SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene5056

Length: 1789 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene5056 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Chromatin remodeling complex subunit (Fragment) n=1 Tax=Populus trichocarpa RepID=B9H8S6_POPTR	-	-	54%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	28%
Blast2go	helicase-like transcription factor	-	-	69%

Source	GOs	Term	Type	Identity
Blast2go	ATP binding	GO:0005524	Molecular Function	69%
Blast2go	helicase activity	GO:0004386	Molecular Function	69%
Blast2go	DNA binding	GO:0003677	Molecular Function	69%
Blast2go	zinc ion binding	GO:0008270	Molecular Function	69%

Source	InterPros	Term	Type	Identity
Sma3	SNF2-related	IPR000330	-	-
Sma3	von Willebrand factor, type C	IPR001007	-	-
Sma3	Helicase, C-terminal	IPR001650	-	-
Sma3	Zinc finger, RING-type	IPR001841	-	-
Sma3	DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site	IPR002464	-	-
Sma3		IPR004348	-	-
Sma3	Helicase, superfamily 1/2, ATP-binding domain	IPR014001	-	-
Sma3		IPR014021	-	-
Sma3	HIP116, Rad5p N-terminal	IPR014905	-	-
Sma3	Zinc finger, RING-type, conserved site	IPR017907	-	-
Sma3	Zinc finger, C3HC4 RING-type	IPR018957	-	-

Full-Lengther Next Prediction

Fln status: Putative N-terminus

Fln database: coniferopsida.fasta

Fln subject: D5AD70

Fln msg: Overlapping hits, possible frame ERROR between 1210 and 1191, Unexpected STOP codon at 3' end. Distance to subject end: 16 aas, No stop codon before M and low homology subject, your sequence is longer than subject: 491 - 377

Fln protein: MKEANEISKFDIVLTTYSTLGSELNNLSSPIKEIKWLRIILDEAHFIKNAGTKQAKAAIALNAERRWAVTGTPIQNTAFDLFTLMAFLHFEPFSIKSYWNSLVQRPLSQGNKSGCSRLQALLETVALRRTKDMKINGQCLVELPPKYVQIHPVELSHEERELYNNVETEGRKVVQNFINSGTVLNNYSSILQIILRLRQICDDALLCPSDVESLFSSFKLEDASGNPNLLKKLLSVLQAGDDFDCPVCLSPPSEAIITICSHVFCKKCIEKILKHLKPQCPLCRKQLTTSDLFSSPKVDDENEVTSEKVVKTGSKINALISLLKESQDHDPTTKSVVFSQFRKMLDLLQEPLGxxxxxxxxRLDGSMPPKKRAASITAFMSNKPSSPTILLASLKAAGVGINLTAASDVYMFDPWWNPAVEDQAMDRIHRIGQTRSVRVLRLVVKDSIEERILEMQDGKRKLASSAFKDKSDKEQRKMRVEDVQRLMRLS*

Protein Length: 492

Fln nts: AGATCCCGATGGGCCCAGGGCAACCCTCGTTGTCTGCCCGCCCTCCGTGCTTTCGAACTGGGTGGATCAGCTCGAGGAACACACTAAGCGGGGAAGCTTGAAAGTTTATCTATACCATGGAGGTGGTCGC_-_ATGAAGGAAGCAAACGAAATATCTAAATTTGATATTGTTCTGACAACCTATAGTACCCTTGGTTCAGAATTAAATAATCTGAGCTCACCTATCAAGGAGATCAAGTGGTTGAGAATAATTTTGGATGAGGCACATTTCATAAAAAATGCTGGTACTAAACAAGCGAAGGCGGCAATAGCTTTAAATGCTGAAAGAAGGTGGGCCGTTACTGGTACTCCGATTCAAAACACGGCGTTTGATTTGTTTACACTGATGGCATTCCTGCATTTTGAACCATTTTCAATCAAATCCTATTGGAACAGCTTAGTTCAACGCCCGCTATCTCAAGGCAACAAGAGTGGTTGTTCACGCTTACAGGCTTTATTGGAGACCGTTGCTCTCCGAAGGACCAAAGATATGAAAATCAATGGACAGTGCTTAGTGGAACTTCCACCTAAATATGTGCAGATCCATCCGGTAGAACTGTCTCATGAGGAGCGTGAACTCTACAATAATGTGGAAACAGAAGGCAGAAAAGTGGTTCAAAACTTCATTAATTCAGGCACCGTGCTAAATAATTATTCTTCTATCCTTCAGATAATTCTACGGCTACGTCAAATTTGTGATGATGCATTGTTGTGCCCATCTGATGTGGAGTCATTGTTTTCGTCTTTCAAATTAGAAGATGCTTCAGGCAATCCCAATTTGCTGAAGAAATTACTTTCAGTTTTGCAAGCTGGAGATGACTTTGACTGTCCAGTATGTTTATCACCACCGTCAGAGGCTATCATAACCATCTGTTCACATGTGTTCTGTAAGAAGTGCATTGAGAAGATATTGAAACATCTAAAGCCACAGTGTCCATTGTGCCGTAAGCAGCTTACAACATCTGATCTTTTTAGTTCACCAAAGGTTGATGATGAGAATGAAGTTACATCAGAAAAAGTAGTCAAAACTGGTTCAAAAATTAATGCATTAATATCTCTATTGAAAGAGTCCCAGGATCATGATCCAACTACAAAATCTGTTGTATTTTCACAGTTTCGAAAAATGCTGGATCTCTTGCAGGAACCTTTGGGAxxxxxxxxxxxxxxxxxxxxxxxxCGTTTGGATGGGTCTATGCCTCCAAAGAAGCGAGCTGCATCCATTACTGCATTCATGTCAAACAAACCAAGCAGCCCAACCATCCTCTTGGCAAGTTTGAAAGCTGCAGGAGTAGGGATAAATCTAACTGCTGCTTCTGATGTCTACATGTTTGACCCTTGGTGGAATCCTGCCGTGGAAGATCAAGCAATGGATCGAATACACAGAATTGGGCAGACAAGGTCTGTGAGAGTGCTCAGGTTAGTTGTAAAGGACAGCATTGAGGAGCGCATATTAGAGATGCAAGATGGGAAACGGAAGTTAGCTAGCTCTGCATTCAAAGACAAGTCTGACAAGGAGCAACGCAAAATGCGTGTGGAAGATGTACAGCGTCTTATGCGGTTATCATAGATTGAATTCGTGTAGTGTAATTCTACACTTCGTTTTTGTAGGTGTCAAGATCGTGACTGCCTTTTGGCCTGAATAGGGTAATTCATTGTCCAGATGAAGAATACTTGTGATTCCCCAAGCATTTTGTACCAAGGAATATTCAAGTCAAATATTCCTTTTGGATATTATTCTATTTTACATAAGATAAC

Fln Alignment:

Contig5056___RKQLTTSDLFSSPKVDDENEVTSEKVVKTGSKINALISLLKESQDHDPTTKSVVFSQFRKMLDLLQEPLGxxxxxxxRLDGSMPPKKRAASITAFMSNKPSSPT-ILLASLKAAGVGINLTAASDVYMFDPWWNPAVEDQAMDRIHRIGQTRSVRVLRLVVKDSIEERILEMQDGKRKL
D5AD70______________RKNFNHPDLLQSQFEISYHYPPVEKLMEQCGKFQLLDRLLKHLRARNH--KVLIFSQWTRVLDLLDYCLSxxxxxxxRIDGSVKLHDRQRQIKDF--NDPNSNLHIFLLSTRAGGLGINLTAADTCIIYDSDWNPQMDLQAMDRCHRIGQTKPVHVYRLCTSHSAECRMLKVAFDKLKL

SNPs (tot: 4)

Position	A %	C %	G %	T %	Probability
1474	33			66	0.997452
1478			66	33	0.997452
1479		66		33	0.997452
1609	33		66		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain