SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene5148

Length: 1404 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene5148

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	pfam00759, Glyco_hydro_9, Glycosyl hydrolase family 9	-	-	45%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	52%
Blast2go	endoglucanase 16-like	-	-	72%

Source	ECs	Term	Type	e value	Identity
Blast2go	Hydrolases, Glycosylases, Glycosidases, i.e. enzymes hydrolyzing O- and S-glycosyl compounds.	EC:3.2.1.-	-	0.0	72%

Source	KEGGs	Term	Identity
Blast2go	Ascorbate and aldarate metabolism	00053	72%
Blast2go	Ubiquinone and other terpenoid-quinone biosynthesis	00130	72%
Blast2go	Amino sugar and nucleotide sugar metabolism	00520	72%
Blast2go	Glycosaminoglycan degradation	00531	72%
Blast2go	Metabolic pathways	01100	72%

Source	GOs	Term	Type	Identity
Blast2go	metabolic process	GO:0008152	Biological Process	72%
Blast2go	hydrolase activity, hydrolyzing O-glycosyl compounds	GO:0004553	Molecular Function	72%
Blast2go	cytoplasmic membrane-bounded vesicle	GO:0016023	Cellular Component	72%

Source	InterPros	Term	Type	Identity
Sma3	Glycoside hydrolase, family 9	IPR001701	-	-
Sma3	Six-hairpin glycosidase	IPR012341	-	-
Sma3	NADH:ubiquinone oxidoreductase, B12 subunit	IPR012576	-	-
Sma3	Glycoside hydrolase, family 9, active site	IPR018221	-	-
Sma3	WD40 repeat, conserved site	IPR019775	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NUH0

Fln msg: your sequence is shorter than subject: 419 - 514

Fln protein: VGGYYDAGDNVKYGLPMAFTVTMLAWGALHYGNELAAAGELDNVRDAIRWGTDYFIKASSIDNELWVQVGDPYADHSCWQNPEIMDTPRSLYKIDPSTPGTEIAAETAAALAASSIVFVAIDNPYSLFLLDRAKSLFDFADQYRGTYDGECPFYCSFSGYDDELAWAASWLYRATRSPKYSQYILQQNFSTSNVSEFSWDLKSAGVQVLLSNLYFSGESIFEIYKNQAEMFFCSVLPFSPHFQHSMTPGGLLYVRDGANTQYATSTSFLLLTYSDLLSSHNQSLHCDYQIYRADDLIAFAKQQIHYILGWNPLGMSFMVGFGDRYPQQAHHRGSSIVSIYETTAKVSCTEGFMVWFRRQAPNPNELTGAVVGGPDRYDNFEDYRADSCKLEPTTYINAPLVGVLARLHTISMGSPVTT*

Protein Length: 420

Fln nts: GTTGGTGGTTACTACGATGCTGGGGATAATGTGAAGTATGGCCTTCCCATGGCATTCACCGTTACAATGTTAGCATGGGGAGCCCTTCACTATGGAAATGAACTTGCAGCAGCTGGAGAGTTGGACAACGTTAGGGATGCCATTAGGTGGGGTACTGACTATTTCATAAAAGCAAGCTCCATTGATAACGAGTTATGGGTTCAGGTGGGAGATCCATATGCAGATCATAGTTGTTGGCAAAACCCAGAGATAATGGACACTCCAAGGTCTTTGTACAAGATAGATCCATCCACTCCTGGAACAGAAATTGCAGCAGAGACTGCTGCAGCATTGGCTGCTTCCTCCATTGTCTTCGTAGCCATTGACAATCCTTATTCCCTTTTCCTCCTTGACAGAGCTAAATCGCTGTTTGATTTTGCTGACCAGTACCGTGGAACATATGATGGAGAATGCCCTTTCTACTGTTCTTTCTCAGGCTATGACGACGAGCTTGCATGGGCAGCGTCATGGCTGTATAGAGCAACCAGATCACCAAAGTATTCCCAATACATACTCCAGCAGAATTTCTCTACTTCCAATGTATCTGAGTTCAGCTGGGATCTCAAATCTGCGGGAGTTCAAGTCCTGCTCTCTAATCTATATTTCTCTGGAGAATCCATCTTTGAAATTTACAAAAACCAAGCAGAAATGTTCTTCTGCTCAGTTCTTCCATTTAGTCCTCACTTCCAGCATTCCATGACACCAGGAGGATTGCTGTATGTGAGAGATGGTGCAAATACACAATATGCTACAAGCACATCATTCCTCCTATTGACTTACAGTGACCTATTGTCAAGCCACAACCAATCCCTGCATTGTGATTATCAAATCTACCGGGCGGACGATCTTATTGCATTTGCCAAACAACAAATCCATTACATATTGGGCTGGAATCCACTGGGTATGTCTTTCATGGTGGGATTTGGTGACAGATATCCACAGCAAGCTCATCACAGAGGATCATCTATAGTTTCGATATACGAGACAACGGCAAAGGTATCATGCACAGAAGGTTTCATGGTGTGGTTCCGCAGACAAGCACCGAACCCCAATGAATTAACAGGTGCAGTTGTAGGAGGGCCTGATCGCTATGATAATTTTGAAGATTACAGGGCCGATTCGTGCAAGCTTGAGCCCACAACTTATATCAATGCTCCTCTCGTGGGGGTCCTTGCCAGGCTCCACACCATCAGCATGGGGTCTCCAGTTACAACTTAG___TAACTTCACCTAGATTTTTTGCTTGGTATTTGTTCTGGGCTGTTTTGCTCATTTCACCTTTGGATCTTACAGCCAGATTAAGTTTGATAGGCTTGTAAATCAATTTTTCTTCAATTTTATGAAAATAGAATTAAAGTTACGAATTTT

Fln Alignment:

Contig5148___GGYYDAGDNVKYGLPMAFTVTMLAWGALHYGNELAAAGELDNVRDAIRWGTDYFIKASSIDNELWVQVGDPYADHSCWQNPEIMDTPRSLYKIDPSTPGXXXXXXXXXXXXXXXXXXXXIDNPYSLFLLDRAKSLFDFADQYRGTYDGE-----CPFYCSFSGYDDELAWAASWLYRATRSPKYSQYILQ--QNFSTS-NVSEFSWDLKSAGVQVLLSN--LYFSGESIFEIYKNQAEMFFCSVLPFSPHFQHSMTPGGLLYVRDGANTQYATSTSFLLLTYSDLLSSHNQSLHCDYQIYRADDLIAFAKQQIHYILGWNPLGMSFMVGFGDRYPQQAHHRGSSIVSIYETTAKVSCTEGFMVWFRRQAPNPNELTGAVVGGPDRYDNFEDYRADSCKLEPTTYINAPLVGVLARL
A9NUH0______________GGYYDAGDNVKFGFPMAFTTTMLSWSVLEFGGRMG--DNLDHARAAIRWATDYLLKATAHPGVIYVQVGDPNMDHKCWERPEDMDTPRNVYKIDAEHPGSDIAAETAAALAAASMVFRATDPTYSGHLLQTAMRVFDFADKHRGAYSDSLQSEVCPFYCSYSGYNDELLWGAAWLQRASRNVSYISYIQSNGQNLGAEDNVNIFSWDEKHAGARVLLAKEVLLRNSKSLQE-YRGHADNFICSLLPGTQNSQAQYTPGGLLFKMSDNNLQYVTSTTFLLFTYAKYLTVSKQVVRCGNIIATPTRIRTLAKRQVDYILGDNPLGMSYMVGYGAKFPEHVHHRGSSLPSIYQHPRIIPCNDGFQSLY-SNAPNPNRLVGAVVGGPDNNDRFSDERNDYAHSEPTTYINAPLVGSLAYL

SNPs (tot: 8)

Position	A %	G %	T %	Probability
816	75	25		0.996473
841	75	25		0.996473
857	25		75	0.996473
1012	18	81		0.999994
1249		85	14	0.993533
1250		16	83	0.994512
1251		16	83	0.994512
1252	83	16		0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain