMGLQRKQGMPIQEGEQFDIRVTVDEFRHSVKMYTLWKPGMEINVSHIKRRSIPLFVFPGGVRPPRT
B8LRY2______________MGSPGQTATQSNGQYLGVTEPISLGGPSEFDYIKTAELEKFLVSAGLYESQEEAVRREEVLGRLDQIVKVWVRKISRNKGFGEQVVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREEDFFIELYEMLADMPEVTELHPVPDAHVPVMKFKFNGISIDLLYAKLSLWVIPEDLDISQDSILRNADEQTVRSLNGCRVTDQILRLVPNIQNFRTTLRCMKLWAKRRGVYSNVAGFLGGVNWALLVARICQLYPNALPSMLVCRFFRVYTQWRWPNPVMLCAIEEGPLGLPVWDPRKNPRDRLHQMPIITPAYPCMNSSYNVSSSTLRVMTEEFQRSNQICEELELNKVCWNILFETYPFFEAYKNYLEIDISAEGADDLRKWKGWTESRLRLLTLKIERQTFGMLQCHPHPDDFSDPQRPYHCCFFMGLQRKQGMPIQEGEQFDIRVTVDEFRHSVKMYTLWKQGMEINVSHIRRRNIPLFVFPGGVRPPRT

SNPs (tot: 27)

Position	A %	C %	G %	T %	Probability
409		20		80	0.995493
412			83	16	0.994512
549		11		88	0.923564
576	12	87			0.992554
578	12			87	0.992554
579			87	12	0.992554
585	85		14		0.993533
757	16	83			0.994512
758	83	16			0.994512
792	83		16		0.994512
802		83	16		0.994512
806	16		83		0.994512
807		16		83	0.994512
808			16	83	0.994512
809	16			83	0.994512
810		16		83	0.994512
1068		62		37	1.0
1071		12		87	0.992554
1072		12	87		0.992554
1073	12			87	0.992554
1128		16		83	0.994512
1134			50	50	1.0
1135	50		50		1.0
1142	50		50		1.0
1148	50	50			1.0
1150	50		50		1.0
1153	50		50		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain