SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene5068

Length: 1495 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene5068 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	RTPHOS1_7_B09.g1_A029 Roots minus phosphorous Pinus taeda cDNA clone RTPHOS1_7_B09_A029 5', mRNA sequence.	-	-	98%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	37%
Blast2go	heat shock protein binding protein	-	-	73%

Source	GOs	Term	Type	Identity
Blast2go	heat shock protein binding	GO:0031072	Molecular Function	73%
Blast2go	vacuolar membrane	GO:0005774	Cellular Component	73%
Blast2go	mitochondrion	GO:0005739	Cellular Component	73%

Source	InterPros	Term	Type	e value	Identity
Sma3	Heat shock protein DnaJ, N-terminal	IPR001623	-	0.0	-
Sma3		IPR015609	-	0.0	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5ADZ5

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 182 aas, putative chimeric sequence, No stop codon before M and low homology subject, your sequence is shorter than subject: 260 - 286

Fln protein: MEGSCGKFRQQWEAAALKKRVAQGVKFRRYVVEEEASASGSNLEPDSLCREFEREGKESPHANLQDHYVDHCSADPPDLASTSGIIYTSGQEDVTSPITMNKSEIPNSDVETSDGVSLGQSINTVEVKPLRKENGCERNSFGVKMKEVLEFVHQKSHQGAKNSLKSTSGGAKKDSEMMEKMNGWEGDREKLKKTEEFKHAEQEEWARRHLELQRQSEEARRLWKRKREEVTRNREMERRQKQRLDEMRENQKRMKKQCI*

Protein Length: 261

Fln nts: CAAATATTTGAGACAGGTAACGTATTTACATCATTAAGAAATGTGAAAAGAATCCATAGGCAAAAGTCATTAGGAAAAGAGCCCGCTGATGATTATACAGATATATCTGAAAGTGCGACAAGTTCAGATGGTGATAGTTCAGACTGTGAAATC_-_ATGGAAGGGTCTTGTGGAAAATTTCGTCAGCAATGGGAAGCAGCAGCATTAAAAAAACGTGTTGCTCAGGGTGTCAAATTTAGAAGATATGTGGTTGAGGAGGAGGCTAGTGCTTCTGGTTCCAATCTTGAACCAGATAGCCTTTGCAGGGAATTTGAGCGAGAAGGTAAGGAGAGCCCTCATGCTAATTTACAAGATCACTATGTTGATCACTGTTCAGCAGATCCCCCCGATCTTGCAAGTACTAGTGGCATAATTTATACATCAGGACAGGAGGATGTGACCAGCCCTATAACCATGAATAAGTCTGAGATACCGAATTCTGATGTTGAGACCTCTGATGGTGTTTCACTTGGTCAATCTATAAATACAGTTGAGGTGAAACCATTGAGAAAAGAAAATGGATGTGAAAGGAACAGTTTTGGGGTGAAGATGAAAGAAGTTTTGGAGTTTGTTCATCAAAAATCTCATCAGGGGGCAAAAAATAGTCTGAAGTCAACTTCTGGTGGAGCCAAAAAAGACTCTGAAATGATGGAAAAAATGAATGGATGGGAAGGAGATCGGGAGAAGCTGAAAAAAACTGAAGAGTTCAAACATGCTGAACAAGAGGAATGGGCACGGAGGCACTTGGAGCTGCAGCGTCAGTCGGAAGAGGCAAGGCGATTGTGGAAGAGAAAAAGGGAGGAAGTTACTCGTAATCGGGAGATGGAGAGAAGGCAAAAGCAACGTCTTGATGAAATGCGAGAAAACCAAAAAAGGATGAAGAAACAATGCATTTGAAAGATCAGTTGCGTGGACAAGTAAGGGCAGAGCTTGAACAAGTTGCATCTCAATGCAAAGATATGGCATCATTGCTTCGCCATTTGGGCATACCTGTGGAAGGCGGAGCCTTTCCCCTGTCACAGCAGGTTAATGCAGCTTATAAGCAAGCTTTATTGCGTTTCCATCCAGATCGTGCATCGAAACGTGGTTTGCGTCAGCAGGTTGAGGCTGAGGAGACCTTTAAATTGATTTCACGCTTAAAGGACACACTGTTGCCAGTAGCTCCAATGTCCTTGAAGAGGACATCTTGGTGATGATTTGCTTTGTAATCTAATATCTCAACCATCTTGAATAGCACAGGGGCTATTGTCTTATGGTTTCACTCTGTATTATACCATGACAATGTGTCAAAGTCCTTTATATCTGGTTTTCTCTTTCCACAGGCAGACTATTTCTGCAACTATCAGAGCTTACATTTTTTCAAGTGACCTTTATATATGTTGCCTACATTCAGAAAATAATTGTAACTCTTTATGCTGCACATCTTCTAATTTGAAATACACTCTCGTGAGTATTATGG

Fln Alignment:

Contig5068___EGDREKLKKTEEFKHAE------QEEWARRHLELQRQSEEARRLWKRKREEVTRNREMERRQKQ------RLDEMRENQKRMKKQ
D5ADZ5______________EGEFERLKREREERLAELQAIRKQEREMRRKMEYYRRQEEARLIKLKEEEEARKREEEERKRKQDAEWKAKLDEMTEKQKQRERE

SNPs (tot: 43)

Position	A %	C %	G %	T %	Probability
221		71		14	0.984337
222		14		85	0.993533
278	10			90	0.999898
279		90	10		0.999898
280		90		10	0.999898
281			10	90	0.999898
283	10			90	0.999898
284			10	90	0.999898
290	90			10	0.999898
293		10		90	0.999898
296	10		90		0.999898
297	90	10			0.999898
364		9		90	0.999872
372	85		15		1.0
419			11	88	0.999952
421		11		88	0.999937
560		88		11	0.923564
561			11	88	0.923564
562	11			88	0.923564
564		88	11		0.923564
566	88	11			0.923564
568			11	88	0.923564
569		11	88		0.923564
572	11			88	0.923564
573			11	88	0.923564
576		12		87	0.992554
577	12			87	0.992554
579			12	87	0.992554
580	16			83	0.994512
582	83		16		0.994512
903	14			85	0.994477
904	14	85			0.994477
1061		60		40	1.0
1065		10		90	0.991528
1067	10	90			0.991528
1170	12			87	0.992554
1172	12	87			0.992554
1184		80		20	0.995493
1192		40	60		1.0
1272	60		40		1.0
1277		60	40		1.0
1312	11	88			0.923564
1400	83		16		0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain