SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene5209

Length: 2885 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene5209
CGTCGTGACCTTGGCTGTCACTCAATTTTGTTCCCTAGTGTGGGCATATTAAGTAGAGTTTGTCATTACAGACGTGTTTGTATGGGATTGGCTTTATTAAATTAAAGCTCTCACTAAATAAATCAGGAAATGCCAGGGAAGCCAGCAGGCTGAATCATTGGGCGCCCTATATATAAAGGCTGTGGTTTGCATCAATATTTGGGCCATAGGCATTGTTAGTGGGCTAACTCTGAGGTCACACAAAACCTTGAAGTCTTGTTTCTGTGGACCTCTGGATTGCTTTGCATCTGTCGAGGCTTCTGCTGTCAGTCCAAGATCTAGGTCTATTTGCATCCAATTTTAGAACGGGGTATTTTCTTTTATTCTTGATTGGCTGTTATTATTCAAACTGCGATTTGTTGGAAGAATGGAGAATGGCAGTGAAGGGTATGTGCCCAAGAACATTCTTATCACGGGTGCAGCAGGATTCATTGCCTCTCATGTAGCCAACAGGCTGGTGAGAAAATACCCACAATACAAGATTGTGGTATTGGACAAGCTGGATTATTGTTCGAATCTGAAGAATCTCCTGCCCTCCAGGTCTTCTCCCAATTTCAAATTTGTGAAGGGAGATATTGGGAGTGCAGATCTGGTAAATTATCTGCTGATCACAGAACAGATTGATACTATAATGCATTTTGCGGCACAAACCCATGTGGACAATTCATTTGGGAACAGCTTTGAGTTCACCAAGAACAACATCTATGGTACTCATGTCCTTCTTGAAGCATGCAAGGTGACTGGATTGATCAGAAGATTCATTCATGTGAGCACTGATGAAGTTTATGGTGAAACAGAGAAGGATGCCATTGAGGGAAATCATGAGGCCAGTCAGCTTTTACCCACTAATCCTTATTCTGCAACCAAGGCAGGAGCAGAGATGCTGGTCATGGCTTATGGGAGATCCTACGATTTGCCTGTGATCACCACAAGAGGTAACAATGTATATGGACCTAATCAGTTTCCTGAAAAACTGATACCCAAGTTCATTCTTCTGGCGATGCAAGGGAAGCCACTCCCAATCCATGGCGACGGATCCAATGTTAGGAGCTATTTGTACTGCGAGGACGTTGCTGAGGCTTTTGAAGTCGTTTTGCATAAAGGTGAAGTGGGGCATGTGTATAATATTGGTACACAAAAGGAGAGAAGGGTCATTGATGTGGCCAAGGATATCTGTAGGCTCTTTTCATTAGATGCAGAGAAGGTTATAAAGTTTGTAGACAACAGGCCTTTCAATGATCAGAGATATTATCTGGATGATAAGAAACTCAAGGATCTGGGATGGTCTGAGAGGACTAAATGGGAAGAGGGCTTGAAGAAAACAATGGATTGGTACATTAGACAACCTGATTGGTGGGGTGATGTGTCCGGTGCTCTGCTACCTCATCCAAGGATGCTCACAATGGCAGGAGTTGAGAGGTATACAGAAGCCCATGATGTGGTTGACACAACATCTCCCTCGCCAGAGAATGGCATCGAGCAGAATCACATGCTAGAGCGCTCCAACGGCCTTATTCCAAAGTCAACCTTGAAGTTCTTGATCTATGGGCGCAATGGCTGGATCGGAGGGCTTCTTGGGCAGATATGCCAAAAGCAGGGAATTACATATGAATATGGAAATGGGAGATTAGAGAATAGGTCTCAGATCCTAATGGACATACAGAACATCAAGCCTACTCATGTGTTTAATGCTGCAGGTGTGACAGGTCGGCCTAACGTGGATTGGTGTGAATCCCACAAGCCCGAGACTATTACAACCAATGTGGCGGGAACACTGAATTTGGCTGATATTTGCAAGGAGCAGGGTCTTTTGCTTGTAAATTTTGCAACTGGCTGTATTTTTGAATATGACGATGCTCATCTTGAAGGCAGTGGTATTGGATTTAAGGAGGAAGACAAACCCAACTTCATTGGGTCCTTTTATTCGAAGACCAAGGCCATGGTTGAAGATCTCCTACAAAATTTTGAAAATGTCTGTACTCTTAGGGTGAGAATGCCCATCTCCTCGGACCTGAGTAACCCTCGCAATTTCATCACAAAGATCACTCGATATGAGAAAGTGGTAAATATACCAAACAGTATGACAGTCCTTGATGAGTTGCTGCCAATCTCGATTGAGATGGCAAAAAGAAATTGCACTGGCATATGGAACTTCACTAACCCAGGTGTTGTGAGCCATAATGAAATCTTGCAGATGTACAGAGAATATATTGATCCCACTTTCAAGTGGACAAACTTTACTCTTGAAGAACAGGCAAAAGTCATAGTTGCTCCTCGAAGTAATAATGAACTGAATGCCACCAAGTTGAAGACGGAATTTCCACAGTTGCTGTCAGTAAAAGATTCATTATTGAAGTATGTCTTTGAGCCCAACAGGAAGAACAAGTTAGCCAATGGAGTTTAGTCCTTGCTTAAGATCAAATTTTCATTAGGTGTAGTAAAGTTTTTCTCTGCTTAGGCAGGCAAAACATCCATATGCTTAATTGGGGGACAATTCAAAAGGTCGTCCAGTACTTTGTTATTTTTAGCTCACAAATGGAGGAGAGCTTATTTGGTAGGATATTCTAGATATGTTCTACTATTTTTCTTCTGATTTTACATTTTAGTTCACCAACGGAGGAGCCGACTAGCTAGGGTATTCTAGATAGGTTCTACAATTCTTGTTCTGTTTTTATATTTACATTCTTTCTATGAATGTAAGTCAACTTACAAGGTTAATGCCATTGAATTTCCGCTTCCAAGGAATTCTACTATTTGGAATATATTCGTCTTTGACAAGACTGTTCTTCTTGTATGAAAAAAGTTCTCACATTTTTATAAAATAACTGCTCAAGCTTTGTGTAGTT

Ace file of the UniGene sp_v2.0_unigene5209 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	UDP-glucose 4,6-dehydratase [Arabidopsis thaliana] sp\|Q9SYM5.1\|RHM1_ARATH RecName: Full=Probable rhamnose biosynthetic enzyme 1 gb\|AAD30579.1\|AC007260_10 Similar to dTDP-D-glucose 4,6-dehydratase [Arabidopsis thaliana] gb\|AAK68773.1\| Similar to dTDP-D-glu	-	-	81%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	80%
Blast2go	probable rhamnose biosynthetic enzyme 1-like	-	-	89%

Source	ECs	Term	Type	e value	Identity
Blast2go	dTDP-4-dehydrorhamnose reductase.	EC:1.1.1.133	-	0.0	89%

Source	KEGGs	Term	Type	Identity
Blast2go	Polyketide sugar unit biosynthesis	00523		89%
Blast2go	Metabolic pathways	01100		89%
Blast2go	Biosynthesis of secondary metabolites	01110		89%
Blast2go	UDP-glucose 4,6-dehydratase.	EC:4.2.1.76	-	89%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Amino sugar and nucleotide sugar metabolism	00520		0.0	89%
Blast2go	dTDP-glucose 4,6-dehydratase.	EC:4.2.1.46	-	0.0	89%

Source	KEGGs	Term	Identity
Blast2go	Polyketide sugar unit biosynthesis	00523	89%
Blast2go	Metabolic pathways	01100	89%
Blast2go	Biosynthesis of secondary metabolites	01110	89%

Source	GOs	Term	Type	Identity
Blast2go	extracellular polysaccharide biosynthetic process	GO:0045226	Biological Process	89%
Blast2go	UDP-rhamnose biosynthetic process	GO:0010253	Biological Process	89%
Blast2go	flavonol biosynthetic process	GO:0051555	Biological Process	89%
Blast2go	auxin efflux	GO:0010315	Biological Process	89%
Blast2go	dTDP-4-dehydrorhamnose reductase activity	GO:0008831	Molecular Function	89%
Blast2go	UDP-glucose 4,6-dehydratase activity	GO:0050377	Molecular Function	89%
Blast2go	UDP-L-rhamnose synthase activity	GO:0010280	Molecular Function	89%
Blast2go	coenzyme binding	GO:0050662	Molecular Function	89%
Blast2go	dTDP-glucose 4,6-dehydratase activity	GO:0008460	Molecular Function	89%
Blast2go	soluble fraction	GO:0005625	Cellular Component	89%

Source	InterPros	Term	Type	Identity
Sma3	Aldo/keto reductase, conserved site	IPR018170	-	-
Sma3	NAD-dependent epimerase/dehydratase	IPR001509	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NN20

Fln msg: your sequence is longer than subject: 679 - 300

Fln protein: MENGSEGYVPKNILITGAAGFIASHVANRLVRKYPQYKIVVLDKLDYCSNLKNLLPSRSSPNFKFVKGDIGSADLVNYLLITEQIDTIMHFAAQTHVDNSFGNSFEFTKNNIYGTHVLLEACKVTGLIRRFIHVSTDEVYGETEKDAIEGNHEASQLLPTNPYSATKAGAEMLVMAYGRSYDLPVITTRGNNVYGPNQFPEKLIPKFILLAMQGKPLPIHGDGSNVRSYLYCEDVAEAFEVVLHKGEVGHVYNIGTQKERRVIDVAKDICRLFSLDAEKVIKFVDNRPFNDQRYYLDDKKLKDLGWSERTKWEEGLKKTMDWYIRQPDWWGDVSGALLPHPRMLTMAGVERYTEAHDVVDTTSPSPENGIEQNHMLERSNGLIPKSTLKFLIYGRNGWIGGLLGQICQKQGITYEYGNGRLENRSQILMDIQNIKPTHVFNAAGVTGRPNVDWCESHKPETITTNVAGTLNLADICKEQGLLLVNFATGCIFEYDDAHLEGSGIGFKEEDKPNFIGSFYSKTKAMVEDLLQNFENVCTLRVRMPISSDLSNPRNFITKITRYEKVVNIPNSMTVLDELLPISIEMAKRNCTGIWNFTNPGVVSHNEILQMYREYIDPTFKWTNFTLEEQAKVIVAPRSNNELNATKLKTEFPQLLSVKDSLLKYVFEPNRKNKLANGV*

Protein Length: 680

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATTTTGTTCCCTAGTGTGGGCATATTAAGTAGAGTTTGTCATTACAGACGTGTTTGTATGGGATTGGCTTTATTAAATTAAAGCTCTCACTAAATAAATCAGGAAATGCCAGGGAAGCCAGCAGGCTGAATCATTGGGCGCCCTATATATAAAGGCTGTGGTTTGCATCAATATTTGGGCCATAGGCATTGTTAGTGGGCTAACTCTGAGGTCACACAAAACCTTGAAGTCTTGTTTCTGTGGACCTCTGGATTGCTTTGCATCTGTCGAGGCTTCTGCTGTCAGTCCAAGATCTAGGTCTATTTGCATCCAATTTTAGAACGGGGTATTTTCTTTTATTCTTGATTGGCTGTTATTATTCAAACTGCGATTTGTTGGAAGA_-_ATGGAGAATGGCAGTGAAGGGTATGTGCCCAAGAACATTCTTATCACGGGTGCAGCAGGATTCATTGCCTCTCATGTAGCCAACAGGCTGGTGAGAAAATACCCACAATACAAGATTGTGGTATTGGACAAGCTGGATTATTGTTCGAATCTGAAGAATCTCCTGCCCTCCAGGTCTTCTCCCAATTTCAAATTTGTGAAGGGAGATATTGGGAGTGCAGATCTGGTAAATTATCTGCTGATCACAGAACAGATTGATACTATAATGCATTTTGCGGCACAAACCCATGTGGACAATTCATTTGGGAACAGCTTTGAGTTCACCAAGAACAACATCTATGGTACTCATGTCCTTCTTGAAGCATGCAAGGTGACTGGATTGATCAGAAGATTCATTCATGTGAGCACTGATGAAGTTTATGGTGAAACAGAGAAGGATGCCATTGAGGGAAATCATGAGGCCAGTCAGCTTTTACCCACTAATCCTTATTCTGCAACCAAGGCAGGAGCAGAGATGCTGGTCATGGCTTATGGGAGATCCTACGATTTGCCTGTGATCACCACAAGAGGTAACAATGTATATGGACCTAATCAGTTTCCTGAAAAACTGATACCCAAGTTCATTCTTCTGGCGATGCAAGGGAAGCCACTCCCAATCCATGGCGACGGATCCAATGTTAGGAGCTATTTGTACTGCGAGGACGTTGCTGAGGCTTTTGAAGTCGTTTTGCATAAAGGTGAAGTGGGGCATGTGTATAATATTGGTACACAAAAGGAGAGAAGGGTCATTGATGTGGCCAAGGATATCTGTAGGCTCTTTTCATTAGATGCAGAGAAGGTTATAAAGTTTGTAGACAACAGGCCTTTCAATGATCAGAGATATTATCTGGATGATAAGAAACTCAAGGATCTGGGATGGTCTGAGAGGACTAAATGGGAAGAGGGCTTGAAGAAAACAATGGATTGGTACATTAGACAACCTGATTGGTGGGGTGATGTGTCCGGTGCTCTGCTACCTCATCCAAGGATGCTCACAATGGCAGGAGTTGAGAGGTATACAGAAGCCCATGATGTGGTTGACACAACATCTCCCTCGCCAGAGAATGGCATCGAGCAGAATCACATGCTAGAGCGCTCCAACGGCCTTATTCCAAAGTCAACCTTGAAGTTCTTGATCTATGGGCGCAATGGCTGGATCGGAGGGCTTCTTGGGCAGATATGCCAAAAGCAGGGAATTACATATGAATATGGAAATGGGAGATTAGAGAATAGGTCTCAGATCCTAATGGACATACAGAACATCAAGCCTACTCATGTGTTTAATGCTGCAGGTGTGACAGGTCGGCCTAACGTGGATTGGTGTGAATCCCACAAGCCCGAGACTATTACAACCAATGTGGCGGGAACACTGAATTTGGCTGATATTTGCAAGGAGCAGGGTCTTTTGCTTGTAAATTTTGCAACTGGCTGTATTTTTGAATATGACGATGCTCATCTTGAAGGCAGTGGTATTGGATTTAAGGAGGAAGACAAACCCAACTTCATTGGGTCCTTTTATTCGAAGACCAAGGCCATGGTTGAAGATCTCCTACAAAATTTTGAAAATGTCTGTACTCTTAGGGTGAGAATGCCCATCTCCTCGGACCTGAGTAACCCTCGCAATTTCATCACAAAGATCACTCGATATGAGAAAGTGGTAAATATACCAAACAGTATGACAGTCCTTGATGAGTTGCTGCCAATCTCGATTGAGATGGCAAAAAGAAATTGCACTGGCATATGGAACTTCACTAACCCAGGTGTTGTGAGCCATAATGAAATCTTGCAGATGTACAGAGAATATATTGATCCCACTTTCAAGTGGACAAACTTTACTCTTGAAGAACAGGCAAAAGTCATAGTTGCTCCTCGAAGTAATAATGAACTGAATGCCACCAAGTTGAAGACGGAATTTCCACAGTTGCTGTCAGTAAAAGATTCATTATTGAAGTATGTCTTTGAGCCCAACAGGAAGAACAAGTTAGCCAATGGAGTTTAG___TCCTTGCTTAAGATCAAATTTTCATTAGGTGTAGTAAAGTTTTTCTCTGCTTAGGCAGGCAAAACATCCATATGCTTAATTGGGGGACAATTCAAAAGGTCGTCCAGTACTTTGTTATTTTTAGCTCACAAATGGAGGAGAGCTTATTTGGTAGGATATTCTAGATATGTTCTACTATTTTTCTTCTGATTTTACATTTTAGTTCACCAACGGAGGAGCCGACTAGCTAGGGTATTCTAGATAGGTTCTACAATTCTTGTTCTGTTTTTATATTTACATTCTTTCTATGAATGTAAGTCAACTTACAAGGTTAATGCCATTGAATTTCCGCTTCCAAGGAATTCTACTATTTGGAATATATTCGTCTTTGACAAGACTGTTCTTCTTGTATGAAAAAAGTTCTCACATTTTTATAAAATAACTGCTCAAGCTTTGTGTAGTT

Fln Alignment:

Contig5209___LKFLIYGRNGWIGGLLGQICQKQGITYEYGNGRLENRSQILMDIQNIKPTHVFNAAGVTGRPNVDWCESHKPETITTNVAGTLNLADICKEQGLLLVNFATGCIFEYDDAHLEGSGIGFKEEDKPNFIGSFYSKTKAMVEDLLQNFENVCTLRVRMPISSDLSNPRNFITKITRYEKVVNIPNSMTVLDELLPISIEMAKRNCTGIWNFTNPGVVSHNEILQMYREYIDPTFKWTNFTLEEQAKVIVAPRSNNELNATKLKTEFPQLLSVKDSLLKYVFEPNRK
A9NN20______________LKFLIYGRTGWIGGEIGKLCEAQGIAFEFGKGRLENRSQLQEDIEAVKPTHVFNAAGVTGRPNVDWCESHKVETIRTNVVGTLNLADLCRQHGLILINYATGCIFEYDEKHPLGSGIGFKEEDTPNFMGSYYSKTKAMVEDLLKNFENVCTLRVRMPISSDLSNPRNFITKISRYDKVVNIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILEMYRDYIDPSYTWRNFTLEEQAKVIVAARSNNEMDASKLKKEFPELLPIKESLVKYVFEPNQK

SNPs (tot: 25)

Position	A %	C %	G %	T %	Probability
2547	75		25		1.0
2550		31		68	1.0
2661	87			12	0.992554
2663		87	12		0.992554
2664		87		12	0.992554
2665		87	12		0.992554
2668	12			87	0.992554
2669	87		12		0.992554
2675			90	10	0.90298
2676	10	90			0.90298
2678		10		90	0.90298
2681		20		80	0.999996
2683		11	88		0.923564
2686	14	85			0.993533
2687	71	28			1.0
2688	42	14	14	28	1.0
2689	66		33		1.0
2693		20	20	60	0.99255
2697		33		66	0.997452
2701	66	33			0.997452
2704		66	33		0.997452
2705		66	33		0.997452
2706	66			33	0.997452
2711	33	66			0.997452
2713			33	66	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain